Digital Commons Network^™

Multi-Tactic Ecological Weed Management In A Changing Climate, Sonja K. Birthisel

Electronic Theses and Dissertations

Climate change is expected to impact weed communities in Maine, and the efficacy of tools and tactics farmers use to manage them. Through seedbank sampling and surveys of Maine organic farms, we identified currently rare weeds that are known to be especially abundant or problematic in warmer areas of the USA and might therefore represent an emerging agronomic risk. Many ecological weed management strategies that focus on depleting the weed seedbank are expected to remain effective in a changing climate, and become increasingly important as efficacy of cultivation and some herbicide applications diminish or become more variable. Through field experiments, …

Establishment Of Turfgrass From Seed And Sod In Sand Based Systems Combining Sprinkler And Subsurface Drip Irrigation Systems, Roshaan Ahmed Khan Niazi

Electronic Theses and Dissertations

The establishment of turfgrass using subsurface drip irrigation (SDI) in sand based media has limited success. However, it is unknown if starting with overhead (sprinkler) irrigation and transitioning to SDI can enhance turfgrass establishment. Two experiments were conducted at Stephen F. Austin State University (SFASU) to investigate the establishment of “L-93” creeping bentgrass (Agrostis stolonifera) from seed and “TifEagle” ultra dwarf hybrid bermudagrass (Cynodon dactylon x Cynodon transvaalensis) from sod using sand based media. Each experiment consisted of two trials conducted in summer 2017 and spring 2018. Both creeping bentgrass trials and the spring ultra dwarf …

Climatic Range Filling Of North American Trees, Benjamin Seliger

Electronic Theses and Dissertations

Understanding the degree to which species distributions are controlled by climate is crucial for forecasting biodiversity responses to climate change. Climatic equilibrium, when species are found in all places which are climatically suitable, is a fundamental assumption of species distribution models, but there is evidence in support of climate disequilibria in species ranges. Long-lived, sessile organisms such as trees may be especially vulnerable to being outpaced by climate change, and thus prone to disequilibrium. In this dissertation, I tested the degree to which North American trees are in equilibrium with their potential climatic ranges using the ‘range filling’ metric, which …

Characterization Of Acyltransferases And Wrinkled Orthologs Involved In Tag Biosynthesis In Avocado, Md Mahbubur Rahman

Electronic Theses and Dissertations

Triacylglycerols (TAG) or storage oils in plants are utilized by humans for nutrition, production of biomaterials and fuels. Since nonseed tissues comprise the bulk biomass, it is pertinent to understand how to improve their TAG content. Typically, the final step in TAG biosynthesis is catalyzed by diacylglycerol (DAG) acyltransferases (DGAT) and/or phospholipid: diacylglycerol acyltransferases (PDAT), which also determine the content and composition of TAG. Besides enzymatic regulation of TAG synthesis, transcription factors such as WRINKLED1 (WRI1) play a critical role during fatty acid synthesis. In this study, mesocarp of Persea americana, with > 60% TAG by dry weight and oleic …

Localization Of Sip470, A Plant Lipid Transfer Protein In Nicotiana Tabacum, Shantaya Andrews

Electronic Theses and Dissertations

SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed …

Bryophyte Assemblages On American Hornbeam (Carpinus Caroliniana) And The Surrounding Arboreal Communities Within The Forest Of East Texas, Cassey Edwards

Electronic Theses and Dissertations

The bryophytes, the non-vascular plants, include the mosses (Bryophyta with approximately 13,000 species), the liverworts (Marchantiophyta with approximately 5,000 species), and the hornworts (Anthocerotophyta with approximately 150 species). Initial observations indicated that the midstory tree Carpinus caroliniana Walter (American hornbeam) has a bryophyte composition with an unusually high diversity of leafy liverworts. Voucher samples were taken from the four intercardinal directions (Northeast, Northwest, Southwest, and Southeast) from six Carpinus trees and their nearest neighbor that was neither Pinus nor another Carpinus at each of six sites across East Texas. Percent coverage, species richness, Shannon’s diversity and Pielou’s evenness were computed. …

Sip-428, A Sir2 Deacetylase Enzyme And Its Role In Biotic Stress Signaling Pathway, Bal Krishna Chand Thakuri

