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A Beginner's Guide To Growing Mushrooms At Home, Jacob Hawkes, Paul R. Grossl, Melanie Stock

All Current Publications

Welcome to the wonderful world of mushrooms! Researchers have shown that mushrooms are not only nutritious and delicious but also provide a myriad of known medicinal benefits, such as protection against cancer, protecting brain health, stimulating gut health, and supporting a healthy immune system. Lately, psychedelic mushrooms have been receiving considerable attention as a promising treatment for mental health conditions, such as depression, post-traumatic stress disorder and end-of-life anxiety among the terminally ill. This publication provides a simple guide for beginners interested in growing basic gourmet and medicinal mushrooms at home for personal use using easily accessible, relatively inexpensive materials.

Potential Biological Control Agents For Nassella Neesiana (Poaceae) Invading Australian Native Grasslands, F. E. Anderson, M. L. Díaz, D. A. Mclaren

IGC Proceedings (1997-2023)

The introduction and proliferation of exotic stipoid grasses over the past 100 years seriously threatens agricultural productivity and the integrity of Australia's indigenous flora and fauna, particularly its grasslands (McLaren et al., 1998). The full effect on biodiversity by the spread of these grasses is unknown but likely to be major (Hocking, 1998). Conventional control techniques have not stopped the invasion adequately, so it is a priority to find control options to achieve an effective management strategy. A biological control project against Nassella trichotoma and N. neesiana was initiated in 1999 in Argentina. We report on the most recent …

Considerations For The Use Of Mycorrhizal Fungi Inoculants In Coal Mine Reclamation And Reforestation In Appalachia: A Guide Of Best Practices And Management Recommendations, Cole Hansen Reistrup

Master's Projects and Capstones

Nearly all terrestrial plants form symbiotic relationships with mycorrhizal fungi. These beneficial soil microbes support plant health by increasing plants’ access to nutrients and water, protecting them from pathogens, improving soil properties, and more. A challenge to restoring forests on coal mined land in Appalachia is that mine soils are often degraded in a number of ways, including lacking mycorrhizal fungi, which makes mine soils difficult for trees to grow upon. Substantial improvements to coal mine reforestation success have been made since the development of the Forestry Reclamation Approach in 2005 that provides guidelines for how to create a suitable …

Comparing Fungal Communities In Tsuga Heterophylla Seedling Roots Across Nurse Log Decay Stages And The Forest Floor, Ellie Olpin, Reisha Foertsch

Summer Research

Nurse logs are fallen, decaying trees that have been shown to facilitate the survival, establishment, and growth of tree seedlings, therefore making vital contributions to forest regeneration. Plant-fungi interactions may play a role in influencing seedling survival across nurse log decay and the forest floor. We sought to examine how fungal communities in seedling roots change between nurse logs and forest floor as well as across nurse log decay stages. To study this, we collected western hemlock (Tsuga heterophylla) seedlings (5-10 cm) from nurse logs of each decay stage and the forest floor at three field sites on the Olympic …

The Effects Of Ericoid Inoculum Source And Nutrient Addition On Growth And Reproduction In Highbush Blueberry, Ilana Williams

Graduate College Dissertations and Theses

Ericoid mycorrhizal fungi (EMF) form symbiotic relationships with ericaceous plants such as Vaccinium corymbosum, or highbush blueberry and assist in nutrient acquisition. EMF help plants thrive in stressful environments by increasing the area in which roots can uptake water and nutrients. In plant-mycorrhizal symbioses, nutrient uptake may depend on the identity of the fungal partner. Therefore, differently sourced mycorrhizal fungi could show differences in nutrient uptake ability. Here, I hypothesized that inoculation of V. corymbosum with EMF would enhance plant growth and investment in reproduction, and that effect would be more pronounced for plants in low nutrient conditions. I also …

Potentials Of Pleurotus: Reimagining The Relationship Between Cattle And Brewer's Spent Grain, Zoe Stojkovic

