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Identification Of Carrying Alien Dna Fragments In Solanum Melongena X Solanum Incanum Interspecific Progeny By Using Cosii Marker, Görkem Sülü, İlknur Polat, Hati̇ce Fi̇li̇z Boyaci, Ahmet Naci̇ Onus

Turkish Journal of Botany

Cultivated eggplant (Solanum melongena L.) is produced in many countries with temperate and tropical climates and has great economic importance. In recent years, resistance to biotic and abiotic stress conditions in addition to increasing yield and quality has gained importance in plant breeding including eggplant breeding. Therefore, the wild relative S. incanum is an important parent in breeding studies as it provides resistance to some important biotic and abiotic stresses for eggplant. It is possible to obtain a fully fertile hybrid between the two species, as well as to establish F2 lines. However, it is a mystery whether there is …

Potential Hybridization Among Two Species Of California Salvia, Ellie Morrison, Bryan Drew

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Hybridization among plants is fairly common, particularly in instances where closely related species are sympatric. One example of a group in which hybridization has been detected is the genus Salvia (Lamiaceae; mint family). Salvia is a diverse genus consisting of about 1,000 species and is defined primarily by having only two stamens, each with their anther sacs separated by elongated connective tissue. The genus has diversity centers around the world, including Southwest Asia and the Mediterranean region, Mexico/Central America, northern and central South America, and temperate East Asia. The genus also has a smaller species radiation of 19 species in …

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Research in Biology

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott

Faculty Works

The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …

Plants, Pipettes And Pcr, Elizabeth Vierling

Science and Engineering Saturday Seminars

Plants are amazing organisms that provide us with food, building materials, the pleasure of gardens, as well as providing the foundation of critical world ecosystems. Although they may look like they are just stuck in one place and doing not more than growing, they have many, many complex ways in which they respond to the environment. The goal of this STEM Ed session will be to discuss ways that plants can respond to the environment with hands on exercises and exploration of possible classroom activities. Participants will engage in state-of-the art methods of testing plant DNA composition using the polymerase …

Prevalence Of Dhp Toxicity And Detection Of S. Jonesii In Ruminants Consuming Leucaena Leucocephala In Eastern Indonesia, Michael J. Halliday, Tanda Panjaitan, Jacob Nulik, H. Dahlanuddin, Jagadish Padmanabha, Chris S. Mcsweeney, Sulaiman Depamede, Debora Kana Hau, Kurniawan, Muhammad Fauzan, Sutartha, Baiq Tutik Yuliana, Charles Pakereng, Putri Ara, Dessy Liubana, Resti G. Edison, H. Max Shelton

IGC Proceedings (1997-2023)

Leucaena leucocephala (leucaena) is a productive forage tree legume widely used in eastern Indonesia. While highly nutritious, it possesses the toxin mimosine which adversely affects animal production. In ruminants, mimosine is readily converted to the two isomers of dihydroxypyridine (3,4-DHP and 2,3-DHP) known to cause goitre, suppress appetite, and cause severe mineral deficiencies. These adverse symptoms may be partially responsible for the reluctance of some farmers to feed leucaena.

A bacterium capable of complete degradation of DHP, Synergistes jonesii, originally discovered in Hawaii in goats consuming leucaena (Jones and Megarrity 1986), was later found in Indonesia which led to …

Molecular Gut-Content Analysis Reveals High Frequency Of Helicoverpa Zea (Lepidoptera: Noctuidae) Consumption By Orius Insidiosus (Hemiptera: Anthocoridae) In Sweet Corn, Julie A. Peterson, E. C. Burkness, James D. Harwood, W. D. Hutchison

Department of Entomology: Faculty Publications

Management of corn earworm Helicoverpa zea in sweet corn grown for processing can be challenging due to the lack of effective transgenic and chemical control options. However, biological control by generalist predators can provide a significant impact on pests in this cropping system. One of the most ubiquitous predators of H. zea and other lepidopterans is the insidious flower bug, Orius insidiosus. This small hemipteran has been observed as an important mortality agent of H. zea in several cropping systems, but the strength of the trophic connection between these species has not been documented in sweet corn. Molecular gut-content …

Selection Of Efficient Taq Dna Polymerase To Optimize T-Dna Genotyping Method For Rapid Detection Of Mutant Arabidopsis Thaliana Plants, Inna B. Chastukhina, Liliia R. Nigmatullina, Lia R. Valeeva, Eugene V. Shakirov

Biological Sciences Faculty Research

Plants harbor homologues of various animal genes involved in phosphorus metabolism, telomere biology and other cellular processes. Compared to experiments with many other multicellular organisms, research in the model plant Arabidopsis thaliana takes advantage of short generation time and an ever increasing arsenal of genetic and transgenic tools, including large collections of T-DNA knockout and activation lines. The availability of thousands of publicly available transgenic Arabidopsis lines provides a unique opportunity to address a number of important biological questions. However, identification of individual T-DNA mutant plants from a pool of seeds provided by a biological stock distribution center remains a …

Lof1 And Interacting Transcription Factors In Plant Development And Crop Yield, Zoe Yeoh, Elizabeth Luscher, Patricia Springer