Electronic Theses and Dissertations

SABP2 (Salicylic Acid Binding Protein 2) plays a vital role in the salicylic acid signaling pathway of plants both regarding basal resistance and systemic acquired resistance against pathogen infection. SIP-428 (SABP2 Interacting Protein-428) is a Silent information regulator 2 (SIR2) like deacetylase enzyme that physically interacts with SABP2 in a yeast two-hybrid interaction and confirmed independently by a GST pull-down assay. We demonstrated that SIP- 428 is an NAD+ dependent SIR2 deacetylase enzyme. Transgenic tobacco plants silenced in SIP- 428 expression via RNAi showed enhanced basal resistance to microbial pathogens. Moreover, these SIP-428-silenced lines also exhibited a robust induction of …

Breeding Bird Response To Post Oak Savanna Restoration Seven Years Post Management In Eastern Texas, Courtney Mcinnerney

Electronic Theses and Dissertations

Oak savannas were once an abundant vegetation type in the Midwestern United States that have now declined to <1% of their original distribution. Historically, natural disturbances such as periodic fire and grazing maintained oak savannas, but these have been reduced or eliminated, resulting in woody encroachment and subsequent habitat loss and degradation. In 2009-10, a baseline, pre-restoration study was completed to determine vegetation characteristics, breeding bird abundances, nest success, and nest site selection at the Gus Engeling Wildlife Management Area (GEWMA) in eastern Texas. The results showed a lack of savanna vegetation structure on degraded sites and few savanna or grassland obligate bird species. The goal of this study was to determine how breeding birds of oak savanna vegetation types in eastern Texas respond to restoration effects 7 years after initial management. Post-restoration surveys completed in 2016-17 showed a change in avian assemblages from a more woodland dominated community to grassland/savanna community. The presence and breeding of savanna obligate species dickcissel (Spiza americana) and lark sparrow (Chondestes grammacus) indicates that the restoration was successful. The presence of savanna species can be linked to the herbaceous vegetation that was restored to more closely resemble historic oak savanna structure and can quantify the success of restoration efforts.

Flower Visitation In Relation To Pollen And Nectar Nutrition: Implications For Pollinator Habitat And Conservation, Megan E. Leach

Electronic Theses and Dissertations

Pollination of both wild and crop plants is at a crossroads; honey bee populations are experiencing losses at a higher rate than ever before, and some native bee species are declining in abundance to the point of being listed as endangered species. A few examples of these threats include pesticide exposure, habitat loss, and climate change. In response to bee population declines, conservation efforts have been initiated to increase habitat quality for bees by planting pollinator reservoirs or gardens. Plants provide nutrition to bees in the form of pollen and nectar. Several studies have shown links between higher nutritional quality …

Factors Affecting Survival And Carpogenic Germination Of Pseudosclerotia Of Monilinia Vaccinii-Corymbosi, The Causal Agent Of Mummy Berry, On Vaccinium Angustifolium In Maine, Tyler L. Case

Electronic Theses and Dissertations

The primary focus of this research project was to identify factors in the field affecting survival and the environmental conditions affecting carpogenic germination of pseudosclerotia of Monilinia vaccinii-corymbosi (MVC) which is a common fungal pathogen of lowbush blueberry (Vaccinium angustifolium) in Maine. The interactions between biological oranisms, such as fungi and insects with pseudosclerotia prior to and during the overwintering process were investigated in the research outlined below. Fungi from the surfaces of pseudosclerotia were screened as potential antagonists in a co-plate experiment. Most of the potentially antagonistic fungi tested reduced the width of the MVC reference cultures. These potential …

Consumer Acceptance And Phytonutrient Assessment Of Cold Hardy, Locally Grown Plums, Amber L. Elwell

Electronic Theses and Dissertations

Prunus salicina, Japanese plums, and Prunus domestica, European plums, are naturally rich in fiber, minerals, vitamins and phytonutrients. Consuming plums can increase overall fruit intake and can be incorporated into part of a healthy diet. Through local plum production, harvesting can be completed without the need for cross-country shipping, which can increase overall acceptability and nutritional quality of the fruit. Furthermore, the increase in local production can help to boost Maine’s economy and creates a market for new plum product production.

