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Plant Sciences

Theses/Dissertations

Louisiana State University

Sugarcane

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Cover Cropping: Sensor-Based Estimations Of Biomass Yield And Nutrient Uptake And Its Impact On Sugarcane Productivity, Hector H. Fajardo Duran Jul 2022

Cover Cropping: Sensor-Based Estimations Of Biomass Yield And Nutrient Uptake And Its Impact On Sugarcane Productivity, Hector H. Fajardo Duran

LSU Master's Theses

Sugarcane in Louisiana can be harvested for up to three years from one planting. Soil cultivation along sides of established beds is done for weed control and improve fertilizer use efficiency which increases the risk of soil degradation and yield decline. Planting cover crops (CC) is a soil conservation practice and an effective strategy to improve soil health and nutrient recycling. Limited work has been done on remote sensor-based evaluation of the potential nutrient benefits from cover crops and its effect on nutrient cycling on sugarcane systems. This study was conducted to evaluate the effect of two planting methods (broadcast …


Evaluating The Effect Of Coated Urea And Urea-Ammonium-Nitrate Solution On Cane Tonnage, Sugar Yield, And Leaf-Soil Nitrogen Content Under Louisiana Sugarcane Production System, Hector J. Mendoza Lagos May 2022

Evaluating The Effect Of Coated Urea And Urea-Ammonium-Nitrate Solution On Cane Tonnage, Sugar Yield, And Leaf-Soil Nitrogen Content Under Louisiana Sugarcane Production System, Hector J. Mendoza Lagos

LSU Master's Theses

Nitrogen (N) is a crucial nutrient for proper plant growth and development. N deficiency results in poor tillering and thin, short internode stem production for sugarcane production, thus resulting in low cane tonnage. Therefore, proper N fertilizer management is essential for a sustainable sugarcane production system. This research was conducted at the LSU AgCenter Sugar Research Station in St. Gabriel, LA, from 2019 to 2020 at three Sites (Site 1, 2, and 3). The treatments consisted of different N rates (0, 45, 90, and 135 kg N ha-1) and sources (urea-ammonium-nitrate solution [UAN]-32%, 30-day [Coated Urea 1], 45-day …


Examination Of Pre-Emergence Control Of Johnsongrass In Sugarcane Seedlings, Carleton Baucum Apr 2022

Examination Of Pre-Emergence Control Of Johnsongrass In Sugarcane Seedlings, Carleton Baucum

LSU Master's Theses

Sugarcane is susceptible to many diseases and insect pests; therefore, to maintain high sugar yield new varieties must be developed. Producing new varieties contributes to the overall crop success through enhanced yield, insect and/or disease resistance, cold tolerance, and ratooning ability. However, unlike conventional sugarcane, which is vegetatively propagated, new sugarcane varieties are produced from true seed. These seedlings are more susceptible to herbicidal injury and weed competition than conventional sugarcane. For sugarcane seedlings to succeed, weeds must be controlled therefore, the most effective herbicide program with regards to seedling safety must be implemented. The objectives of this research were …


Marker-Trait Association Mapping Of Sorghum Mosaic Virus Resistance In Sugarcane, Chenie Zamora Apr 2022

Marker-Trait Association Mapping Of Sorghum Mosaic Virus Resistance In Sugarcane, Chenie Zamora

LSU Master's Theses

Mosaic is a potentially devastating disease of sugarcane that had severe economic impact on Louisiana’s sugarcane industry in the 1920’s and has caused periodic problems ever since. The disease is vectored by migrating aphids that transmit Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV) in a non-persistent manner. Mosaic in Louisiana is currently caused by strains of SrMV. Mosaic has been controlled primarily by developing resistant cultivars. Recently, mosaic was detected in some near-commercial clones in the cultivar selection program. This prompted extensive screening of breeding program parent populations that detected infiltration of susceptibility and revealed the need for …


Integrated Nutrient Management And Cover Cropping Practices In Louisiana Sugarcane Production Systems, Daniel Erneso Forestieri Oct 2021

Integrated Nutrient Management And Cover Cropping Practices In Louisiana Sugarcane Production Systems, Daniel Erneso Forestieri

LSU Doctoral Dissertations

Integrating effective fertilizer management with conservation practices is essential to improve farm income and promote environment friendly sugarcane production systems. A study was conducted in multiple to document the impact of nitrogen (N) source and cover cropping on sugarcane yield and quality components. The treatment consisted of different N sources: urea, knife-in urea ammonium nitrate (UAN) solution, calcium nitrate-CaNO3, ammonium sulfate-NH4SO4, and knife-in UAN. + foliar N, applied at 90 kg ha-1 and control (0 N). The cover crops included: crimson clover (Trifolium incarnatum), hairy vetch (Vicia villosa), and oilseed radish (Raphanus sativus L.) planted at 13, 17, and 1 …


