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Plant Sciences

2013

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Arabidopsis Thaliana Ahl Family Modulates Hypocotyl Growth Redundantly By Interacting With Each Other Via The Ppc/Duf296 Domain, Jianfei Zhao, David Favero, Hao Peng, Michael Neff Nov 2013

Arabidopsis Thaliana Ahl Family Modulates Hypocotyl Growth Redundantly By Interacting With Each Other Via The Ppc/Duf296 Domain, Jianfei Zhao, David Favero, Hao Peng, Michael Neff

Jianfei Zhao

The Arabidopsis thaliana genome encodes 29 AT-HOOK MOTIF CONTAINING NUCLEAR LOCALIZED (AHL) genes, which evolved into two phylogenic clades. The AHL proteins contain one or two AT-hook motif(s) and one plant and prokaryote conserved (PPC)/domain of unknown function #296 (DUF296) domain. Seedlings lacking both SOB3/AHL29 and ESC/AHL27 confer a subtle long-hypocotyl phenotype compared with the WT or either single-null mutant. In contrast, the missense allele sob3-6 confers a dramatic long-hypocotyl phenotype in the light. In this study, we examined the dominant-negative feature of sob3-6 and found that it encodes a protein with a disrupted AT-hook motif that abolishes binding to …


Long-Distance Translocation Of Photosynthates: A Primer [Review Article], Michael Knoblauch, Winfried S. Peters Oct 2013

Long-Distance Translocation Of Photosynthates: A Primer [Review Article], Michael Knoblauch, Winfried S. Peters

Winfried S. Peters

The storage of light energy in chemical form through photosynthesis is the key process underlying life as we know it. To utilize photosynthates efficiently as structural materials or as fuel to drive endergonic processes, they have to be transported from where they are produced to where they are needed. In this primer, we provide an overview of basic biophysical concepts underlying our current understanding of the mechanisms of photosynthate long-distance transport, and briefly discuss current developments in the field.


Phytochemical And Pharmacological Aspects Of Australian Bloodroots (Haemodora Spp.), Lei Liu, Myrna Deseo, D Thompson, Hans Wohlmuth, David Leach Oct 2013

Phytochemical And Pharmacological Aspects Of Australian Bloodroots (Haemodora Spp.), Lei Liu, Myrna Deseo, D Thompson, Hans Wohlmuth, David Leach

Dr Hans Wohlmuth

No abstract provided.


Phytochemistry And Pharmacology Of Volatile Components Of Plectranthus Graveolens R. Br., L Cyril, Myrna Deseo, G Buchbauer, P Forster, David Leach Oct 2013

Phytochemistry And Pharmacology Of Volatile Components Of Plectranthus Graveolens R. Br., L Cyril, Myrna Deseo, G Buchbauer, P Forster, David Leach

Dr Myrna A Deseo

No abstract provided.


Phytochemical And Pharmacological Aspects Of Australian Bloodroots (Haemodora Spp.), Lei Liu, Myrna Deseo, D Thompson, Hans Wohlmuth, David Leach Oct 2013

Phytochemical And Pharmacological Aspects Of Australian Bloodroots (Haemodora Spp.), Lei Liu, Myrna Deseo, D Thompson, Hans Wohlmuth, David Leach

Dr Myrna A Deseo

No abstract provided.


Compounds From Saccharum Officinarum L. As Leads For Anti-Diabetes Treatment, D Payne, David Leach, C Morris, M O'Shea, Lei Liu, Kellie Shepherd, Myrna Deseo Oct 2013

Compounds From Saccharum Officinarum L. As Leads For Anti-Diabetes Treatment, D Payne, David Leach, C Morris, M O'Shea, Lei Liu, Kellie Shepherd, Myrna Deseo

Dr Myrna A Deseo

No abstract provided.


Seed Biology Research And Conservation Of Eastern Australian Rainforest Diversity, K Hamilton, C Offord, P Cuneo, S Ashmore, Myrna Deseo Oct 2013

Seed Biology Research And Conservation Of Eastern Australian Rainforest Diversity, K Hamilton, C Offord, P Cuneo, S Ashmore, Myrna Deseo

Dr Myrna A Deseo

No abstract provided.