The first objective of this study was to determine consumer acceptance of locally grown plum cultivars at a …

Propagation Of The Native North American Shrub Lonicera Villosa And Trait Comparisons With Nonnative Congeneric Taxa, Darren J. Hayes

Electronic Theses and Dissertations

The honeysuckles, or Lonicera, represent a circumboreally-distributed genus in the Caprifoliaceae family. A diverse assortment of vines, lianas, and shrubs comprise the genus, and honeysuckles have long been a staple of the horticultural industry, prized for their robustness as much as their sweet-scented flowers and colorful fruits. However, many cultivated honeysuckles of Eurasian origin have proven invasive outside of their native range, displacing native species and reducing overall diversity and ecosystem health. Planting of Eurasian Lonicera taxa is now often discouraged or banned throughout much of North America. Conversely, native North American Lonicera species are often sparsely distributed and …

Band-Sowing With Hoeing For Weed Management In Organic Grains, Margaret R. Mccollough

Electronic Theses and Dissertations

Weeds remain the foremost production challenge for organic small grain growers in the Northeastern United States. Instead of sowing crops in narrow, single-line rows, band-sowing with inter-band hoeing is a cropping strategy that could provide superior weed control. In theory, band-sowing suppresses weeds in the intra-band zone by improving the spatial arrangement of the crop from that of typical rows to a more uniform pattern within the planted bands, maximizing interspecific and reducing intraspecific competition. Weeds in the inter-band zone are controlled by cultivating with aggressive sweeps; tine harrowing can target weeds in the intra- and inter-band zones.

Chapter one …

Effect Of Pinching And Spacing On The Growth And Development Of Sunflowers (Helianthus Annuus) In East Texas, Bilawal Irshad Cheema

Electronic Theses and Dissertations

Helianthus annuus cultivars were grown in East Texas to evaluate the effect of pinching and spacing on their growth and development. The first experiment was conducted twice (Trial 1A, 28 April 2017 and Trial 1B, 3 August 2017) to evaluate the effect of pinching at nodes 1, 2, 3, and 4 on sunflower cultivars ‘Superior Gold’, ‘Pro Cut Gold’, ‘Sun Bright Supreme’, ‘Vincent’s Choice’, and ‘Sunrich Lemon’. For trial 1A, all the non-pinched treatments for the five cultivars produced marketable stem lengths, stem diameters, flower diameters, and disk diameters. For the cultivar ‘Superior Gold’ all the treatments produced marketable stems. …

Optimizing Fertilizer And Compost Rates In Organic Reduced Till Agriculture, Nicholas W. Rowley

Electronic Theses and Dissertations

Interest in reduced tillage systems has been increasing and advanced by a need to practice agriculture in a sustainable way to limit environmental degradation. The focus on preserving soil integrity has become commonplace in all scales of agriculture. Recently trends in agricultural production have given rise to numerous small farms centered on local and sustainable farming. In turn, this has led to a growing desire to implement conservation tillage into these systems.

In 2015 and 2016 a collaborative effort between the University of Maine at the Maine Agricultural and Forestry Experiment Station: Highmoor Farm and Cornell University at the Homer …

Reducing Tillage In Small-Scale Permanent Bed Organic Vegetable Production Systems, Jeremiah D. Vallotton

Electronic Theses and Dissertations

The response of field-grown vegetable crops to reduced tillage and mulching in permanent beds was evaluated through measuring crop yields, weed pressure, earthworm counts, and soil basal respiration. Two vegetable crops (“Bush Delicata” squash and “Farao” cabbage) were started in April and May of 2016 and 2017 respectively, transplanted in late June, and harvested on 15-Sep-2016 and 25-Aug-2017. Fruit number and weight of squash, and head weight and feeding damage of cabbage were measured. These results suggest that intensive tillage (8” rototill every year) can be successfully reduced to alternating years of shallow (2”) rototilling and a less intensive form …

Soil Ecology Of The Exotic Dune Grass Leymus Arenarius., Matthew L. Reid

Electronic Theses and Dissertations

Exotic plants can alter the structure and function of native communities both above- and belowground, which is particularly important in primary successional sand dune ecosystems where plant-soil interactions are critical drivers of successional dynamics. In Great Lakes sand dunes, the native foundation grass Ammophila breviligulata dominates early in succession until an accumulation of detrimental soil organisms causes its die-back of the grasses. Die-back of this native foundation species can be slowed by mutualistic interactions between plants and mycorrhizal fungi. Changes to the plant community resulting from invasion, and subsequent effects on the soil community have the potential to disrupt the …

Big Data For Small Parks: Examining Regional Vegetation Patterns To Assess The Current Condition And Vulnerability Of Eastern National Parks To Climate Change, Kathryn M. Miller

