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Plant Sciences

Brigham Young University

BioNano physical mapping

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Assembly, Annotation And Optical Mapping Of The A Subgenome Of Avena, Rebekah Ann Lee Dec 2017

Assembly, Annotation And Optical Mapping Of The A Subgenome Of Avena, Rebekah Ann Lee

Theses and Dissertations

Common oat (Avena) has held a significant place within the global crop community for centuries; although its cultivation has decreased over the past century, its nutritional benefits have recently garnered increased interest for human consumption. No published reference sequences are available for any of the three oat subgenomes. Here we report a quality sequence assembly, annotation and hybrid optical map of the A-genome diploid Avena atlantica Baum and Fedak. The assembly is composed of a total of 3,417 contigs with an N50 of 11.86 Mb and an estimated completeness of 97.6%. This genome sequence will be a valuable research tool …


Assembly, Annotation And Optical Mapping Of The A Subgenome Of Avena, Rebekah Ann Lee Dec 2017

Assembly, Annotation And Optical Mapping Of The A Subgenome Of Avena, Rebekah Ann Lee

Theses and Dissertations

Common oat (Avena) has held a significant place within the global crop community for centuries; although its cultivation has decreased over the past century, its nutritional benefits have recently garnered increased interest for human consumption. No published reference sequences are available for any of the three oat subgenomes. Here we report a quality sequence assembly, annotation and hybrid optical map of the A-genome diploid Avena atlantica Baum and Fedak. The assembly is composed of a total of 3,417 contigs with an N50 of 11.86 Mb and an estimated completeness of 97.6%. This genome sequence will be a valuable research tool …


Improving Cotton Agronomics With Diverse Genomic Technologies, Aaron Robert Sharp Mar 2016

Improving Cotton Agronomics With Diverse Genomic Technologies, Aaron Robert Sharp

Theses and Dissertations

Agronomic outcomes are the product of a plant's genotype and its environment. Genomic technologies allow farmers and researchers new avenues to explore the genetic component of agriculture. These technologies can also enhance understanding of environmental effects. With a growing world population, a wide variety of tools will be necessary to increase the agronomic productivity. Here I use massively parallel, deep sequencing of RNA (RNA-Seq) to measure changes in cotton gene expression levels in response to a change in the plant's surroundings caused by conservation tillage. Conservation tillage is an environmentally friendly, agricultural practice characterized by little or no inversion of …