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Microdialysis: A Method For Quantifying In Situ Nitrogen Fluxes In Soil Microsites, Srusti Maddala, Mary C. Savin, Julie A. Stenken, Lisa S. Wood
Microdialysis: A Method For Quantifying In Situ Nitrogen Fluxes In Soil Microsites, Srusti Maddala, Mary C. Savin, Julie A. Stenken, Lisa S. Wood
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Microdialysis, a diffusion-based sampling technique commonly used in biomedical research, has recently been recognized as a candidate for monitoring chemical changes in the rhizosphere. The information it provides about nutrient diffusion may improve nitrogen use efficiency, leading to enhanced management and success of restoration projects. The objective of this study was to determine the efficacy of microdialysis sampling to quantify the relative recoveries (RR%) of nitrate-N and ammonium-N, the two inorganic nitrogen compounds typically found in soil. The effects of microdialysis flow rate, sample medium concentration, and the presence of both analytes in solution on the relative recoveries obtained from …
Short-Term Denitrification In The Metalimnion Of A Eutrophic Reservoir, Aki Kogo, Erin M. Grantz, Thad Scott
Short-Term Denitrification In The Metalimnion Of A Eutrophic Reservoir, Aki Kogo, Erin M. Grantz, Thad Scott
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Denitrification in metalimnetic water was examined under different conditions to determine how addition of nitrate (NO3 -) and particulate carbon (PC), aeration, and concentrations of nitrate affect denitrification. In the first experiment, water samples from a metalimnion were treated with different combinations of NO3 - and PC. Changes in excess N2 -N concentration for 10 days were measured using membrane inlet mass spectrometry (MIMS). The second experiment used the same treatments as the first experiment after aeration of water. Besides excess N2 -N concentrations, O2 concentrations were measured for 16 days. The third experiment examined how different initial concentrations of …
Bradyrhizobium Japonicum And Soybean Symbiotic Response To Glyphosate In Glyphosate-Tolerant Soybeans, Jodie M. Scheele, C. Andy King, Marilynn K. Davies, Larry C. Purcell
Bradyrhizobium Japonicum And Soybean Symbiotic Response To Glyphosate In Glyphosate-Tolerant Soybeans, Jodie M. Scheele, C. Andy King, Marilynn K. Davies, Larry C. Purcell
Discovery, The Student Journal of Dale Bumpers College of Agricultural, Food and Life Sciences
Soybean (Glycine max) grain contains approximately 40% protein and 6.5% nitrogen (N) on an elemental basis. Therefore, the plant requires an abundant N supply throughout its life cycle, and symbiotic N fixation of soybean with Bradyrhizobium japonicum provides 40 to 85% of the soybean N. Although soybean cultivars have been genetically engineered to withstand the herbicide glyphosate, B. japonicum grown in culture is sensitive to glyphosate. We hypothesized that glyphosate applied to glyphosate-tolerant soybean would inhibit nodulation by B. japonicum unless B. japonicum could also be selected for glyphosate tolerance. Cultures of B. japonicum were challenged with sublethal doses of …