Senior Projects Spring 2023

Brewer’s spent grains (BSGs), a byproduct from beer production, are generated in excess globally. Most often, they are sold or given to proximate cattle farmers for use as feed. However, spent grain can also be used as a medium for fungal cultivation. Given that certain fungal species have the capability to degrade lignin and produce protein, the cultivation of fungi on spent grains may serve to enhance the nutritional profile of the grains for their use as cattle feed. This project is an effort to determine the compatibility of fungal cultivation with BSGs in order to both improve upon cattle …

The Addition And Cessation Of Inorganic Fertiliser Amendments In Long-Term Managed Grasslands: Impacts On Above And Below-Ground Communities, C. D. Clegg, P. J. Murray, R. Cook, T. Tallec

IGC Proceedings (1997-2023)

In recent times, land use in the United Kingdom has undergone considerable changes because of social and economic pressures, leading to a fine balance between the demands of highly productive intensive systems and practices which are perceived to be more environmentally acceptable. Plant productivity is governed by the supply of nutrients from the soil, which in turn is dependent on the dynamics of organic matter decomposition driven by soil micro-, meso- and macro fauna. Considerable information is available concerning the impact of inorganic fertiliser additions on communities of macro-fauna and flora, but the effects on specific microbial communities in soils …

The Influence Of Burning On Soil Microbial Biomass And Activity Along The Boro Route In The Okavango Delta Of Botswana, Tabo Mubyana-John, A. Banda

IGC Proceedings (1997-2023)

The Okavango Delta, a protected area in northeastern Botswana because of its annual flooding pattern, is the main source of water in an otherwise arid environment with a high diversity of plants and animals and forms the main tourist area in the region. However, the area is under threat from range fires. The objective of this study was to assess the influence of fire on soil microbial activity, biomass C, fungal population and diversity, and some soil properties along the Boro route of the Okavango Delta (Botswana).

Identification Of Cytospora Species Isolated From Canker In Peach Trees In South Carolina, Stephen T. Baker

All Theses

In 2016, a survey was conducted into the causes of twig blight on peach (Prunus persica) in five orchards across South Carolina. From this survey, isolates from the fungal genus of Cytospora (Sordariomycetes, Diaporthales) were reported on peach, for the first time, within the state. Cytospora species are known to cause oozing cankers on a wide range of woody hosts leading to dieback, loss of limbs, and reduction in yield, prompting concern for important economic crops. Past methods of identification of Cytospora species have been confounded by indistinguishable morphology, overlapping host preference among species, and genetic sequencing …

Nitrogen Availability In Dune Systems And Its Effect On Root Fungal Endophyte Communities., Haley E. Sage

College of Arts & Sciences Senior Honors Theses

As global change persists, changes in resource availability can influence plant-microbe interactions. To understand how resource availability can influence these interactions and species diversity, I focused this research on how varying nitrogen (N) levels affect root endophyte communities in the plant species Ammophila breviligulata, an ecosystem engineer in the dune system. I analyzed the relationship between nitrogen addition and microbial community composition across 60 plots treated with three nitrogen addition levels (control, low, and high) in a long-term experimental field site in the Lake Michigan dunes. I identified Ammophila breviligulata's root endophyte community after creating a culture collection and …

Functional Characterization Of A Putative Alternative Oxidase In Sporisorium Reilianum F. Sp. Zeae., Emma A Lamb

College of Arts & Sciences Senior Honors Theses

Sporisorium reilianum is a pathogenic basidiomycete fungus with two formae speciales, each capable of infecting corn (SRZ) or sorghum (SRS), respectively. This fungus is also a dimorphic variety, meaning it can switch between its haploid, yeast-like sporidia and diploid teliospore stages over the course of its life cycle (Schirawski). When S. reilianum is found in a haploid state and conditions are favorable, it will mate with a compatible non-self mating type to begin filamentous growth and proliferation in the plant host (Zhao). S. relianum, like most fungi, utilizes the four classical components of the electron transport chain to produce …

All Living Organisms Are Sentient, Arthur S. Reber, Frantisek Baluska, William B. Miller Jr.

