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The Stability Of Epigenetic Variants That Can Act As Loci Causing Phenotypic Change, Raul Faburrieta
The Stability Of Epigenetic Variants That Can Act As Loci Causing Phenotypic Change, Raul Faburrieta
Biology Theses
Epigenetic variations are a possible source of heritable phenotypic variation. In this study I focus on phenotypic alterations seen in epigenetic Recombinant Inbred Lines (epiRILs) of Arabidopsis thaliana. These epiRILs allow me to study the effects differentially methylated regions (DMRs) have on phenotypic variance. In a study performed in 2014 by Cortijo et al., they found that DMR’s affect flowering time and root length when grown under greenhouse conditions. In this study, I replicated the Cortijo et al. (2014) study, with some changes, to see whether the same significant eQTL regions are found. I found that, some of the eQTLs …
Investigating The Expression Of Growth-Regulating Factor 2 (Grf2) In Arabidopsis Thaliana, Sophia S. Henry
Investigating The Expression Of Growth-Regulating Factor 2 (Grf2) In Arabidopsis Thaliana, Sophia S. Henry
Senior Theses
Arabidopsis thaliana is a model organism that is used in laboratories across the world for the study of cellular and molecular processes in plants. GROWTH-REGULATING FACTOR 2 (GRF2) is a transcription factor that promotes leaf growth in Arabidopsis. It is a member of a family of transcription factors that is conserved across all land plants. Previous studies have characterized GRF2 expression in leaves, but GRF2 expression in floral organs has not been studied as extensively. GRF2 acts redundantly with other members of the GRF transcription factor family during floral development. grf1/2/3/5 quadruple mutants show malformed gynoecia, severe fusions between floral …
Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet
Beta-Carbonic Anhydrase Gene Expression Levels Change Depending On The Drought Severity In Both The Leaves And Roots Of Arabidopsis Thaliana, Sertan Çevi̇k, Oğuzhan Kurt, Ayşi̇n Güzel Değer, Ferit Kocaçinar, Rıza Bi̇nzet
Turkish Journal of Botany
In this study, it was aimed to determine the relationship between β carbonic anhydrase (βCA) gene expression levels and drought stress severity. For this purpose, the expression levels of six βCA genes in the roots and leaves of Arabidopsis thaliana were investigated under mild, moderate, and severe drought conditions. In addition, changes in the biomass, leaf water content, photosynthetic efficiency, CA enzyme activity, free proline content, and lipid peroxidation were also determined. The results showed that all of the β CA gene expression levels and CA enzyme activity increased in the leaf under mild drought conditions; however, the expression levels …
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Research in Biology
The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …
The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya
The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya
LSU Doctoral Dissertations
Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO2 and HCO3-. In Arabidopsis thaliana, there are eight αCA genes, six βCA genes, three γCA genes, and two γCA-like genes. The majority of CA research in plants has focused on finding a link between CA activity and photosynthesis rates. Since the CA genes are expressed in different plant tissues and multiple CA isoforms are distributed among various organelles of the plant cell, I hypothesize that CAs facilitate CO2 diffusion among cell compartments and maintain Ci pools for carbon-requiring reactions by interconverting …
Boron Toxicity Induces Sulfate Transporters At Transcriptional Level In Arabidopsis Thaliana, Ceyhun Kayihan, Emre Aksoy, Su Naz Mutlu
Boron Toxicity Induces Sulfate Transporters At Transcriptional Level In Arabidopsis Thaliana, Ceyhun Kayihan, Emre Aksoy, Su Naz Mutlu
Turkish Journal of Botany
