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Activation Of A Coi1-Dependent Pathway In Arabidopsis By Pseudomonas Syringae Type Iii Effectors And Coronatine, Ping He, Satya Chintamanani, Zhongying Chen, Lihuang Zhu, Barbara N. Kunkel, James R. Alfano, Xiaoyan Tang, Jian-Min Zhou
Activation Of A Coi1-Dependent Pathway In Arabidopsis By Pseudomonas Syringae Type Iii Effectors And Coronatine, Ping He, Satya Chintamanani, Zhongying Chen, Lihuang Zhu, Barbara N. Kunkel, James R. Alfano, Xiaoyan Tang, Jian-Min Zhou
Center for Plant Science Innovation: Faculty and Staff Publications
Gram-negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell-wall-degrading enzymes to promote parasitism in plants. However, little is known about how these virulence factors alter plant celluar responses to promote disease. In this study, we show that virulent Pseudomonas syringae strains activate the transcription of an Arabidopsis ethylene response factor (ERF) gene, RAP2.6, in a coronatine insensitive 1 (COI1)-dependent manner. A highly sensitive RAP2.6 promoter-firely luciferase (RAP2.6-LUC) reporter line was developed to monitor activities of various bacterial virulence genes. Analyses of P. syringae pv. tomato DC3000 mutants indicated …
Substoichiometric Shifting In The Plant Mitochondrial Genome Is Influenced By A Gene Homologous To Muts, Ricardo V. Abdelnoor, Ryan Yule, Annakaisa Elo, Alan C. Christensen, Gilbert Meyer-Gauen, Sally A. Mackenzie
Substoichiometric Shifting In The Plant Mitochondrial Genome Is Influenced By A Gene Homologous To Muts, Ricardo V. Abdelnoor, Ryan Yule, Annakaisa Elo, Alan C. Christensen, Gilbert Meyer-Gauen, Sally A. Mackenzie
Center for Plant Science Innovation: Faculty and Staff Publications
The plant mitochondrial genome is retained in a multipartite structure that arises by a process of repeat-mediated homologous recombination. Low-frequency ectopic recombination also occurs, often producing sequence chimeras, aberrant ORFs, and novel subgenomic DNA molecules. This genomic plasticity may distinguish the plant mitochondrion from mammalian and fungal types. In plants, relative copy number of recombination-derived subgenomic DNA molecules within mitochondria is controlled by nuclear genes, and a genomic shifting process can result in their differential copy number suppression to nearly undetectable levels. We have cloned a nuclear gene that regulates mitochondrial substoichiometric shifting in Arabidopsis. The CHM gene was …
United States Patent Application Publication: Maize Chloroplast Protein Synthesis Elongation Factors And Methods Of Use For Same, Zoran Ristic, Shailendra K. Bhadula, Genping Yang, Thomas Elthon, Jeffrey E. Habben
United States Patent Application Publication: Maize Chloroplast Protein Synthesis Elongation Factors And Methods Of Use For Same, Zoran Ristic, Shailendra K. Bhadula, Genping Yang, Thomas Elthon, Jeffrey E. Habben
Center for Plant Science Innovation: Faculty and Staff Publications
The invention discloses a novel heat shock protein with high homology to chloroplast elongation factor EF-Tu. Also disclosed is a transgenic method for enhancing tolerance to heat and drought in female reproductive organs. It involves the temporal and spatial expression of novel heat shock EF-Tu in a plant organ or plant tissue. The invention also includes expression constructs, and methods for the production of crop plants with heritable phenotypes which are useful in breeding programs designed to increase heat and drought tolerance.
Rna Interference: Traveling In The Cell And Gaining Functions?, Heriberto D. Cerutti
Rna Interference: Traveling In The Cell And Gaining Functions?, Heriberto D. Cerutti
Center for Plant Science Innovation: Faculty and Staff Publications
Double-stranded RNA can induce the degradation of homologous RNAs in organisms as diverse as protozoa, animals, plants and fungi, resulting in post-transcriptional gene silencing. But in some species, RNA-mediated processes can also lead to translational repression, DNA methylation, heterochromatin formation or DNA elimination. In some situations, amplification of the “trigger” double-stranded RNA seems to be required for efficient silencing. These findings imply that RNA-mediated mechanisms can control gene expression at both the transcriptional and the post-transcriptional level, and that they can operate in the nuclear and the cytoplasmic compartments.
United States Patent Application Publication: Isolation And Characterization Of Heat Shock Protein, Thomas Elthon, Adrian A. Lund, Dinakar Bhattramakki, David Rhoads
United States Patent Application Publication: Isolation And Characterization Of Heat Shock Protein, Thomas Elthon, Adrian A. Lund, Dinakar Bhattramakki, David Rhoads
Center for Plant Science Innovation: Faculty and Staff Publications
This invention relates to the identification and characterization of the maize HSP22 protein including the corresponding nucleic acid molecules, nucleic acid fragments and protein fragments. Further, this invention relates to both polyclonal and monoclonal antibodies to HSP22 and the production thereof. These antibodies can be used in a method for detecting the presence of HSP22 expression in whole leaf samples. Further, the nucleic acid molecule and fragments thereof can be used in assays to detect the levels of nucleic acid molecules involved in HSP22 expression. The levels of both HSP22 and HSP22 mRNA are then utilized as an indication of …
The Pseudomonas Syringae Type Iii-Secreted Protein Hopptod2 Possesses Protein Tyrosine Phosphatase Activity And Suppresses Programmed Cell Death In Plants, Avelina Espinosa, Ming Guo, Vincent C. Tam, Zheng Qing Fu, James R. Alfano
The Pseudomonas Syringae Type Iii-Secreted Protein Hopptod2 Possesses Protein Tyrosine Phosphatase Activity And Suppresses Programmed Cell Death In Plants, Avelina Espinosa, Ming Guo, Vincent C. Tam, Zheng Qing Fu, James R. Alfano
Center for Plant Science Innovation: Faculty and Staff Publications
The bacterial plant pathogen Pseudomonas syringae possesses a type III protein secretion system that delivers many virulence proteins into plant cells. A subset of these proteins (called Avr proteins) is recognized by the plant’s innate immune system and triggers defences. One defence-associated response is the hypersensitive response (HR), a programmed cell death (PCD) of plant tissue. We have previously identified HopPtoD2 as a type III secreted protein from P. s. pv. tomato DC3000. Sequence analysis revealed that an N-terminal domain shared homology with Avr- PphD and a C-terminal domain was similar to protein tyrosine phosphatases (PTPs). We demonstrated that purified …