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A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
A Polymerase Chain Reaction Method For Identification Of Five Major Meloidogyne Species, Thomas O. Powers, T. S. Harris
Department of Plant Pathology: Faculty Publications
A polymerase chain reaction (PCR) method for discriminating Meloidogyne incognita, M. arenaria, M. javanica, M. hapla, and M. chitwoodi was developed. Single juveniles were ruptured in a drop of water and added directly to a PCR reaction mixture in a microcentrifuge tube. Primer annealing sites were located in the 3' portion of the mitochondrial gene coding for cytochrome oxidase subunit II and in the 16S rRNA gene. Following PCR amplification, fragments of three sizes were detected. The M. incognita and M. javanica reactions produced a 1.7-kb fragment; the M. arenaria reaction, a 1.1-kb fragment; and the …
Mitochondrial Dna Sequence Divergence Among Meloidogyne Incognita, Romanomermis Culicivorax, Ascaris Suum, And Caenorhabditis Elegans, Thomas O. Powers, T. S. Harris, B. C. Hyman
Mitochondrial Dna Sequence Divergence Among Meloidogyne Incognita, Romanomermis Culicivorax, Ascaris Suum, And Caenorhabditis Elegans, Thomas O. Powers, T. S. Harris, B. C. Hyman
Department of Plant Pathology: Faculty Publications
Mitochondrial DNA sequences were obtained from the NADH dehydrogenase subunit 3 (ND3), large rRNA, and cytochrome b genes from Meloidogyne incognita and Romanomermis culicivorax. Both species show considerable genetic distance within these same genes when compared with Caenorhabditis elegans or Ascaris suum, two species previously analyzed. Caenorhabditis, Ascaris, and Meloidogyne were selected as representatives of three subclasses in the nematode class Secernentea: Rhabditia, Spiruria, and Diplogasteria, respectively. Romanomermis served as a representative outgroup of the class Adenophorea. The divergence between the phytoparasitic lineage (represented by Meloidogyne) and the three other species is so great that virtually …