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Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus Jun 2016

Discovering Potential Protein, Carbohydrate, And Lipid Based Food Ingredients In A Co-Culture Of Microalgae, Chelsea Tyus

LSU Doctoral Dissertations

Louisiana Native Co-Culture of Microalgae (Chlorella Vulgaris L. and Cyanobacteria Leptolyngbya sp.) (CCA) was studied. CCA is a viable coculture for further investigation as a food component. This research characterized the proteins, carbohydrates, and lipids present in CCA. Algae cultivation parameters were controlled and analyzed. Treatment (Trt) 1 was CCA grown in cultures exposed to average scalar irradiance (ASI) of 1041 ± 269.18 μmol m-2 s-1 PAR and trt 2 was CCA grown in cultures exposed to ASI of 430 ± 96.03 μmol m-2 s-1 PAR. The trt (irradiance exposure) had the desired response on CCA species ratio Trt …


Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa Jan 2016

Identification Of Genes Associated With Resistance To Brown Rust In Sugarcane And Prevalence Of One Major Gene, Mavir Carolina Avellaneda Barbosa

LSU Doctoral Dissertations

Development of resistant cultivars is the main control measure against sugarcane brown rust caused by Puccinia melanocephala. Durability is uncertain, since the pathogen possesses adaptive ability to overcome host plant resistance. A differential gene expression study utilizing suppressive subtraction hybridization was conducted to improve understanding of brown rust resistance mechanisms in sugarcane. The expression patterns of 11 unigenes representing biosynthetic pathways, defense-related genes, and signaling genes were analyzed in L 99-233, a cultivar exhibiting quantitative resistance, L 01-299, a resistant cultivar with the major resistance gene Bru1, and two susceptible cultivars, Ho 95-988 and L 09-125, at 24 h, 48 …


Molecular Genetics Of Salinity Tolerance In Rice (Oryza Sativa L.), Teresa Bermejo De Leon Jan 2016

Molecular Genetics Of Salinity Tolerance In Rice (Oryza Sativa L.), Teresa Bermejo De Leon

LSU Doctoral Dissertations

Due to the threat of salinity stress to Louisiana rice production, an effort was made to understand the molecular genetics of salinity tolerance with the overall goal of developing salt tolerant varieties. The objectives of this study were to 1) determine if salinity tolerance exist in the US rice varieties, 2) map the additive and epistatic QTLs for traits related to seedling salinity tolerance in recombinant inbred lines (RILs) using genotyping-by-sequencing (GBS)-derived SNP markers, and 3) identify and validate stable QTLs and their effects in introgression lines (ILs) of Pokkali in Bengal background. All experiments for phenotypic characterization were conducted …


Geographic Distribution And Severity Of Strobilurin Fungicide Resistance Among Rhizoctonia Solani On Rice In Southwestern Louisiana, Allysson Gayle Lunos Jan 2016

Geographic Distribution And Severity Of Strobilurin Fungicide Resistance Among Rhizoctonia Solani On Rice In Southwestern Louisiana, Allysson Gayle Lunos

LSU Master's Theses

Strobilurin fungicide resistance threatens rice production in southwestern Louisiana. Strobilurins are the often relied upon method of managing sheath blight development due to the lack of strong genetic resistance to this disease in the currently available rice germplasm. However, isolates of Rhizoctonia solani AG 1-IA causing sheath blight were reported resistant to azoxystrobin near Mowata, LA in 2011. To determine the geographic distribution and severity of azoxystrobin resistance, 40 commercial rice fields in southwestern Louisiana were sampled for isolates of R. solani. Sensitivity to pyraclostrobin and trifloxystrobin was also assessed to investigate cross-resistance. A genetic test was used to …


Evaluation Of Ammonia Volatilization And Zinc Nutrition Of Experimental Zinc Sulfate Coated Urea Fertilizers In A Drill-Seeded Delayed Flood Rice Production System, Nutifafa Adotey Jan 2016

Evaluation Of Ammonia Volatilization And Zinc Nutrition Of Experimental Zinc Sulfate Coated Urea Fertilizers In A Drill-Seeded Delayed Flood Rice Production System, Nutifafa Adotey

