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Assessment Of Genetic Variation Of Natural Populations Of Wild Cherry (Prunus Avium L.)Via Ssr Markers, Burcu Uzan Eken, Emrah Kirdök, Ercan Veli̇oğlu, Yelda Özden Çi̇ftçi̇
Assessment Of Genetic Variation Of Natural Populations Of Wild Cherry (Prunus Avium L.)Via Ssr Markers, Burcu Uzan Eken, Emrah Kirdök, Ercan Veli̇oğlu, Yelda Özden Çi̇ftçi̇
Turkish Journal of Botany
Wild cherry (Prunus avium L, syn. Cerasus avium L. Moench.) is a widely spread forest tree that has ecological and economical importance. However, the genetic diversity of this species is threatened for many reasons. Therefore, a breeding and conservation program should be established in order to minimise the loss of genetic diversity. In this study, we aimed to understand the genetic structure of 440 individual wild cherries sampled from 22 different populations in Turkey using 10 SSR molecular markers. With the molecular variance analysis, we found that the genetic diversity within the population is approximately 88.5% and the genetic diversity …
Genetic Diversity And Population Structure Analysis For Ampelopsis Grossedentata Using Microsatellite Markers, Xiao Ying Li, L Wei
Genetic Diversity And Population Structure Analysis For Ampelopsis Grossedentata Using Microsatellite Markers, Xiao Ying Li, L Wei
Turkish Journal of Botany
Ampelopsis grossedentata is an important national medicine in China. It is mainly used commonly by the Yao, Miao, Dong, and other ethnic minorities of China. The current literature, however, contains no study on the molecular genetics and taxonomy of A. grossedentata, which had led to potential problems in the conservation and utilization of this resource. Therefore, the present study was aimed to use simple sequence repeat (SSR) markers for assessing the levels and the distribution of genetic diversity in a sample set of 225 individuals from nine populations of A. grossedentata in China. The genetic differentiation among the nine …
Weedy Rice Conserved Ex Situ Characterized Using Morphological And Simple Sequence Repeat (Ssr) Markers, Rubylyn Mijan Infante, Maria Elizabeth B. Naredo, Merlyn S. Mendioro
Weedy Rice Conserved Ex Situ Characterized Using Morphological And Simple Sequence Repeat (Ssr) Markers, Rubylyn Mijan Infante, Maria Elizabeth B. Naredo, Merlyn S. Mendioro
The Philippine Agricultural Scientist
A clear understanding of genetic diversity in weedy rice is important in improving protocols for its conservation, ex situ. To this end, Asian collections of weedy rice together with accessions of the wild AA genome species Oryza nivara Sharma et Shastry and Oryza rufipogon Griffith, and the cultivated species, Oryza sativa L. were characterized using 16 qualitative morphological traits and 18 SSR markers. The Shannon-Weaver index (H’) revealed higher diversity in the weedy (mean H’ = 0.76) and wild O. nivara (mean H’ = 0.79) and O. rufipogon samples (mean H’ = 0.69) compared to the cultivated …
Genetic Structure, Differentiation, And Slight Evidence For No Selective Neutrality Of Some Ssr Markers In Populations Of Medicago Ciliaris (L.) Krock And Medicago Intertexta (L.)Mill Species, Fella Abdous, Fatima Zohra Fyad-Lameche
Genetic Structure, Differentiation, And Slight Evidence For No Selective Neutrality Of Some Ssr Markers In Populations Of Medicago Ciliaris (L.) Krock And Medicago Intertexta (L.)Mill Species, Fella Abdous, Fatima Zohra Fyad-Lameche
Turkish Journal of Botany
Medicago ciliaris (L.) Krock and Medicago intertexta (L.) Mill are most often found in saline regions and are considered as potential resources for genes related to salinity resistance. To explore the genetic diversity of these two species by mean of nrSSR (simplesequence repeats) markers, 11 accessions originated from different regions in north of Africa and Middle East were chosen. Ten among 14 markers turned out to be polymorphic and gave 72 alleles. Eight markers were very informative with PIC values > 0.5, and three of them were found to be nonneutral. Allelic richness was limited with an average number of alleles …
