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Veterinary Preventive Medicine, Epidemiology, and Public Health

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2014

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Evaluation Of Porcine Ipec-J2 Cell Line Growth Rate And Immune Response To E. Coli (0111:B4) Lps, Xiaofan Wang Dec 2014

Evaluation Of Porcine Ipec-J2 Cell Line Growth Rate And Immune Response To E. Coli (0111:B4) Lps, Xiaofan Wang

Graduate Theses and Dissertations

The objective of this study was to evaluate the intestinal porcine epithelial cell-jejunum 2 (IPEC-J2) cell line as a model to study the innate immune function of live pigs. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were evaluated using a hemocytometer and spectrophotometer for cell quantity measurements to determine growth rate and doubling time. Growth rates of IPEC-J2 cells in T-75 flasks and 96-well plates were 0.4016 and 0.2851 times of doubling/day respectively with a doubling time of 1.73 d and 2.43 d, respectively. Confluent IPEC-J2 monolayers were tested at five time intervals (0, 1, 2, …


Glucose Significantly Enhances Enterotoxigenic Escherichia Coli Adherence To Intestinal Epithelial Cells Through Its Effects On Heat-Labile Enterotoxin Production, Prageeth Wijemanne, Rodney A. Moxley Nov 2014

Glucose Significantly Enhances Enterotoxigenic Escherichia Coli Adherence To Intestinal Epithelial Cells Through Its Effects On Heat-Labile Enterotoxin Production, Prageeth Wijemanne, Rodney A. Moxley

School of Veterinary and Biomedical Sciences: Faculty Publications

The present study tested whether exposure of enterotoxigenic Escherichia coli (ETEC) to glucose at different concentrations in the media results in increased bacterial adherence to host cells through increased heat-labile enterotoxin (LT) production, thereby suggesting the effects are physiological. Porcine-origin ETEC strains grown in Casamino acid yeast extract medium containing different concentrations of glucose were washed and inoculated onto IPEC-J2 porcine intestinal epithelial cells to test for effects on adherence and host cell cAMP concentrations. Consistent with previous studies, all LT+ strains had higher ETEC adherence to IPEC-J2 cells than did LT2 strains. Adherence of the LT2 but not the …


Infection And Immunity In The Pacific White Shrimp, Litopenaeus Vannamei, John Dustin Loy Oct 2014

Infection And Immunity In The Pacific White Shrimp, Litopenaeus Vannamei, John Dustin Loy

John Loy

Infectious diseases are significant impediments to the growth and sustainability of commercial shrimp aquaculture. Endemic diseases such as that caused by the obligate intracellular parasite the Necrotizing Hepatopancreatitis Bacterium (NHPB), cause significant losses to shrimp producers thoughout the Americas. New and emerging viral diseases, such as that caused by Infectious myonecrosis virus (IMNV), have caused tremendous economic losses in farmed Pacific white shrimp (Litopenaeus vannamei), since it emerged in Brazil and subsequently spread across the globe to Indonesia. No methods exist to propagate and model these infections in vitro), as no cell culture lines are available for shrimp. Therefore, we …


Acceptance Of Simulated Oral Rabies Vaccine Baits By Urban Raccoons, John Hadidian, Suzanne R. Jenkins, David H. Johnston, Peter J. Savarie, Victor F. Nettles, David M. Manski, George M. Baer Aug 2014

Acceptance Of Simulated Oral Rabies Vaccine Baits By Urban Raccoons, John Hadidian, Suzanne R. Jenkins, David H. Johnston, Peter J. Savarie, Victor F. Nettles, David M. Manski, George M. Baer

John Hadidian, PhD

In summer 1986, a study was conducted to evaluate raccoon (Procyon lotor) acceptance of oral baits that could be used for rabies vaccination, One thousand wax-coated sponge bait cubes were filled with 5 mg of a seromarker (iophenoxic acid), placed in polyethylene bags, and hand-distributed in an 80 ha area within an urban National Park in Washington, D.C. (USA), After 3 wk, target and nontarget animals were trapped and blood samples collected to evaluate bait uptake. Thirty-three of 52 (63%) raccoons had elevated blood iodine levels indicating they had eaten at least one bait, 13 (25%) were negative, and six …