Plants activate glutathione (GSH)-dependent detoxification pathways at biochemical and molecular levels under boron (B) toxicity. Sulfate uptake and transport are necessary for GSH biosynthesis in plants. Therefore, the transcriptional regulation of some sulfate transporters was determined in this study to clarify the importance of these transporters in leaf and root tissues of Arabidopsis thaliana under toxic B conditions. The expression level of SULTR1;3 was dramatically increased in leaf and root tissues under moderate and severe toxic B conditions, suggesting source-to-sink sulfate translocation under B toxicity. Stable expression levels of SULTR2;1, SULTR2;2, and low SULTR3;5 expression might restrict the sulfate movement …
The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey
The Effects Of Deleting Lrb E3 Ligase Region 2 On Red Light Responses In Arabidopsis Thaliana, Sabina Liskey
Masters Theses
The ability of plants to absorb light through photoreceptors is essential for successful plant development. Phytochromes are important plant photoreceptors that detect and absorb red and far-red light and must function properly for plant survival. The Light Response BTB proteins (LRB) play an important role in a plant’s response to red light by degrading phytochrome B (PhyB) via the ubiquitin proteasome system (UPS). Although there has been a successful investigation into understanding the function of the LRBs, the function of a few regions within LRB proteins remains unknown, including a portion located on the N-terminal end of the protein, termed …
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Department of Agronomy and Horticulture: Faculty Publications
Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Cell-Type-Specific Profiling Of The Arabidopsis Thaliana Membrane Protein-Encoding Genes, Sergio Alan Cervantes-Pérez, Marc Libault
Department of Agronomy and Horticulture: Faculty Publications
Membrane proteins work in large complexes to perceive and transduce external signals and to trigger a cellular response leading to the adaptation of the cells to their environment. Biochemical assays have been extensively used to reveal the interaction between membrane proteins. However, such analyses do not reveal the unique and complex composition of the membrane proteins of the different plant cell types. Here, we conducted a comprehensive analysis of the expression of Arabidopsis membrane proteins in the different cell types composing the root. Specifically, we analyzed the expression of genes encoding membrane proteins interacting in large complexes. We found that …
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Isolation Of Arabidopsis Thaliana Plants Homozygous For An Insertional Inactivation Mutation Within Atprp4., Sydney Raitz, Timothy D. Trott
Faculty Works
The AtPRP4 gene in Arabidopsis thaliana has been shown to function in several specific parts of the plant’s cell wall. It is shown to be expressed in the seeds, radicles, roots, leaves, inflorescences, and embryos of Arabidopsis thaliana. These patterns have suggested unique functions for ATPRP4 in determining cell-type-specific wall structure during the development of a plant as well as contributing to defense reactions against physical damage to the plant and pathogen infection within the plant. In this study, a simple DNA prep was performed on the true leaves of Arabidopsis thaliana. Subsequent PCR reactions were performed using …
Application Of Synthetic Peptide Cep1 Increases Nutrient Uptake Rates Along Plant Roots, Sonali Roy, Marcus Griffiths, Ivone Torres-Jerez, Bailey Sanchez, Elizabeth Antonelli, Divya Jain, Nicholas Krom, Shulan Zhang, Larry M. York, Wolf-Rüdiger Scheible, Michael Udvardi
Application Of Synthetic Peptide Cep1 Increases Nutrient Uptake Rates Along Plant Roots, Sonali Roy, Marcus Griffiths, Ivone Torres-Jerez, Bailey Sanchez, Elizabeth Antonelli, Divya Jain, Nicholas Krom, Shulan Zhang, Larry M. York, Wolf-Rüdiger Scheible, Michael Udvardi
Agricultural and Environmental Sciences Faculty Research