Genetic Structure And Diversity Of Adonis L. (Ranunculaceae) Populations Collected From Turkey By Inter-Primer Binding Site (Ipbs) Retrotransposon Markers, Arash Hossein Pour, Faruk Karahan, Emre İlhan, Ahmet İlçi̇m, Kami̇l Hali̇loğlu
Genetic Structure And Diversity Of Adonis L. (Ranunculaceae) Populations Collected From Turkey By Inter-Primer Binding Site (Ipbs) Retrotransposon Markers, Arash Hossein Pour, Faruk Karahan, Emre İlhan, Ahmet İlçi̇m, Kami̇l Hali̇loğlu
Turkish Journal of Botany
The genus Adonis L. is a member of Ranunculaceae and consists of perennial and annual herbaceous plants included in the tribe Adonideae under the subfamily Ranunculoideae. Botanically, Ranunculaceae comprises vital medicinal plants. Molecular markers are one of the most effective tools for exploring genetic variation that can enhance breeding efficiency. To identify the genetic diversity of 62 Adonis ecotypes collected from different regions in Turkey, the interprimer binding site (iPBS) retrotransposon system was used. Of the 83 iPBS primers used, 10 provided sufficient polymorphic data, generating a total of 204 alleles. The number of iPBS bands per individual was 3.29, …
Genetic Diversity And Agronomic Performance Of Slovenian Landraces Of Proso Millet (Panicum Miliaceum L.), Marko Flajsman, Natasa Stajner, Darja Kocjan Acko
Genetic Diversity And Agronomic Performance Of Slovenian Landraces Of Proso Millet (Panicum Miliaceum L.), Marko Flajsman, Natasa Stajner, Darja Kocjan Acko
Turkish Journal of Botany
Proso millet (Panicum miliaceum L.) has many favored nutritional and agronomic traits, which makes it appropriate for cultivation and consumption all around the world. Genomic resources for proso millet are still very limited but the set of genomic data is improving. In this study, we genotyped six Slovenian landraces of proso millet (P. miliaceum L.) along with one Slovene autochthonous cultivar, Son?ek. The chosen set of 11 SSR markers showed that there is low overall heterozygosity (0.561) among Slovenian landraces of proso millet. However, we were able to determine distinct groups on the dendrogram for different landraces and the cultivar …
Plastid Dna Variation Of The Endemic Species Oxytropis Glandulosa Turcz. (Fabaceae), Alla Kholina, Marina Kozyrenko, Elena Artyukova, Denis Sandanov, Inessa Selyutina, Daba Chimitov
Plastid Dna Variation Of The Endemic Species Oxytropis Glandulosa Turcz. (Fabaceae), Alla Kholina, Marina Kozyrenko, Elena Artyukova, Denis Sandanov, Inessa Selyutina, Daba Chimitov
Turkish Journal of Botany
Oxytropis glandulosa Turcz. (Fabaceae) is a rare perennial plant endemic to Buryatia (Russia). We sequenced three intergenic spacers (the psbA−trnH, trnL−trnF, and trnS−trnG) of chloroplast DNA (cpDNA) in specimens of four populations from two geographic regions (Barguzin and Yeravna depressions) within this species range. The levels of haplotype and nucleotide diversity varied in the range of 0.133−0.911 and 0.0002−0.0059, respectively. The highest values of these parameters are characteristic of populations located in the Barguzin depression. Variable sites detected within the intergenic spacers allowed the identification of eleven haplotypes; nine of them were found in the populations from the Barguzin depression …
Molecular Characterization Of Edible Pea Through Est-Ssr Markers, Mohammad Nisar, Asaf Khan, Syed Fazal Wadood, Aftab Ali̇ Shah, Fati̇h Hanci
Molecular Characterization Of Edible Pea Through Est-Ssr Markers, Mohammad Nisar, Asaf Khan, Syed Fazal Wadood, Aftab Ali̇ Shah, Fati̇h Hanci