The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger May 2014

The Human/Animal Interface: Emergence And Resurgence Of Zoonotic Infectious Diseases, Michael Greger

Michael Greger, MD, FACLM

Emerging infectious diseases, most of which are considered zoonotic in origin, continue to exact a significant toll on society. The origins of major human infectious diseases are reviewed and the factors underlying disease emergence explored. Anthropogenic changes, largely in land use and agriculture, are implicated in the apparent increased frequency of emergence and reemergence of zoonoses in recent decades. Special emphasis is placed on the pathogen with likely the greatest zoonotic potential, influenzavirus A.


Amyloid Fibrils: Potential Food Safety Implications, Michael Greger May 2014

Amyloid Fibrils: Potential Food Safety Implications, Michael Greger

Michael Greger, MD, FACLM

The demonstration of oral Amyloid-A (AA) fibril transmissibility has raised food safety questions about the consumption of amyloidotic viscera. In a presumed prion-like mechanism, amyloid fibrils have been shown to trigger and accelerate the development of AA amyloidosis in rodent models. The finding of amyloid fibrils in edible avian and mammalian food animal tissues, combined with the inability of cooking temperatures to eliminate their amyloidogenic potential, has led to concerns that products such as pâté de foie gras may activate a reactive systemic amyloidosis in susceptible consumers. Given the ability of amyloid fibrils to cross-seed the formation of chemically heterologous …


Their Bugs Are Worse Than Their Bite: Emerging Infectious Disease And The Human-Animal Interface, Michael Greger May 2014

Their Bugs Are Worse Than Their Bite: Emerging Infectious Disease And The Human-Animal Interface, Michael Greger

Michael Greger, MD, FACLM

In the twenty-five years since that announcement, what we now know as AIDS has killed 20 million people (National AIDS Trust 2005). Where did the AIDS virus— and other emerging diseases, such as severe acute respiratory syndrome (SARS), Ebola, mad cow— come from?


Effects On Pinniped Immune Response Upon In Vitro Exposure To The Perfluorinated Compounds, Pfos And Pfoa, Elizabeth J. Meiman May 2014

Effects On Pinniped Immune Response Upon In Vitro Exposure To The Perfluorinated Compounds, Pfos And Pfoa, Elizabeth J. Meiman

Honors Scholar Theses

This study investigated the effects of environmental toxicants on the immune system of two pinniped species, grey seals (Halichoerus grypus) and hooded seals (Cystophora cristata). The toxicants included two perfluorinated compounds (PFC), perfluorooctanoic acid (PFOA) and perfluorooctanesulfonic acid (PFOS), compounds commonly found in a wide variety of household consumer products, including Scotchguard and Teflon. Although corporations such as 3M curtailed the use of these chemicals in the past decade, concentrations of these chemicals are increasing in the arctic aquatic ecosystem and have been measured in the tissues and blood of arctic pinnipeds. However, the effects of …


The Spread And Potential Control Of Disease Across The Domestic Cattle-Wildlife Interface, Rachel Jackson May 2014

The Spread And Potential Control Of Disease Across The Domestic Cattle-Wildlife Interface, Rachel Jackson

Chancellor’s Honors Program Projects

No abstract provided.


Approaches To Canine Health Surveillance, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt Apr 2014

Approaches To Canine Health Surveillance, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt

Epidemiology Collection

Effective canine health surveillance systems can be used to monitor disease in the general population, prioritise disorders for strategic control and focus clinical research, and to evaluate the success of these measures. The key attributes for optimal data collection systems that support canine disease surveillance are representativeness of the general population, validity of disorder data and sustainability. Limitations in these areas present as selection bias, misclassification bias and discontinuation of the system respectively. Canine health data sources are reviewed to identify their strengths and weaknesses for supporting effective canine health surveillance. Insurance data benefit from large and well-defined denominator populations …


