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Evaluation Of The Escherichia Coli Threonine Deaminase Gene As A Selectable Marker For Plant Transformation, A. Ebmeier, L. Allison, Heriberto D. Cerutti, Thomas E. Clemente
Evaluation Of The Escherichia Coli Threonine Deaminase Gene As A Selectable Marker For Plant Transformation, A. Ebmeier, L. Allison, Heriberto D. Cerutti, Thomas E. Clemente
Center for Plant Science Innovation: Faculty and Staff Publications
The initial step in the synthesis of isoleucine (Ile) is the conversion of threonine to α-ketobutyrate. This reaction is carried out by threonine deaminase (TD), which is feedback-regulated by Ile. Mutations in TD that manifest insensitivity to Ile feedback inhibition result in intracellular accumulation of Ile. Previous reports have shown that in planta expression of the wild-type Escherichia coli TD, ilvA, or an Ile-insensitive mutant designated ilvA-466, increased cellular concentrations of Ile. A structural analog of Ile, L-O-methylthreonine (OMT), is able to compete effectively with Ile during translation and induce cell death. It has been postulated that OMT …