Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Entire DC Network
A Cytoplasmic Male Sterility-Associated Mitochondrial Protein Causes Pollen Disruption In Transgenic Tobacco, Shichuan He, Andre R. Abad, Stanton B. Gelvin, Sally Ann Mackenzie
A Cytoplasmic Male Sterility-Associated Mitochondrial Protein Causes Pollen Disruption In Transgenic Tobacco, Shichuan He, Andre R. Abad, Stanton B. Gelvin, Sally Ann Mackenzie
Center for Plant Science Innovation: Faculty and Staff Publications
In higher plants, dominant mitochondrial mutations are associated with pollen sterility. This phenomenon is known as cytoplasmic male sterility (CMS). It is thought that the disruption in pollen development is a consequence of mitochondrial dysfunction. To provide definitive evidence that expression of an abnormal mitochondrial gene can interrupt pollen development, a CMS-associated mitochondrial DNA sequence from common bean, orf239, was introduced into the tobacco nuclear genome. Several transformants containing the orf239M gene constructs, with or without a mitochondrial targeting sequence, exhibited a semisterile or male-sterile phenotype. Expression of the gene fusions in transformed anthers was confirmed using RNA gel …
PhosphoEnolPyruvate Carboxylase: A Ubiquitous, Highly Regulated Enzyme In Plants, Raymond Chollet, Jean Vidal, Marion H. O'Leary
PhosphoEnolPyruvate Carboxylase: A Ubiquitous, Highly Regulated Enzyme In Plants, Raymond Chollet, Jean Vidal, Marion H. O'Leary
Center for Plant Science Innovation: Faculty and Staff Publications
Since plant phosphoenolpyruvate carboxylase (PEPC) was last reviewed in the Annual Review of Plant Physiology over a decade ago (O’Leary 1982), significant advances have been made in our knowledge of this oligomeric, cytosolic enzyme. This review highlights this exciting progress in plant PEPC research by focusing on the three major areas of recent investigation: the enzymology of the protein; its posttranslational regulation by reversible protein phosphorylation and opposing metabolite effectors; and the structure, expression, and molecular evolution of the nuclear PEPC genes. It is hoped that the next ten years will be equally enlightening, especially with respect to the three-dimensional …