Turkish Journal of Botany
The genetic diversity among 23 newly developed homosegregate pea lines (Pisum sativum L.) was assessed with a total of 13 expressed sequence tag (EST) based-simple sequence repeat (SSR) markers. The percentages of amplified and nonamplified primers were 92% and 8%, respectively, and 58.33% of the used primers gave the PCR product within the reported size range while 41.66% of primers gave a different product size. Polymorphism information content (PIC), major allele frequency, and variation in genetic diversity were calculated. The PIC ranged from 0.32 to 0.63 with an average of 0.50. Major allele frequency ranged from 0.48 to 0.78 with …
Assessment Of Genetic Diversity Of Rose Genotypes Using Issr Markers, Ti̇jen Oğraş, Evren Koban Baştanlar, Özge Karakaş Meti̇n, İrfan Kandemi̇r, Hasan Özçeli̇k
Assessment Of Genetic Diversity Of Rose Genotypes Using Issr Markers, Ti̇jen Oğraş, Evren Koban Baştanlar, Özge Karakaş Meti̇n, İrfan Kandemi̇r, Hasan Özçeli̇k
Turkish Journal of Botany
The genus Rosa L. (Rosaceae) is an important ornamental crop with a high economic value in floriculture, cosmetics, and pharmaceutical industries. Turkey is an important country in rose cultivation with unique species and cultivars, especially for rose oil production. Breeding programs of different rose genotypes are crucial for the development of new commercial varieties. In this study, genetic relationships among the old rose cultivars locally grown in Isparta were assessed by DNA fingerprinting. Inter-simple sequence repeats markers (ISSRs) were used to investigate the genetic diversity among nineteen locally grown rose genotypes. Nineteen ISSR primers yielded a total of 413 scorable …
Genetic Diversity And Molecular Characterization Of Natural Pancratium Maritimum L. Populations By Dna Markers, Ceren Eli̇bol, Behi̇ye Banu Bi̇lgen
Genetic Diversity And Molecular Characterization Of Natural Pancratium Maritimum L. Populations By Dna Markers, Ceren Eli̇bol, Behi̇ye Banu Bi̇lgen
Turkish Journal of Botany
Bulbous plants play an important role in Turkey's biodiversity due to their great potential use in various industries. Sea daffodils (Pancratium maritimum), one aspect of Turkey's biological richness, represent an important bulbous plant that generally spreads on sand dunes and can be seen only on the Mediterranean coast and on its certain beaches in our country. In this study, the genetic structure and genetic diversity of four natural P. maritimum populations have been determined by RAPD and nrSSR primers. Eight RAPD and four nrSSR loci were analyzed. All RAPD and nrSSR loci, except SSR-20, were found to be polymorphic. Genetic …
Estimating Genetic Diversity Among Selected Cotton Genotypes And The Identificationof Dna Markers Associated With Resistance To Cotton Leaf Curl Disease, Ammad Abbas, Muhammad Atif Iqbal, Mehboob-Ur Rahman, Andrew H. Paterson
Estimating Genetic Diversity Among Selected Cotton Genotypes And The Identificationof Dna Markers Associated With Resistance To Cotton Leaf Curl Disease, Ammad Abbas, Muhammad Atif Iqbal, Mehboob-Ur Rahman, Andrew H. Paterson
Turkish Journal of Botany
To the extent of our knowledge, applications of DNA markers in marker-assisted breeding of cotton are handicapped due to low genetic diversity in cotton germplasm. Cotton leaf curl disease, a disease of viral origin, has substantially depressed cotton production in Pakistan, and this disease is also an emerging threat to the neighboring cotton-growing countries like China and India. The present study was designed to identify DNA markers, predominately simple sequence repeats (SSRs), associated with tolerance and/or resistance to the disease. Based upon 2 years of disease-screening field experiments, a total of 10 cotton genotypes (five highly tolerant, four highly susceptible, …
