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Gene Expression And Physiological Analyses To Study Metabolic Shifts In Medicago Truncatula Subjected To Biotic Stresses, Michelle Ryan Martin
Gene Expression And Physiological Analyses To Study Metabolic Shifts In Medicago Truncatula Subjected To Biotic Stresses, Michelle Ryan Martin
Graduate Theses and Dissertations
Plants are frequently under attack from pests and deploy various defense mechanisms to fight off predators. Many plant defenses are induced following herbivory or pathogen infection. An investment in defense could potentially lead to a diversion of metabolic resources away from primary metabolism, including reduction in plant commitment to photosynthesis. In this thesis, changes in expression levels of representative genes for primary and secondary metabolism were studied using real-time polymerase chain reaction (PCR) in Medicago truncatula Gaertner subjected to beet armyworm (Spodoptera exigua H¨¹bner) damage or Phoma medicaginis Malbr. & Roum. infection. Photosynthetic yield was also measured using chlorophyll fluorescence …