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Plant Sciences

Theses/Dissertations

Louisiana State University

Cercosporin

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Dsrna As A Potential Tool For Cercospora Leaf Blight Management And Elucidation Of A Factors That Contribute To Late Disease Onset In The Reproductive Growth Stage Of Soybean, Marija Zivanovic Jul 2020

Dsrna As A Potential Tool For Cercospora Leaf Blight Management And Elucidation Of A Factors That Contribute To Late Disease Onset In The Reproductive Growth Stage Of Soybean, Marija Zivanovic

LSU Doctoral Dissertations

Cercospora leaf blight (CLB) of soybean (Glycine max L. Merr.), an important disease in Louisiana, is primarily caused by Cercospora cf. flagellaris. The pathogen produces cercosporin, a toxin necessary for fungal virulence. So far, there are few commercial cultivars of soybean with CLB resistance and few effective fungicides to manage this disease. Consequently, alternative management approaches are needed. With that in mind, the first objective was to develop a screening method to compare the effectiveness of bacterially-expressed double stranded (ds) RNAs corresponding to genes associated with cercosporin accumulation (Avr4, CTB1, CTB8, AY-1 and HN-2 …


Documentation Of Siderophore Activity, Metal Binding, And Its Effect On Symptomatology Of Cercospora Leaf Blight Caused In Soybean By Cercosporin From Cercospora Cf. Flagellaris, Brian Michael Ward Jan 2017

Documentation Of Siderophore Activity, Metal Binding, And Its Effect On Symptomatology Of Cercospora Leaf Blight Caused In Soybean By Cercosporin From Cercospora Cf. Flagellaris, Brian Michael Ward

LSU Doctoral Dissertations

Cercospora leaf blight of soybean (Glycine max) is a serious problem in the mid-south of the United States and is present in most soybean-growing regions of the world. The causal organisms, Cercospora kikuchii, C. cf. flagellaris and C. cf. sigesbeckiae, utilize the photo-activated toxin cercosporin as the primary pathogenicity factor. The disease has historically been understood to progress from a purpling or bronzing of the leaves to a blight in which tissue dies. Previous literature showed possible binding of cercosporin to metals and previous work in our group showed possible use of minor element nutrition in the plant to reduce …


The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende Jan 2017

The Roles Of Avr4 In Fungal Virulence,Cercosporin Biosynthesis And Its Potential Use In Host Induced Gene Silencing For Controlling Cercospora Leaf Blight Disease Of Soybeans, Josielle Santos Rezende

LSU Doctoral Dissertations

The AVR4 effector, secreted by Cladosporium fulvum, has been demonstrated to be involved in pathogen virulence. Recent studies further demonstrated that Avr4 is highly conserved among several Cercospora species, indicating a potential important role of this gene in fungal virulence. Therefore, investigation to determine whether this fungal effector gene is present in Cercospora cf. flagellaris (previously known as C. kikuchii), the causal agent of soybean cercospora leaf blight (CLB) disease, and whether it plays any role in CLB disease development, is of great interest. In the present study, the Avr4 gene from C. cf. flagellaris was cloned and mutants lacking …


Effects Of Minor Elements On Cercospora Kikuchii, Cercospora Leaf Blight And Rust On Soybeans, Brian Michael Ward Jan 2015

Effects Of Minor Elements On Cercospora Kikuchii, Cercospora Leaf Blight And Rust On Soybeans, Brian Michael Ward

LSU Master's Theses

Soybean (Glycine max) is one of the most widely grown crops in the world. Many pathogens attack soybeans, but of particular importance to tropical and subtropical areas such as Louisiana is Cercospora leaf blight (CLB). This disease is caused by the fungus Cercospora kikuchii and favored by high temperatures. This fungus utilizes a toxin, cercosporin, as its primary pathogenicity factor. Soybean rust (SBR), caused by Phakopsora pachyrhizi, is another common disease that occurs in Louisiana under cooler environmental conditions of spring and fall. Both diseases occur regularly in Louisiana and may result in severe yield losses. Cercospora kikuchii has recently …


Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva Jan 2014

Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva

LSU Master's Theses

Cercospora leaf blight (CLB) of soybean caused by Cercospora kikuchii is an important disease in Lousiana. Preliminary screening of foliar applications of the micronutrients Fe, Mn, Cu, Zn, B, Mo and Al, showed that Fe decreased CLB severity consistently. The objective of this work was to test the effects of commercial formulations of Fe, Manny Plex Fe and Fe EDTA (Brandt Consolidated, Springfield, IL) on leaf colonization by C. kikuchii, symptom development (blight and purple leaves), and yield. Four rates of Manny Plex Fe and four rates of Fe EDTA were applied to field plots at R5 growth stage. Leaf …


Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda Jan 2012

Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda

LSU Doctoral Dissertations

Cercospora leaf blight (CLB) caused by Cercospora kikuchii, has become a troublesome disease in the southern United States. C. kikuchii produces a non-hostspecific phytotoxin and a pathogenicity factor known as cercosporin during infection of soybean. A quantitative real-time PCR assay was developed for detection and quantification of C. kikuchii. The sensitivity of detection is 1 pg of genomic DNA. The assay detected the presence of C. kikuchii in soybean leaves long before the appearance of disease symptoms. C. kikuchii DNA levels in soybean leaves increased slowly during early soybean development, followed by a quick increase at late reproductive stages. Results …