Electronic Theses and Dissertations

The United States National Park Service mission is to preserve natural and cultural resources unimpaired for future generations. Given climate change, the paradigm of restoring natural resources to their pre-European settlement condition is no longer appropriate or achievable management. Instead, we must promote resilience and plan for adaptation. This approach poses many challenges, including knowledge gaps about the current condition of park ecosystems including wetlands, and lack of information about the matrix surrounding parks, which will strongly influence park ecosystem response to climate change. My dissertation research focused on filling these knowledge gaps to provide much needed information to managers …

Modeling Soil Organic Carbon In Select Soils Of Southeastern South Dakota, Shaina Westhoff

Electronic Theses and Dissertations

Soil organic matter (SOM) is composed of living biomass, dead plant and animal residues, and humus. Humus is a class of complex, organic molecules that are largely responsible for improving soil water holding capacity, nutrient mineralization, nutrient storage, and other critical soil functions. Soil organic carbon (SOC) accounts for approximately 60 percent of SOM and thus SOC is recognized as a strong indicator of soil health. Land use changes and intense cultivation of arable soils in the United States over the past century have led to large decreases in SOM. The objective of this research was to develop a multiple …

Impacts Of Diverse Crop Rotations And Integrated Crop-Livestock System On Soil Quality In South Dakota, Atilla Polat

Electronic Theses and Dissertations

Crop diversification can be beneficial in improving soil quality parameters. Diverse crop rotations and integrated crop-livestock systems (ICLS), are two examples of crop diversification used in this study to assess soil quality. The objectives of this study were to: (i) assess the impacts of diverse rotations on soil quality parameters, and (ii) assess the impacts of low and high stocking density on soil quality parameters. Both of the studies were conducted separately and are presented as two different chapters. To accomplish objective (i), a long-term experiment was selected which was established near Brookings, South Dakota, USA at USDA-ARS lab in …

Tripartite Interactions Of Legumes With Arbuscular Mycorrhizal Fungi And Rhizobial Bacteria: Insight Into Plant Growth, Seed Yield, And Resource Exchange, Arjun Kafle

Electronic Theses and Dissertations

Under natural conditions, legumes, such as alfalfa (Medicago) and soybean (Glycine max) are colonized with arbuscular mycorrhizal (AM) fungi and rhizobial bacteria forming tripartite interactions. Legumes are important crop species due to their high nutritional and economic values. Most of the previous literatures focused on experiments with an individual symbiont: either AM fungi or rhizobial bacteria, but not with both symbionts at the same time, thus our current understanding of resource exchange in tripartite interactions is limited. It has been reported that AM fungi primarily provide phosphate (P), nitrogen (N), and other nutritional and non-nutritional benefits while rhizobial bacteria solely …

Greenhouse Gas Emissions From Soil Applied Nitrogen Fertilizers, Samuel Thies

Electronic Theses and Dissertations

Greenhouse gases (GHG) absorb and emit radiant energy in the thermal range. Their concentrations in the atmosphere are increasing and becoming an ever increasing issue as the Earth’s average temperature continues to rise. Agricultural practices, such as applying nitrogen fertilizer to increase crop yield can be a major source of GHG emissions and methods are needed to decrease these emissions. The measurements of GHG emissions have been difficult, expensive, and erratic. However, advancing technologies have increased the accuracy and confidence in measuring GHG emissions. The objectives of this study were to determine the 1) effects of different nitrogen fertilizer sources, …

Soybean Iron Deficiency Chlorosis: Quantitative Trait Locus Validation And Grain Yield Evaluation, Ahmed Charif

Electronic Theses and Dissertations

Soybean production in most of the Northern Great Plain area has been challenged by iron deficiency chlorosis (IDC), which is a physiological problem with a plant grown in high pH, calcareous soil. Developing IDC-resistant cultivars is the best approach to meet this challenge. Currently, this approach is limited by lack of knowledge about genetic resources and mechanisms for resistance to IDC. The objectives of this research were to validate quantitative trait loci (QTL) associated with IDC and to evaluate the effect of IDC on yield in soybean cultivars. To validate the QTL, a population of 201 recombinant inbred lines, which …

Expression Analyses And Identification Of Key Molecular Participants In Plant Responses To Environmental Cues, Praveena Kanchupati

Electronic Theses and Dissertations

Plants constantly engage and interact with the environment and respond to the changes in conditions like temperature, water, and photoperiod, by regulating expression of genes of multiple regulatory and signaling pathways. Insight into these pathways and their participants has provided and will provide candidates to improve various agronomically important traits in crops through marker-assisted breeding and genetic manipulation. With this aim in mind, in the present study, I attempted to identify key candidate genes that are involved in the regulation of; i) plant response to low temperature stress, ii) plant roots’ response to soil moisture content and iii) flowering time. …

