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- Aegle marmelos (4)
- Ammi majus (3)
- Desert restoration (3)
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- Histology (2)
- In vitro (2)
- Land suitability (2)
- Mango (2)
- Micropropagation (2)
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- A Reliable and High Yielding Method for Isolation of Genomic DNA from Ammi majus. (1)
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- Agrobacterium tumefaciens (1)
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Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati
Progress And Future Prospects Of Developing Transgenics In Mango, Rajesh Pati
Rajesh Pati
Mango (Mangifera indica L.) is the most important fruit crop of the world. India ranks first among world’s mango producing countries accounting for 57.18 % of the total world mango production of 19.22 million tones. With the development of rDNA technology, plant breeders have access to a large number of genes that can be integrated into the plant genome.
Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati
Histological And Biochemical Changes In Aegle Marmelos, Rajesh Pati
Rajesh Pati
Aegle marmelos Corr., belongs to family Rutaceae, is more prized for its pharmacological virtues than its edible quality. Because of pharmacological importance, it’s become potential candidate for developing transgenics to enhance its medicinal properties. Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage, and field established plants).
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Somatic Embryogenesis In Mono And Poly Embryonic Cultivars, Rajesh Pati
Rajesh Pati
A standard uniform protocol of regeneration is the prime and foremost prerequisite for not only improve the productivity, the production from the existing area but also development of transgenics in mango for various traits. Polyembryonic cultivars are good rootstock because of moderate canopy, tolerant to draught, salt, environmental stress (wind, high temperature, air pollution), fungal disease (anthracnose, powdery mildew, gummosis) and hoppers.
Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati
Genetic Transformation Of Bael (Aegle Marmelos Corr.), Rajesh Pati
Rajesh Pati
Aegle marmelos Corr. is better known for its medicinal values rather than its edible quality. Every part of the plant viz. leaves, fruits, roots, and the bark are used for treating various diseases related to heart, stomach, bacterial, fungal, viral, worms, fertility, fever and cosmetics. Marmelosin is one of the major compounds (coumarin), used to treat various diseases. Till date, there are no varieties developed for commercial orchards of Bael and hence, regarded as one among the underutilized fruits of India. Therefore, there is an urgent need for breeding new and cultivable varieties in Aegle marmelos. But the problem for …
Micropropagation In Fruit Crops, Rajesh Pati
Micropropagation In Fruit Crops, Rajesh Pati
Rajesh Pati
Micropropagation is a proven means of producing millions of identical plants under a controlled and aseptic condition, independent of seasonal constraints. It not only provides economy of time and space but also gives greater output and allows further augmentation of elite disease free propagules. India is homeland of many important fruit crops such as Indian gooseberry (Emblica officinalis Gaertn), bael (Aegle marmelos Corr.), Guava (, Psidium guajava), jamun or black plum (Syzygium cuminii L. Skeels.), Mango (Mangifera indica) and Papaya (Carica papaya).
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
In Vitro Plant Regeneration And Genetic Assessment Among Regenerates Using Molecular Markers In Aegle Marmelos Corr., Rajesh Pati
Rajesh Pati
Bael (Aegle marmelos Corr.) is an important medicinal fruit tree. The fruit pulp contains marmelosin, which is a laxative, diuretic, is being used in many patented drugs in India. Micropropagation technology can be gainfully employed in mass multiplication of improved bael varieties. We have developed micropropagation protocol of bael through shoot bud culture. It was imperative to test genetic fidelity of micropropagated plants using molecular markers (RAPD, DAMD and ISSR).
Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati
Histological And Biochemical Changes In Aegle Marmelos Corr. Before And After Acclimatization, Rajesh Pati
Rajesh Pati
Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (In vitro stage, acclimation stage). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO2/h/g fresh weight) and but higher protein (0.048 μg/g) during in vitro phase. The in vitro raised plants showed abnormal histological features like altered leaf mesophyll, absence …
Semi-Automated Classification Of Gray Scale Aerial Photographs Using Geographic Object Based Image Analysis (Geobia) Technique, Ahmed Harb Rabia, Fabio Terribile
Semi-Automated Classification Of Gray Scale Aerial Photographs Using Geographic Object Based Image Analysis (Geobia) Technique, Ahmed Harb Rabia, Fabio Terribile
Ahmed Harb Rabia A.H. Rabia
Aerial photography is an important source of high resolution remotely sensed data. Before 1970, aerial photographs were the only remote sensing data source for land use and land cover classification. Using these old aerial photographs improve the final output of land use and land cover change detection. However, classic techniques of aerial photographs classification like manual interpretation or screen digitization require great experience, long processing time and vast effort. A new technique needs to be developed in order to reduce processing time and effort and to give better results. Geographic object based image analysis (GEOBIA) is a newly developed area …
Land Suitability Analysis For Policy Making Assistance: A Gis Based Land Suitability Comparison Between Surface And Drip Irrigation Systems, Ahmed Harb Rabia A.H. Rabia, H. Figueredo, T. L. Huong, B. A. A. Lopez,, H. W. Solomon, V. Alessandro