Transferability Of Barley Retrotransposon Primers To Analyze Genetic Structure In Iranian Hypericum Perforatum L. Populations, Razea Asadkhani Mamaghani, Seyed Abolghasem Mohammadi, Saeid Aharizad
Transferability Of Barley Retrotransposon Primers To Analyze Genetic Structure In Iranian Hypericum Perforatum L. Populations, Razea Asadkhani Mamaghani, Seyed Abolghasem Mohammadi, Saeid Aharizad
Turkish Journal of Botany
Transferability of barley retrotransposon primers was investigated to analyze population structure in St. John's wort (Hypericum perforatum L.) based on inter-retrotransposon amplified polymorphism (IRAP). Seven long terminal repeat (LTR) retrotransposon primers derived from the barley genome were used to detect genetic polymorphism in eight Iranian populations and three cultivars (Helos, New Stem, and Topaz) of H. Perforatum based on IRAP analysis. Nine possible LTR primers/primer combinations successfully amplified fragments from the H. Perforatum genome. In total, 311 bands of 100-3000 base pairs were amplified, of which 244 were polymorphic. The number of polymorphic fragments ranged from 10 (Nikita/5'LTR-2) to 57 …
Reproduction And Diversity Of The Endangered Salix Lapponum L. Populations In Eastern Poland, Magdalena Pogorzelec, Katarzyna Glebocka, Barbara Hawrylak-Nowak, Marzena Parzymies
Reproduction And Diversity Of The Endangered Salix Lapponum L. Populations In Eastern Poland, Magdalena Pogorzelec, Katarzyna Glebocka, Barbara Hawrylak-Nowak, Marzena Parzymies
Turkish Journal of Botany
The main aim of the study was to increase the knowledge on the ecology and biology of the boreal relict Salix lapponum L., an endangered plant in Poland, in order to create an effective protection program. An important aspect was to determine the within- and among-population genetic diversity of the studied species in order to evaluate whether the basic stages of the generative reproduction cycle in the populations were correct. The results confirmed that S. lapponum pollen viability and germinability, for both fresh and stored pollen, were high and so were the seed germination ability and dynamics. All individuals were …
The Assessment Of Genetic Diversity Of Castanea Species By Rapd, Aflp, Issr, And Ssr Markers, Sofiane Abdelhamid, Cong-Linh Lê, Marco Conedera, Philippe Küpfer
The Assessment Of Genetic Diversity Of Castanea Species By Rapd, Aflp, Issr, And Ssr Markers, Sofiane Abdelhamid, Cong-Linh Lê, Marco Conedera, Philippe Küpfer
Turkish Journal of Botany
Castanea Mill. (chestnut) is a multipurpose deciduous tree and member of the family Fagaceae, widely distributed throughout North America, Europe, and Asia. The evaluation of the genetic diversity of chestnut species is crucial for the effective conservation of this economically and ecologically valuable tree. In this study, we applied 4 DNA markers to detect the genetic variability among and within Castanea species and to compare the effectiveness of each system in estimating genetic variation. We amplified 106 random amplified polymorphic DNA (RAPD), 228 amplified fragment length polymorphism (AFLP), 42 intersimple sequence repeat (ISSR), and 36 simple sequence repeat (SSR) polymorphic …
Identification Of Stable Sources For Surrogate Traits In Arachis Glabrata And Marker-Trait Association For Tolerance To Water Deficit Stress, Sandip Kumar Bera, Ajay Basapur Chandrashekar, Sneha Patel, Vrajlal Kanji Sojitra, Anil Maurya
Identification Of Stable Sources For Surrogate Traits In Arachis Glabrata And Marker-Trait Association For Tolerance To Water Deficit Stress, Sandip Kumar Bera, Ajay Basapur Chandrashekar, Sneha Patel, Vrajlal Kanji Sojitra, Anil Maurya
Turkish Journal of Botany