The root system of a plant provides vital functions including resource uptake, storage, and anchorage in soil. The uptake of macro-nutrients like nitrogen (N), phosphorus (P), potassium (K), and sulphur (S) from the soil is critical for plant growth and development. Small signaling peptide (SSP) hormones are best known as potent regulators of plant growth and development with a few also known to have specialized roles in macronutrient utilization. Here we describe a high throughput phenotyping platform for testing SSP effects on root uptake of multiple nutrients. The SSP, CEP1 (C-TERMINALLY ENCODED PEPTIDE) enhanced nitrate uptake rate per unit root …
Involvement Of Poly(Adp-Ribose)Ylation (Parylation) In The Regulation Of Antioxidant Defense System In Arabidopsis Thaliana Under Salt Stress, Rengi̇n Özgür Uzi̇lday
Involvement Of Poly(Adp-Ribose)Ylation (Parylation) In The Regulation Of Antioxidant Defense System In Arabidopsis Thaliana Under Salt Stress, Rengi̇n Özgür Uzi̇lday
Turkish Journal of Botany
Posttranslational modifications (PTM) are one of the first responses of plants to environmental stress and involve changing the location and activity of proteins in the cell. Addition of poly(ADP-ribose) (PAR) to proteins, poly(ADP-ribose)ylation (PARylation), is a posttranslational modification resulting from the binding of ADP-ribose from NAD+ to target proteins by PAR polymerases (PARP). PARylation is involved in many physiological events in plants including abiotic and biotic stress response. The aim of this work was to understand involvement of PARylation in inducing enzymatic antioxidant defence and alternative electron sinks in response to salinity. For this purpose A. thaliana plants were treated …
Formins Control Dynamics Of F-Actin In The Central Cell Of Arabidopsis Thaliana, Mohammad F. Ali, Tomokazu Kawashima
Formins Control Dynamics Of F-Actin In The Central Cell Of Arabidopsis Thaliana, Mohammad F. Ali, Tomokazu Kawashima
Plant and Soil Sciences Faculty Publications
In the female gamete of flowering plants, sperm nuclear migration is controlled by a constant inward movement of actin filaments (F-actin) for successful fertilization. This dynamic F-actin movement is ARP2/3-independent, raising the question of how actin nucleation and polymerization is controlled in the female gamete. Using confocal microscopy live-cell imaging in combination with a pharmacological approach, we assessed the involvement of another group of actin nucleators, formins, in F-actin inward movement in the central cell of Arabidopsis thaliana. We identify that the inhibition of the formin function, by formin inhibitor SMIFH2, significantly reduced the dynamic inward movement of F-actin …
The Influence Of Singlet Oxygen And Loss Of Function Of Fatty Acid Desaturase 7 In The Chloroplast On Aphid Resistance In Arabidopsis Thaliana, Hillary Donna Fischer
The Influence Of Singlet Oxygen And Loss Of Function Of Fatty Acid Desaturase 7 In The Chloroplast On Aphid Resistance In Arabidopsis Thaliana, Hillary Donna Fischer
Graduate Theses and Dissertations
Fatty Acid Desaturase 7 (FAD7) is a chloroplast-localized enzyme that alters the fatty acid content of photosynthetic membranes, and that negatively regulates plant defenses against aphids. Previous studies in the model organism Arabidopsis (Arabidopsis thaliana) have shown that loss-of-function mutations in FAD7 decrease population growth of the green peach aphid (GPA; Myzus persicae Sulzer). This study further characterized the effects of a fad7 null mutant on aphids, and investigated the role of reactive oxygen species (ROS), including singlet oxygen (1O2), in plant responses to aphid resistance in fad7 as well as in wild type plants and a mutant with heightened …
The Ear Motif In The Arabidopsis Mads Transcription Factor Agamous-Like 15 Is Not Necessary To Promote Somatic Embryogenesis, Sanjay Joshi, Christian Keller, Sharyn E. Perry
The Ear Motif In The Arabidopsis Mads Transcription Factor Agamous-Like 15 Is Not Necessary To Promote Somatic Embryogenesis, Sanjay Joshi, Christian Keller, Sharyn E. Perry
Plant and Soil Sciences Faculty Publications