Animal Sentience

We argue that all living organisms, from the simplest unicellular prokaryotes to Homo sapiens, have valenced experiences—feelings as states of preference—and are capable of cognitive representations. Bacteria can learn, form stable memories, and communicate, hence solve problems. Rowan et al.'s statement that "Subjective feelings are just that — subjective — and are available only to the animal (or human) experiencing them" is true but irrelevant. When we see a fish flopping about in the bottom of a boat we immediately recognize suffering without having a glimpse of the nature of piscine distress. Some controlled anthropomorphism can go a …

Soil Biodiversity, Root Herbivory And Carbon And Nitrogen Cycling In Grassland Soils, R. Cook, L. A. Dawson, A. C. Gange, S. J. Grayston, P. J. Murray, A. M. Treonis

IGC Proceedings (1997-2023)

This paper describes research on the relationships between grassland management practices and the diversity of biological communities in soil. Observations are being made in field trials with applications of nitrogen and lime and of insecticide to an original diverse sward and to a single species grass re-seed. The treatments are designed to produce different degrees of diversity in communities of soil animals and microbes. Assessments are being made over three years of the effects on the populations, activity and diversity of root-feeding animals, arbuscular mycorrhizal fungi, soil bacteria, fungi and micro fauna, including nonplant feeding nematodes. Associated laboratory experiments assess …

Possibilities To Avoid Growth Of Clostridia And/Or Fungi In Wilted Silage By Use Of Organic And Inorganic Salts, Martin Knický, P. Lingvall

IGC Proceedings (1997-2023)

The hygienic quality of silage will be of great importance in the future as poor quality not only influences the animal production but also the animal health and the food quality. This study examined the impact of mixtures of sodium benzoate (NaB), sodium nitrite (NaN), hexamine (HMTA), sodium propionate (NaP), sodium bisulphite, and propionic acid on low and high wilted clover/grass. The silage (crop wilted to 300 or 600 g DM kg^-1 of fresh weight) consisted of about 50% red clover (Trifolium pratense) and 50% timothy (Phleum pratense) and the study covered 7 additive treatments. …

Isolation Of Foliar Fungi From Roseau Cane (Phragmites Australis) In Coastal Louisiana, David M. Galo Espinal

LSU Master's Theses

Roseau cane (Phragmites australis) is a wild plant with an extensive root system that is useful in areas vulnerable to soil erosion. In Louisiana, this plant plays an important role protecting the coastal infrastructure of the lower Mississippi River delta (MRD). Currently, P. australis growing in the MRD is being affected by a die-off, which has been associated with several biotic and abiotic factors.

Plant pathogenic fungi is a biotic factor that negatively affect plant growth. Therefore, foliar fungi were isolated from plants growing in one die-off affected area (MRD) and two non-affected areas (Pecan Island and Manchac) …

Influence Of Decay Fungi On Selected Anatomical Properties Of Aningeria Robusta A. Chev. Wood, Olayiwola Olaleye Ajala, Funke Grace Adebawo, Emmanuel Adekanye Adelusi, Olaoluwa Adeniyi Adegoke

Journal of Bioresource Management

Due to population explosion, the forest of Nigeria like most tropical forest is over exploited for choice traditional species. Therefore, need to investigate species that can substitute the species arise. Five trees of Aningeria robusta were harvested from Cocoa Research Institute of Nigeria. Bolts of 50cm length were obtained at the base, middle and the top; radial strips also obtained for innerwood, middlewood and outerwood. Ten test specimens of dimension 12mm x 15mm x 25mm were obtained per zone. N-hexane heartwood extracts of a 30 year old Gliricidia sepium diluted with kerosene using volume by volume method into 0%, 25%, …

