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A Homolog Of Escherichia Coli Reca Protein In Plastids Of Higher Plants, H. Osman, P. Grandoni, Heriberto D. Cerutti, André Jagendorf
A Homolog Of Escherichia Coli Reca Protein In Plastids Of Higher Plants, H. Osman, P. Grandoni, Heriberto D. Cerutti, André Jagendorf
Center for Plant Science Innovation: Faculty and Staff Publications
Studies of chloroplast DNA variations, and several direct experimental observations, indicate the existence of recombination ability in algal and higher plant plastids. However, no studies have been done of the biochemical pathways involved. Using a part of a cyanobacterial recA gene as a probe in Southern blots, we have found homologous sequences in total DNA from Pisum sativum and Arabidopsis thaliana and in a cDNA library from Arabidopsis. A cDNA was cloned and sequenced, and its predicted amino acid sequence is 60.7% identical to that of the cyanobacterial RecA protein. This finding is consistent with our other results showing …