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Social and Behavioral Sciences

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2009

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Development Of Non-Antibiotic-Resistant, Chromosomally Based, Constitutive And Inducible Expression Systems For Aroa-Attenuated Salmonella Enterica Serovar Typhimurium, Jake Matic, Tamsin D. Terry, David Van Bockel, Tracy A. Maddocks, David Tinworth, Michael Jennings, Steven P Djordjevic, Mark J. Walker Jan 2009

Development Of Non-Antibiotic-Resistant, Chromosomally Based, Constitutive And Inducible Expression Systems For Aroa-Attenuated Salmonella Enterica Serovar Typhimurium, Jake Matic, Tamsin D. Terry, David Van Bockel, Tracy A. Maddocks, David Tinworth, Michael Jennings, Steven P Djordjevic, Mark J. Walker

Faculty of Science - Papers (Archive)

Live-vaccine delivery systems expressing two model antigens from Mycoplasma hyopneumoniae, F2(P97) (Adh) and NrdF, were constructed using Salmonella enterica serovar Typhimurium aroA (STM-1), and immunogenicity in mice was evaluated. Recombinant plasmid-based expression (PBE) and chromosomally based expression (CBE) systems were constructed. The PBE system was formed by cloning both antigen genes into pJLA507 to create an operon downstream of temperature-inducible promoters. Constitutive CBE was achieved using a promoter-trapping technique whereby the promoterless operon was stably integrated into the chromosome of STM-1, and the expression of antigens was assessed. The chromosomal position of the operon was mapped in four clones. Inducible …


Gremo: A Gis-Based Generic Model For Estimating Relative Wave Exposure, Austen Pepper, Marjetta L. Puotinen Jan 2009

Gremo: A Gis-Based Generic Model For Estimating Relative Wave Exposure, Austen Pepper, Marjetta L. Puotinen

Faculty of Science - Papers (Archive)

Wave exposure plays a major role in shaping the ecological structure of nearshore communities, with different community types able to survive and/or thrive when typically exposed to different levels of wave energy. This can be quantified by taking direct field measurements with wave buoys over time and then manipulating the data to derive typical conditions. However, taking these measurements is only feasible for very limited areas due to logistical constraints, and generating them with numerical wave models can also be expensive to run and may require data inputs that are either lacking or are highly uncertain. Instead, the relative differences …


Usability Testing Of Public Health Web-Based Information Systems, Sumayya Banna, Kholoud Alkayid, Helen M. Hasan, Joseph A. Meloche Jan 2009

Usability Testing Of Public Health Web-Based Information Systems, Sumayya Banna, Kholoud Alkayid, Helen M. Hasan, Joseph A. Meloche

Faculty of Commerce - Papers (Archive)

While the Internet provides many opportunities for increased levels of care and access to information services in the area of public health, many web designers are not yet taking full advantage of its potential. This study looks at Intensive Care and Palliative Care, as important instances where health informatics could improve public web-based services, in meeting the particular information needs of family members of critically and chronically ill patients. This study is significant in adopting an approach to the usability testing of websites based on concepts from Activity Theory. This takes a realistic and practical approach, which identifies the purpose …