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The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey Nov 2022

The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey

Masters Theses

The ability of plants to absorb light through photoreceptors is essential for successful plant development. Phytochromes are important plant photoreceptors that detect and absorb red and far-red light and must function properly for plant survival. The Light Response BTB proteins (LRB) play an important role in a plant’s response to red light by degrading phytochrome B (PhyB) via the ubiquitin proteasome system (UPS). Although there has been a successful investigation into understanding the function of the LRBs, the function of a few regions within LRB proteins remains unknown, including a portion located on the N-terminal end of the protein, termed …


Assessing Warm-Season Annual Grasses To Increase Forage Inventory, Andrea Marroquin Oct 2022

Assessing Warm-Season Annual Grasses To Increase Forage Inventory, Andrea Marroquin

Masters Theses

Summers are expected to continue to increase in heat/dryness in the Northeast, causing issues pertaining to forage production during the summer to worsen. Many pastures grow cool season grasses, even during the summer. These grasses enter a dormant period and slowdown in production during the months of July and August, leading to what is referred to as “summer slump”. Some farms grow corn silage during the summer, and while corn silage is a valuable crop, its cultivation often does not support soil biology. This research addresses solutions for both summer slump foraging and more sustainable silage. Summer annuals grow more …


Evaluation Of Semiochemicals For Attractiveness To Multiple Tortricid (Lepidoptera) Pests In Apple Orchards, Ajay P. Giri Oct 2022

Evaluation Of Semiochemicals For Attractiveness To Multiple Tortricid (Lepidoptera) Pests In Apple Orchards, Ajay P. Giri

Masters Theses

Tortricid moths (Lepidoptera) are known for their outstanding olfaction capabilities that allow them to detect, process, and respond to chemical information emitted by host or non-host plants. Such an ability to filter out odors from the complex mixture to locate their host has allowed researchers and integrated pest management (IPM) practitioners to develop and implement semiochemically-based pest control strategies. The major goal of this study was to evaluate, under field conditions, the response of male and female oriental fruit moth (OFM), codling moth (CM), redbanded leafroller (RBLR) and three lined leafroller (TLLR) to experimental kairomone lures in commercial apple orchards …


Evaluating Current And Future Potential Distribution Of Epiphytic Orchids In The Congo Basin With Ecological Niche Models, Michael L. Ngoh Aug 2022

Evaluating Current And Future Potential Distribution Of Epiphytic Orchids In The Congo Basin With Ecological Niche Models, Michael L. Ngoh

Masters Theses

The Congo Basin Forest harbors a rich diversity of epiphytic communities, with the Orchidaceae alone making up more than 50% of all epiphytes in the region. Despite the huge diversity of epiphytes, many species, including epiphytic orchids, are at risk to a diverse array of threats. Climate change for instance poses severe threats to epiphytic orchids due to elevated temperatures, prolonged periods of droughts, as well as reduced rainfall across the Congo Basin Forest. In this study, we used ecological niche modeling and GIS techniques to identify spatial patterns of species richness, potential future climate refugia, and novel climatic suitability …


Diterpene Synthases Of The Rice Blast Fungus: Phylogenetic Analysis And Biochemical Characterization, Ayousha Shahi May 2022

Diterpene Synthases Of The Rice Blast Fungus: Phylogenetic Analysis And Biochemical Characterization, Ayousha Shahi

Masters Theses

Plant-pathogenic fungi harbor various specialized metabolites including diterpenoids that function as hormones and virulence factors. The fungus Magnaporthe oryzae is the causal agent of rice blast disease and can infect over 50 grass species. We demonstrate that the rice blast fungus encodes two diterpene synthases that produce normal pimara-8,15-diene and manoyl oxide scaffolds. Phylogenetic analysis of diterpene synthases among rice blast pathotypes showed functional conservation of the two core diterpene synthases amongst all pathotypes and suggests further expansion in select grass species. These insights into the blast fungal terpenome may inform efforts to counteract deleterious phytopathogens in crucial food crops.


