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United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
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Multiplex Real-Time Pcr For Detection, Identification And Quantification Of ‘Candidatus Liberibacter Solanacearum’ In Potato Plants With Zebra Chip, Wenbin Li, Jorge A. Abad, Ronald D. French-Monar, John Rascoe, Aimin Wen, Neil C. Gudmestad, Gary A. Secor, Ing-Ming Lee, Yongping Duan, Laurene Levy
Multiplex Real-Time Pcr For Detection, Identification And Quantification Of ‘Candidatus Liberibacter Solanacearum’ In Potato Plants With Zebra Chip, Wenbin Li, Jorge A. Abad, Ronald D. French-Monar, John Rascoe, Aimin Wen, Neil C. Gudmestad, Gary A. Secor, Ing-Ming Lee, Yongping Duan, Laurene Levy
United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications
The new Liberibacter species, ‘Candidatus Liberibacter solanacearum’ (Lso) recently associated with potato/tomato psyllid-transmitted diseases in tomato and capsicum in New Zealand, was found to be consistently associated with a newly emerging potato zebra chip (ZC) disease in Texas and other southwestern states in the USA. A species-specific primer LsoF was developed for both quantitative real-time PCR (qPCR) and conventional PCR (cPCR) to detect and quantify Lso in infected samples. In multiplex qPCR, a plant cytochrome oxidase (COX)-based probe-primer set was used as a positive internal control for host plants, which could be used to reliably access the DNA extraction …