Gc Analysis Of Aqueous Plant Extracts, K Shepherd, V Westbrooke, Myrna Deseo Oct 2013

Gc Analysis Of Aqueous Plant Extracts, K Shepherd, V Westbrooke, Myrna Deseo

Dr Myrna A Deseo

No abstract provided.


Modulation Of Natural Killer Cell Activity By Water Soluble Polysaccharides From The Roots Of Asparagus Racemosus Linn, M Thakur, P Connellan, Myrna Deseo, Carol Morris, V Dixit Oct 2013

Modulation Of Natural Killer Cell Activity By Water Soluble Polysaccharides From The Roots Of Asparagus Racemosus Linn, M Thakur, P Connellan, Myrna Deseo, Carol Morris, V Dixit

Dr Myrna A Deseo

Asparagus racemosus L. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant [1]. The plant belongs to the category of 'Rasayana' i.e., plants possessing immunomodulatory and adaptogenic activity [2]. Although there have been claims on immune system potentiation by aqueous extracts of A. racemosus, there is a lack of information specifying the exact molecular mechanism and phytochemical components responsible for the immuno-stimulatory activity.

In the present study, an aqueous extract of A. racemosus (ARC) was further fractionated to isolate the polysaccharide fraction (ARP). The isolated polysaccharides (ARP) were characterized by enzymatic, size exclusion, GC-FID, and thin layer chromatographic analyses. …


Preliminary Investigation Into Milk Flavour, V Westbooke, H Smyth, K Shepherd, Carol Morris, B Granzin, Myrna Deseo Oct 2013

Preliminary Investigation Into Milk Flavour, V Westbooke, H Smyth, K Shepherd, Carol Morris, B Granzin, Myrna Deseo

Dr Myrna A Deseo

No abstract provided.


Preliminary Investigation Into The Phytochemical And Pharmacological Aspects Of Plectranthus Species, E Pasoski, L Marangattil, Myrna Deseo, David Leach, P Forster Oct 2013

Preliminary Investigation Into The Phytochemical And Pharmacological Aspects Of Plectranthus Species, E Pasoski, L Marangattil, Myrna Deseo, David Leach, P Forster

Dr Myrna A Deseo

No abstract provided.


Rainforest Seed Project, K Hamilton, C Offord, P Cuneo, S Ashmore, Myrna Deseo, K Shepherd Oct 2013

Rainforest Seed Project, K Hamilton, C Offord, P Cuneo, S Ashmore, Myrna Deseo, K Shepherd

Dr Myrna A Deseo

No abstract provided.


Preliminary Investigation Into The Phytochemical And Pharmacological Aspects Of Plecthrantus Sp. 'Hann Tableland', E Pasoski, Myrna Deseo, G Buchbauer, Lei Liu, P Forster, David Leach Oct 2013

Preliminary Investigation Into The Phytochemical And Pharmacological Aspects Of Plecthrantus Sp. 'Hann Tableland', E Pasoski, Myrna Deseo, G Buchbauer, Lei Liu, P Forster, David Leach

Dr Myrna A Deseo

No abstract provided.


Chemical Composition And Biological Activity Of The Essential Oils From Native Australian Callitris Species, Angela Oprava, David Leach, Karren Beattie, Paul Connellan, Paul Forster, Greg Leach, Gerhard Buchbauer, Kellie Shepherd, Myrna Deseo Oct 2013

Chemical Composition And Biological Activity Of The Essential Oils From Native Australian Callitris Species, Angela Oprava, David Leach, Karren Beattie, Paul Connellan, Paul Forster, Greg Leach, Gerhard Buchbauer, Kellie Shepherd, Myrna Deseo

Dr Myrna A Deseo

The chemical composition of the essential oil of the wood of the Australian native cypress Callitris endlicheri, C. intratropica and C. columellaris were determined by GCMS. The colour of the oils from C. endlicheri were yellow, yellow green and light green, whilst the distinct blue colour of the oil from C. intratropica was in keeping with its generic name ‘blue cypress oil'. The sesquiterpene alcohol guaiol (1) comprised >50% of the C. endlicheri oil. Significant differences were observed in components between the three accessions. In contrast, the oils of C. intratropica showed more subtle variations between collection …


Sugarcane Molasses – A Potential Source Of Anti-Diabetic Compounds, D Payne, R Taylor, Myrna Deseo, M O'Shea, N Masel, David Leach Oct 2013

Sugarcane Molasses – A Potential Source Of Anti-Diabetic Compounds, D Payne, R Taylor, Myrna Deseo, M O'Shea, N Masel, David Leach

Dr Myrna A Deseo

No abstract provided.


