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Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden
Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden
Honors Theses
Chestnut blight is a disease caused by the ascomycete fungus Cryphonectria parasitica in the Castanea species. The fungus uses oxalic acid (OA) to attack the tree’s cells. Castanea dentata, the American chestnut, was wiped out by chestnut blight in the early to mid-20th century, but several East Asian Castanea species appear highly resistant to the fungus. To breed resistant American type trees, screening methods are used to enable selection of interspecific hybrids. The alternative small stem assay (aSSA) is a method of screening container-grown hybrid seedlings during their first growing season by directly infecting them with C. parasitica. Some plant …