Diploid and tetraploid wild relatives of cultivated peanut are genetically diverse, providing rich sources of genetic resources for tapping resistance to various biotic and abiotic stresses. Arachis glabrata Benth. is a tetraploid (2n = 40) rhizomatous species that is reported to be tolerant to water deficit stress. Identification of A. glabrata accessions with high SPAD chlorophyll meter reading (SCMR) and low specific leaf area (SLA) values would be an option for improving water deficit stress tolerance in peanut. An investigation was undertaken to study the genetic diversity of A. glabrata accessions using molecular markers as well as surrogate traits (SLA …
Molecular Variability Of Plantain Ecotypes From The Genus Musa (Musaceae), Rakeshkumar Choudhary, Raghunath Keshavachandran, Rema Menon, Ganesh Khalekar, Nirbhay Singh, Divya Maruthiyottu
Molecular Variability Of Plantain Ecotypes From The Genus Musa (Musaceae), Rakeshkumar Choudhary, Raghunath Keshavachandran, Rema Menon, Ganesh Khalekar, Nirbhay Singh, Divya Maruthiyottu
Turkish Journal of Botany
Twelve plantain ecotypes were characterized for molecular variability using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Out of the 60 RAPD and 40 ISSR primers tested, 16 RAPD and 14 ISSR primers were found polymorphic, and were used for molecular profiling of the plantain ecotypes. The average number of bands per primer, average number of polymorphic bands per primer, mean polymorphic information content (PIC) value, resolving power (Rp), and marker indices (MI) for RAPD primers were 8.63, 3.25, 0.87, 15.49, and 2.86, whereas the respective values for the ISSR assay were 7.93, 4.14, 0.85, 12.49, …
Assessment Of Genetic Diversity And Phylogenetic Relationships Of Endangered Endemic Plant Barbarea Integrifolia Dc. (Brassicaceae) In Turkey, Ertuğrul Fi̇li̇z, Etem Osma, Ali̇ Kandemi̇r, Hüseyi̇n Tombuloğlu, Guzi̇n Tombuloglu, Seda Bi̇rbi̇lener, Mehtap Aydin
Assessment Of Genetic Diversity And Phylogenetic Relationships Of Endangered Endemic Plant Barbarea Integrifolia Dc. (Brassicaceae) In Turkey, Ertuğrul Fi̇li̇z, Etem Osma, Ali̇ Kandemi̇r, Hüseyi̇n Tombuloğlu, Guzi̇n Tombuloglu, Seda Bi̇rbi̇lener, Mehtap Aydin
Turkish Journal of Botany
Barbarea integrifolia DC. (Brassicaceae) is an endangered and endemic species located in Erzincan and Gümüşhane provinces of Turkey. In total, 27 individuals from 2 natural populations were assessed using the random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method coupled with sequence analysis of the internal transcribed spacer (ITS1) rDNA region. Genetic accuracy of RAPD-PCR was tested with 25 RAPD primers, and it resulted in 115 clear and reproducible DNA fragments from 13 RAPD primers. Among these, 76 (66.1%) fragments were found to be polymorphic. According to the ITS1 analysis of B. integrifolia populations, the plants exhibited a relatively poor …
Genetic Diversity Among Nile Delta Isolates Of Rhizoctonia Solani Kühn Based On Pathogenicity, Compatibility, Isozyme Analysis And Total Protein Pattern, Yehia A.G. Mahmoud, Reda M. Gaafar, H. M. Mubarak
Genetic Diversity Among Nile Delta Isolates Of Rhizoctonia Solani Kühn Based On Pathogenicity, Compatibility, Isozyme Analysis And Total Protein Pattern, Yehia A.G. Mahmoud, Reda M. Gaafar, H. M. Mubarak
Turkish Journal of Botany
The present study obtained 12 isolates from Rhizoctonia solani Kühn isolated from Cotton L., Trifolium L. and Vicia faba L. from different localities in the Nile Delta of Egypt. All strains were pathogenic and caused seed rot, wilt, stunting, and pre-emergence and post-emergence damping-off. The isolated strains produced different forms of infection cushions that ensure the pathogenicity of these strains. SDS-PAGE of the 12 R. solani isolates showed that although the R. solani isolates were isolated from very divergent host plants, the total soluble protein patterns among them were similar. Isozyme analysis revealed higher polymorphism among R. solani isolates using …