Forecasting The Spread Of Raccoon Rabies Using A Purpose-Specific Group Decision-Making Process., Aaron M. Anderson, Stephanie A. Shwiff, Richard B. Chipman, Todd C. Atwood, Tyler Cozzens, Frank Fillo, Robert Hale, Brody Hatch, Joanne Maki, Olin E. Rhodes Jr., Erin E. Rees, Charles E. Rupprecht, Rowland Tinline, Kurt C. Vercauteren, Dennis Slate Apr 2014

Forecasting The Spread Of Raccoon Rabies Using A Purpose-Specific Group Decision-Making Process., Aaron M. Anderson, Stephanie A. Shwiff, Richard B. Chipman, Todd C. Atwood, Tyler Cozzens, Frank Fillo, Robert Hale, Brody Hatch, Joanne Maki, Olin E. Rhodes Jr., Erin E. Rees, Charles E. Rupprecht, Rowland Tinline, Kurt C. Vercauteren, Dennis Slate

USDA Wildlife Services: Staff Publications

The Centers for Disease Control (CDC) and USDA Wildlife Services (WS) have been involved in an oral rabies vaccination (ORV) program for raccoons (Procyon lotor) that has slowed the westward spread of raccoon rabies. The objective of this study was to forecast the spread of the disease if an ORV zone was not maintained. A group decision-making process was designed to address the forecasting problem and was implemented using a group of 15 experts and 4 support personnel at a meeting at the USDA National Wildlife Research Center. Ten expansion regions were constructed that described the spread of …


Evaluation Of Wing Fractures On Survival And Re-Release, Katherine O'Neill Mar 2014

Evaluation Of Wing Fractures On Survival And Re-Release, Katherine O'Neill

Animal Science

No abstract provided.


Prevalence Of Disorders Recorded In Dogs Attending Primary-Care Veterinary Practices In England, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt Mar 2014

Prevalence Of Disorders Recorded In Dogs Attending Primary-Care Veterinary Practices In England, Dan G. O'Neill, David B. Church, Paul D. Mcgreevy, Peter C. Thomson, Dave C. Brodbelt

Epidemiology Collection

Purebred dog health is thought to be compromised by an increasing occurence of inherited diseases but inadequate prevalence data on common disorders have hampered efforts to prioritise health reforms. Analysis of primary veterinary practice clinical data has been proposed for reliable estimation of disorder prevalence in dogs. Electronic patient record (EPR) data were collected on 148,741 dogs attending 93 clinics across central and south-eastern England. Analysis in detail of a random sample of EPRs relating to 3,884 dogs from 89 clinics identified the most frequently recorded disorders as otitis externa (prevalence 10.2%, 95% CI: 9.1–11.3), periodontal disease (9.3%, 95% CI: …


Rabia Humana En Colombia En El Periodo 2005 - 2012, Factores Asociados A Su Presentación Y Recomendaciones Para Su Prevención, Sara Lorena Rojas Durán, Viviana Carolina Trujillo Rojas Jan 2014

Rabia Humana En Colombia En El Periodo 2005 - 2012, Factores Asociados A Su Presentación Y Recomendaciones Para Su Prevención, Sara Lorena Rojas Durán, Viviana Carolina Trujillo Rojas

Medicina Veterinaria

La rabia es una enfermedad zoonótica fatal y un problema de suma importancia para la salud pública tanto en Colombia como en el mundo entero. Todos los mamíferos son susceptibles de padecer la enfermedad y se sabe que en Colombia el virus ha circulado por todas las regiones. El objetivo de este estudio fue identificar factores asociados a la presentación de la enfermedad, así como algunas estrategias que sirvan para disminuir la presentación de casos. Para este fin se revisaron todas las historias clínicas y/o fichas de notificación (591) disponibles en el Instituto Nacional de Salud (INS). Esta información se …


Percepción De La Población Del Municipio De Guamal, Meta Sobre La Promoción Y Aplicación De Los Programas Antirrábicos En El Año 2014, Zully Esperanza Castañeda Suárez, Gina Jaqueline Ramírez Soto Jan 2014

Percepción De La Población Del Municipio De Guamal, Meta Sobre La Promoción Y Aplicación De Los Programas Antirrábicos En El Año 2014, Zully Esperanza Castañeda Suárez, Gina Jaqueline Ramírez Soto

