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Plant Sciences

Theses/Dissertations

Louisiana State University

QPCR

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Sweetpotato Virus C And Its Contribution To The Potyvirus Complex In Sweetpotato (Ipomoea Batatas), Favio E. Herrera Eguez Nov 2017

Sweetpotato Virus C And Its Contribution To The Potyvirus Complex In Sweetpotato (Ipomoea Batatas), Favio E. Herrera Eguez

LSU Doctoral Dissertations

In Louisiana, sweetpotato (Ipomoea batatas) is infected in Louisiana by the four ubiquitous potyviruses: Sweetpotato feathery mottle virus (SPFMV), Sweetpotato virus G (SPVG), Sweetpotato virus 2 (SPV2) and the strain of SPFMV previously known as the common strain, recently renamed as Sweetpotato virus C (SPVC). These four viruses belong to the Potyviridae family, with single stranded RNA of ~11kb. In this group of plant viruses, a single polyprotein is coded entirely but later cleaved into ten mature proteins: P1, HC-pro, P3, 6K1, CI, 6K2, NIa-VPg, NIa-Pro Nib and Coat Protein (CP). In sweetpotato potyviruses, two additional open reading …


Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva Jan 2014

Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva

LSU Master's Theses

Cercospora leaf blight (CLB) of soybean caused by Cercospora kikuchii is an important disease in Lousiana. Preliminary screening of foliar applications of the micronutrients Fe, Mn, Cu, Zn, B, Mo and Al, showed that Fe decreased CLB severity consistently. The objective of this work was to test the effects of commercial formulations of Fe, Manny Plex Fe and Fe EDTA (Brandt Consolidated, Springfield, IL) on leaf colonization by C. kikuchii, symptom development (blight and purple leaves), and yield. Four rates of Manny Plex Fe and four rates of Fe EDTA were applied to field plots at R5 growth stage. Leaf …


Detection And Quantification Of Xanthomonas Albilineans In Sugarcane Tissues With Quantitative Polymerase Chain Reaction And Evaluation As Methodology For Monitoring Resistance, Andres Felipe Gutierrez Viveros Jan 2014

Detection And Quantification Of Xanthomonas Albilineans In Sugarcane Tissues With Quantitative Polymerase Chain Reaction And Evaluation As Methodology For Monitoring Resistance, Andres Felipe Gutierrez Viveros

LSU Master's Theses

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide that is controlled primarily with host plant resistance. Since visual evaluation of disease resistance can be uncertain due to erratic symptom expression, a more reliable screening method is needed for resistance research. A quantitative polymerase chain reaction (qPCR) assay was developed previously with demonstrated potential for resistance screening. However, only four cultivars with extreme reaction against the disease (two highly susceptible and two highly resistant) were compared. Therefore, additional research was conducted to demonstrate assay specificity, compare bacterial populations in 31 clones with varying levels of susceptibility at …


Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando Jan 2011

Comparative Proteome And Qpcr Analysis Of The Sugarcane Reaction To Leaf Scald Caused By Xanthomonas Albilineans, Freddy Fernando Garces-Obando

LSU Doctoral Dissertations

Leaf scald is an important disease of sugarcane caused by Xanthomonas albilineans (Xa). Leaf scald is controlled by the development and planting of resistant cultivars. However, erratic symptom expression makes conventional screening for resistance difficult. In addition, the mechanisms of resistance to leaf scald are not completely understood. Real-time, quantitative polymerase-chain-reaction (qPCR) assays were developed utilizing SYBR Green for a highly sensitive detection method or a TaqMan probe to quantify Xa populations in infected plants. Xa populations detected by qPCR followed similar trends to disease severity ratings and vascular infection results for two resistant and two susceptible cultivars under greenhouse …