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Morphological And Physio-Biochemical Responses And Gene Expression Analyses Of Landscape Plants Under Salinity Stress, Asmita Paudel

All Graduate Theses and Dissertations, Fall 2023 to Present

Soil salinity is a significant global issue that adversely impacts the growth and development of landscape plants. One of the effective strategies to prevent salinity damage to landscape plants is to cultivate species that are tolerant to the prevailing salinity levels. Salinity tolerance varies among plant species and cultivars. Therefore, this research aimed to investigate the salinity tolerance of nine landscape plants [Albizia julibrissin (mimosa tree), Arctostaphylos uva-ursi (kinnikinnick), Cercocarpus ledifolius (curl-leaf mountain mahogany), Cercocarpus montanus ‘Coy’ (alder-leaf mountain mahogany), Penstemon barbatus ‘Novapenblu’ (rock candy blue^® penstemon), Penstemon strictus ‘Rocky Mountain’ (rocky mountain beardtongue), Punica granatum ‘Wonderful’ (pomegranate), …

Investigating The Impacts Of Drought On Turfgrass (Festuca Arundinacea) Chlorophyll-A Fluorescence Emission, Ayanna S. Hatton

Honors Projects

When photons from sunlight are absorbed by plants, they can take paths of photosynthesis, fluorescence, or energy dissipation. Instruments to quantify fluorescence have expanded in scale to allow measurements from satellites and flux towers using Solar-Induced Chlorophyll Fluorescence (SIF). Studies have found a positive correlation between SIF and gross primary productivity (GPP; representative of photosynthesis), suggesting SIF is a proxy for GPP. This conclusion encourages the use of SIF to inform decisions about carbon budgets and responding to climate change. Studies of fluorescence on the single-leaf scale have revealed that SIF measurements do not account for all variables nor is …

Mimicking Biofilms: Photosynthetic Assessments Of C. Reinhardtii In 3 Physical Forms, John Michael Roesgen

Biology ETDs

Oxygenic photosynthesis supports the majority of life on Earth through the capture of energy from sunlight and the assimilation of CO₂ into basic building blocks of cells. Microalgae are fast growing and account for about half of global photosynthesis. In addition, they can be cultivated and their metabolism can be redirected to generate additional useful products ranging from biofuels to pharmaceuticals. However, the efficiency of metabolite production is severely impacted by the slow diffusion of CO₂ through water and the high energetic costs of harvesting microalgae from liquid cultures. Microalgae grow in open water, but they also form …

The Characterization Of Selected Α- And Β-Carbonic Anhydrases Of Arabidopsis Thaliana, Hiruni Weerasooriya

LSU Doctoral Dissertations

Carbonic anhydrases (CAs) are zinc-metalloenzymes that interconvert two inorganic carbon (Ci) species, CO₂ and HCO₃^-. In Arabidopsis thaliana, there are eight αCA genes, six βCA genes, three γCA genes, and two γCA-like genes. The majority of CA research in plants has focused on finding a link between CA activity and photosynthesis rates. Since the CA genes are expressed in different plant tissues and multiple CA isoforms are distributed among various organelles of the plant cell, I hypothesize that CAs facilitate CO₂ diffusion among cell compartments and maintain Ci pools for carbon-requiring reactions by interconverting …

Propagation, Physiology And Biomass Of Giant Cane (Arundinaria Gigantea) For Conservation And Restoration, Sanjeev Sharm

MSU Graduate Theses

Giant cane (Arundinaria gigantea) is a native species to 22 states in the U.S. The species and its ecosystem are considered critically endangered, and the species has been reduced to 2% of its original extent. The species has a long cultural and conservation history. Large canebrakes were commonly found in Missouri in bottomland forests, stream and riverbanks, and margins of lakes. My research goals were to: 1) examine methods for propagation success from field to greenhouse to field; 2) examine the physiology of cane at one of the few current canebrakes, for greenhouse propagated plants, and field planted …

Evaluating The Impacts Of Waterlogging Stress On Cowpea (Vigna Unguiculata L.) Growth Traits And Physiological Performance, Omolayo Joshua Olorunwa

Theses and Dissertations

The progressive increase in the global population and the rapidly changing climate have put unprecedented pressure on crop production. Cowpea is one of the world’s most important leguminous crops, contributing to food security and environmental sustainability. However, cowpea productivity is limited due to waterlogging stress. The main objective of this study was to explore physiological and biochemical mechanisms to understand how cowpea genotypes respond to waterlogging stress. Four studies were conducted in controlled and field conditions to achieve these objectives. Study 1 characterized the waterlogging tolerance of 30 cowpea genotypes in a controlled environment using 24 morphophysiological parameters with waterlogging …

