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Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma
Hairy Roots As A Model To Investigate The Role Of Suberin In The Phytophthora Sojae-Soybean Pathosystem, Pooja Sharma
Electronic Thesis and Dissertation Repository
Part of the resistance mechanism of soybean (Glycine max [L.] Merr.) to Phytopthora sojae Kauf. & Gerd. involves pre-formed root suberin. In order to investigate the role of suberin in this host-pathogen interaction, I characterized hairy roots, formed as a result of Agrobacterium rhizogenes (Riker et al.) Conn infection, as a model to be used as a reliable soybean transformation system. I established hairy root cultures and demonstrated that they were a result of A. rhizogenes infection. The anatomy and suberin deposition in soybean hairy roots was examined, and found to be very similar to that of wild-type roots. …
Effects Of Environment And Genotype On Charcoal Rot Development On Soybeans, Micah Diane Doubledee
Effects Of Environment And Genotype On Charcoal Rot Development On Soybeans, Micah Diane Doubledee
Graduate Theses and Dissertations
Charcoal rot of soybean, caused by the soilborne fungus Macrophomina phaseolina (Tassi) Goid, is a disease associated with high soil temperature and low soil moisture. Above-ground symptoms, which can be difficult to distinguish from drought symptoms, include low vigor, dead leaves that remain attached to the plant, early senescence and yield loss. Irrigation limits damage, but does not prevent colonization of the tissue by the pathogen. No soybean line is immune to M. phaseolina, but a few lines may have moderate resistance. The objectives of this research were to 1) determine the effects of genotype and drought on the development …
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
Molecular Approaches To Detect And Control Cercospora Kikuchii In Soybeans, Ashok Kumar Chanda
LSU Doctoral Dissertations
Cercospora leaf blight (CLB) caused by Cercospora kikuchii, has become a troublesome disease in the southern United States. C. kikuchii produces a non-hostspecific phytotoxin and a pathogenicity factor known as cercosporin during infection of soybean. A quantitative real-time PCR assay was developed for detection and quantification of C. kikuchii. The sensitivity of detection is 1 pg of genomic DNA. The assay detected the presence of C. kikuchii in soybean leaves long before the appearance of disease symptoms. C. kikuchii DNA levels in soybean leaves increased slowly during early soybean development, followed by a quick increase at late reproductive stages. Results …