Medicina Veterinaria

La rabia ha sido una enfermedad zoonótica muy temida, debido a su gran impacto en salud pública a nivel mundial. La vacuna antirrábica, es un tratamiento preventivo, eficaz e inocuo, pese a ser inmunoprevenible, muchas personas y animales enferman; alcanzando el porcentaje de letalidad hasta un 100% de los individuos que enferman. Anualmente mueren cerca de 50000 personas debido a este mal. Existen dos ciclos de la rabia, el ciclo urbano y el ciclo silvestre, en el primero, el animal transmisor es el perro; se estima que el 90% de los casos de rabia anual son debidos a este ciclo. …


Evaluación De La Efectividad De La Vacuna Escherichia Coli K99 En Bovinos De Leche En Dos Fincas En La Sabana De Bogotá, María Fernanda Soriano Toloza Jan 2014

Evaluación De La Efectividad De La Vacuna Escherichia Coli K99 En Bovinos De Leche En Dos Fincas En La Sabana De Bogotá, María Fernanda Soriano Toloza

Medicina Veterinaria

Se determino la efectividad de una vacuna Escherichia coli K99 en bovinos de leche en 2 fincas en la Sabana de Bogotá, en vacas en el ultimo tercio de la gestación y sus crías para observar el efecto que tiene la vacuna Escherichia coli K99 en la tasas de morbilidad, mortalidad ganancia de peso y alzada de los terneros hijos de las madres vacunadas, la evaluación de anticuerpos vacúnales y calostrales post-parto en las vacas contra la vacuna Escherichia coli K99 para así determinar la respuesta inmune y de anticuerpos vacúnales para así determinar la respuesta inmune del ternero usando …


Plan Estratégico Para El Control De Roedores Y Palomas En La Corporación De Abastos De Bogotá S.A, Johana Carolina Tafur Orozco, Jesús David Montes Acevedo Jan 2014

Plan Estratégico Para El Control De Roedores Y Palomas En La Corporación De Abastos De Bogotá S.A, Johana Carolina Tafur Orozco, Jesús David Montes Acevedo

Medicina Veterinaria

No abstract provided.


Effects Of Dietary Change On Viral-Bacterial Interactions In The Rumen Of Cattle, Christopher L. Anderson, Melissa L. Jolly, Adam L. Shreck Shreck, Galen E. Erickson, Terry J. Klopfenstein, Samodha C. Fernando Jan 2014

Effects Of Dietary Change On Viral-Bacterial Interactions In The Rumen Of Cattle, Christopher L. Anderson, Melissa L. Jolly, Adam L. Shreck Shreck, Galen E. Erickson, Terry J. Klopfenstein, Samodha C. Fernando

Nebraska Beef Cattle Reports

This ongoing study investigates the impact of diet and bacteriophage activity on the structuring of rumen microbial community composition and diversity. Fistulated cattle were acclimated to a given diet for 21 days before samples were collected and subsequently enriched for viral particles with tangential flow filtration. Taxonomic identification, abundance, and functional attributes were assigned to both bacterial and viral communities. Principle coordinate analysis of the bacterial communities revealed significant clustering based on diet. While diet drives the structuring of rumen bacterial communities, bacteriophages may maintain high, constant bacterial diversity.


Vein Steak Differences In Strip Loins Of Heifers Due To Mutation Of The Myostatin Gene, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson Jan 2014

Vein Steak Differences In Strip Loins Of Heifers Due To Mutation Of The Myostatin Gene, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson

Nebraska Beef Cattle Reports

Strip loins from heifers with variations of the myostatin gene; 19 homozygous dominant (Angus), 20 heterozygous dominant (Angus x Piedmontese), and 20 homozygous recessive (Piedmontese) were studied. Strip loins were cut into 1-inch thick steaks and total number of steaks and number of steaks with Gluteusmedius (often called vein steaks) were recorded. Strip loins from heterozygous dominant heifershad a greater number of non-vein steaks and decreased percentage of vein steaks compared to homozygous dominant and homozygous recessive samples. Differences in percentage of vein steaks were inconsistent and showed no meaningful pattern.