Loblolly Pine (Pinus Taeda) Leaf-Level Physiology And The Influence Of Genotype, Drought, And Thinning, Norman Jesse Cone Iv

Master's Theses

The Southeastern U.S. is known as the “Wood Basket” for producing the vast majority of U.S. timber, but projected increases in the frequency of severe drought events could threaten timber plantations. Loblolly pine (Pinus taeda) is the principle timber species of the southeast and is distributed across the Atlantic Coastal Plains and west into Texas. Western Gulf states (LA, TX, AR, and OK) lie at the edge of the range for loblolly pine and are particularly vulnerable to any changes in precipitation or temperature. Current research suggests two main methods to increase plantation drought resistance: plant drought resistant seedlings or …

Phenotypic Responses Of Sagebrush To The Southwestern North America Megadrought: A Genotype-By-Environment (Gxe) Approach, Kara A. Navock

Boise State University Theses and Dissertations

The Southwestern North America megadrought is an extreme climate event. Artemisia tridentata (big sagebrush) is the dominant, keystone species of sagebrush-steppe ecosystems in arid and semi-arid habitats of western North America. I conducted a genotype-by-environment (GxE) experiment on two putative genotypes (drought-tolerant, G1 and drought-sensitive, G2) and two cytotypes, diploid (2x) and tetraploid (4x), to determine the phenotypic responses of big sagebrush seedlings to drought. For three chlorophyll fluorescence parameters, my results indicate a complex set of factors influence sagebrush responses to drought, including canalization, adaptive phenotypic plasticity, cryptic genetic diversity, and GxE interactions. Variation in leaf temperature profiles of …

Can Desert Mosses Hide From Climate Change? The Ecophysiological Importance Of Habitat Buffering & Water Relations To A Keystone Biocrust Moss In The Mojave Desert, Theresa Ann Clark

UNLV Theses, Dissertations, Professional Papers, and Capstones

Empirical and observational studies suggest a keystone biocrust moss, Syntrichia caninervis, may be sensitive to future climate change in the American Southwest due to its uniquely sensitive water relations that appear particularly challenged during summer hydration-desiccation cycles. However, the potential mitigating roles of habitat buffering, acclimatization, and winter recovery on the vulnerability of this species remain largely unexplored. I investigated potential abiotic and biotic resiliency factors driving summer stress resistance and recovery in S. caninervis along present-day aridity gradients in the Mojave Desert to strengthen the climate change vulnerability assessment for this species common to biocrusts of North America, northern …

Effects Of Elevated Temperature And Elevated Co2 On Leaf Carbon Fluxes In Boreal Conifers: Lab And Field Studies, Mirindi Eric Dusenge

Electronic Thesis and Dissertation Repository

Rising CO₂ may warm northern latitudes up to 10 °C by the end of the century. However, responses of plant physiological processes (such as photosynthesis and respiration) and growth to climate change remain uncertain. Seedlings and mature trees of tamarack (a deciduous species) and black spruce (an evergreen species), North America dominant conifers, were exposed to combined warming (up to +9 ˚C) and elevated CO₂ (up to +300 ppm). In seedlings, stomatal conductance (g_s) tended to increase with warming in tamarack seedlings, while g_sdeclined with warming in spruce. In both species, CO₂ had …

Carbon And Nitrogen Dynamics In Plants Grown At Low Co2 Conditions Of The Past, Andre Galvao Duarte

Electronic Thesis and Dissertation Repository

For most of the recent evolutionary history of plants, atmospheric CO₂ concentrations have been far below modern values. However, little is known about how plants grown in a low CO₂ environment balance their carbon demand for growth while meeting their requirement for nitrogen acquisition, the establishment of mycorrhizal fungal associations, and the production of defense compounds. Here, I investigated how low CO₂ affects Elymus canadensis and Picea mariana by comparing their growth at low and current CO₂ concentrations. I found that reduced N availability exacerbated low CO₂ effects on growth, and reduced stomatal index and …

Diverse Far-Red Light Utilization Strategies In Cyanobacteria And Algae, Benjamin Martin Wolf

Arts & Sciences Electronic Theses and Dissertations

In their natural environments, photosynthetic organisms are often exposed to widely varied light environments. Species adapted to shade light, often found growing in lower layers of photosynthetic biofilms, must survive on filtered light alone. Filtered light is highly enriched in far-red wavelengths, which are normally unavailable for photosynthetic energy production in most oxygenic phototrophs. To overcome light limitations in filtered light environments, some species of algae and cyanobacteria utilize specialized photosynthetic pigments and antenna systems to harvest these far-red wavelengths. By sampling the natural environment and using custom-built far-red light growth chambers, I have isolated several species of oxygenic phototrophs …

Photosynthesizing The Workplace: A Study In Healthy And Holistic Production Spaces, Kaeli Howard

Masters Theses

Throughout time nature has been a prescribed healer of stress on the human condition. Its vital integration into our daily lives has been proven by scientific evidence. The majority of Americans spend approximately 1/3 of their life working, whatever that job may entail. Therefore, it makes sense that the environments that we spend so much of our life in for work at extremely important to our physical and mental health, however, current workplace models are not acknowledging that. Redefining the workplace to integrate nature would start to change work life in this country and how work itself is viewed.

