Digital Commons Network^™

Assessment Of Genetic Diversity In The Usda And Cip-Fao International Nursery Collections Of Quinoa (Chenopodium Quinoa Willd.) Using Microsatellite Markers, S. A. Christensen, D. B. Pratt, C. Pratt, P. T. Nelson, M. R. Stevens, Eric N. Jellen, Craig E. Coleman, Daniel J. Fairbanks, A. Bonifacio, Michael J. Maughan

Faculty Publications

Quinoa (Chenopodium quinoa Willd.) is a staple food crop for millions of impoverished rural inhabitants of Andean South America where it has been cultivated for millennia. Interest in quinoa, due largely to its superior nutritional characteristics, is fuelling a growing export market and has led to an increased focus on genetic research and the development of quinoa breeding programmes throughout South America. The success of these breeding programmes will rely heavily on the development of core germplasm collections and germplasm conservation. We report the development of a set of fluorescence-tagged microsatellite molecular markers that can be used to characterize genetic …

Spotted Cotton Oligonucleotide Microarrays For Gene Expression Analysis, Joshua Udall, Lex E. Flagel, Foo Chung, Andrew W. Woodward, Ran Hovav, Ryan A. Rapp, Jordan M. Swanson, Jinsuk J. Lee, Alan R. Gingle, Dan Nettleton, Christopher D. Town, Z. Jeffrey Chen, Jonathan F. Wendel

Faculty Publications

Microarrays offer a powerful tool for diverse applications plant biology and crop improvement. Recently, two comprehensive assemblies of cotton ESTs were constructed based on three Gossypium species. Using these assemblies as templates, we describe the design and creation and of a publicly available oligonucleotide array for cotton, useful for all four of the cultivated species. Results: Synthetic oligonucleotide probes were generated from exemplar sequences of a global assembly of 211,397 cotton ESTs derived from <50 different cDNA libraries representing many different tissue types and tissue treatments. A total of 22,787 oligonucleotide probes are included on the arrays, optimized to target the diversity of the transcriptome and previously studied cotton genes, transcription factors, and genes with homology to Arabidopsis. A small portion of the oligonucleotides target unidentified protein coding sequences, thereby providing an element of gene discovery. Because many oligonucleotides were based on ESTs from fiber-specific cDNA libraries, the microarray has direct application for analysis of the fiber transcriptome. To illustrate the utility of the microarray, we hybridized labeled bud and leaf cDNAs from G. hirsutum and demonstrate technical consistency of results. Conclusion: The cotton oligonucleotide microarray provides a reproducible platform for transcription profiling in cotton, and is made publicly available through http://cottonevolution.info.

Water In Beaver Dam Wash And Lytle Preserve, Stanley L. Welsh

Books by Faculty of the Monte L. Bean Life Science Museum

This short work is derived from a manuscript prepared for presentation in a water adjudication hearing, following a proposal to pipe water from wells in Beaver Dam Wash for transfer across state lines to be used for culinary and other purposes in Mesquite, Nevada. The Wash is estimated to yield 2000 acre feet of water annually, mostly as subsurface flow, at the Arizona line. About a third of that amount is already appropriated by users along the Wash in Utah, but there are applications pending for more than 100,000 acre feet in Utah. Thus, the Wash is already oversubscribed by …

Genetic Diversity And Low Reproductive Success In Isolated Populations Of Utah Juniper (Juniperus Osteosperma, Cupressaceae), Loreen Allphin, Val J. Anderson, Amy F. Hunt

Faculty Publications

Utah juniper (Juniperus osteosperma) has greatly expanded its historical range in the western United States. Management plans for the species have focused on curtailing its encroachment into sagebrush and grassland communities. These plans often include burning or other methods of elimination. These methods may result in subdivision or fragmentation of existing juniper stands. We initiated a study at Dugway Proving Ground, a U.S. Army facility, to examine the effects of fragmentation on the reproductive success of Utah juniper in isolated populations. We used enzyme electrophoresis to quantify genetic variability in isolated populations. We also determined population reproductive success by examining …

North American Species Of Astragalus Linnaeus (Leguminosae): A Taxonomic Revision, Stanley L. Welsh

Books by Faculty of the Monte L. Bean Life Science Museum

Abstract

This summary revision of the species of Astragalus in North America, north of Mexico, was initially prepared for inclusion in the Flora North America Project. That publication has been postponed indefinitely, and this paper is being presented now to allow those students of the genus with an updated manuscript to review, and to use prior to appearance of the Flora North America treatment. It differs from the Flora North America format in having bibliographic citations to the synonyms, and the type specimens are cited. The present work treats 354 species; 198 varieties; 552 total taxa. Should the 4 7 …

Response Of Three Ecotypes Of Maca (Lepidium Meyenii Walp.) To Five Levels Of Ovine Manure In The Community Of Yampupata (Manco Kapac Province - La Paz), Sabina Quispe Paye

Theses and Dissertations

This study was conducted in the community of Yampupata, in the municipality of Copacabana, in the Manco Kapac province, La Paz, to an altitude of 3841 m.a.s.l, agricultural campaign 2003-2004. The purpose of the study was to determine and compare the fenological stages (germination, growth, leafing, and harvesting), hypocotyl yield (dry and fresh), classification of the hypocotyl (diameter, length, etc.), and the economical evaluation as well. The experiment set up was installed in the lower region of the community, dedicated to agriculture, performing all the aforementioned activities together for the period of a year. The experimental design was of Random …

Adaptations Of Five Pale-Type Grain Amaranth Accessions (Amaranthus Caudatus) And Five Ataco Or Sangorache Accessions (Amaranthus Hybridus) In The Cities Of Otavalo And Antonio Ante, Sixto Stalin Tuston Torres

Theses and Dissertations

This study was conducted in two locations: Pucará (2,413m above sea level) and Mojandita (2,922m above sea level), both of which are found in the province of Imbabura, Ecuador. The objectives of this study were to: characterize the morphological and agronomic traits as well as the overall quality of the ten amaranth accessions in this study; identify the accessions with the highest yield and biomass; determine the nutritional characteristics of the experimental accessions; and determine the cost of production of amaranth per hectare. The following null hypothesis was assumed: the agronomic and nutritional characteristics of the ten accessions in this …