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Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino Aug 2014

Expression And Functional Characterization Of Mtp Genes From Arabidopsis Thaliana In Saccharomyces Cerevisiae, Yessica E. Cerino

Theses and Dissertations - UTB/UTPA

In this study the functional characterization and overexpression of Metal Tolerance Protein (MTP) cDNA were evaluated using a transgenic yeast (Saccharomyces cerevisiae) model system as a preliminary means for the characterization of metal tolerance conferred by putative Group III MTP genes from Arabidopsis thaliana. Metal disc assays for nickel, zinc, cobalt and cadmium treatments were performed to measure the tolerance imparted to yeast by the MTP cDNAs. Statistical analysis from zinc and cadmium assays showed AtMTP1 imparted the highest zinc tolerance in yeast, while AtMTPB1 imparted the highest cadmium and cobalt tolerance. However, AtMTPA2 and AtMTPB produced a decrease in …


Monitoring Citrus Nutrition In The Rio Grande Valley For Fertilizer Recommendations, Iram Lopez May 2014

Monitoring Citrus Nutrition In The Rio Grande Valley For Fertilizer Recommendations, Iram Lopez

Theses and Dissertations - UTB/UTPA

Soil fertility and plant nutrition is crucial for a better yield and consistent harvest in the Rio Grande Valley for local farmers growing citrus trees. A field of oranges and a field of grapefruits were monitored and nutrition deficiencies were noticed that played an important role during fruit set. Soil samples that were taken throughout the year were analyzed via a carbon dioxide extraction method that mimics the way plants naturally take up nutrients or extract nutrients from every soil type. Macronutrients such as the N, P, K, Na, Ca, Mg and some of the micronutrients (Zn, Fe, Mn, and …