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Geographical And Temporal Distribution Of Human Giardiasis In Ontario, Canada., Agricola Odoi, S W. Martin, P Michel, J Holt, D Middleton, J Wilson

Faculty Publications and Other Works -- Biomedical and Diagnostic Sciences

BACKGROUND: Giardia is the most frequently identified intestinal parasite in North America. Although information on geographical distribution of giardiasis is critical in identifying communities at high risk, little has been done in this area. Therefore, the objective of this study was to investigate the geographical and temporal distribution of human giardiasis in Ontario in order to identify possible high risk areas and seasons. Two spatial scales of analyses and two disease measures were used with a view to identifying the best of each in assessing geographical patterns of giardiasis in Ontario. Global Moran's I and Moran Local Indicators of Spatial …

Expression And Localization Of Estrogen Receptor-Alpha Protein In Normal And Abnormal Term Placentae And Stimulation Of Trophoblast Differentiation By Estradiol, A Bukovsky, Maria Cekanova Ms, Rndr, Phd, Mr Caudle, J Wimalasena, Js Foster, Dc Henley, Rf Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Estrogens play an important role in the regulation of placental function, and 17-beta-estradiol (E2) production rises eighty fold during human pregnancy. Although term placenta has been found to specifically bind estrogens, cellular localization of estrogen receptor alpha (ER-alpha) in trophoblast remains unclear. We used western blot analysis and immunohistochemistry with h-151 and ID5 monoclonal antibodies to determine the expression and cellular localization of ER-alpha protein in human placentae and cultured trophoblast cells. Western blot analysis revealed a ~65 kDa ER-alpha band in MCF-7 breast carcinoma cells (positive control). A similar band was detected in five normal term placentae exhibiting strong …

Comparison Of Phenotypic Traits And Genetic Relatedness Of Salmonella Enterica Subspecies Arizonae Isolates From A Colony Of Ridgenose Rattlesnakes With Osteomyelitis, Davis A. Bemis, M A. Owston, A La Lickey, Stephen A. Kania, P Ebner, Barton W. Rohrbach Dr., Edward C. Ramsay

Faculty Publications and Other Works -- Biomedical and Diagnostic Sciences

Reptiles are well-known sources of human Salmonella infections; however, little is known about the ability of Salmonella to cause disease in reptiles. Thirty-seven isolates of Salmonella enterica subspecies arizonae (S. arizonae) were obtained from retrospective and prospective studies of a closed colony of ridgenose rattlesnakes (Crotalus willardi) with osteomyelitis. All isolates (n=7) from bone lesions were of a single serotype, 56:z4,z23, and this serotype was found on only one occasion among 8 other serotypes isolated from 21 cloacal and intestinal samples. The remainder (n=7) of serotype 56:z4,z23 isolates were from other extraintestinal sites, including liver, ovary, blood, and testis. S. …

Placental Expression Of Estrogen Receptor Beta And Its Hormone Binding Variant – Comparison With Estrogen Receptor Alpha And A Role For Estrogen Receptors In Asymmetric Division And Differentiation Of Estrogen-Dependent Cells, Antonin Bukovsky, Michael R. Caudle, Maria Cekanova Ms, Rndr, Phd, Romaine I. Fernando, Jay Wimalasena, James S. Foster, Donald C. Henley, Robert F. Elder

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

During human pregnancy, the production of 17-beta-estradiol (E2) rises steadily to eighty fold at term, and placenta has been found to specifically bind estrogens. We have recently demonstrated the expression of estrogen receptor alpha (ER-alpha) protein in human placenta and its localization in villous cytotrophoblast (CT), vascular pericytes, and amniotic fibroblasts. In vitro, E2 stimulated development of large syncytiotrophoblast (ST) aggregates. In the present study we utilized ER-beta affinity purified polyclonal (N19:sc6820) and ER-alpha monoclonal (clone h-151) antibodies. Western blot analysis revealed a single ~52 kDa ER-beta band in chorionic villi (CV) protein extracts. In CV, strong cytoplasmic ER-beta immunoreactivity …

Volume 1, Number 2 (2003), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Insects as Teaching Tools
• Helping Children Succeed
• Honors Student Faces Bright Future
• Reinventing the Family Farm

Volume 1, Number 3 (2003), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Booming Biotechnology
• Lessons in Leadership
• New Options for Producers
• Entering an Exciting New Era

Volume 1, Number 1 (2003), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Adding Value to Dogwoods
• A Building to Advance Science
• Teaching Lessons in Life
• Towards a Brighter Forest Future