Energy Value Of De-Oiled Modified Distillers Grains Plus Solubles In A Forage-Based Diet, Meredith L. Bremer, Andrea K. Watson, Dirk B. Burken Burken, James C. Macdonald, Galen E. Erickson Jan 2014

Energy Value Of De-Oiled Modified Distillers Grains Plus Solubles In A Forage-Based Diet, Meredith L. Bremer, Andrea K. Watson, Dirk B. Burken Burken, James C. Macdonald, Galen E. Erickson

Nebraska Beef Cattle Reports

Sixty individually fed steers were used to determine the effects of feeding de-oiled modified distillers grains plus solubles (MDGS) on steer performance in an 84-day forage-based growing study. De-oiled MDGS did not significantly alter performance when compared to normal MDGS if fed at the same concentration in growing diets. Inclusion of either de-oiled or normal MDGS at 40% of the diet resulted in improved ending BW, DMI, ADG, and F:G as compared to inclusion of 20% MDGS in the diet.


Transitioning Cattle From Ramp® To A Finishing Diet On Feedlot Performance And Feed Intake Variance, Cody J. Schneider, Brandon L. Nuttelman, Dirk B. Burken Burken, Terry J. Klopfenstein, Galen E. Erickson Jan 2014

Transitioning Cattle From Ramp® To A Finishing Diet On Feedlot Performance And Feed Intake Variance, Cody J. Schneider, Brandon L. Nuttelman, Dirk B. Burken Burken, Terry J. Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Individually fed cattle were adapted to high grain diets with a traditional grain adaptation program or one of two RAMP® adaptation programs. RAMP programs adapted cattle to a finishing diet gradually over 28 days in four steps or directly without an adaptation. Feed intake variance among d was greater for traditionally adapted cattle compared to either RAMP program, but DMI was not different during the adaptation period. Over the 138-day period, feedlot performance and carcass traits were not affected by adaptation treatment. Cattle fed RAMP for 10 days can be transitioned to a finishing ration containing 47.5% Sweet Bran …


Using Enspira™ To Improve Fiber Digestion, Jana L. Harding, Galen E. Erickson, James C. Macdonald Donald Jan 2014

Using Enspira™ To Improve Fiber Digestion, Jana L. Harding, Galen E. Erickson, James C. Macdonald Donald

Nebraska Beef Cattle Reports

Two experiments evaluated the effectof treating various feedstuffs with an enzyme(Enspira) on digestibility. Twelve feeds commonly fed to beef cattle were treated with four levels of the enzyme(0, 0.25, 0.50, or 0.75 lb of enzyme per ton of DM). Enzyme treatment increased in vitro DMD of high moisture corn (HMC), wet distillers grains plus solubles (WDGS), corn bran, and husks. There was a quadratic increasein gas production for corn leaves, as well as a linear increase in gas production for corn bran treated with increasing levels of Enspira. Treating feeds with the commercial enzyme Enspiraimproved in vitro digestibility of feeds …


Nutrient And Tenderness Differences Of Beef From Heifers Due To Mutation Of The Myostatin Genehypothesized, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson Jan 2014

Nutrient And Tenderness Differences Of Beef From Heifers Due To Mutation Of The Myostatin Genehypothesized, Michelle E. Semler, Chris R. Calkins, Galen E. Erickson Erickson

Nebraska Beef Cattle Reports

Strip loins and eye of rounds were obtained from heifers genotyped with variations of the myostatin gene; 19 homozygous dominant (Angus), 20 heterozygous dominant (Angus x Piedmontese), and 20 homozygous recessive (Piedmontese). Steaks were aged for 14 days, cooked fresh (never frozen), and nutrient steaks were frozen three days postmortem. Meat from homozygous recessive heifers was equal in tenderness to homozygous dominant and heterozygous dominant heifers. Fat content of meat from homozygous recessive heifers decreased while moisture and protein increased compared to homozygous dominant and heterozygous dominant. Calorie content decreased with increasing copies of the recessive gene. Thus, meat from …


Effect Of Feeding De-Oiled Wet Distillers Grains Plus Solubles On Beef Oxidation And Tenderness, Katherine I. Domenech, Kim A. Varnold, Michelle E. Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson, Chris R. Calkins Jan 2014