Chemical Variation In The Leaf Essential Oils Of Australian Corymbia Spp., Myrna Deseo, K Shepherd, M Abasolo, Mervyn Shepherd, D Lee Oct 2013

Chemical Variation In The Leaf Essential Oils Of Australian Corymbia Spp., Myrna Deseo, K Shepherd, M Abasolo, Mervyn Shepherd, D Lee

Dr Myrna A Deseo

No abstract provided.


Isolation And Identification Of Antioxidant Compounds From Carica Papaya L, E Jensen, A Larsen, David Leach, Myrna Deseo Oct 2013

Isolation And Identification Of Antioxidant Compounds From Carica Papaya L, E Jensen, A Larsen, David Leach, Myrna Deseo

Dr Myrna A Deseo

No abstract provided.


Conserving Australia's Unique Rainforest Fruits And Wild Relatives, Kim N Hamilton, Catherine A Offord, Peter Cuneo, Sarah E Ashmore, Myrna Deseo Oct 2013

Conserving Australia's Unique Rainforest Fruits And Wild Relatives, Kim N Hamilton, Catherine A Offord, Peter Cuneo, Sarah E Ashmore, Myrna Deseo

Dr Myrna A Deseo

Australian rainforests have many unique edible fruits including wild relatives of worldwide economically important species such as citrus and macadamia. One of the key risks of projected climate change is its effect on Eastern Australian rainforests. The interaction of climate change with other threats, such as weeds and habitat fragmentation, is thus likely to impact on already vulnerable environments such as the World Heritage listed Gondwana Rainforests of S. Queensland and N. New South Wales. Seed banking is a cost effective way of conserving unique and vulnerable diversity ex situ for utilisation (e.g., plant breeding and restoration programs). However, the …


Investigation Of A-Glucosidase Inhibitory Activity Of Wheat Bran And Germ, Lei Liu, Myrna Deseo, Carol Morris, Kelly Winter, David Leach Oct 2013

Investigation Of A-Glucosidase Inhibitory Activity Of Wheat Bran And Germ, Lei Liu, Myrna Deseo, Carol Morris, Kelly Winter, David Leach

Dr Myrna A Deseo

Postprandial hyperglycaemia is a primary risk factor in the development of Type 2 diabetes. α-Glucosidaseinhibitors that reduce postprandial hyperglycaemia have a key role in the treatment of Type 2 pre-diabetic states and also have the potential to reduce the progression to complications of diabetes. Epidemiological studies showed that risk for Type 2 diabetes mellitus was decreased with consumption of whole grains. Thebran and germ of whole wheat are major components of whole grain consumption and are widely accepted as important ingredients in many low glycaemic index (GI) foods. In this study, the α-glucosidaseinhibitoryactivity of wheatbran and germ was investigated. The …


Phytochemical Study Of Fagraea Spp. Uncovers A New Terpene Alkaloid With Anti-Inflammatory Properties1, Suciati, Lynette Lambert, Benjamin Ross, Myrna Deseo, Mary Garson Oct 2013

Phytochemical Study Of Fagraea Spp. Uncovers A New Terpene Alkaloid With Anti-Inflammatory Properties1, Suciati, Lynette Lambert, Benjamin Ross, Myrna Deseo, Mary Garson

