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Identification Of Blda As A Major Regulator Of Virulence In The Sweetpotato Soil Rot Pathogen Streptomyces Ipomoeae And Characterization Of Distinct Clostridium Sweetpotato Soft Rot Isolates, Kuei-Ting Yang

LSU Doctoral Dissertations

Streptomyces ipomoeae is the causative agent of Streptomyces soil rot on sweetpotato, a disease characterized by extensive necrosis of both adventitious and storage roots. While Streptomyces potato scab pathogens produce a phytotoxin (thaxtomin A), which is induced in the presence of cellobiose and suberin, S. ipomoeae produces a less-modified, phytotoxin (thaxtomin C), whose inducer has not been identified. To investigate transcriptional regulation of thaxtomin C production, we inserted a promoter involved in thaxtomin C synthesis upstream of the gusA reporter in an S. ipomoeae strain. Reporter gene expression was significantly upregulated in the presence of size-fractionated sweetpotato extract (SPE). The …

Differential Lead Accumulation In Brassica Juncea, Brassica Rapa, And Lactuca Sativa And Evaluation Of Ground Level Barriers To Prevent Lead Contamination Of Produce Grown In Raised Beds, Celine Vera Richard

LSU Master's Theses

High lead (Pb) contamination of soils is a threat to human health. Urban area soils are frequently contaminated with lead from settling of gasoline exhaust, brake dust, and lead paint on homes, old playground equipment, etc. Exposure to soil-lead occurs by ingestion or inhalation and poses an elevated risk for young children. Indirect ingestion can occur through the food chain through activities such as growing certain vegetables with an ability to tolerate and accumulate lead in edible tissues. Many university extension systems recommend growing vegetables in raised beds as a reasonable reduced risk option for avoiding lead accumulation in garden …

Molecular Mechanisms Of Boron Toxicity Tolerance In Plants, Guannan Wang

LSU Doctoral Dissertations

Boron toxicity is a worldwide agricultural problem that limits crop productivity and quality. However, our understanding on the genetic responses and adaption mechanisms to boron toxicity in plants is very limited. To address this gap in our knowledge, I compared boron stress-sensitive model, Arabidopsis thaliana and its stress-adapted relative Schrenkiella parvula to study how plants respond and adapt to excess boron at physiological, genomic, transcriptomic, and metabolic levels.

The overall project goal involved integration of multi-omics datasets to develop genome to phenome interpretations. To achieve this, I developed a python package, GOMCL, to facilitate the extraction of biologically meaningful information …

Evaluation Of Louisiana Farm To School Programming: Harvest Of The Month And Local Food Procurement, Arin M. Shaffer

LSU Master's Theses

Farm to school is a growing movement that is beginning to draw serious interest in different parts of the United States due to its ability to connect communities with their local producers while providing many benefits. Farm to school entails three main components: school gardening, education and curriculum, and local food procurement. The farm to school movement is relatively new to the state of Louisiana and very little research has been conducted to evaluate the impact of the farm to school programs on local food procurement and the effect on school food environments. Case studies of three Louisiana parish school …

Within-Field Yield Prediction For Sugarcane And Rice Focused On Precision Agriculture Applications, Felippe Hoffmann Silva Karp

LSU Master's Theses

Food and energy security are two main topics when it comes to the on-growing world population. Rice and sugarcane play an important role in this scenario since sugarcane can be used for energy production and rice is one of major staple cereals. In this scenario, Precision Agriculture (PA) management strategies aims to improve productivity, efficiency, profitability, and sustainability, and can help agriculture to fulfill the needs of the growing population in a sustainable way. However, yield maps are essential for PA, but its adoption is still very low. Thus, the main objective of this study was to evaluate the potential …

Boxwood Dieback: Molecular Detection, In-Vitro Fungicide Efficacy, And Host Susceptibility For Managing A New Emerging Disease Caused By Colletotrichum Theobromicola, Harleen Kaur

LSU Master's Theses

Boxwood (Buxus spp. L) is one of the most common and widely planted perennial ornamentals in both home gardens and commercial landscapes. Grown for its evergreen, dark green foliage, boxwood cultivation dates back to 4000 BC in Egypt. Although considered hardy, boxwood is susceptible to several plant pathogens. Recently reported boxwood dieback, a fungal disease caused by Colletotrichum theobromicola, has been spreading at an alarming rate within the United States. Boxwood dieback consists of symptoms that resemble to those caused by Phytophthora root rot, Volutella blight, and some abiotic disorders and can be easily misdiagnosed in nurseries and …

Ecology Of The Roseau Cane Scale (Nipponaclerda Biwakoensis, Hemiptera: Aclerdidae) In Coastal Louisiana, Leslie Alejandra Aviles Lopez

LSU Master's Theses

Common reed, Phragmites australis, is the dominant plant in the Mississippi River Delta (MRD), Louisiana. Phragmites australis stands reduce soil erosion from wave action, protect the oil infrastructure, and freshwater habitats. In the fall of 2016, widespread reed die-backs in the MRD were associated with outbreaks of an invasive scale Nipponaclerda biwakoensis (Hemiptera: Aclerdidae). Due to the recent detection of the scale, there was limited knowledge of its ecology in the adventive range, and its impacts on P. australis lineages. Therefore, the objectives of my thesis were to determine (1) the host specificity of the N. biwakoensis in important economic …

Floodplain Forest Regeneration Dynamics In The Lower Mississippi River Alluvial Valley, Whitney Anne Kroschel