Effects Of Am Fungi From Conventional And No-Till Michigan Crop Fields On Plant And Soil Health, Derek Bennett

Masters Theses

Centuries of conventional till (CT) management in agriculture has depleted soil organic matter (SOM) by over 50%. While only comprising 5% in most soils, SOM provides soil with fertility and productivity. To compensate for SOM depletion, producers have been forced to increase their reliance on fertilizer and irrigation to maintain yields. In the coming decades, climate change is expected to challenge food production and threaten an already fragile system. With no remaining land left to cultivate, conservation management strategies such as no-till (NT) look to restore SOM and increase the resilience of food production for an ever growing, increasingly food …

Spore Dispersal Of Slime Molds And Higher Fungi Via Animal Vectors, Courtney Trimble

Graduate Theses and Dissertations

Myxomycetes and dictyostelids are Amoebozoans that are cosmopolitan inhabitants of a variety of habitats, particularly forest environments. Both groups reproduce using spores which are primarily dispersed via wind in myxomycetes but this characteristic poses a problem for dictyostelids. The spores of dictyostelids are incased in a mucilaginous matrix that makes wind ineffective except in exceptional cases. It has been suggested that animals such as birds may play an important yet understudied role in the dispersal of these organisms. This study investigated how animals could potentially serve as vectors for spore dispersal of dictyostelids and myxomycetes with some limited data obtained …

Lichens And Biofilms: Common Collective Growth Imparts Similar Developmental Strategies, Erin C. Carr, Steven D. Harris, Joshua R. Herr, Wayne Riekhof

Department of Plant Pathology: Faculty Publications

Lichens are traditionally defined as a symbiotic relationship between fungi and algae and/or cyanobacteria. This union forms a unique structure called the thallus, which attaches to surfaces such as rocks and tree bark. Recent reports challenge the view that lichens are comprised of one fungus and one photobiont, and instead suggest that they are a consortium of microbes. Much of lichen biology remains unknown as most of our knowledge of lichens is limited to morphological characteristics with little to no functional analysis of lichen genes. However, lichens and biofilms share many similar physiological traits which when compared may assist in …

Assessment Of Grain Safety In Developing Nations, Jose R. Mendoza

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Grains are the most widely consumed foods worldwide, with maize (Zea mays) being frequently consumed in developing countries where it feeds approximately 900 million people under the poverty line of 2 USD per day. While grain handling practices are acceptable in most developed nations, many developing nations still face challenges such as inadequate field management, drying, and storage. Faulty grain handling along with unavoidably humid climates result in recurrent fungal growth and spoilage, which compromises both the end-quality and safety of the harvest. This becomes particularly problematic where there is little awareness about health risks associated with poor …

Spatio-Temporal Dynamics Of Fungal Communities And Their Effects On Plants, Kel Cook

Biology ETDs

Fungi perform several critical functions in the environment. Spatiotemporal distributions of fungal communities will mediate when and where these functions happen and how they vary across the landscape. I first explored tropical tree canopy fungal community variation at small spatial scales and documented near total turnover of fungi across sub-meter distances and among adjacent substrates. The second chapter analyzed fungal turnover over the course of three years, where community stability was driven primarily by abundant fungi. In the third chapter, I tested effects of the environment, including host plant and habitat, on canopy fungal communities and found only small effects, …

Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal

Graduate Theses and Dissertations

Rice blast disease, caused by the filamentous fungus Magnaporthe oryzae, destroys sufficient rice each year to feed 60 million people, and is a serious threat to global food security. A wheat-adapted lineage of M. oryzae now poses threat to global wheat production. Rice blast disease is currently controlled using limited fungicides, and the emergence of fungicide resistance within M. oryzae populations is a growing concern. There is a pressing need to identify new classes of fungicides to control the disease, which requires better understanding of the basic biology of the pathogen. To establish disease, M. oryzae forms a specialized dome …