Effect Of Feeding De-Oiled Wet Distillers Grains Plus Solubles On Beef Oxidation And Tenderness, Katherine I. Domenech, Kim A. Varnold, Michelle E. Semler, Michael D. Chao, Tommi F. Jones, Galen E. Erickson, Chris R. Calkins

Nebraska Beef Cattle Reports

Cattle fed a de-oiled wet distiller’s grains plus solubles (WDGS) diet were compared to cattle fed corn or traditional (full-fat) WDGS diets to determine effects on discoloration, oxidation, and tenderness of beef aged for seven and 21 days. At seven days of aging, dietary treatment had no effect on discoloration. At 21 days of aging, beef from cattle fed de-oiled WDGS had less oxidation than the corn control and several of the full-fat WDGS treatments. Although tenderness increased with aging and retail display, dietary treatment had no effect on tenderness. These findings suggest that these dietary treatments, followed by a …


Effects Of Feeding Distillers Grains In A Yearling Beef System On Meat Quality, Kelby M. Sudbeck, Kimberly A. Varnold, James C. Macdonald Donald, Chris R. Calkins, Galen E. Erickson Jan 2014

Effects Of Feeding Distillers Grains In A Yearling Beef System On Meat Quality, Kelby M. Sudbeck, Kimberly A. Varnold, James C. Macdonald Donald, Chris R. Calkins, Galen E. Erickson

Nebraska Beef Cattle Reports

Distillers grains use while wintering on cornstalks during summer grazing and during the finishing period was evaluated to determine the effects of lifetime exposure to distillers grains on meat characteristics. Finishing diets with distillers grains increased discoloration in steaks following six days of retail display for steaks aged seven days, and after four days of retail display for steaks aged 21 days. Supplementation during summer grazing increased discoloration when cattle were not finished using distillers grains. There were no differences in oxidative rancidity among dietary treatments. Supplementing with distillers grains prior to finishing was not additive in impacting the color …


Differences In Fecal Bacterial Community Composition Between Beef Steers Which Are High-Shedders And Low-Shedders Of Shiga Toxin-Producing Escherichia Coli (Stec), Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando Jan 2014

Differences In Fecal Bacterial Community Composition Between Beef Steers Which Are High-Shedders And Low-Shedders Of Shiga Toxin-Producing Escherichia Coli (Stec), Nirosh D. Aluthge, Yoshitha A. Wanniarachchi, Brandon L. Nuttelman Nuttelman, Cody J. Schneider, Terry J. Klopfenstein, Galen E. Erickson, Samodha C. Fernando

Nebraska Beef Cattle Reports

The community composition of the fecal microbiota was compared between beef steers which were high-shedders and low-shedders of Shiga toxin-producing Escherichia coli. Based on Shannon and Chao 1 diversity indices, the high-shedders had a more diverse fecal bacterial community than the low-shedding steers. Members of the genus Prevotella were observed as being more abundant in the low-shedders compared to the high-shedders, while Succinivibrio were more abundant in the high-shedders. Isolation of specific bacteria which are significantly more abundant in low-shedders may pave the way to developing direct-fed microbials which are effective in reducing STEC shedding among high-shedding beef steers.


Follicular Vascular Endothelial Growth Factor A Expression Before And After The Lh Surge, Renee M. Mcfee, Robin A. Artac, Adam F. Summers, William E. Pohlmeier, Vanessa M. Brauer, Scott G. Kurz, Robert A. Cushman, Jennifer R. Wood, Andrea S. Cupp Jan 2014

Follicular Vascular Endothelial Growth Factor A Expression Before And After The Lh Surge, Renee M. Mcfee, Robin A. Artac, Adam F. Summers, William E. Pohlmeier, Vanessa M. Brauer, Scott G. Kurz, Robert A. Cushman, Jennifer R. Wood, Andrea S. Cupp

Nebraska Beef Cattle Reports

Granulosa cell expression of VEGFAisoforms in dominant bovine follicles was evaluated. Collection of granulosa cells via follicle aspiration revealed altered expression of the proangiogenic VEGFA_164 isoform but not the antiangiogenic VEGFA_164B isoform prior to and after the LH surge. Expression of VEGFA_164 declines as both the LH surge and ovulation approaches. In addition, VEGFA_164 and VEGFA_164B expression prior to the LH surge was positively correlated with FSHR and CYP19A1 expression, suggesting that VEGFA expression may be regulated by FSH. These data indicate differential expression of VEGFA isoforms may be an important feature of bovine dominant follicle development.