Regulation Of Local Auxin Metabolism During Soybean Nodule Development, Suresh Damodaran

Electronic Theses and Dissertations

Legume-rhizobia symbiosis leads to the development of secondary root organs called nodules. Rhizobia bacteria housed inside nodules assimilate atmospheric nitrogen and convert them into plant usable forms thereby reducing the need for fertilizer application in crop legumes like soybean. Nodule development is a coordinated process orchestrated by multiple plant hormones. In soybean, the auxin responsive gene expression was detected in nodule primordia and in the periphery of mature nodules, primarily in nodule vasculature. Auxin hypersensitivity reduces nodule formation in soybean and also polar auxin transport inhibition at the site of nodule development is not crucial for determinate nodule formation. Therefore, …

Quantifying The Short-Term Impacts Of Cover Crops And Grazing On Soil Health Under An Integrated Crop-Livestock System In South Dakota, Vishal Seth

Electronic Theses and Dissertations

Cover crops and grazing under Integrated Crop-Livestock System (ICLS) can impact the rhizosphere nutrient cycling and may have potential to increase the long-term sustainability and economic profitability of agricultural production system. In South Dakota, crop production practices and livestock husbandry are both common which provide the opportunity for ICLS to be successful in this region. However, little is known about the impacts of the cover crops and grazing under ICLS on soil properties in this region. The present study was conducted at South Dakota State University Research Farm (44°20'34.8"N, 96°48'14.8"W), Brookings, SD, to quantify the impacts of cover crops and …

Insecticidal Seed And In-Furrow Treatment Recommendations For Soybean And Sunflower, Brady Hauswedell

Electronic Theses and Dissertations

Throughout the 2016 and 2017 growing season, field research experiments were replicated across South Dakota. Many times seed treatments are used prophylactic, which is neither good for the producers or the environment. Producers will be able to reduce production costs, if they only use a seed treatment when necessary. The purpose of the first experiment was to determine the effects of seed treatments in combination with planting date and seeding rate on soybean yield. To determine the effects, two years of field data from four eastern South Dakota locations were compared. Within each year and location we compared two planting …

Map-Based Cloning And Molecular Characterization Of The Seed Dormancy 10 Locus In Rice (Oryza Sativa L.), Wirat Pipatpongpinyo

Electronic Theses and Dissertations

Seed dormancy is a key adaptive trait of both ecological and agricultural importance. Although many quantitative trait loci (QTL) have been associated with seed dormancy in cereal crops or model plants, a majority of them remain unknown for molecular identities and functions. Cereal crops and wild/weedy relatives usually have weak and strong seed dormancy, respectively, such as Asian cultivated rice vs. weedy rice (Oryza sativa L.). Previous research identified a set of seed dormancy QTL, including qSD10, in single plant-derived BC₁F₂ and BC₁F₃ populations from the backcross between the weedy rice line SS18-2 …

Assessment And Development Of Cold Temperature Germination Tolerance In Oat And Hard Red Spring Wheat, Jacob Baustian

Electronic Theses and Dissertations

Oat (Avena Sativa) and hard red spring wheat (HRSW; Triticum aestivum L.) are both spring planted cereal crops that yield best when planted early in the spring. These crops would benefit from improvements in cold temperature germination tolerance (CTGT) by allowing them faster and more uniform germination and emergence early in the spring. This study aimed to (i) evaluate germination in oat genotypes over a range of sub-optimal temperatures and (ii) establish a methodology for selection of hard red spring wheat for CTGT. Seed samples of ten different oat genotypes were evaluated for CTGT in a germination chamber …

Soybean Aphid Biotype 4 Resistance In Soja And Soybean Plant Introductions, Sophia R. Conzemius

Electronic Theses and Dissertations

Soybean aphid, Aphis glycines Matsumura, is a major pest to soybean, Glycine max (L.) Merr. Host plant resistance is a management tactic that uses naturally occurring soybean plant defenses to limit soybean aphid pest damage. Virulent soybean aphid biotypes are able to successfully colonize on certain aphid resistant soybean. Soybean aphid biotype 4 is most virulent, overcoming all commercially available soybean aphid resistant soybeans (Rag1, Rag2, and Rag1+Rag2). Additional sources of resistance to avirulent biotypes have been identified in soja and soybean plant introductions (PIs). This study examined those resistant soja and soybean for resistance to the newly found soybean …