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Plant Organellar Protein Targeting: A Traffic Plan Still Under Construction, Sally Ann Mackenzie
Plant Organellar Protein Targeting: A Traffic Plan Still Under Construction, Sally Ann Mackenzie
Center for Plant Science Innovation: Faculty and Staff Publications
It has long been understood that specific features of a protein and its corresponding import apparatus dictate the behavior of mitochondrial proteins in their intracellular targeting behavior. In plants, the process by which proteins are directed to organelles has been influenced uniquely by the introduction to the cell of plastids. Parallel functions carried out within the mitochondrion and plastid permit the sharing of proteins and emergence of mechanisms to facilitate dual-targeting of the nuclear-encoded products to both compartments. These include transcriptional and translational variations, relaxation of translation initiation controls and conditional cellular influences. Details of the dual targeting system are …
Pseudomonas Syringae Type Iii Chaperones Shco1, Shcs1, And Shcs2 Facilitate Translocation Of Their Cognate Effectors And Can Substitute For Each Other In The Secretion Of Hopo1-1, Ming Guo, Scott T. Chancey, Fang Tian, Zhengxiang Ge, Yashitola Jamir, James R. Alfano
Pseudomonas Syringae Type Iii Chaperones Shco1, Shcs1, And Shcs2 Facilitate Translocation Of Their Cognate Effectors And Can Substitute For Each Other In The Secretion Of Hopo1-1, Ming Guo, Scott T. Chancey, Fang Tian, Zhengxiang Ge, Yashitola Jamir, James R. Alfano
Center for Plant Science Innovation: Faculty and Staff Publications
The Pseudomonas syringae type III secretion system (TTSS) translocates effector proteins into plant cells. Several P. syringae effectors require accessory proteins called type III chaperones (TTCs) to be secreted via the TTSS. We characterized the hopO1-1, hopS1, and hopS2 operons in P. syringae pv. tomato DC3000; these operons encode three homologous TTCs, ShcO1, ShcS1, and ShcS2. ShcO1, ShcS1, and ShcS2 facilitated the type III secretion and/or translocation of their cognate effectors HopO1-1, HopS1, and HopS2, respectively. ShcO1 and HopO1-1 interacted with each other in yeast two-hybrid and coimmunoprecipitation assays. Interestingly, ShcS1 and ShcS2 were capable of substituting for …
United States Patent Application Publication: Maize Chloroplast Protein Synthesis Elongation Factors And Methods Of Use For Same, Zoran Ristic, Shailendra K. Bhadula, Genping Yang, Thomas Elthon, Jeffrey E. Habben
United States Patent Application Publication: Maize Chloroplast Protein Synthesis Elongation Factors And Methods Of Use For Same, Zoran Ristic, Shailendra K. Bhadula, Genping Yang, Thomas Elthon, Jeffrey E. Habben
Center for Plant Science Innovation: Faculty and Staff Publications
The invention discloses a novel heat shock protein with high homology to chloroplast elongation factor EF-Tu. Also disclosed is a transgenic method for enhancing tolerance to heat and drought in female reproductive organs. It involves the temporal and spatial expression of novel heat shock EF-Tu in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase heat and drought tolerance.
Functional Specialization Of Chlamydomonas Reinhardtii Cytosolic Thioredoxin H1 In The Response To Alkylation-Induced Dna Damage, Nandita Sarkar, Stephane Lemaire, Danxia Wu-Scharf, Emmanuelle Issakidis-Bourguet, Heriberto D. Cerutti
Functional Specialization Of Chlamydomonas Reinhardtii Cytosolic Thioredoxin H1 In The Response To Alkylation-Induced Dna Damage, Nandita Sarkar, Stephane Lemaire, Danxia Wu-Scharf, Emmanuelle Issakidis-Bourguet, Heriberto D. Cerutti
Center for Plant Science Innovation: Faculty and Staff Publications
DNA damage occurs as a by-product of intrinsic cellular processes, like DNA replication, or as a consequence of exposure to genotoxic agents. Organisms have evolved multiple mechanisms to avoid, tolerate, or repair DNA lesions. To gain insight into these processes, we have isolated mutants hypersensitive to DNA damaging agents in the green alga Chlamydomonas reinhardtii. One mutant, Ble-1, showed decreased survival when it was treated with methyl methanesulfonate (MMS), bleomycin, or hydrogen peroxide (H2O2) but behaved like the wild type when it was exposed to UVC irradiation. Ble-1 carries an extensive chromosomal deletion that includes …
Folate Synthesis And Metabolism In Plants And Prospects For Biofortification, Gilles J. C. Basset, Eoin P. Quinlivan, Jesse F. Gregory Iii, Andrew D. Hanson
Folate Synthesis And Metabolism In Plants And Prospects For Biofortification, Gilles J. C. Basset, Eoin P. Quinlivan, Jesse F. Gregory Iii, Andrew D. Hanson
Center for Plant Science Innovation: Faculty and Staff Publications
Folates are essential cofactors for one-carbon transfer reactions in most living organisms and are required for the biosynthesis of nucleic acids, amino acids, and pantothenate. Unlike plants and microorganisms, humans cannot synthesize folates de novo and must acquire them from the diet, primarily from plant foods. However, lack of folates is the most common vitamin deficiency in the world and has serious health consequences, including increased risk of neural tube defects in infants, cancers, and vascular diseases. Consequently, there is much interest in engineering plants with enhanced folate content (biofortification). In this review, we outline progress in defining the plant …
The Hrpk Operon Of Pseudomonas Syringae Pv. Tomato Dc3000 Encodes Two Proteins Secreted By The Type Iii (Hrp) Protein Secretion System: Hopb1 And Hrpk, A Putative Type Iii Translocator, Tanja Petnicki-Ocwieja, Karin V. Van Dijk, James R. Alfano
The Hrpk Operon Of Pseudomonas Syringae Pv. Tomato Dc3000 Encodes Two Proteins Secreted By The Type Iii (Hrp) Protein Secretion System: Hopb1 And Hrpk, A Putative Type Iii Translocator, Tanja Petnicki-Ocwieja, Karin V. Van Dijk, James R. Alfano
Center for Plant Science Innovation: Faculty and Staff Publications
Pseudomonas syringae is a gram-negative bacterial plant pathogen that is dependent on a type III protein secretion system (TTSS) and the effector proteins it translocates into plant cells for pathogenicity. The P. syringae TTSS is encoded by hrp-hrc genes that reside in a central region of a pathogenicity island (Pai). Flanking one side of this Pai is the exchangeable effector locus (EEL). We characterized the transcriptional expression of the open reading frames (ORFs) within the EEL of P. syringae pv. tomato DC3000. One of these ORFs, PSPTO1406 (hopB1) is expressed in the same transcriptional unit as hrpK. …