Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Entire DC Network
Protein And Endotoxin Interactions And Endotoxin Removal From Protein Solutions, Liping Li
Protein And Endotoxin Interactions And Endotoxin Removal From Protein Solutions, Liping Li
Dissertations
The investigation objectives of this study include: endotoxin disaggregation by proteins; protein concentration effect on protein-endotoxin binding and endotoxin removal; size exclusion high performance liquid chromatography (SE-HPLC) studies on endotoxin dissociation by bovin serum albumin (BSA); use of Ca+2 to re-aggregate endotoxin in hemoglobin solutions and the subsequent removal of endotoxin by ultrafiltration; quantitative determination of Ca+2 effects on endotoxin removal and protein yield in a two-stage ultrafiltration.
It is known that some proteins can disaggregate endotoxins and form complexes with lipopolysaccharides (LPS). It is also known that Ca can act as "bridges" to aggregate LPS in aqueous …