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Plant Sciences

University of Massachusetts Amherst

Theses/Dissertations

Phytosiderophores

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Grass-Specific Mechanisms Of Iron Uptake: Investigation Of Phytosiderophore Transporters And Discovery Of Novel Iron Deficiency Loci, David Chan Rodriguez Oct 2018

Grass-Specific Mechanisms Of Iron Uptake: Investigation Of Phytosiderophore Transporters And Discovery Of Novel Iron Deficiency Loci, David Chan Rodriguez

Doctoral Dissertations

Iron-deficiency anemia is one of the most prevalent forms of malnutrition worldwide, affecting 1.62 billion people, with the population in developing countries being the most affected. Iron is equally vital in plants to perform essential functions such as photosynthesis. Crop grasses form part of everyday human nutrition, contribute fundamentally to human caloric intake, and, in some parts of the world, are the primary source of food. Grasses acquire iron from the soil by secreting chelator molecules called phytosiderophores to solubilize iron, making it available to be transported by the Yellow Stripe1 (YS1) transporter. In this dissertation, I studied aspects of …


Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo Nov 2014

Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo

Masters Theses

The work presented here focuses on the molecular mechanism of phytosiderophore secretion in graminaceous plants. In maize, yellow stripe3 (ys3) is a mutant that is deficient in its ability to secrete iron-chelating compounds of the mugineic acid family known as phytosiderophores. Phytosiderophores are specific to grasses and are used for the acquisition of iron. Genetic linkage mapping of the ys3 locus lead to a region of interest on chromosome 3 defined by marker UMC1773. The sequence of eleven candidate genes (GRMZM2G390345, GRMZM2G390374, GRMZM2G342821, GRMZM5G800764, GRMZM2G502560, GRMZM5G849435, GRMZM2G105766, GRMZM5G876835, GRMZM2G036976, GRMZM2G502563, miR167g) revealed several small deletions …