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- Method (5)
- Alkaloid (4)
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- P450 genes (4)
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Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nornicotine and N'-nitrosonomicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic con- version of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use …
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nornicotine and N'-nitrosonomicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balazs Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nomicotine and N'-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …
Methods For Increasing Renewable Oil Productions, David Hildebrand, Runzhi Li, Tomoko Hatanaka
Methods For Increasing Renewable Oil Productions, David Hildebrand, Runzhi Li, Tomoko Hatanaka
Plant and Soil Sciences Faculty Patents
Methods of increasing renewable oil production are provided and include transforming a plant cell with an isolated nucleic acid encoding a Vernonia galamensis diacylglycerol acyltransferase (V gDGAT) polypeptide, where the expression of the V gDGAT polypeptide increases an amount of renewable oil in the plant. Transgenic plant cells comprising an isolated nucleic acid encoding a Vernonia galamensis diacylglycerol acyl transferase 1 (V gDGATl) polypeptide are further provided.
Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan T. Greenhagen
Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan T. Greenhagen
Plant and Soil Sciences Faculty Patents
The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
Polypeptides, Nucleic Acid Molecules, And Methods For Synthesis Of Triterpenes, Joe Chappell, Thomas D. Niehaus, Shigeru Okada, Timothy P. Devarenne, David Watt
Polypeptides, Nucleic Acid Molecules, And Methods For Synthesis Of Triterpenes, Joe Chappell, Thomas D. Niehaus, Shigeru Okada, Timothy P. Devarenne, David Watt
Plant and Soil Sciences Faculty Patents
This application relates to the polypeptides, nucleic acid molecules, vectors, transfected cells, and methods for synthesis of triterpenes, including botryococcene.
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Plant and Soil Sciences Faculty Patents
Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring …
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Plant and Soil Sciences Faculty Patents
The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
B. Braunii, Race B Gene For A Triterpene Methyltransferase Enzyme And Uses Thereof, Joe Chappell, Okada Shigeru, Scott Kinison, Tom Niehaus
B. Braunii, Race B Gene For A Triterpene Methyltransferase Enzyme And Uses Thereof, Joe Chappell, Okada Shigeru, Scott Kinison, Tom Niehaus
Plant and Soil Sciences Faculty Patents
Provided is an isolated polypeptide having triterpene methyltransferase activity. Also provided is an isolated nucleic acid molecule that encodes the triterpene methyltransferase polypeptides; a vector comprising the nucleic acid molecules that encode the triterpene methyltransferase polypeptides; and a host cell(s) transfected with the aforementioned nucleic acid molecule or vector. In another aspect, a method of producing a methylated triterpene is provided. The method comprises providing a metabolizable carbon source to a host cell transfected with a nucleic acid molecule that encodes a triterpene methyltransferase under conditions sufficient for production of a methylated triterpene. The method optionally further comprises isolating the …
Botryoccocus Braunii Triterpene Synthase Proteins And Nucleic Acid Molecules, And Methods For Their Use, Joe Chappell, Okada Shigeru, Tom Neihaus, Tim Devarenne
Botryoccocus Braunii Triterpene Synthase Proteins And Nucleic Acid Molecules, And Methods For Their Use, Joe Chappell, Okada Shigeru, Tom Neihaus, Tim Devarenne
Plant and Soil Sciences Faculty Patents
This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.
Early Flowering Mutant Chia And Uses Thereof, David Hildebrand, Watchareewan Jamboonsri, Timothy Phillips
Early Flowering Mutant Chia And Uses Thereof, David Hildebrand, Watchareewan Jamboonsri, Timothy Phillips
Plant and Soil Sciences Faculty Patents
The invention relates to early flowering Chia (Salvia hispanica L.) strains that are suitable for culturing in a temperate area for seed production. Mutations are introduced to wild type chia seeds. Desired mutant progeny having normal appearance and an altered flora organ development is subsequently identified.
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Plant and Soil Sciences Faculty Patents
The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
Diacylglycerol Acyltransferase Sequences And Related Methods, David Hildebrand, Runzhi Li, Tomoko Hatanaka
Diacylglycerol Acyltransferase Sequences And Related Methods, David Hildebrand, Runzhi Li, Tomoko Hatanaka
Plant and Soil Sciences Faculty Patents
Isolated nucleic acid and amino acid sequences encoding a diacylglycerol acyltransferase 2 (DGAT2) polypeptide are provided. Vectors and transgenic cells that include a nucleic acid sequence encoding a DGAT2 polypeptide are also described. Further provided are methods of producing an epoxy fatty acid by transforming a cell with a first isolated nucleic acid that encodes a diacylglycerol acyltransferase polypeptide and a second isolated nucleic acid that encodes an epoxygenase polypeptide, such that expression of the diacylglycerol acyltransferase polypeptide and the epoxygenase polypeptide increases an amount of epoxy fatty acid in the cell.
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Plant and Soil Sciences Faculty Patents
Provided is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous, isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous, isoprenoid synthase. Also provided is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned heterologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring …
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Cytochrome P450s And Uses Thereof, Joe Chappell, Lyle F. Ralston
Plant and Soil Sciences Faculty Patents
The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds.
Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Plants, George Wagner, Ryan Sheperd
Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Plants, George Wagner, Ryan Sheperd
Plant and Soil Sciences Faculty Patents
The present invention relates to plant proteins produced by a plant's epidermal layer that contribute to the innate pest/disease resistance of the plant (“phylloplanins”), compositions comprising the phylloplanins and methods of using them. In particular the methods relate to inhibiting or preventing microbial, e.g., fungal or bacterial, growth on a subject, organism or surface by administering a phylloplanin. The invention also relates to transformed host cells that produce phylloplanins, and to transgenic plants producing phylloplanins conferring increased resistance to microbial infections/growth.
Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen
Sesquiterpene Synthase Gene And Protein, Joe Chappell, Bryan Greenhagen
Plant and Soil Sciences Faculty Patents
The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention.
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balaz Siminszky, Steven W. Bowen, Lily Gavilano
Alteration Of Tobacco Alkaloid Content Through Modification Of Specific Cytochrome P450 Genes, Ralph E. Dewey, Balaz Siminszky, Steven W. Bowen, Lily Gavilano
Plant and Soil Sciences Faculty Patents
Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in …
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Chimeric Isoprenoid Synthases And Uses Thereof, Joe Chappell, Kyoungwhan Back
Plant and Soil Sciences Faculty Patents
Disclosed is a chimeric isoprenoid synthase polypeptide including a first domain from a first isoprenoid synthase joined to a second domain from a second, heterologous isoprenoid synthase, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced in the absence of the second domain of the second, heterologous isoprenoid synthase. Also disclosed is a chimeric isoprenoid synthase polypeptide including an asymmetrically positioned homologous domain, whereby the chimeric isoprenoid synthase is capable of catalyzing the production of isoprenoid reaction products that are not produced when the domain is positioned at its naturally-occurring …
Fungal Desaturases And Related Methods, David F. Hildebrand, Suryadevara S. Rao, John Thoguru
Fungal Desaturases And Related Methods, David F. Hildebrand, Suryadevara S. Rao, John Thoguru
Plant and Soil Sciences Faculty Patents
The presently-disclosed subject matter provides isolated nucleic acid and amino acid sequences encoding mushroom desaturase polypeptides that are active with both palmitic and stearic acid, as well as vectors and transgenic plant cells comprising nucleic acids of the presently-disclosed subject matter. The presently-disclosed subject matter further provides methods of producing monounsaturated fatty acids, such as palmitoleic acid (16:1), and monounsaturated fatty acids prepared by the methods disclosed herein.
Transformed Plants Accumulating Terpenes, Joe Chappell, Shuiqin Wu, Michel Schalk, Anthony Clark
Transformed Plants Accumulating Terpenes, Joe Chappell, Shuiqin Wu, Michel Schalk, Anthony Clark
Plant and Soil Sciences Faculty Patents
The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.
Botryoccocus Braunii Triterpene Synthase Proteins And Nucleic Acid Molecules, And Methods For Their Use, Joe Chappell, Shigeru Okada, Tom Niehaus, Tim Devarenne
Botryoccocus Braunii Triterpene Synthase Proteins And Nucleic Acid Molecules, And Methods For Their Use, Joe Chappell, Shigeru Okada, Tom Niehaus, Tim Devarenne
Plant and Soil Sciences Faculty Patents
This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.
Method For Reducing Nitrosamines In Tobacco, Terry Thomas, John Brandon, William A. Bailey, Thomas A. Losty
Method For Reducing Nitrosamines In Tobacco, Terry Thomas, John Brandon, William A. Bailey, Thomas A. Losty
Plant and Soil Sciences Faculty Patents
Nitrosamines are reduces in tobacco by treating a whole tobacco lead with ferulic acid. Treatment is conducted by applying an aqueous solution of ferulic acid to the whole tobacco lead immediately before, at, or after harvesting, or fire curing the whole tobacco lead with wood smoke containing the ferulic acid.
Methods For Screening For Genes And Small Molecules That Activate Mammalian Receptor Proteins, Deane L. Falcone, John M. Littleton
Methods For Screening For Genes And Small Molecules That Activate Mammalian Receptor Proteins, Deane L. Falcone, John M. Littleton
Plant and Soil Sciences Faculty Patents
Methods for screening mutations that affect the synthesis of plant small molecules or compounds capable of activating a mammalian nuclear receptor protein and systems for rapidly assigning functionality to genes that regulate plant secondary metabolism are provided.
Genetic Insulator For Preventing Influence By Another Gene Promoter, Susheng Gan, Mingtang Xie
Genetic Insulator For Preventing Influence By Another Gene Promoter, Susheng Gan, Mingtang Xie
Plant and Soil Sciences Faculty Patents
A 16 bp polynucleotide sequence of Arabidopsis thaliana is a genetic insulator that can effectively isolate a transgene from positional effects of neighboring gene activities in transgenic plant cells.
Methods To Identify Plant Metabolites, Deane L. Falcone, John M. Littleton
Methods To Identify Plant Metabolites, Deane L. Falcone, John M. Littleton
Plant and Soil Sciences Faculty Patents
This invention provides materials and methods to manipulate the plant genome at the level of single plant cells in culture resulting in the ability to assign metabolic functionality to plant genes involved in the production of biologically active molecules and to create a means of product discovery based on the biosynthetic capacity of plants. The materials to create an activation mutagenesis include incorporation of enhancer sequences from a plant viral promoter at random places in the plant genome via Agrobacterium mediated DNA transfer (T-DNA). The usefulness is that genes in the immediate vicinity of the incorporation were activated which allows …
Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Crop Plants, George J. Wagner, Ryan W. Shepard
Utility Of Phylloplanins As Antibiotics, Selective Fungicides And For Enhancing Microbial Resistance In Crop Plants, George J. Wagner, Ryan W. Shepard
Plant and Soil Sciences Faculty Patents
The invention is directed to methods and compositions that inhibit pathogen proliferation. More particularly, these methods and compositions involve the use of phylloplane proteins as antibiotics against human and animal pathogens.