Distribution And Incidence Of Mosaic And Evaluation Of Susceptibility In Louisiana's Current Sugarcane Germplasm, Jancee Rice Oct 2018

Distribution And Incidence Of Mosaic And Evaluation Of Susceptibility In Louisiana's Current Sugarcane Germplasm, Jancee Rice

LSU Master's Theses

Mosaic is a viral disease of sugarcane caused primarily by Sorghum mosaic virus (SrMV) in Louisiana. Low mosaic incidence has resulted from successful breeding for resistance. However, mosaic was detected in breeding program experimental clones and a new cultivar, HoCP 09-804.Therefore, multiple research approaches were undertaken to assess the current status of mosaic in Louisiana sugarcane and prevent it from re-emerging as an important problem. Field surveys conducted during 2016-2018 of breeding program yield trials and experimental clone seed-cane increases determined disease incidence and distribution. Mosaic was detected in three of five sugarcane production areas and incidence ranged from 0 …


Genome Wide Association Studies For Identification Of Markers Linked To Sucrose Traits In Sugarcane, Nathanael Darrell Fickett Oct 2018

Genome Wide Association Studies For Identification Of Markers Linked To Sucrose Traits In Sugarcane, Nathanael Darrell Fickett

LSU Doctoral Dissertations

Sugarcane, being a complex aneu-polyploid, poses unique challenges to fine mapping of quantitative trait loci (QTL) controlling agronomic traits of interest. Genome-wide association studies (GWAS) could be a better alternative to identify molecular markers associated with specific traits due to high linkage disequilibrium in sugarcane. In the first study, marker-trait associations (MTAs) were evaluated for three sucrose traits, Brix, total recoverable sugar (TRS), and percent sucrose on 48 elite and historic Louisiana breeding clones using 1,062 alleles. These sucrose traits were highly correlated (P-value < 0.0001) at >0.96. TASSEL 5.1 and JMP Genomics 8.0 were compared with eight models run in each program. …


Evaluation Of Controlled-Release Nitrogen And Potassium Fertilizer In Louisiana Sugarcane Production, Samuel Kwakye Apr 2018

Evaluation Of Controlled-Release Nitrogen And Potassium Fertilizer In Louisiana Sugarcane Production, Samuel Kwakye

LSU Master's Theses

Sugarcane (Saccharum spp) production is an integral part of Louisiana's economy. Proper fertilizer management, particularly nitrogen (N) and potassium (K), optimizes sugarcane production. An experiment was established in 2015 at the LSU AgCenter Sugar Research Station in St. Gabriel, Louisiana to: (1) assess the effect of N and K fertilizer sources on sugarcane yield and quality parameters, and (2) monitor the changes in inorganic N and K levels in the soil at different sugarcane growth stages. The experiment was arranged in a randomized complete block design on a Sharkey clay soil and complete randomized design on a Commerce …


A Comparison Of Soils And Their Associated Microbial Communities As Affected By Sugarcane Cultivation, Adam Francis Bigott Aug 2017

A Comparison Of Soils And Their Associated Microbial Communities As Affected By Sugarcane Cultivation, Adam Francis Bigott

LSU Master's Theses

In Louisiana, sugarcane has been grown in the same soils for over 200 years. A phenomenon wherein soils with a long-term sugarcane cropping history produce decreased yields compared to adjacent land without a recent history of sugarcane cultivation has been documented in multiple sugarcane growing regions. Research in both Louisiana and internationally has shown positive plant growth responses when soils with a long-term cultivation history are sterilized or treated with selective biocides, suggesting there is a biological component to the underlying soil health issue. In this study, soil microbial ecology was compared for paired sites with short and long-term sugarcane …


Improving Nitrogen Use Efficiency And Yield In Louisiana Sugarcane Production Systems, Daniel Ernesto Forestieri Jan 2017

Improving Nitrogen Use Efficiency And Yield In Louisiana Sugarcane Production Systems, Daniel Ernesto Forestieri

LSU Master's Theses

Proper nitrogen (N) management is essential to optimize crop production. This study was conducted to evaluate different N fertilizer management strategies to improve N use efficiency and yield in sugarcane production in Louisiana. This research was initiated in 2013 at the Sugar Research Station in St. Gabriel, LA and was arranged in a randomized complete block design with four replications consisting of different N rates (0, 45, 90, and 135 kg N ha-1) and sources (urea-46% N, ammonium nitrate [AN]-34% N, and urea-ammonium-nitrate solution [UAN]-32% N dribbled and knifed-in) as treatments. Sensor readings were taken from different N response trials …


Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa Jan 2016

Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa

LSU Doctoral Dissertations

Development of resistant cultivars is the main control measure against sugarcane brown rust caused by Puccinia melanocephala. Durability is uncertain, since the pathogen possesses adaptive ability to overcome host plant resistance. A differential gene expression study utilizing suppressive subtraction hybridization was conducted to improve understanding of brown rust resistance mechanisms in sugarcane. The expression patterns of 11 unigenes representing biosynthetic pathways, defense-related genes, and signaling genes were analyzed in L 99-233, a cultivar exhibiting quantitative resistance, L 01-299, a resistant cultivar with the major resistance gene Bru1, and two susceptible cultivars, Ho 95-988 and L 09-125, at 24 h, 48 …


Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros Jan 2016

Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros

LSU Doctoral Dissertations

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide. The disease is managed primarily with resistant cultivars obtained through classical breeding; however, the erratic symptom expression hinders the reliability and reproducibility of the selection process. The development of molecular markers associated with incompatible/compatible reaction can overcome this limitation. Suppression subtractive hybridization (SSH) and quantitative trait locus (QTL) mapping were the strategies used to find leaf scald resistance-associated genes and molecular markers in sugarcane. SSH results showed that genes involved in signal perception and transduction, and DNA binding, were highly expressed in the resistant clone LCP 85-384 compared …


Screening For Resistance To Sugarcane Brown Rust With Controlled Conditions Inoculation, Mavir Carolina Avellaneda Barbosa Jan 2014

Screening For Resistance To Sugarcane Brown Rust With Controlled Conditions Inoculation, Mavir Carolina Avellaneda Barbosa

LSU Master's Theses

Brown rust, caused by Puccinia melanocephala, is an important disease of sugarcane. Breeding for host plant resistance is the primary control measure. Screening for resistance has relied on rating the severity of symptoms caused by natural infection; however, erratic results make this method problematic. A method accomplishing both infection and disease expression under controlled conditions could avoid the problems associated with resistance evaluations under natural infection. Inoculation of seedlings was evaluated to determine whether it could provide accurate resistance ratings in cross appraisal, and inoculation under controlled conditions was evaluated for the potential to accurately determine resistance reactions in clones …


Detection And Quantification Of Xanthomonas Albilineans In Sugarcane Tissues With Quantitative Polymerase Chain Reaction And Evaluation As Methodology For Monitoring Resistance, Andres Felipe Gutierrez Viveros Jan 2014

Detection And Quantification Of Xanthomonas Albilineans In Sugarcane Tissues With Quantitative Polymerase Chain Reaction And Evaluation As Methodology For Monitoring Resistance, Andres Felipe Gutierrez Viveros

LSU Master's Theses

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide that is controlled primarily with host plant resistance. Since visual evaluation of disease resistance can be uncertain due to erratic symptom expression, a more reliable screening method is needed for resistance research. A quantitative polymerase chain reaction (qPCR) assay was developed previously with demonstrated potential for resistance screening. However, only four cultivars with extreme reaction against the disease (two highly susceptible and two highly resistant) were compared. Therefore, additional research was conducted to demonstrate assay specificity, compare bacterial populations in 31 clones with varying levels of susceptibility at …


Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando Jan 2011

Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando

LSU Doctoral Dissertations

Leaf scald is an important disease of sugarcane caused by Xanthomonas albilineans (Xa). Leaf scald is controlled by the development and planting of resistant cultivars. However, erratic symptom expression makes conventional screening for resistance difficult. In addition, the mechanisms of resistance to leaf scald are not completely understood. Real-time, quantitative polymerase-chain-reaction (qPCR) assays were developed utilizing SYBR Green for a highly sensitive detection method or a TaqMan probe to quantify Xa populations in infected plants. Xa populations detected by qPCR followed similar trends to disease severity ratings and vascular infection results for two resistant and two susceptible cultivars under greenhouse …


Sugarcane Seed Response To 2,4-D And Alternative Herbicides For Red Morningglory (Ipomoea Coccinea L.) Control, Jonathan Daniel Siebert Jan 2003

Sugarcane Seed Response To 2,4-D And Alternative Herbicides For Red Morningglory (Ipomoea Coccinea L.) Control, Jonathan Daniel Siebert

LSU Master's Theses

Field studies conducted over two growing seasons evaluated the effect of 2,4-D applied at 1.6 kg ai/ha to LCP 85-384 sugarcane (Saccharum interspecific hybrid) 7, 5, 3, and 1 wk before planting (WBP). Sugarcane was planted in mid-September using both whole stalk and billet (45 cm) seed pieces. When 2,4-D was applied 5 wk or closer to planting, sugarcane shoot emergence and population averaged across planting methods was reduced 5, 7, and 28 wk after planting (WAP) when compared to the nontreated control. Sugarcane height in one of two years was reduced when 2,4-D was applied 5 wk or closer …