Dr Myrna A Deseo

Phytochemical investigation of the stem bark of F. racemosa JACK ex WALL (Loganiaceae) from East Java, Indonesia, has resulted in the isolation of a new alkaloid fagraeoside along with the iridoid glycoside secologanoside. Fagraeoside may be derived from the condensation of secologanin with L-asparagine, and represents a rare example of a terpene alkaloid in which the amino acid component is non-aromatic. Investigation of three additional species ofFagraea provided known lignans, iridoid or secoiridoid glycosides, and flavanol-6-C-glucosides, thus it is likely that iridoid and secoiridoid glucosides are chemotaxonomic markers for the Fagraea genus. Fagraeoside inhibited the production of prostaglandin E2 in …


Characterization And In Vitro Immunomodulatory Screening Of Fructo-Oligosaccharides Of Asparagus Racemosus Willd, Mayank Thakur, Paul Connellan, Myrna Deseo, Carol Morris, Werner Praznik, Loeppert, Dixit Oct 2013

Characterization And In Vitro Immunomodulatory Screening Of Fructo-Oligosaccharides Of Asparagus Racemosus Willd, Mayank Thakur, Paul Connellan, Myrna Deseo, Carol Morris, Werner Praznik, Loeppert, Dixit

Dr Myrna A Deseo

Asparagus racemosus Linn. (Fam. Liliaceae) is an ethno-pharmacologically acclaimed Ayurvedic medicinal plant. In the present study, aqueous extract of A. racemosus (ARC) was fractionated and screened for the polysaccharide fraction (ARP). The characterization was done by enzymatic, Size Exclusion, gas chromatography with flame ionization detector (GC–FID), high pressure anion exchange chromatography (HPAEC) and thin layer chromatographic analyses. Phyto-chemical evaluation confirmed the presence of 26.7% of 2 → 1 linked fructo-oligosaccharides (FOS). They have a degree of polymerization (DP) of nearly 7–8. Cytotoxicity evaluation on P388 cell lines was consistent with low cytotoxicity of the extracts. In vitroNatural Killer (NK) cell …


Genomics And Metabolomics For New Plant Products, Daniel Waters, Peter Bundock, Hans Wohlmuth, Myrna Deseo, Lei Liu, Frances Shapter, Agnelo Furtado Oct 2013

Genomics And Metabolomics For New Plant Products, Daniel Waters, Peter Bundock, Hans Wohlmuth, Myrna Deseo, Lei Liu, Frances Shapter, Agnelo Furtado

Dr Myrna A Deseo

No abstract provided.


Novel Polyketide Metabolites From Streptomyces Rimosus Mutant Strain R1059, Myrna Deseo, Iain Hunter, Peter Waterman Oct 2013

Novel Polyketide Metabolites From Streptomyces Rimosus Mutant Strain R1059, Myrna Deseo, Iain Hunter, Peter Waterman

Dr Myrna A Deseo

Three novel polyketide metabolites were isolated from laboratory-scale fermentation of the Streptomyces rimosus mutant strain R1059. Structural elucidation of the compounds was based on NMR experiments. The compounds were characterized as naphthalene derivatives: (rel)-4,8-dihydroxy-3-hydroxymethyl-4-methyl-1,2,3,4-tetrahydronaphthalene-1-one (1), 4,8-dihydroxy-3-hydroxymethyl-4-methyl-1,4-dihydronaphthalene-1-one (2) and (rel)-4,8-dihydroxy-3-O-[-glucopyranosyl]hydroxymethyl-4-methyl-1,2,3,4-tetrahydronaphthalene-1-one (3). The compounds isolated appear to be derived via a shorter polyketide backbone than oxytetracycline (4), the normal end-product made by the parent of this strain. Compound 3 was the glucoside of 1 and must be formed as a post-PKS reaction by the activation of a glycosyl transferase, which has not been reported in this species before.


Immunomodulatory Polysaccharide From Chlorophytum Borivilianum Roots, Mayank Thakur, Paul Connellan, Myrna Deseo, Carol Morris, V Dixit Oct 2013

Immunomodulatory Polysaccharide From Chlorophytum Borivilianum Roots, Mayank Thakur, Paul Connellan, Myrna Deseo, Carol Morris, V Dixit

Dr Myrna A Deseo

No abstract provided.