LSU Doctoral Dissertations

Approaches in controlling nitrogen (N) loss from preflood fertilizer applications in delayed flood rice (Oryza stiva) production in the mid-southern U.S.A. typically involves treating urea with urease inhibitors like N-(n-butyl) thiophosphoric triamide (NBPT). Limited research exists on managing N utilizing the combined effects of treating urea with a urease inhibitor and subsequently adding a physical zinc (Zn) sulfate coating. Zinc deficiency is also a major soil fertility constraint in flooded rice production. The objectives of this study were to: (i) determine the ammonia volatilization potential of experimental zinc sulfate coated urea (ZSCU) fertilizers on four rice soils in a controlled …


Persistent Rna Viruses Of Common Bean (Phaseolus Vulgaris): Distribution And Interaction With The Host And Acute Plant Viruses, Surasak Khankhum Jan 2016

Persistent Rna Viruses Of Common Bean (Phaseolus Vulgaris): Distribution And Interaction With The Host And Acute Plant Viruses, Surasak Khankhum

LSU Doctoral Dissertations

Common bean (Phaseolus vulgaris) is the most important legume for direct human consumption. Common bean originated and was domesticated in the Americas but now is grown worldwide. As in the case of other crops, common bean can be infected with acute and persistent plant viruses. A modified dsRNA extraction method was developed and used in this study. The method was fast, economic, versatile, and required relatively small amounts of desiccated plant tissue. The method was successfully used to extract dsRNAs from plants infected with RNA plant viruses and to investigate the occurrence of two endornaviruses, Phaseolus vulgaris endornavirus 1 (PvEV1) …


Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros Jan 2016

Development Of Functional Markers For Resistance To Leaf Scald In Sugarcane, Andres Felipe Gutierrez Viveros

LSU Doctoral Dissertations

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide. The disease is managed primarily with resistant cultivars obtained through classical breeding; however, the erratic symptom expression hinders the reliability and reproducibility of the selection process. The development of molecular markers associated with incompatible/compatible reaction can overcome this limitation. Suppression subtractive hybridization (SSH) and quantitative trait locus (QTL) mapping were the strategies used to find leaf scald resistance-associated genes and molecular markers in sugarcane. SSH results showed that genes involved in signal perception and transduction, and DNA binding, were highly expressed in the resistant clone LCP 85-384 compared …


Integration Of Optical Remote Sensor-Based Yield Prediction And Impact Of Nitrogen Fertilization, Harvest Date, And Planting Scheme On Yield, Quality, And Biomass Chemical Composition In Energy Cane Production In Louisiana, Marilyn Sebial Dalen Jan 2016

Integration Of Optical Remote Sensor-Based Yield Prediction And Impact Of Nitrogen Fertilization, Harvest Date, And Planting Scheme On Yield, Quality, And Biomass Chemical Composition In Energy Cane Production In Louisiana, Marilyn Sebial Dalen

LSU Doctoral Dissertations

The established sugarcane industry in Louisiana is perceived as an advantage for biofuel industry because of the similarities of energy cane and sugarcane by way they are cultivated, harvested, and processed. This study was conducted at the LSU AgCenter Sugar Research Station in St. Gabriel, LA from 2013-2015 to evaluate the influence of planting scheme, N rate, and harvest date on energy cane yield, quality parameters, nutrient uptake, and biomass chemical composition. The relationship of vegetation indices (VI) with stalk, fiber yield, and N uptake of energy cane harvested at different dates was also evaluated. The experiments consisted of variety …


Effects Of Glyphosate On Soybean Nutrition, Endophytic Colonization By Cercospora Cf. Flagellaris And Development Of Cercospora Leaf Blight, Teddy Garcia Aroca Jan 2016

Effects Of Glyphosate On Soybean Nutrition, Endophytic Colonization By Cercospora Cf. Flagellaris And Development Of Cercospora Leaf Blight, Teddy Garcia Aroca

LSU Master's Theses

Glyphosate (Roundup®, Monsanto, Inc., St. Louis, MO) is the most widely used herbicide in the world because of its broad spectrum and its efficacy in controlling annual broadleaf weeds and undesired grasses. The effect of glyphosate on mineral nutrition and plant diseases has been an important topic during the past decade, because of its controversial effects on plant mineral nutrition. In order to test the hypothesis that glyphosate affects soybean mineral nutrition, and therefore predisposes soybean to Cercospora leaf blight, six glyphosate-resistant (GR) soybean varieties were either not treated or treated with glyphosate in field experiments. Plants were then evaluated …


