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Evaluation Of Various Sunflower ( Helianthus Annuus L.) Genotypes For Agrobacterium Tumefaciens-Mediated Gene Transfer, Ekrem Gürel, Kemal Kazan
Evaluation Of Various Sunflower ( Helianthus Annuus L.) Genotypes For Agrobacterium Tumefaciens-Mediated Gene Transfer, Ekrem Gürel, Kemal Kazan
Turkish Journal of Botany
In this work, 10 sunflower ( Helianthus annuus L.) genotypes were evaluated for their suitability for Agrobacterium tumefaciens-mediated gene transfer based on detection of a reported gene expression (GUS). A number of factors including the type of shoot-tip explant (split vs. intact), bacterial strain/binary vector combinations and wounding intact shoot-tips by particle bombardment were examined. Genotypes showed significant differences in their transformation efficiencies ranging from 0.0 to 82.7% GUS positive explants, hybrid genotypes being more responsive to Agrobacterium infection than inbred lines. Use of split shoot-tip explants did not increase the transformation efficiency over intact explants but the AGL-1/pKIWI strain/vector …
Expression And Inheritance Of Gus Gene In Transgenic Tobacco Plants, Ti̇jen Talas Oğraş, Nermi̇n Gözükirmizi
Expression And Inheritance Of Gus Gene In Transgenic Tobacco Plants, Ti̇jen Talas Oğraş, Nermi̇n Gözükirmizi
Turkish Journal of Botany
Agrobacterium tumefaciens-mediated transformation of tobacco leaf discs was performed with plant transformation vec-tors of pBI 121 and pGus-Int. Both carry the neomycin phosphotransferase II ( npt II) gene and, unlike the pBI 121, pGus-Int car-ries a portable plant intron inside the beta-glucuronidase (GUS) gene. In addition to the integration of the marker genes into the genomes of primary transformants, R1 and R2 progenies were confirmed by polymerase chain reaction (PCR) and Southern blot analyses. Histochemical and fluorometric analyses were also performed to determine the activity of the GUS gene. Segregation of progenies on kanamycin-resistance trait was also shown as evidence …
Agrobacterium Tumefaciens-Mediated Transformation Of Sesame (Sesamum Indicuml.), Kemal Meli̇h Taşkin, A. Gülhan Ercan, Kenan Turgut
Agrobacterium Tumefaciens-Mediated Transformation Of Sesame (Sesamum Indicuml.), Kemal Meli̇h Taşkin, A. Gülhan Ercan, Kenan Turgut
Turkish Journal of Botany
Sesamum indicum L. cv. Özberk was infected with various Agrobacterium tumefaciens strains to screen susceptibility to infection by Agrobacteria. Following infection, tumorigenesis was efficient with wild type A. tumefaciens strains, A281 and A136 NC, and transgenic cells were obtained with disarmed A. tumefaciens strain LBA4404/pBI121, harbouring a reporter gene. At the wound sites, tumorigenesis induced by the succinamopine strain A281 was more extensive than by the octopine strain A136 NC.