Investigation Of An Elevational Gradient Reveals Strong Differences Between Bacterial And Eukaryotic Communities Coinhabiting Nepenthes Phytotelmata, Kadeem J. Gilbert, Leonora S. Bittleston, Mark Arcebal K. Naive, Anthony E. Kiszewski, Perry Archival C. Buenavente, David J. Lohman, Naomi E. Pierce

Publications and Research

Elevation is an important determinant of ecological community composition. It integrates several abiotic features and leads to strong, repeatable patterns of community structure, including changes in the abundance and richness of numerous taxa. However, the influence of elevational gradients on microbes is understudied relative to plants and animals. To compare the influence of elevation on multiple taxa simultaneously, we sampled phytotelm communities within a tropical pitcher plant (Nepenthes mindanaoensis) along a gradient from 400 to 1200 m a.s.l. We use a combination of metabarcoding and physical counts to assess diversity and richness of bacteria, micro-eukaryotes, and arthropods, and compare the …

Management Of Storage Pest And Pathogens Of Oat Seeds Using Low Input And Ecofriendly Methods, D. Bahukhandi, D. Vijay, Vikas Kumar, Harsh Vardhan Singh

IGC Proceedings (1997-2023)

Indigenous Traditional knowledge (ITK) is the local knowledge unique to a given culture or society. It is the basis for local-level decision making in agriculture, health care, food preparation, natural-resource management and a host of other activities in rural communities. Storage structures and locally available plant products were used to store seeds and to ward off seed pests and pathogens. In different parts of India, different types of containers are used locally to store different crop seeds, according to the availability and climatic conditions. Sundaramari et. al. (2011) advocated the importance of indigenous storage structures for safe storage of grains …

Population Of Fungi Associated With Seed Germination After Storage And Seed Treatment, Alaba O. Jolaosho, Adijat Olabisi Atayese, Olufunmilayo Comfort Amed, Bolanle Temitope Akinyemi, Jimoh A. Olanite, Victoria O. A. Ojo

IGC Proceedings (1997-2023)

Fungi growth occurs in seeds as a result of environmental conditions (high relative humidity, suitable temperature and high level of seed moisture content). But pathogen free healthy seeds are required for plant propagation. Microbial and insect attack affect seeds throughout the life cycle of the plant (Islam et al., 2009). Fungi are the most common microbes that affect seed germination and they can be both saprophytic and pathogenic. Hence, the aim of this experiment is to determine the cultural and morphological characteristics and population of fungi associated with the germination of some legume seeds after storage and scarification.

Chemical Composition And Fermentation Profile Of Perennial Peanut And Marandu Grass Mixed Silages, Karina G. Ribeiro, Françoise M. Gomes, Odilon G. Pereira, Sebastião De C. Valadares Filho, Thiago C. Da Silva, Vanessa P. Da Silva, Regina S. Santos, Mariele C. N. Agarussi

IGC Proceedings (1997-2023)

Perennial peanut has high quality, evidenced by the improvement of animal production in grazing, due to good contents of crude protein and digestibility, which makes it one of the best alternatives for low cost feeding (Paganella and Valls 2002). Grass ensilage associated with legumes is considered an alternative to meet the protein demand of cattle in the livestock. However, due to limited information on the techniques of grass ensilage with tropical legumes, this research aimed to evaluate the chemical composition and the fermentation profile of perennial peanut and Marandu mixed grass silages, treated or not with bacterial inoculant.

Genetic Characterization And Genome-Wide Association Mapping For Dwarf Bunt Resistance In Bread Wheat Accessions From The Usda National Small Grains Collection, Tyler Gordon, Rui Wang, David Hole, Harold Bockelman, J. Michael Bonman, Jianli Chen

Plants, Soils, and Climate Faculty Publications

Key message

Dwarf bunt-resistant bread wheat accessions and SNP markers associated with DB resistance identified in this study are valuable resources for characterization and deployment of DB resistance in bread wheat.

