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Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden
Oxalic Acid Leaf Disk Soak Assay Is A New Possibility In Screening For Blight Resistance In Castanea Species, Kaitlyn Harden
Honors Theses
Chestnut blight is a disease caused by the ascomycete fungus Cryphonectria parasitica in the Castanea species. The fungus uses oxalic acid (OA) to attack the tree’s cells. Castanea dentata, the American chestnut, was wiped out by chestnut blight in the early to mid-20th century, but several East Asian Castanea species appear highly resistant to the fungus. To breed resistant American type trees, screening methods are used to enable selection of interspecific hybrids. The alternative small stem assay (aSSA) is a method of screening container-grown hybrid seedlings during their first growing season by directly infecting them with C. parasitica. Some plant …
A Small Stem Assay Using Hypovirulent Cryphonectria Parasitica To Screen Castanea Dentata Backcross F2 Families May Set The Stage For Long-Term Survival, Trish Nguyen
Honors Theses
By combining the results of blight resistance breeding and the application of hypovirulence as a biocontrol, populations of resistant hybrid trees could be deployed together with a less pathogenic strain of Cryphonectria parasitica (Murr.) Barr. I used a small stem assay to screen seedlings in thirteen half-sibling backcross F2 families with an attenuated strain of C. parasitica containing the Cryphonectria parasitica hypovirus-1 (CHV-1) Euro7 virus. The experiment was set up as a randomized complete block design in 2-gallon containers. Measurements of canker length and morphology were gathered at 90 days post-inoculation. Although statistically significant differences were seen between canker lengths …