Land Suitability Analysis For Policy Making Assistance: A Gis Based Land Suitability Comparison Between Surface And Drip Irrigation Systems, Ahmed Harb Rabia A.H. Rabia, H. Figueredo, T. L. Huong, B. A. A. Lopez,, H. W. Solomon, V. Alessandro
Ahmed Harb Rabia A.H. Rabia
Land suitability assessment for irrigation is a very important tool not only in terms of agriculture development planning, but also to overcome the global problem of water scarcity. The aim of this study is to spatially evaluate land suitability of the study area, Kilte Awulaelo district in Ethiopia, for surface and drip irrigation methods based on GIS and remote sensing approaches. This work has been done as a part of the 29th Course Professional Master in Istituto Agronomico per l’Oltremare, Florence, Italy. Final suitability maps show the irregularity of suitability classes’ distribution over the study area. Results show that only …
Introducing A New Parametric Concept For Land Suitability Assessment, Ahmed Harb Rabia
Introducing A New Parametric Concept For Land Suitability Assessment, Ahmed Harb Rabia
Ahmed Harb Rabia A.H. Rabia
In an ecosystem, there is need to establish the quantity and quality of resources and their suitability for a certain range of land uses in order to assure its future productivity and sustainability of biodiversity. Parametric methods are widely used for land suitability evaluation. A new parametric concept “equation” of land suitability evaluation has been proposed to improve results of land suitability evaluation. Land suitability assessment for wheat production was conducted in order to compare results of the suggest method with classical parametric methods. Organic matter, CaCO3, pH, Slope, texture, drainage, depth, EC and altitude were recognized as factors affecting …
Travelling In Cuba, Fathi Habashi
Travelling In Cuba, Fathi Habashi
Fathi Habashi
Contact with Cuban scientists was established in 1986 when two researchers from Centro de Investigaciones para Industria Minero Metalurgica in Havana visited Laval University to get acquainted with research activities in the area of extractive metallurgy. This was followed in early 1987 by two more researchers from the same centre. Invitations were then received in late 1987, 1989, and 2008 to visit the nickel refineries in Cuba and to hold seminars at the University of Havana. The present report outlines these activities.
Deep Pipe Irrigation, David A. Bainbridge
Deep Pipe Irrigation, David A. Bainbridge
David A Bainbridge
Deep pipe irrigation uses a vertically placed section of pipe to deliver water to the roots of plants. This reduces evaporation and increases water use efficiency. Deep pipes can be filled by hand, drip or rainwater catchment.
Planting Desert Sites, David A. Bainbridge
Planting Desert Sites, David A. Bainbridge
David A Bainbridge
Desert planting requires great care in preparing plants, handling plants and planting. With proper consideration of soil, climate and water availability plants can be established despite severe conditions of high temperatures, high winds, herbivory and lack of water.
Container Plants For Desert Restoration, David A. Bainbridge
Container Plants For Desert Restoration, David A. Bainbridge
David A Bainbridge
Container planting is essential for most desert restoration work because direct seeding is rarely successful. The containers best suited for desert planting are often taller (deeper) to facilitate root development. Fertilizer management, inoculation, irrigation and pruning can be used to maximize root growth with minimal shoot development.
Organogenesis Of Ammi Majus Via Callus Culture From Leaf., Sandip S. Magdum, Sanjeev Kumar
Organogenesis Of Ammi Majus Via Callus Culture From Leaf., Sandip S. Magdum, Sanjeev Kumar
Sandip S. Magdum
Ammi majus L. is an imperiled medicinal plant, which contains various medically important and uncommon extraordinary secondary metabolite compounds. An efficient organogenesis protocol was established for Ammi majus through in vitro process of leaf callusing, shooting and rooting by using different combination of auxins and cytokinins. In vitro cultured juvenile leaf explants were used directly for callus formation on MS supplemented with different concentration of IAA, Kn and CH. Maximum percentage response for callus formation was 97.7 after 5 weeks of incubation on MS supplemented with 2 mg/l IAA, 2 mg/l Kn and 1000 mg/l CH. About 81% of calluses …
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
Sandip S. Magdum
The developed protocol describes a cheaper, quicker and reliable method for the isolation of pure DNA from medicinal herbs, such as Ammi majus, which produces the secondary metabolites xanthotoxin and berganpectane having immense medicinal importance. Use of CTAB, liquid nitrogen and EDTA in different isolation protocols analyzed for A. majus, all were ended with polysaccharide and protein contamination with low purity of DNA (A260/280 = 1.3 – 1.6), revealed a need for method modification for the inexpensive and rapid isolation of pure DNA. Developed reliable and competent protocol isolated enough pure DNA (A260/280 = 1.81) without following time consuming lengthy …
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Sandip S. Magdum
Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 …
Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.
Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.
Halijah Ibrahim
BACKGROUND: Alpinia pahangensis, a wild ginger distributed in the lowlands of Pahang, Malaysia, is used by the locals to treat flatulence. In this study, the antioxidant and cytotoxic activities of the crude aqueous methanol and fractionated extracts of Alpinia pahangensis against five different cancer and one normal cell lines were investigated. The total phenolic content of each extract and its fractions were also quantified. This is the first report on the antioxidant and cytotoxic activities of Alpinia pahangensis extract. METHODS: In the current study, the crude methanol and fractionated extract of the rhizomes of Alpinia pahangensis were investigated for their …