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Development And Integration Of Informatic Tools For Qualitative And Quantitative Characterization Of Proteomic Datasets Generated By Tandem Mass Spectrometry, Rachel Michelle Adams
Development And Integration Of Informatic Tools For Qualitative And Quantitative Characterization Of Proteomic Datasets Generated By Tandem Mass Spectrometry, Rachel Michelle Adams
Doctoral Dissertations
Shotgun proteomic experiments provide qualitative and quantitative analytical information from biological samples ranging in complexity from simple bacterial isolates to higher eukaryotes such as plants and humans and even to communities of microbial organisms. Improvements to instrument performance, sample preparation, and informatic tools are increasing the scope and volume of data that can be analyzed by mass spectrometry (MS). To accommodate for these advances, it is becoming increasingly essential to choose and/or create tools that can not only scale well but also those that make more informed decisions using additional features within the data. Incorporating novel and existing tools into …
Chromatin Insulators: Master Regulators Of The Eukaryotic Genome, Todd Andrew Schoborg
Chromatin Insulators: Master Regulators Of The Eukaryotic Genome, Todd Andrew Schoborg
Doctoral Dissertations
Proper organization of the chromatin fiber within the three dimensional space of the eukaryotic nucleus relies on a number of DNA elements and their interacting proteins whose structural and functional consequences exert significant influence on genome behavior. Chromatin insulators are one such example, where it is thought that these elements assist in the formation of higher order chromatin loop structures by mediating long-range contacts between distant sites scattered throughout the genome. Such looping serves a dual role, helping to satisfy both the physical constraints needed to package the linear DNA polymer within the small volume of the nucleus while simultaneously …
A Mathematical Model And Numerical Method For Thermoelectric Dna Sequencing, Liwei Shi
A Mathematical Model And Numerical Method For Thermoelectric Dna Sequencing, Liwei Shi
Doctoral Dissertations
DNA sequencing is the process of determining the precise order of nucleotide bases, adenine, guanine, cytosine, and thymine within a DNA molecule. It includes any method or technology that is used to determine the order of the four bases in a strand of DNA. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and discovery. Thermoelectric DNA sequencing is a novel method to sequence DNA by measuring the heat that is released when DNA polymerase inserts a deoxyribonucleoside triphosphate into a growing DNA strand. The thermoelectric device for this project is composed of four parts: …
Experimental And Computational Analysis Of Chloroplast Transit Peptide Domain Architecture And Function, Prakitchai Chotewutmontri
Experimental And Computational Analysis Of Chloroplast Transit Peptide Domain Architecture And Function, Prakitchai Chotewutmontri
Doctoral Dissertations
The Majority of chloroplast proteins are nuclear-encoded and utilize an N-terminal transit peptide (TP) to target into chloroplasts via the general import pathway. Bioinformatic and proteomic analyses provide thousands of predicted TPs, which show low sequence similarity. How the common chloroplast translocon components recognize these diverse TPs is not well understood. Previous results support either sequence- or physicochemical-specific recognitions. To further address this question, a reverse sequence approach was utilized such that the reverse TP contains the same amino acid composition as wild-type TP but lack similar sequence motifs. Using both native and reverse TPs of the two well-studied precursors, …