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Effects Of Substrate And Temperature On Growth Of Aspergillus Flavus In Peanuts From Georgia, Premila Achar, Andres Sachez
Effects Of Substrate And Temperature On Growth Of Aspergillus Flavus In Peanuts From Georgia, Premila Achar, Andres Sachez
Faculty and Research Publications
We investigated the effects of different substrates (potato dextrose agar [PDA], nutrient agar [NA], and corn meal agar [CMA]) and temperatures on growth and aflatoxin production in Aspergillus flavus. Contaminated peanuts from retailers at different locations in Georgia were used throughout the experiment and 200 seeds were selected from each sample. PDA, NA, and CMA served as the substrate to express the mold from the contaminated seeds. Seeds were plated equidistantly on each of the media and incubated at 10° C, 27° C, 30° C, and 37° C, respectively. Seeds incubated on moist filter paper served as control. Neither growth …
Disease Resistance Conferred By The Expression Of A Gene Encoding A Synthetic Peptide In Transgenic Cotton (Gossypium Hirsutum L.) Plants, Kanniah Rajasekaran, Jeffrey W. Cary, Jesse M. Jaynes, Thomas E. Cleveland
Disease Resistance Conferred By The Expression Of A Gene Encoding A Synthetic Peptide In Transgenic Cotton (Gossypium Hirsutum L.) Plants, Kanniah Rajasekaran, Jeffrey W. Cary, Jesse M. Jaynes, Thomas E. Cleveland
Faculty and Research Publications
Fertile, transgenic cotton plants expressing the synthetic antimicrobial peptide, D4E1, were produced through Agrobacterium-mediated transformation. PCR products and Southern blots confirmed integration of the D4E1 gene, while RT-PCR of cotton RNA confirmed the presence of D4E1 transcripts. In vitro assays with crude leaf protein extracts from T0 and T1 plants confirmed that D4E1 was expressed at sufficient levels to inhibit the growth of Fusarium verticillioides and Verticillium dahliae compared to extracts from negative control plants transformed with pBI-d35SΩ-uidA-nos (CGUS). Although in vitro assays did not show control of pre-germinated spores of Aspergillus flavus, bioassays with cotton …