AGAMOUS-like 15 (AGL15) is a member of the MADS domain family of transcription factors (TFs) that can directly induce and repress target gene expression, and for which promotion of somatic embryogenesis (SE) is positively correlated with accumulation. An ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motif of form LxLxL within the carboxyl-terminal domain of AGL15 was shown to be involved in repression of gene expression. Here, we examine whether AGL15′s ability to repress gene expression is needed to promote SE. While a form of AGL15 where the LxLxL is changed to AxAxA can still promote SE, another form with a …
The Involvement Of The Induction Of Anthocyanin Biosynthesis And Transport In Toxic Boron Responsive Regulation In Arabidopsis Thaliana, Ceyhun Kayihan
The Involvement Of The Induction Of Anthocyanin Biosynthesis And Transport In Toxic Boron Responsive Regulation In Arabidopsis Thaliana, Ceyhun Kayihan
Turkish Journal of Botany
Recently, boron (B) has been found to form a complex with anthocyanin, which could be evidence for the B tolerance mechanisms that reduce free B in the leaf tissues of plants. However, the molecular mechanism of anthocyanin biosynthesis and transport has not been satisfactorily elucidated in plants exposed to toxic B. Therefore, the changes in expression levels of some of the phenylpropanoid pathway genes, early and late flavonoid biosynthetic genes, and transcription factors related to anthocyanin biosynthesis and transport were determined in Arabidopsis thaliana under B toxicity. Accordingly, 1 mM boric acid treatment did not cause any significant change in …
Nmr Spectroscopy Analysis Reveals Differential Metabolic Responses In Arabidopsis Roots And Leaves Treated With A Cytokinesis Inhibitor, Thomas E. Wilkop, Minmin Wang, Angelo Heringer, Jaideep Singh, Florence Zakharov, Viswanathan V. Krishnan, Georgia Drakakaki
Nmr Spectroscopy Analysis Reveals Differential Metabolic Responses In Arabidopsis Roots And Leaves Treated With A Cytokinesis Inhibitor, Thomas E. Wilkop, Minmin Wang, Angelo Heringer, Jaideep Singh, Florence Zakharov, Viswanathan V. Krishnan, Georgia Drakakaki
Physiology Faculty Publications
In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In …
The Effects Of Environmental Stress On Arabidopsis Thaliana Establishment And Development, Paige Petty
The Effects Of Environmental Stress On Arabidopsis Thaliana Establishment And Development, Paige Petty
Student Research, Papers, and Creative Works
Arabidopsis thaliana is a model organism for plant research because it grows quickly, is easy to maintain, and findings from Arabidopsis can be easily extrapolated to crop species such as tomato, soybean, corn, and sorghum. Of particular interest is the process of photomorphogenesis, which promotes seedling establishment in soil. A rise in temperature causes stress for the seedling, which can inhibit photomorphogenesis and cause elongated hypocotyls that grow regardless of light intensity. Drought can also affect the rate of photosynthesis and limit seedling growth. In this project, we explored how the adult Arabidopsis plant is affected by delayed seedling establishment …
Genome-Wide Occupancy Of Polycomb Group Proteins And Chromatin Remodeler Splayed And Their Interplay In Arabidopsis, Jie Shu
Electronic Thesis and Dissertation Repository
The Polycomb group (PcG) proteins form two protein complexes, Polycomb repressive complex 1 (PRC1) and PRC2, which are key epigenetic regulators in eukaryotes. PRC2 represses gene expression by catalyzing trimethylation of histone H3 lysine 27 (H3K27me3). In Arabidopsis thaliana, CURLY LEAF (CLF) and SWINGER (SWN) are two major H3K27 methyltransferases, playing essential roles in plant growth and development. Despite their importance, genome-wide occupancy profiles of CLF and SWN have not yet been determined and compared. In this thesis, I generated transgenic lines expressing GFP-tagged CLF/SWN and determined the genome-wide distributions of CLF and SWN in Arabidopsis seedlings. I also …
