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Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva Jan 2014

Effects Of Iron On Cercospora Leaf Blight Of Soybean, Eduardo Chagas Ferreira Da Silva

LSU Master's Theses

Cercospora leaf blight (CLB) of soybean caused by Cercospora kikuchii is an important disease in Lousiana. Preliminary screening of foliar applications of the micronutrients Fe, Mn, Cu, Zn, B, Mo and Al, showed that Fe decreased CLB severity consistently. The objective of this work was to test the effects of commercial formulations of Fe, Manny Plex Fe and Fe EDTA (Brandt Consolidated, Springfield, IL) on leaf colonization by C. kikuchii, symptom development (blight and purple leaves), and yield. Four rates of Manny Plex Fe and four rates of Fe EDTA were applied to field plots at R5 growth stage. Leaf …


Screening For Resistance To Sugarcane Brown Rust With Controlled Conditions Inoculation, Mavir Carolina Avellaneda Barbosa Jan 2014

Screening For Resistance To Sugarcane Brown Rust With Controlled Conditions Inoculation, Mavir Carolina Avellaneda Barbosa

LSU Master's Theses

Brown rust, caused by Puccinia melanocephala, is an important disease of sugarcane. Breeding for host plant resistance is the primary control measure. Screening for resistance has relied on rating the severity of symptoms caused by natural infection; however, erratic results make this method problematic. A method accomplishing both infection and disease expression under controlled conditions could avoid the problems associated with resistance evaluations under natural infection. Inoculation of seedlings was evaluated to determine whether it could provide accurate resistance ratings in cross appraisal, and inoculation under controlled conditions was evaluated for the potential to accurately determine resistance reactions in clones …


Detection And Quantification Of Xanthomonas Albilineans In Sugarcane Tissues With Quantitative Polymerase Chain Reaction And Evaluation As Methodology For Monitoring Resistance, Andres Felipe Gutierrez Viveros Jan 2014

Detection And Quantification Of Xanthomonas Albilineans In Sugarcane Tissues With Quantitative Polymerase Chain Reaction And Evaluation As Methodology For Monitoring Resistance, Andres Felipe Gutierrez Viveros

LSU Master's Theses

Leaf scald, caused by Xanthomonas albilineans, is a major sugarcane disease worldwide that is controlled primarily with host plant resistance. Since visual evaluation of disease resistance can be uncertain due to erratic symptom expression, a more reliable screening method is needed for resistance research. A quantitative polymerase chain reaction (qPCR) assay was developed previously with demonstrated potential for resistance screening. However, only four cultivars with extreme reaction against the disease (two highly susceptible and two highly resistant) were compared. Therefore, additional research was conducted to demonstrate assay specificity, compare bacterial populations in 31 clones with varying levels of susceptibility at …


Development Of A Loop-Mediated Isothermal Amplification For The Detection Of Burkholderia Glumae, Maria Alejandra Caldera Jan 2014

Development Of A Loop-Mediated Isothermal Amplification For The Detection Of Burkholderia Glumae, Maria Alejandra Caldera

LSU Master's Theses

Bacterial panicle blight is among the three most limiting diseases of rice production in the United States. Yield loss up to 40% has been reported from rice fields severely infested with bacterial panicle blight. The disease is caused by Burkholderia glumae and B. gladioli. Although, both species produce similar symptoms and are closely related, but B. glumae strains are more aggressive and cause more severe symptoms on rice. Bacterial panicle bight is difficult to manage in the absence of effective chemical control measures. Rapid and early disease detection is needed to avoid severe yield losses. Several techniques have been developed …


Genetics And Genomics Studies Of Rice Disease Resistance And Development Of Alternative Disease Management Methods For Bacterial Panicle Blight And Sheath Blight, Bishnu Kumar Shrestha Jan 2014

Genetics And Genomics Studies Of Rice Disease Resistance And Development Of Alternative Disease Management Methods For Bacterial Panicle Blight And Sheath Blight, Bishnu Kumar Shrestha

LSU Doctoral Dissertations

Bacterial panicle blight (BPB) and sheath blight (SB), caused by the bacteria Burkholderia glumae and B. gladioli, and the fungus Rhizoctonia solani, respectively, are two major rice diseases in southern rice growing regions of US. No completely resistant rice cultivars have been identified for these diseases. However, a medium-grain cultivar, Jupiter, showed partial resistance to BPB. In order to understand the mechanisms of rice resistance against BPB and SB, rice genetics and genomics studies have been conducted. Alternative methods to suppress BPB and SB were also studied. Broad-sense heritability and correlations were calculated for the traits, BPB and SB disease …


