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Molecular Identification Of Oomycete Species Associated With Woody Plants In Louisiana And Survey Of Oomycete Species Associated With Live Oak Trees Planted On The Louisiana State University Campus, Hamilton Crockett
LSU Master's Theses
Three Oomycetes genera, Phytophthora, Phytopythium and Pythium, contain many important plant pathogens. Historically, Phytophthora infestans, responsible for the great Irish potato famine, and Phytophthora ramorum, causal agent of sudden oak death disease, are the two better-known high impact pathogens. The LSU AgCenter Plant Diagnostic Center (PDC) has been isolating putative Phytophthora and related species from combined root and soil samples collected from various woody ornamentals and trees for over a decade. In August of 2016, Louisiana received torrential rains, which resulted in historic flooding in several southern parishes. During subsequent years, the PDC staff isolated a …
Genetic Analysis Of Field Populations Of The Plant Pathogens Cercospora Sojina, Corynespora Cassiicola And Phytophthora Colocasiae, Sandesh Kumar Shrestha
Genetic Analysis Of Field Populations Of The Plant Pathogens Cercospora Sojina, Corynespora Cassiicola And Phytophthora Colocasiae, Sandesh Kumar Shrestha
Doctoral Dissertations
Genetic markers and whole genome sequencing (WGS) were used to study the disease dynamics and population structure for three important plant pathogens; Cercospora sojina (frogeye leaf spot of soybean), Corynespora cassiicola (target spot of soybean, cotton and many other crops) and Phytophthora colocasiae (taro leaf blight). For each pathogen, genome sequencing was used to guide the development of single nucleotide polymorphism (SNP) markers and both were used to investigate diversity in field populations. Investigation of C. sojina in Tennessee included comparisons of extant populations and historical isolates, revealing a dominant, potentially long-lived, clonal lineage. Characterization of QoI fungicide resistance indicates …
Phytophthora Sojae Infecting Soybean: Pathotype Diversity, New Sources Of Resistance And Interaction With The Soybean Cyst Nematode, Rawnaq Nazneen Chowdhury
Phytophthora Sojae Infecting Soybean: Pathotype Diversity, New Sources Of Resistance And Interaction With The Soybean Cyst Nematode, Rawnaq Nazneen Chowdhury
Electronic Theses and Dissertations
Phytophthora root and stem rot, caused by Phytophthora sojae Kaufmann and Gerdemann, is an important disease of soybean (Glycine max L.) in South Dakota. To gain a better understanding of the importance of P. sojae in South Dakota, specifically pathotype diversity, identification of new resistance sources and the interaction with the soybean cyst nematode (Heterodera glycines Ichinohe, SCN), this research was undertaken with the following objectives - 1) to characterize the pathotype diversity of P. sojae causing Phytophthora root and stem rot on soybean in commercial fields in South Dakota; 2) to compare inoculation methods to evaluate for …
Characterization Of A Novel Clade Of Transporters In Phytophthora, Stephanie Padula, Paul F. Morris Dr, Howard Casey Cromwell Dr., Menaka Ariyaratne, Andrew Wagner
Characterization Of A Novel Clade Of Transporters In Phytophthora, Stephanie Padula, Paul F. Morris Dr, Howard Casey Cromwell Dr., Menaka Ariyaratne, Andrew Wagner
Honors Projects
The oomycete Phytophthora parasitica has a worldwide distribution and is an economically important pathogen of more than 100 species4. RNA-seq analysis showed that one gene, PPTG_16698 has the 5th highest level of expression of all transport proteins in the zoospore stage, and is highly conserved throughout Phytophthora species. This project attempts to characterize the important biological role that PPTG_16698 plays in P. parasitica and other oomycetes. Three strategies have been implemented to accomplish this goal: growth analysis by heterologous expression in yeast, metabolite analysis in yeast, and construction of a GFP fusion protein to enable localization of …
Phytophthora Foliage Blight Of Garden Phlox: Phytophthora Nicotianae On Phlox Paniculata, Daniel Drechsler
Phytophthora Foliage Blight Of Garden Phlox: Phytophthora Nicotianae On Phlox Paniculata, Daniel Drechsler
All Theses
Phytophthora foliage blight of garden phlox (Phlox paniculata ) - caused by Phytophthora nicotianae - is a devastating disease that has been seen consistently in nursery production facilities in South Carolina. However, this disease has not been characterized previously. This project was composed of three separate studies, each of which followed one of the three major components of the disease triangle (i.e., the synchronous occurrence of a susceptible host and a virulent pathogen within a conducive environment) to investigate the pathogenicity of P. nicotianae on P. paniculata . In one study, the efficacies of three inoculation methodologies to cause …
Diversity Among Isolates Of Phytophthora Cinnamomi From Ornamental Plants In South Carolina, Simon Schreier
Diversity Among Isolates Of Phytophthora Cinnamomi From Ornamental Plants In South Carolina, Simon Schreier
All Theses
Phytophthora cinnamomi is a devastating pathogen that can attack over 900 hosts. It is the most common species of Phytophthora isolated from woody ornamental crops in South Carolina but little is known about variability among isolates of P. cinnamomi that attack these plants. Therefore, 142 isolates of P. cinnamomi recovered from diseased plant samples submitted to the Clemson University Plant Problem Clinic between 1995 and 2011 were characterized for mycelium growth habit, growth rate, mefenoxam sensitivity, mating type, and sporangium morphology. Mycelium growth habit on PARPH-V8 selective medium was classified as aerial, appressed, or dwarf; 87% of isolates had the …
Evaluation Of Wetland Plants For Susceptibility To Species Of Phytophthora Present In Runoff Water At Ornamental Plant Nurseries, Garrett Ridge
Evaluation Of Wetland Plants For Susceptibility To Species Of Phytophthora Present In Runoff Water At Ornamental Plant Nurseries, Garrett Ridge
All Theses
Competition between agricultural producers and urban communities for high quality water is increasing; therefore, growers need to adopt water conservation and reuse practices to ensure an adequate supply of irrigation water. One concern with recycled irrigation water is the potential to recirculate propagules of oomycete pathogens. Constructed wetlands are a biologically-based treatment option for nutrient and chemical remediation that potentially also could filter pathogen propagules. The goal of this study was to assess the susceptibility of plants routinely used in constructed wetlands to infection by species of Phytophthora commonly found at ornamental plant nurseries. In laboratory and greenhouse experiments, an …
Diseases Of Floriculture Crops In South Carolina: Evaluation Of A Pre-Plant Sanitation Treatment And Identification Of Species Of Phytophthora, Ernesto Robayo Camacho
Diseases Of Floriculture Crops In South Carolina: Evaluation Of A Pre-Plant Sanitation Treatment And Identification Of Species Of Phytophthora, Ernesto Robayo Camacho
All Theses
This project was composed of two separate studies. In one study, the species of Phytophthora that have been found associated with diseased floriculture crops in South Carolina and four other states were characterized and identified using molecular (RFLP fingerprints and DNA sequences for ITS regions and cox I and II genes), morphological (sporangia, oogonia, antheridia, oospores, and chlamydospores), and physiological (mating behavior and cardinal temperatures) characters. In addition, sensitivity to the fungicide mefenoxam was determined. In all, 87 isolates from 63 host plants that were collected over a 13-year period (1996 through 2008) were examined. Host plants came from 46 …