Moderate Level Of Toxic Boron Causes Differential Regulation Of Micrornas Related To Jasmonate And Ethylene Metabolisms In Arabidopsis Thaliana, Doğa Seli̇n Kayihan, Ceyhun Kayihan, Yelda Özden Çi̇ftçi̇
Moderate Level Of Toxic Boron Causes Differential Regulation Of Micrornas Related To Jasmonate And Ethylene Metabolisms In Arabidopsis Thaliana, Doğa Seli̇n Kayihan, Ceyhun Kayihan, Yelda Özden Çi̇ftçi̇
Turkish Journal of Botany
Earlier our colleagues detected that the genes related to jasmonate (JA), ethylene, and cell wall modification were significantly regulated under boron (B) toxicity in wheat. Determination of regulation mechanisms of these novel genes under B toxicity is very important in Arabidopsis thaliana as a model plant. As key regulators, the microRNAs (miRNAs) regulate gene expression at the posttranscriptional level and respond to numerous abiotic stresses in plants. In this study, expression levels of miRNAs such as miR159, miR172, miR319, and miR394 targeting JA and ethylene-related transcription factors and also miR397 targeting laccase were determined in Arabidopsis thaliana under toxic B …
Defective Aba-Mediated Sugar Signalling Pathway In An Established Arabidopsis Thaliana Cell Suspension Culture Explains Its Stay-Green Phenotype At High Sugar Concentrations, Avery Mccarthy
Electronic Thesis and Dissertation Repository
An unusual sugar insensitive phenotype was identified in an established cell suspension culture of Arabidopsis thaliana. We characterized the physiology, biochemistry and genetics of the sugar insensitive cell culture, in order to identify factors contributing to the phenotype. Chlorophyll levels of the cell suspension culture were insensitive to high sucrose (6-15% w/v) and maintained a green phenotype. Immunoblotting indicated that levels of key photosynthetic proteins (PsaA, Lhcb2 and Rubisco) increased as a function of external sucrose concentration. The green cell culture was photosynthetically competent based on light-dependent, CO2-saturated rates of O2 evolution as well as Fv/Fm …
Nonpolar Residues In The Presumptive Pore‐Lining Helix Of Mechanosensitive Channel Msl10 Influence Channel Behavior And Establish A Nonconducting Function, Grigory Maksaev, Jennette K. Shoots, Simran Ohri, Elizabeth S. Haswell
Nonpolar Residues In The Presumptive Pore‐Lining Helix Of Mechanosensitive Channel Msl10 Influence Channel Behavior And Establish A Nonconducting Function, Grigory Maksaev, Jennette K. Shoots, Simran Ohri, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Mechanosensitive (MS) ion channels provide a universal mechanism for sensing and responding to increased membrane tension. MscS‐like (MSL) 10 is a relatively well‐studied MS ion channel from Arabidopsis thaliana that is implicated in cell death signaling. The relationship between the amino acid sequence of MSL10 and its conductance, gating tension, and opening and closing kinetics remains unstudied. Here, we identify several nonpolar residues in the presumptive pore‐lining transmembrane helix of MSL10 (TM6) that contribute to these basic channel properties. F553 and I554 are essential for wild type channel conductance and the stability of the open state. G556, a glycine residue …
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase9 Using Yeast 2-Hybridization, Sheikh Hossain
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase9 Using Yeast 2-Hybridization, Sheikh Hossain
Senior Honors Projects, 2010-2019
In plants, starch is a major carbon and energy storage compound. Starch is made as a product of photosynthesis while plants are in light and is degraded at night. Our lab is interested in the cellular mechanism of starch degradation in plants and for our studies we use Arabidopsis thaliana as a model. β-amylases are primarily responsible for the hydrolysis of starch in plants and a total of nine β-amylases genes are encoded in Arabidopsis thaliana. These nine genes are identified as BAM1-9. BAM9 is located in the chloroplast where starch is located, and is present …