Is The Foliar Yellow Vein Of Some Ornamental Plants Caused By Plant Viruses?, Favio Eduardo Herrera Jan 2014

Is The Foliar Yellow Vein Of Some Ornamental Plants Caused By Plant Viruses?, Favio Eduardo Herrera

LSU Master's Theses

Plant viruses have shown to be the cause of the yellow vein symptoms on the foliage of several ornamental plants. In this study five ornamental plants: oxalis (Oxalis debilis var. corymbosa) cv. Golden Veined Oxalis, geranium (Pelargonium peltatum) cv. Crocodile, pseuderanthemum (Pseuderanthemum carruthersii) cv. Golden Net Bush (narrow leaf and broad leaf variants), honeysuckle (Lonicera japonica) cv. Yellow Net Honeysuckle and coleus (Solenostemon scutellarioides) cv. Electric Lime, showing foliar yellow vein were used. Graft transmissions indicated that, except for coleus, the foliar yellow vein of all other plants was caused by infectious agents, likely plant viruses. With the exception of …


Characterization Of A Σ54-Dependent Response Regulator, Tepr, In The Rice-Pathogenic Bacterium Burkholderia Glumae And Development Of Biocontrol Strategies For Bacterial Panicle Blight Of Rice, Surendra Osti Jan 2014

Characterization Of A Σ54-Dependent Response Regulator, Tepr, In The Rice-Pathogenic Bacterium Burkholderia Glumae And Development Of Biocontrol Strategies For Bacterial Panicle Blight Of Rice, Surendra Osti

LSU Master's Theses

Bacterial panicle blight, caused by Burkholderia glumae, is a major bacterial disease of rice in Louisiana. This bacterium contains several virulence factors required for disease development such as toxoflavin, lipase and flagella. In a genome-wide search for regulatory factors related to the virulence of B. glumae, tepR was identified as a negative regulator for toxoflavin production and found to encode a sigma 54-dependent response regulator. TepR is homologous to LuxO, a quorum-sensing signaling component of Vibrio spp. A markerless tepR deletion mutant of B. glumae 336gr-1, LSUPB401, produced more toxoflavin and showed higher lipase and protease activities compared to the …


Characterization Of A Novel Negative Regulator Of Toxoflavin Production, Ntpr, In The Plant Pathogen Burkholderia Glumae That Causes Bacterial Panicle Blight Of Rice, Rebecca Ann Melanson Jan 2014

Characterization Of A Novel Negative Regulator Of Toxoflavin Production, Ntpr, In The Plant Pathogen Burkholderia Glumae That Causes Bacterial Panicle Blight Of Rice, Rebecca Ann Melanson

LSU Doctoral Dissertations

Burkholderia glumae is the major causal agent of bacterial panicle blight of rice, a disease that can cause significant yield losses. This bacterial pathogen produces a number of virulence factors that contribute to disease development. The phytotoxin, toxoflavin is required for full virulence and is the most important virulence factor in B. glumae. The known regulatory cascade of toxoflavin production involves the TofI-TofR quorum-sensing system, the transcriptional activators ToxJ and ToxR, and the toxoflavin biosynthesis and transport genes. Transposon mutagenesis of toxoflavin-producing B. glumae strains generated a number of mutants with increased toxoflavin production. Several of these mutants contained …


Species Abundance And Influence Of Nematodes In Urban Turfgrass Ecosystems In East Baton Rouge Parish, Louisiana, Addison Ray Plaisance Jan 2014

Species Abundance And Influence Of Nematodes In Urban Turfgrass Ecosystems In East Baton Rouge Parish, Louisiana, Addison Ray Plaisance

LSU Master's Theses

In 2011 and 2012, 100 residential lawns in the Baton Rouge, LA area were sampled to document the incidence of plant-parasitic nematodes, in addition four full-season microplot experiments and four 71-day duration greenhouse experiments were conducted to evaluate their pathogenicity on St. Augustine and centipede turfgrasses. Nematode genera associated with both turfgrasses included Criconemella, Helicotylenchus, Meloidogyne, Pratylenchus, Tylenchorynchus and Tylenchus spp. In microplot trials in 2012, nematodes did not cause significant damage to either turfgrass, but soil exhibited an effect on plant growth parameters. In 2013, when there was significant nematode related injury to both turfgrasses, there were no significant …