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Development Of Alternative Materials And Strategies For Enhancing Rice Health, Jobelle S. Bruno
Development Of Alternative Materials And Strategies For Enhancing Rice Health, Jobelle S. Bruno
LSU Master's Theses
Bacterial panicle blight (BPB) and sheath blight (ShB) are economically important diseases of rice (Oryza sativa) in Louisiana and other rice-growing regions around the world. However, there are challenges in the current disease management practices for these diseases. Thus, the main goal of this study is to identify new materials and their application methods that effectively suppress BPB and ShB. To achieve this goal, we evaluated the effect of various materials and application methods against the rice diseases. In 2020 and 2021, various materials, such as ascorbic acid (AA), N-acetylcysteine, chitosan, the avirulent strain of Burkholderia glumae 257sh-1 …
Development Of Biological Tools To Promote Rice Health And Growth, Ateet Maharjan
Development Of Biological Tools To Promote Rice Health And Growth, Ateet Maharjan
LSU Master's Theses
Sheath blight (ShB - caused by Rhizoctonia solani) and bacterial panicle blight (BPB - caused by Burkholderia glumae) are economically important rice diseases in Louisiana and other rice-growing regions. Fungicides and oxolinic acid are used to manage ShB and BPB, respectively, but these chemical methods are not sustainable economically and ecologically. Besides diseases, plant utilizable nitrogen (N) in the soil is inadequate for optimum crop yield, leading to the use of inorganic fertilizers and making agriculture less sustainable. To develop alternative biological control methods for protecting rice plants from diseases, rice-associated bacteria were screened based on their antagonistic …
Characterization Of A Σ54-Dependent Response Regulator, Tepr, In The Rice-Pathogenic Bacterium Burkholderia Glumae And Development Of Biocontrol Strategies For Bacterial Panicle Blight Of Rice, Surendra Osti
LSU Master's Theses
Bacterial panicle blight, caused by Burkholderia glumae, is a major bacterial disease of rice in Louisiana. This bacterium contains several virulence factors required for disease development such as toxoflavin, lipase and flagella. In a genome-wide search for regulatory factors related to the virulence of B. glumae, tepR was identified as a negative regulator for toxoflavin production and found to encode a sigma 54-dependent response regulator. TepR is homologous to LuxO, a quorum-sensing signaling component of Vibrio spp. A markerless tepR deletion mutant of B. glumae 336gr-1, LSUPB401, produced more toxoflavin and showed higher lipase and protease activities compared to the …
Comparative Genomics, Transcriptome Analysis And Characterization Of Selected Regulatory Genes Of Burkholderia Glumae, Felix Francis
Comparative Genomics, Transcriptome Analysis And Characterization Of Selected Regulatory Genes Of Burkholderia Glumae, Felix Francis
LSU Master's Theses
Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice, which is becoming a major threat to global rice production. The genome of a highly virulent B. glumae strain, 336gr-1 that was isolated from rice in Louisiana, was sequenced to better understand the genome-scale characteristics, particularly that of its pathogenicity. Comparative genomic analyses with another strain, BGR1 that was isolated from Korea, revealed several unique regions present in the genomes of these two geographically separated phytopathogenic bacteria. Genome plasticity, primarily caused by a horizontal gene transfer, was observed in these closely related strains of Burkholderia that are …
Identification Of Bacterial Pathogens Causing Panicle Blight Of Rice In Louisiana, Xianglong Yuan
Identification Of Bacterial Pathogens Causing Panicle Blight Of Rice In Louisiana, Xianglong Yuan
LSU Master's Theses
Four hundred and two bacterial isolates isolated on the semi-specific S-PG medium from diseased rice tissues showing symptoms of panicle blighting. These isolates were purified using serial dilution in sterile water and replating on S-PG medium. A total of 420 single isolates were obtained. These isolates were subjected to pathogenicity tests on rice (Oryza sative L. cv. Cypress). Based on these tests, 339 isolates were used in BiologTM tests and identified to the species level. Bacterial strains from 39 species in 16 genera were identified, including 52 isolates representing15 Pseudomonas species and 261 isolates representing six Burkholderia species. The remaining …