Development Of Ifox-Hunting As A Functional Genomic Tool And Demonstration Of Its Use To Identify Early Senescence-Related Genes In The Polyploid Brassica Napus, Juan Ling, Renjie Li, Chinedu Charles Nwafor, Junluo Cheng, Maoteng Li, Qing Xi, Jian Wu, Lu Gan, Qingyong Yang, Chao Liu, Ming Chen, Yongming Zhou, Edgar B. Cahoon, Chunyu Zhang
Development Of Ifox-Hunting As A Functional Genomic Tool And Demonstration Of Its Use To Identify Early Senescence-Related Genes In The Polyploid Brassica Napus, Juan Ling, Renjie Li, Chinedu Charles Nwafor, Junluo Cheng, Maoteng Li, Qing Xi, Jian Wu, Lu Gan, Qingyong Yang, Chao Liu, Ming Chen, Yongming Zhou, Edgar B. Cahoon, Chunyu Zhang
Center for Plant Science Innovation: Faculty and Staff Publications
Functional genomic studies of many polyploid crops, including rapeseed (Brassica napus), are constrained by limited tool sets. Here we report development of a gain-of-function platform, termed ‘iFOX (inducible Full-length cDNA OvereXpressor gene)-Hunting’, for inducible expression of B. napus seed cDNAs in Arabidopsis. A Gateway-compatible plant gene expression vector containing a methoxyfenozide-inducible constitutive promoter for transgene expression was developed. This vector was used for cloning of random cDNAs from developing B. napus seeds and subsequent Agrobacterium-mediated transformation of Arabidopsis. The inducible promoter of this vector enabled identification of genes upon induction that are otherwise lethal when constitutively overexpressed and …
Analysis Of Argonaute-Small Rna-Transcription Factor Circuits Controlling Leaf Development, John Steen Hoyer
Analysis Of Argonaute-Small Rna-Transcription Factor Circuits Controlling Leaf Development, John Steen Hoyer
Arts & Sciences Electronic Theses and Dissertations
Experimental studies of plant development have yielded many insights into gene regulation, revealing interactions between core transcriptional and post-transcriptional regulatory pathways present in all land plants. This work describes a direct connection between the three main small RNA-transcription factor circuits controlling leaf shape dynamics in the reference plant Arabidopsis thaliana. We used a high-throughput yeast 1-hybrid platform to identify factors directly binding the promoter of the highly specialized ARGONAUTE7 silencing factor. Two groups of developmentally significant microRNA-targeted transcription factors were the clearest hits from these screens, but transgenic complementation analysis indicated that their binding sites make only a small contribution …
Non-Canonical Signaling From The Etr1 And Etr2 Ethylene Receptors In Arabidopsis Thaliana, Arkadipta Bakshi
Non-Canonical Signaling From The Etr1 And Etr2 Ethylene Receptors In Arabidopsis Thaliana, Arkadipta Bakshi
Doctoral Dissertations
The gaseous phytohormone ethylene regulates several physiological and developmental processes in higher plants. There are five ethylene receptor isoforms that mediate the responses to ethylene in the model plant Arabidopsis thaliana. Prior research has shown that these five ethylene receptor isoforms in Arabidopsis have both overlapping and non-overlapping roles in regulating diverse responses such as growth in air, growth recovery after removal of ethylene, and ethylene stimulated nutational bending. Functional divergence of ETR1 has been determined in controlling some of these traits and in some of these cases, ETR1 subfunctionalization requires the receiver domain. Using homology modeling and sequence …
Image-Based Analysis To Dissect Vertical Distribution And Horizontal Asymmetry Of Conspecific Root System Interactions In Response To Planting Densities, Nutrients And Root Exudates In Arabidopsis Thaliana, Jane Geisler-Lee, Xian Liu, Wei Rang, Jayanthan Raveendiran, Marisa Blake Szubryt, David John Gibson, Matt Geisler, Qiang Cheng
Image-Based Analysis To Dissect Vertical Distribution And Horizontal Asymmetry Of Conspecific Root System Interactions In Response To Planting Densities, Nutrients And Root Exudates In Arabidopsis Thaliana, Jane Geisler-Lee, Xian Liu, Wei Rang, Jayanthan Raveendiran, Marisa Blake Szubryt, David John Gibson, Matt Geisler, Qiang Cheng