LSU Doctoral Dissertations

Floodplain forest species diversity is driven, in part, by variation in disturbance regime. Flood patterns create heterogeneity in microsite quality from small differences in elevation across a floodplain which, in turn, influence flood timing and duration. Differences in species’ regeneration niches in relation to hydrologic patterns can account for long-term coexistence of various species. In the past century floodplain forests have exhibited a wide range of changes in stand development and species composition as a result of altered hydrology in rivers and floodplains. I evaluated the role of regeneration in floodplain forest systems of the Lower Mississippi River Alluvial Valley …

Dsrna As A Potential Tool For Cercospora Leaf Blight Management And Elucidation Of A Factors That Contribute To Late Disease Onset In The Reproductive Growth Stage Of Soybean, Marija Zivanovic

LSU Doctoral Dissertations

Cercospora leaf blight (CLB) of soybean (Glycine max L. Merr.), an important disease in Louisiana, is primarily caused by Cercospora cf. flagellaris. The pathogen produces cercosporin, a toxin necessary for fungal virulence. So far, there are few commercial cultivars of soybean with CLB resistance and few effective fungicides to manage this disease. Consequently, alternative management approaches are needed. With that in mind, the first objective was to develop a screening method to compare the effectiveness of bacterially-expressed double stranded (ds) RNAs corresponding to genes associated with cercosporin accumulation (Avr4, CTB1, CTB8, AY-1 and HN-2 …

Estimating The Onset And Extent Of Dieback Of Phragmites Australis Using The Normalized Difference Vegetation Index And Remotely Sensed Land Cover Classifications, Aimee M. Beaudette

LSU Master's Theses

Phragmites australis is cosmopolitan plant species with an invasive variety present throughout most of North America. In the Balize Delta, Louisiana, USA, P. australis plays an important role in combatting subsidence, maintaining navigation channels, and protecting interior fish and wildlife habitat from waves and storm surge. In 2016 a dieback of P. australis was reported by wetland managers, coinciding with the appearance of an invasive Asian scale insect (Nipponaclerda biwakoensis), though the specific cause is still unknown. Two previous efforts attempted to identify the onset of dieback conditions met with limited success. Using Landsat images from 1985 to …

Cover Cropping In Soybean-Corn Rotation System: Economic, Agronomic, And Soil Fertility Impact, Jose Rodolfo Mite

LSU Doctoral Dissertations

Planting winter-annual cover crops prevent soil erosion, reduces water runoff, and improves soil structure and soil quality. This research was conducted from 2017 to 2019 to evaluate the nutrient turnover of different species of cover crops in soils under different row crop production systems in Northeast and Central Louisiana. In Northeast Louisiana (Site 1, 2, and 3), treatments (cover crops and no cover crop) were arranged in a strip trial with three replications. At the Ben Hur Research Station, the treatments included three planting dates (September, October, and November) with [7 kg ha^-1 of phosphorus (P) and potassium (K)] …

Larval Survival And Movement Of The Fall Armyworm And Corn Earworm (Lepidoptera: Noctuidae) In Seed Blends Of Non-Bt And Pyramided Bt Corn: Implications For Resistance Management, Marcelo Dimase

LSU Master's Theses

A seed blend refuge has been used in the U.S. Corn Belt for Bt corn insect resistance management (IRM). The fall armyworm, Spodoptera frugiperda (F.), and corn earworm, Helicoverpa zea (Boddie) are major target pests of Bt corn in the Americas. One major concern for the use of a seed blend refuge is that larval movement of insects among non-Bt and Bt plants could affect its efficacy for resistance management. To address this concern, field and greenhouse trials were conducted to determine 1) if seed blend refugia could provide comparable levels of susceptible S. frugiperda (aabb) as structured refugia and …

Functions Of Cdk/Cyclin Complexes In Endoreplication Regulation By The Cdk Inhibitor Siamese, Kai Wang

LSU Doctoral Dissertations

Arabidopsis trichome (leaf hair) is a specialized single cell extended from epidermal cell on the leaves, which is a typical endoreplication and is also known as endoreduplication. Several D-type cyclins were tested to check the cell division in trichome, and the trichome expressing either CYCB1;2 or CDKB2;2 cannot trigger cell division, even if simultaneous expression of CYCB1;2 and CDKB2;2 failed to produce mitosis in trichome. Only CYCD3;1 specifically promotes multicellular trichome. cdkb1;1cdbk1;2 double mutants and sim cdkb1;1cdkb1;2 triple mutants exhibit the phenotype similar to the wild type and very limited cell division respectively. Overexpression of a CDKB1;1 dominant-negative construct that …

Phenotypic And Genotypic Characterization Of Aroma And Cercospora Janseana Resistance In U.S. Rice, Christopher Keith Addison

LSU Doctoral Dissertations

Rice is a staple food for over one-third of the world’s population. With the increasing population, plant breeders must continue to breed for improved varieties. The utilization of markers to applied breeding program can increase the efficiency of selection for key traits. The targeted traits for this research include grain aroma and resistance to the fungal disease narrow brown leaf spot (Cercospora janseana). Our objective was to characterize SNP diversity across the BADH2 gene in publicly available sequence data of a collection of 2,932 rice varieties was characterized to determine the number of gene haplotypes in O. sativa. …

Soil Health On A Small-Scale Sustainable Vegetable Farm In South Louisiana, Allison Guidroz

LSU Master's Theses

One of the biggest challenges facing the world today is the need to provide nutritious food to an ever-growing population in a way that does not compromise the ecosystem services of the soil that are necessary for life. The evidence for widespread degradation of the soil has been a major contributor in the increased interest in soil health. Most of the research in soil health has focused on field crops, which has led to a gap in the research of soil health on vegetable cropping systems. This objective of this study was to analyze the health of the soil on …