Diarylheptanoid From Pleuranthodium Racemigerum With In Vitro Prostaglandin E2 Inhibitory And Cytotoxic Activity, Hans Wohlmuth, Myrna Deseo, Don Brushett, Dion Thompson, Graham Macfarlane, Lesley Stevenson, David Leach Oct 2013

Diarylheptanoid From Pleuranthodium Racemigerum With In Vitro Prostaglandin E2 Inhibitory And Cytotoxic Activity, Hans Wohlmuth, Myrna Deseo, Don Brushett, Dion Thompson, Graham Macfarlane, Lesley Stevenson, David Leach

Dr Myrna A Deseo

Bioactivity-guided fractionation of an ethanolic extract of the rhizome of Pleuranthodium racemigerum, a tropical Zingiberaceae species from North-eastern Australia, resulted in the isolation and structural elucidation of 1-(4″-methoxyphenyl)-7-(4′-hydroxyphenyl)-(E)-hept-2-ene (1), a new diarylheptanoid related to curcumin. Compound 1 was a fairly potent inhibitor of prostaglandin E2 production in 3T3 murine fibroblasts (IC50 ≈ 34 μM) and also displayed moderate cytotoxicity against this cell line (IC50 = 52.8 μM). The compound also demonstrated cytotoxic activity against the P388D1 murine lymphoblast cell line (IC50 = 117.0 μM) and four human cell lines: Caco-2 colonic adenocarcinoma (IC50 = 44.8 μM), PC3 prostate adenocarcinoma (IC50 …


A Comparative Study Of Seed Morphology In Relation To Desiccation Tolerance And Other Physiological Responses In 71 Eastern Australian Rainforest Species, Kim Hamilton, Catherine Offord, Peter Cuneo, Myrna Deseo Oct 2013

A Comparative Study Of Seed Morphology In Relation To Desiccation Tolerance And Other Physiological Responses In 71 Eastern Australian Rainforest Species, Kim Hamilton, Catherine Offord, Peter Cuneo, Myrna Deseo

Dr Myrna A Deseo

Seed characteristics were measured in 71 Eastern Australian rainforest species representing 30 families. Sensitivity to desiccation to low moisture contents (< 10%) occurred in 42% of species. We estimate, based on findings from 100 species from this present study and previously published reports, that 49% of Eastern Australian rainforest species have non-orthodox seeds. Germination level and time to 50% germination were not significantly different between desiccation sensitive (DS) and desiccation tolerant (DT) seeds. The estimation of seed desiccation sensitivity based on predictors is an important tool underpinning ex situ conservation efforts. Seed characteristics differed significantly between DS and DT seeds; …


Improvement Of Penile Erection, Sperm Count And Seminal Fructose Levels In Vivo And Nitric Oxide Release In Vitro By Ayurvedic Herbs, Mayank Thakur, Dion Thompson, Paul Connellan, Myrna Deseo, Carol Morris, V Dixit Oct 2013

Improvement Of Penile Erection, Sperm Count And Seminal Fructose Levels In Vivo And Nitric Oxide Release In Vitro By Ayurvedic Herbs, Mayank Thakur, Dion Thompson, Paul Connellan, Myrna Deseo, Carol Morris, V Dixit

Dr Myrna A Deseo

In the present study, the effect of four Vajikaran Rasayana herbs on penile erection, sperm count, seminal fructose content in vivo and nitric oxide (NO) release in vitro was assessed. Lyophilised aqueous extracts of Asparagus racemosus Willd. (AR), Chlorophytum borivilianum Sant. F. (CB), Curculigo orchioides Gaertn. (CO), and Dactylorhiza hatagirea (D. Don) Soo (DH) were orally administered at 100 mg/kg body weight to Wistar strain male albino rats. Penile erection index and sperm count were determined by visual observation; the seminal fructose concentration was measured spectrophotometrically using resorcinol reagent; and NO release was assessed in a mouse macrophage cell line …


Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati Oct 2013

Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati

Rajesh Pati

Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.


Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati Oct 2013

Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati

Rajesh Pati

Aegle marmelos Corr., belongs to family Rutaceae, is more prized for its pharmacological virtues than its edible quality. Because of pharmacological importance, it’s become potential candidate for developing transgenics to enhance its medicinal properties. Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage, and field established plants).


Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati Oct 2013

Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati

Rajesh Pati

A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.