Androgen Excess In Beef Cows Results In Altered Theca Cell Gene Expression And Fertilityand, Adam F. Summers, William E. Pohlmeier, Vanessa M. Brauer Brauer, Kevin M. Sargent, Renee M. Mcfee, Scott G. Kurz, Robert A. Cushman, Jennifer R. Wood, Andrea S. Cupp Jan 2014

Androgen Excess In Beef Cows Results In Altered Theca Cell Gene Expression And Fertilityand, Adam F. Summers, William E. Pohlmeier, Vanessa M. Brauer Brauer, Kevin M. Sargent, Renee M. Mcfee, Scott G. Kurz, Robert A. Cushman, Jennifer R. Wood, Andrea S. Cupp

Nebraska Beef Cattle Reports

Within the University of Nebraska–Lincoln physiology herd, two sub-populations of cows with different concentrations of androstenedione have been identified. Androstenedione is a precursor for estradiol production, and androstenedione concentration is increased 24.5-fold in the high androstenedione cows. Our objective was to determine the cause of increased androstenedione production in high androstenedione cows and the effects on theca cell and oocyte gene expression. High androstenedione cows had increased steroidogenic enzyme abundance in theca cells and altered oocyte mRNA abundance. Increased androgen production in high androstenedione cows is associated with altered gene expression and/or mRNA stability during oocyte growth and maturation, which …


Effect Of Pubertal Status And Number Of Estrous Cycles Prior To The Breeding Season On Pregnancy Rate In Beef Heifers, Rebecca A. Vraspir, Adam F. Summers, Andrew J. Roberts Roberts, Rick N. Funston Jan 2014

Effect Of Pubertal Status And Number Of Estrous Cycles Prior To The Breeding Season On Pregnancy Rate In Beef Heifers, Rebecca A. Vraspir, Adam F. Summers, Andrew J. Roberts Roberts, Rick N. Funston

Nebraska Beef Cattle Reports

Three experiments were conducted to evaluate whether pubertal status and number of estrous cycles prior to breeding influences pregnancy rate in beef heifers. Pubertal heifers were heavier and older at the start of breeding and had greater AI and overall pregnancy rate than non-pubertal heifers. Second season pregnancy rate was greater for heifers reaching puberty prior to first breeding and for heifers having ≥ 2 estrouscycles prior to breeding compared with non-pubertal heifers. Pregnancy rate was greater for heifers achieving puberty prior to breeding; however, earlier onset of puberty did not significantly improve first pregnancy rates.


Economics Of Distillers Grains Supplementation In A Forage System With Spayed Heifers, Kari L. Gillespie, Terry J. Klopfenstein, James C. Macdonald Donald, Brandon L. Nuttelman, Cody J. Schneider, Galen Erickson, J. D. Volesky Jan 2014

Economics Of Distillers Grains Supplementation In A Forage System With Spayed Heifers, Kari L. Gillespie, Terry J. Klopfenstein, James C. Macdonald Donald, Brandon L. Nuttelman, Cody J. Schneider, Galen Erickson, J. D. Volesky

Nebraska Beef Cattle Reports

In a two-year study, spayed heifer calves were backgrounded on cornstalks with 2 lb or 5 lb wet distillers grains with solubles supplemented daily. During the summer, heifers grazed native range and received no summer supplementation or were supplemented with modified distillers grains with solubles at 0.6% BW daily. Heifers were finished on a common regimen, and an economic scenario was applied to each phase of production and overall. Supplementing more in winter increased profit, but summer supplementation did not impact overall profitability. Numerically, heifers not supplemented during the summer were more profitable than supplemented heifers.