Student Publications

Transcription factors (TFs) help ensure proper gene expression in developing tissues, and thus play a role in plant development and plant architecture. LATERAL ORGAN FUSION1, or LOF1, is a TF expressed in the organ boundaries of Arabidopsis thaliana. lof1 mutants have fused axillary branches and cauline leaves, which indicates importance in boundary development. Because transcription factors are known to act in complexes, we wanted to discover what other proteins interact with LOF1. We executed a yeast-2-hybrid (Y2H) screen that identified several TFs as potential interactors: WHIRLY 3 (WHY3), MYB DOMAIN PROTEIN32 (MYB32), HOMEOBOX-LEUCINE ZIPPER PROTEIN4 (HB4), and LIGHT RESPONSE BTB2 …

Phages Of Non-Diary Lactococci: Isolation And Characterization Of Phi L47, A Phage Infecting The Grass Isolate Lactococcus Lactis Ssp Cremoris Dpc6860, Daniel Cavanagh, Caitríona M. Guinane, Horst Neve, Aidan Coffey, R. Paul Ross, Gerald F. Fitzgerald, Olivia Mcauliffe

Department of Biological Sciences Publications

Lactococci isolated from non-dairy sources have been found to possess enhanced metabolic activity when compared to dairy strains. These capabilities may be harnessed through the use of these strains as starter or adjunct cultures to produce more diverse flavor profiles in cheese and other dairy products. To understand the interactions between these organisms and the phages that infect them, a number of phages were isolated against lactococcal strains of non-dairy origin. One such phage, ΦL47, was isolated from a sewage sample using the grass isolate L. lactis ssp. cremoris DPC6860 as a host. Visualization of phage virions by transmission electron …

Chlorolepiota Brunneotincta: A New Species (Agaricaceae) From India, Narender Singh Atri, Babita Kumari, Ramesh Chandra Upadhyay

Turkish Journal of Botany

The genus Chlorolepiota Sathe & Deshpande is a monotypic genus represented by C. mahabaleshwerences Sathe & Deshpande. In this paper, a new species, Chlorolepiota brunneotincta, is described, based upon macroscopic, microscopic, and molecular studies undertaken on the collection from Solan Himachal Pradesh (India).

Differential Expression Of P1f1-Targeted Genes In Various Pif1 And Ctg10 Mutants, Taylor D. Lloyd

Kaleidoscope

No abstract provided.

Morphological, Chemical, And Molecular Analyses Of Turkish Papaver Accessions (Sect. Oxytona), İskender Parmaksiz, Sebahatti̇n Özcan

Turkish Journal of Botany

The species of the section Oxytona Bernh., classified under the genus Papaver L., are of great importance due to their medical alkaloid contents such as morphine, codeine, and thebaine. A clearly established classification system is not available for Oxytona species. The aim of this study was to find relationships among 53 accessions belonging to 3 important Oxytona species, i.e. Papaver bracteatum Lindl., P. orientale L., and P. pseudo-orientale (Fedde) Medw., using 19 different morphological characters, 1 chemical character, and 15 random amplified polymorphic DNA (RAPD) markers. Nineteen characters, including bract and sepal leaves, petal marks, and capsule shape and size …

Efficacy Of Different Detergents For Leaf Extract Based Sex Diagnostics Of Papaya (Carica Papaya L.)., Rajesh Pati

Rajesh Pati

No abstract provided.

In Vitro Clonal Propagation Of Bael (Aegle Marmelos Corr.) Cv. Cishb1 Through Enhanced Axillary Branching, Rajesh Pati

Rajesh Pati

Rapid clonal micropropagation protocol of Aegle marmelos (L.) Corr. cv. CISH-B1 was achieved by nodal stem segment of mature bearing tree. Three centimeter long shoots having one axillary bud excised from 10-15th nodal region of shoots during September gave quick in vitro bud burst (5.33 days) when cultured on MS medium supplemented with BAP, 8.84 μM + IAA 5.7 μM. The maximum number of proliferated shoots (9.0/explant) were obtained on same medium supplemented with BAP 8.84 μM + IAA 5.7 μM. The micro shoots were rooted (100 %) on ½ strength MS medium supplemented with IBA 49.0 + IAA 5.7 …

Comparative Structural Study Of Leaf Spot Disease Of Safflower And Sugar Beet By Cercospora Beticola, Robert T. Lartey, Andrew W. Lenssen, Robert G. Evans, Soumitra Ghoshroy

Andrew W. Lenssen

Sugar beet and safflower are sometimes rotated or grown side by side in the Sidney, MT region of the Lower Yellowstone River Basin (LYRB). Cercospora beticola and C. carthami infect sugar beet (Beta vulgaris) and safflower (Carthamus tinctorius) respectively. C. beticola is ubiquitous in sugar beet, but C. carthami has not been reported in LYRB. Observations of unusual leaf spots on safflower in Sidney led to investigation and subsequent identification of safflower as a host of C. beticola. We describe a comparative structural study of progression of C. beticola infection and disease development in both sugar beet and safflower. The …