Digital Commons Network^™

Mir319a Targeting Of Tcp4 Is Critical For Petal Growth And Development In Arabidopsis, Anwesha Nag, Stacey King, Thomas Jack

Dartmouth Scholarship

In a genetic screen in a drnl-2 background, we isolated a loss-of-function allele in miR319a (miR319a¹²⁹). Previously, miR319a has been postulated to play a role in leaf development based on the dramatic curled-leaf phenotype of plants that ectopically express miR319a (jaw-D). miR319a¹²⁹ mutants exhibit defects in petal and stamen development; petals are narrow and short, and stamens exhibit defects in anther development. The miR319a¹²⁹ loss-of-function allele contains a single-base change in the middle of the encoded miRNA, which reduces the ability of miR319a to recognize targets. Analysis of the expression patterns of the …

Non-Antibiotic Selection Systems For Soybean Somatic Embryos: The Lysine Analog Aminoethyl-Cysteine As A Selection Agent, Suryadevara S. Rao, Lewamy Mamadou, Matt Mcconnell, Raghuveer Polisetty, Prachuab Kwanyuen, David Hildebrand

Plant and Soil Sciences Faculty Publications

BACKGROUND: In soybean somatic embryo transformation, the standard selection agent currently used is hygromycin. It may be preferable to avoid use of antibiotic resistance genes in foods. The objective of these experiments was to develop a selection system for producing transgenic soybean somatic embryos without the use of antibiotics such as hygromycin.

RESULTS: When tested against different alternate selection agents our studies show that 0.16 microg/mL glufosinate, 40 mg/L isopropylamine-glyphosate, 0.5 mg/mL (S-(2 aminoethyl)-L-cysteine) (AEC) and the acetolactate synthase (ALS) inhibitors Exceed and Synchrony both at 150 microg/mL inhibited soybean somatic embryo growth. Even at the concentration of 2 mg/mL, …

Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Sichul Lee, Jeff C. Chiecko, Sun A. Kim, Elsbeth L. Walker, Youngsook Lee, Mary Lou Guerinot, Gyhheung An

Dartmouth Scholarship

Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe³⁺; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to β-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15:green …

Plant Vaccines: An Immunological Perspective., Douglas C. Hooper

Department of Cancer Biology Faculty Papers

The advent of technologies to express heterologous proteins in planta has led to the proposition that plants may be engineered to be safe, inexpensive vehicles for the production of vaccines and possibly even vectors for their delivery. The immunogenicity of a variety of antigens of relevance to vaccination expressed in different plants has been assessed. The purpose of this article is to examine the utility of plant-expression systems in vaccine development from an immunological perspective.

Impacts Of Plant Size, Density, Herbivory, And Desease On Native Platte Thistle (Cirsium Canescens), Deidra Jacobsen

Department of Environmental Studies: Undergraduate Student Theses

Abstract. Based on prior field observations, we hypothesized that individual and interacting effects of plant size, density, insect herbivory, and especially fungal disease, influenced seedling and juvenile plant growth in native Platte thistle populations (Cirsium canescens Nutt.). We worked at Arapaho Prairie in the Nebraska Sandhills (May - August 2007), monitoring plant growth, insect damage, and fungal infection within different density thistle patches. In the main experiment, we sprayed half of test plants in different density patches with fungicide (Fungonil© Bonide, containing chlorothalonil) and half with a water control. Fungal infection rates were very low, so we found no difference …

Evaluating Hazelnut Cultivars For Yield, Quality And Disease Resistance, Sam Tobin

Department of Environmental Studies: Undergraduate Student Theses

ABSTRACT This long term study focuses on testing various hazelnut cultivars for yield, nut quality and disease resistance. There are various cultivars that are being tested for these desired traits but only the Grand Traverse and Skinner will be applicable for the results of this localized study. The desired traits of commercial nut production are best matched by these two cultivars. Results from previous harvests will be used to draw trends to recommend commercially functional cultivars in Eastern Nebraska.

Method Utilizing The Tobacco Phylloplanin Promoter For Expression Of Nucleic Acids As Gene Products Directed To Aerial Surfaces Of Plants, George J. Wagner, Ryan W. Shepard

Plant and Soil Sciences Faculty Patents

A method and system is provided for generating excreted gene products in a plant which includes generating a nucleic acid fusion construct comprising a phylloplanin promoter and a selected non-phylloplanin nucleic acid sequence whose transcription product is to be expressed and delivered to an aerial surface of a plant. The method includes transfecting plant cells with the nucleic acid fusion construct and allowing the plant cells to express the non-phylloplanin nucleic acid sequence transcription product which is then excreted from the plant cells to the aerial surfaces.

Mountain Beebalm In The Landscape, Heidi Kratsch, Graham Hunter

Gardening

No abstract provided.

Firecracker Penstemon In The Landscape, Heidi Kratsch, Graham Hunter

Gardening

No abstract provided.

Prince's Plume In The Landscape, Heidi Kratsch, Graham Hunter

All Current Publications

No abstract provided.