Abstract

Dwarf bunt (DB), caused by Tilletia controversa J.G. Kühn, can significantly reduce grain yield and quality on autumn-sown wheat in regions with prolonged snow cover. DB can be managed with the use of resistant cultivars. The objectives of the present study were to characterize DB resistance in a large set of bread wheat accessions from the National Small Grains Collection and use a genome-wide association study approach to …

A Preliminary Checklist Of Lichens From Kamiak Butte County Park, Washington State, Emma Sell, Amanda Chandler

2020 Symposium Posters

The semiarid Palouse ecoregion of North America was once dominated by temperate prairies and connects areas of southeast Washington, north-central Idaho, and northeast Oregon. Transformation of nearly all habitats comprising this ecosystem into agricultural lands has drastically reduced extant native grasslands to small, highly fragmented pieces. These land conversion practices, coupled with the subsequent takeover of invasive plant species, have placed the Palouse ecoregion among the most critically endangered ecosystems in the United States, with < 1% of land remaining that is suitable to host native species. The enormous loss of biodiversity across the Palouse has prompted a need for further study regarding a wide variety of organisms. In particular, lichen diversity of the Palouse ecoregion has never been formally characterized, and there are overall very few detailed studies of lichens throughout eastern Washington. To improve knowledge of Palouse lichen diversity, we first reviewed collection data from historical herbarium specimens via the Consortium of North American Lichen Herbaria (CNALH) database. We then collected lichen voucher specimens from Kamiak Butte County Park (KBCP), one of the largest contiguous natural areas within the Palouse ecoregion as its relatively steep, rocky slopes make it ill-suited for agricultural conversion. KBCP consists of 298 acres of mixed Pinus ponderosa (ponderosa pine) and Pseudotsuga menziesii (Douglas fir) forest and native grassland that rises above the surrounding farmland, as well as an east-west ridgeline …

Genetic And Biochemical Characterization Of Ergot Alkaloid Synthesizing Fungi And Their Symbionts, Matthew Duane Maust

Graduate Theses, Dissertations, and Problem Reports

Ergot alkaloids are fungal tryptophan derived toxins which affect mammalian circulation and neurotransmission. These compounds are biosynthesized by a conserved genetic pathway, known as the ergot alkaloid synthesis (EAS) pathway by fungi belonging to the ascomycete families Trichocomaceae and Clavicipitaceae. Several Ipomoea species and related plants in the morning glory family harbor vertically transmitted symbiotic fungi in the genus Periglandula, also members of Clavicipitaceae, that produce ergot alkaloids. Metabolomic analysis of seeds identified a previously uncharacterized glycoside form of the pharmaceutically important ergot alkaloid, ergonovine. Several species belonging to the fungal genus Metarhizium have recently been shown to have the …

Response Of Sorghum Enhanced In Monolignol Biosynthesis To Stalk Rot Pathogens, Deanna L. Funnell-Harris, Scott E. Sattler, Patrick M. O'Neill, Tammy Gries, Hannah M. Tetreault, Thomas E. Clemente

Department of Plant Pathology: Faculty Publications

To increase phenylpropanoid constituents and energy content in the versatile C4 grass sorghum (Sorghum bicolor [L.] Moench), sorghum genes for protiens related to monolignol biosynthesis were overexpressed: SbMyb60 (transcriptional activator), SbPAL (phenylalanine ammonia ase), Bmr2 (4-coumarate: CoA ligase), and SbC3H (coumaroyl shikimate 3-hydroxylase). Overexpression lines were evaluted for responses to stalk pahtogens under greenhouse and field conditions. Greenhouse-grown plants were inoculated with Fusarium thapsinum (Fusarium stalk rot) and Macrophomia phaseolina (charocal rot), which cause yield-reducing diseases. F. thapsinum-inoculated overexpression plants had mean lesion lengths not significantly different than wild-type, except for significantly smaller lesions on two of three SbMyb60 and …