Institute for Biomedical Informatics Faculty Publications
Intraspecific competition is an important plant interaction that has been studied extensively aboveground, but less so belowground, due to the difficulties in accessing the root system experimentally. Recent in vivo and in situ automatic imaging advances help understand root system architecture. In this study, a portable imaging platform and a scalable transplant technique were applied to test intraspecific competition in Arabidopsis thaliana. A single green fluorescent protein labeled plant was placed in the center of a grid of different planting densities of neighboring unlabeled plants or empty spaces, into which different treatments were made to the media. The root …
Selection Of Efficient Taq Dna Polymerase To Optimize T-Dna Genotyping Method For Rapid Detection Of Mutant Arabidopsis Thaliana Plants, Inna B. Chastukhina, Liliia R. Nigmatullina, Lia R. Valeeva, Eugene V. Shakirov
Selection Of Efficient Taq Dna Polymerase To Optimize T-Dna Genotyping Method For Rapid Detection Of Mutant Arabidopsis Thaliana Plants, Inna B. Chastukhina, Liliia R. Nigmatullina, Lia R. Valeeva, Eugene V. Shakirov
Biological Sciences Faculty Research
Plants harbor homologues of various animal genes involved in phosphorus metabolism, telomere biology and other cellular processes. Compared to experiments with many other multicellular organisms, research in the model plant Arabidopsis thaliana takes advantage of short generation time and an ever increasing arsenal of genetic and transgenic tools, including large collections of T-DNA knockout and activation lines. The availability of thousands of publicly available transgenic Arabidopsis lines provides a unique opportunity to address a number of important biological questions. However, identification of individual T-DNA mutant plants from a pool of seeds provided by a biological stock distribution center remains a …
Plastid Osmotic Stress Influences Cell Differentiation At The Plant Shoot Apex, Margaret E. Wilson, Matthew Mixdorf, R Howard Berg, Elizabeth S. Haswell
Plastid Osmotic Stress Influences Cell Differentiation At The Plant Shoot Apex, Margaret E. Wilson, Matthew Mixdorf, R Howard Berg, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
The balance between proliferation and differentiation in the plant shoot apical meristem is controlled by regulatory loops involving the phytohormone cytokinin and stem cell identity genes. Concurrently, cellular differentiation in the developing shoot is coordinated with the environmental and developmental status of plastids within those cells. Here, we employ an Arabidopsis thaliana mutant exhibiting constitutive plastid osmotic stress to investigate the molecular and genetic pathways connecting plastid osmotic stress with cell differentiation at the shoot apex. msl2 msl3 mutants exhibit dramatically enlarged and deformed plastids in the shoot apical meristem, and develop a mass of callus tissue at the shoot …
Glucan Synthase-Like 8: A Key Player In Early Seedling Development In Arabidopsis, Behnaz Saatian
Glucan Synthase-Like 8: A Key Player In Early Seedling Development In Arabidopsis, Behnaz Saatian
Electronic Thesis and Dissertation Repository
Plants’ cell walls have unique chemical composition and features which enable them to play essential roles during plant development as shaping the cells and providing intercellular communication between adjacent cells. Polysaccharides, including callose, and glycoproteins are known as the main constituents of the cell wall. Callose, a linear β-1,3-glucan polymer, is accumulated at the cell plate during cytokinesis, in plasmodesmata, where it regulates cell-to-cell communication, in dormant phloem, where it seals sieve plates after mechanical injury and pathogen attack, and in male and female gametophytes. GLUCAN SYNTHASE-LIKE (GSL) genes in Arabidopsis comprise a family of 12 members. A …