The Influence Of Cover Crop Termination Strategies And Supplemental Nitrogen On Sweet Corn Yield And Nitrogen Use Efficiency, Sachina Sunuwar May 2022

The Influence Of Cover Crop Termination Strategies And Supplemental Nitrogen On Sweet Corn Yield And Nitrogen Use Efficiency, Sachina Sunuwar

Masters Theses

Cover crops are instrumental in nutrient management and soil health. A field experiment was conducted from fall 2020 to summer 2021 to evaluate the efficiency of different cover crop termination techniques and supplemental nitrogen on sweet corn yield, ear quality and nitrogen use efficiency. The four termination types are herbicide and disk tillage (HDT), herbicide no-till (HNT), cultivation disk till (CDT), and roller crimped no-till (RCNT). These termination types were each divided into four different nitrogen treatment: 0%, 33%, 67% and 100% of the recommended doses (145 kg haˉ¹). Sweet corn yield was highest and % unfilled tip was lowest …


A Bioassay To Determine Poa Annua Responses To Indaziflam, Benjamin D. Pritchard May 2022

A Bioassay To Determine Poa Annua Responses To Indaziflam, Benjamin D. Pritchard

Masters Theses

Herbicide resistance within Poa annua is widespread in managed turfgrass systems. In 2020, a P. annua collection from a golf course in the southeastern United States was reported to be resistant to indaziflam as well as six other mode-of-action groups. Considering P. annua is the most troublesome weed in turfgrass, a bioassay to screen other collections with putative indaziflam resistance is needed. A dose-response experiment was conducted with ten concentrations of indaziflam (0, 250, 500, 750, 1000, 1250, 1500, 2000, 4500, and 9000 pM) in Gelrite® culture during 2021 and 2022. An herbicide-susceptible (S1) collection of P. annua, a …


Improving Water And Plant Growth Regulator (Pgr) Management Practices In Cotton: Cultivar Physiological Responses And Pgr Rates, Kurt Wedegaertner May 2022

Improving Water And Plant Growth Regulator (Pgr) Management Practices In Cotton: Cultivar Physiological Responses And Pgr Rates, Kurt Wedegaertner

Masters Theses

Cotton (Gossypium hirsutum L.) is grown in an increasingly unpredictable climate and often under rainfed conditions. Without irrigation to alleviate common drought conditions, cultivar selection becomes a primary tool in mitigating the negative effects of water-deficit stress. Cultivars can differ in water use under water-limited environments, which can affect crop performance. Moreover, cotton management must involve balancing vegetative and reproductive growth through the use of plant growth regulators (PGR) due to the plant’s perennial growth pattern. The efficacy of PGRs are dependent on many variables including environmental conditions, irrigation, and genetics (cultivar). In 2020 and 2021, a series of …


Songbird-Mediated Insect Pest Control In Low Intensity New England Agriculture, Samuel J. Mayne Mar 2022

Songbird-Mediated Insect Pest Control In Low Intensity New England Agriculture, Samuel J. Mayne

Masters Theses

Global agricultural intensification has caused large-scale wildlife declines, but agricultural lands that maintain natural habitats can support healthy wildlife populations and receive significant ecosystem services from these natural communities. However, how on-farm biodiversity results in beneficial ecosystem services is highly variable and is reported to differ among taxa and guilds. One group that has attracted attention for their potential beneficial role in reducing pest abundance are birds. Understanding the role of bird communities and individual species in pest control could be important for managing farms under a low intensity agroecological framework. In New England, farmers are increasingly applying low intensity …


Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage Mar 2022

Screening For Binding Partners And Protein-Protein Interactions Of A Fungal Transcription Factor- Xdr1, Nishadi Punsara Gallala Gamage

Masters Theses

Clarireedia spp. (formerly Sclerotinia homoeocarpaF.T. Bennett) is the causal agent dollar spot, the most economically important turfgrass disease impacting golf courses in North America. The most effective strategy for dollar spot control is repeated application of multiple classes of fungicides. However, reliance on chemical application has led to resistance to four classes of fungicides as well as multidrug resistance (MDR). Fungi are known to detoxify xenobiotics, like fungicides, through transcriptional regulation of three detoxification phases: modification, conjugation and secretion. Little is known, however, of the protein-protein interactions that facilitate these pathways. Following next-generation RNA sequencing of Clarireedia spp., a …