Combining Forage And Cover Crop Benefits From Cool-Season Annuals In The Southeastern United States, Dustin John Smith Jan 2016

Combining Forage And Cover Crop Benefits From Cool-Season Annuals In The Southeastern United States, Dustin John Smith

LSU Master's Theses

Cow-calf operations in the southeastern United States (U.S.) are based on warm-season perennial grass pastures. Stored forage feeding during the non-grazing season constitutes more than half of a cattle operation’s annual expenses. Cool-season annuals can extend the grazing season, thereby reducing stored forage feeding. A two-year field trial was conducted to determine the forage potential of a variety of crops commonly used as winter cover crops in the southeastern U.S. The ten cover crop treatments included seven monocultures (annual ryegrass [Lolium multiflorum], rye [Secale cereal], oats [Avena sativa], triticale [Triticale hexaploide], tillage radish [Raphanus sativus], hairy vetch [Vicia villosa], crimson …


Influence Of Fertilizer Source And Irrigation Regimen On Containerized Production Of Coleus (Plectranthus Scutellarioides (L.) Codd) 'Solar Sunrise' And Management Of Bermudagrass (Cynodon Dactylon (L.) Pers. X C. Transvaalensis Burtt-Davy) 'Tifway', Kayla Renee Sanders Jan 2016

Influence Of Fertilizer Source And Irrigation Regimen On Containerized Production Of Coleus (Plectranthus Scutellarioides (L.) Codd) 'Solar Sunrise' And Management Of Bermudagrass (Cynodon Dactylon (L.) Pers. X C. Transvaalensis Burtt-Davy) 'Tifway', Kayla Renee Sanders

LSU Master's Theses

Fertilizer nutrient losses through leachate and runoff from excessive irrigation in nursery container production and turfgrass management can be high and have negative environmental impacts. The objective of this research was to examine the influence of fertilizer source and irrigation regimen on nutrient losses during nursery container production and turfgrass management. During the container production of coleus (Plectranthus scutellarioides (L.) Codd) 'Solar Sunrise', four fertilizer treatments: an unfertilized control; a controlled-release (CRF); a water-soluble (WSF); and a combination of 10% WSF and 90% CRF, were incorporated into a pine bark substrate at 0.30 kg N and P·m-3 in 3.7-L containers …


Influence Of Silicon On The Development Of Anthracnose Of Grain Sorghum, Sanjay Pokhrel Jan 2016

Influence Of Silicon On The Development Of Anthracnose Of Grain Sorghum, Sanjay Pokhrel

LSU Master's Theses

To study the effect of Silicon (Si) with and without a fungicide on anthracnose development of sorghum, several experiments were conducted in the greenhouse and field. In the initial study, different Si rates [0 (control), 0 (lime control), 200, 400, 600, 800 kg Si/ha] were used in a low-Si Alfisol to determine if inoculum densities were affected by Si levels. No differences were observed between inoculum densities of 1*105 and 1*106 conidia/ml in affecting anthracnose development in the greenhouse. Anthracnose severity was found to be lowest in plants treated with 800 kg Si/ha, regardless of inoculum density. In another study, …


Characterization Of Xylella Fastidiosa In Rabbiteye Blueberry, Mary Helen Ferguson Jan 2016

Characterization Of Xylella Fastidiosa In Rabbiteye Blueberry, Mary Helen Ferguson

LSU Doctoral Dissertations

Xylella fastidiosa colonizes the xylem of a wide range of plants and causes symptoms in many of them. It was of interest to determine how widespread X. fastidiosa was and what its potential impact might be in rabbiteye blueberry. X. fastidiosa was detected from two of 17 Louisiana orchards from which samples were collected. A yield study was conducted at one of these orchards, and mean yields of X. fastidiosa-positive plants were 55% and 62% less than those of X. fastidiosa-negative plants in 2013 and 2014, respectively. Average berry weight was also lower in X. fastidiosa-positive plants. However, plants that …