This …

Greening Rates And Photosynthetic Development Of Leaves In C3 And C4 Plants, Tayler J. Kriss

Master's Theses

To study chlorophyll development time and overall photosynthetic development in C3 and C4 leaves, seeds were germinated in complete darkness and achlorophyllous leaves were then allowed to develop in lighted conditions. Corn (Zea mays, C4), sorghum (Sorghum bicolor, C4), green bean (Phaseolus vulgaris, C3), broad bean (Vicia faba, C3), and wheat (Triticum aestivum, C3) were investigated for the first ten days of sunlight exposure. Chlorophyll concentration, chlorophyll fluorescence, and CO2 gas exchange measurements were conducted daily on the first leaf that emerged after the embryonic leaves of each plant. The first five days of the experiment, days zero to four …

Ecophysiological Responses Of Tropical Woody Species To Ambient And Elevated Temperatures, Kelsey R. Carter

Dissertations, Master's Theses and Master's Reports

Tropical forests cycle one third of Earth’s carbon, yet we are still unsure how tropical vegetation will respond to climate warming. Tropical biomes experience a smaller temperature margin compared to other systems, possibly making them less capable of thermal adjustments. In addition, thermal responses of vegetation have been identified as one of the areas of greatest uncertainty for global carbon models. This dissertation works to quantify tropical forest photosynthetic responses to temperature as well as assessing physiological thermal acclimation of four tropical species. In Chapter, 2 we conducted a meta-analysis to investigate global tropical photosynthetic responses to temperature. We presented …

Influence Of Loss Of Function Of The Fatty Acid Desaturase 7 Gene On Photosynthetic Activity And Foliar Redox Status, Janithri Savindya Wickramanayake

Graduate Theses and Dissertations

Fatty Acid Desaturase7 (FAD7) is a chloroplast-localized enzyme that converts 16 and 18 carbon dienoic fatty acids to trienoic fatty acids. The suppressor of prosystemin-mediated response2 (spr2) mutant in tomato (Solanum lycopersicum) and the fad7-1 mutant in Arabidopsis (Arabidopsis thaliana) result in the loss of function of FAD7, which alter the fatty acid profiles of chloroplast membranes and enhance resistance against aphids. This research contributes toward the long-term goal of identifying factors that determine aphid resistance in FAD7 mutants. Previous data suggested constitutive differences between spr2 and WT including increased expression of genes associated with photosynthesis and differences in redox …

Examination Of Carbon Assimilation And Nutrient Movement In Peach Trees (Prunus Persica (L.) Batsch) As A Result Of Variable Fall Climate, Brian Tyler Lawrence

All Theses

Deciduous fruit tree production worldwide may need to alter management strategies due to an increase of variable climate conditions. These include warmer than average temperatures during the year along with too much or little water during the growing season. Predicting best practices for orchard management will require a thorough understanding of how temperatures and soil moisture alter carbon acquisition and nutrient movement in trees, especially during the end of the growing season when tree reserves are accumulated for the following spring. Over two consecutive years, the effect of warmer fall temperatures, which delayed natural leaf senescence, and variable soil moisture …

Effects Of Elevated Temperature, Elevated Co2 And Photoperiod On Conifer Carbon Fluxes, Joseph R. Stinziano

Electronic Thesis and Dissertation Repository

Increasing temperatures due to rising atmospheric CO₂ concentrations will have direct effects on plant physiology, specifically photosynthetic carbon uptake. Changes in photosynthetic carbon uptake will alter feedbacks between vegetation and atmospheric CO₂, and changes in forest carbon dynamics will be important in determining whether vegetation amplifies or attenuates the effects of anthropogenic CO₂ emissions on climate. Coniferous trees, which are a large component of the boreal forest, are understudied in relation to thermal acclimation of photosynthesis and temperature effects on growth. In the present work, I assess the impact of rising temperatures on carbon fluxes in …

Effects Of In-Situ Leaf-Level Canopy Warming In A Northern Hardwood Forest, Kelsey Carter

Dissertations, Master's Theses and Master's Reports

Rising mean annual temperatures due to climate change have intensified the need to understand the effects of warming on plant physiological processes. Forest photosynthesis is the most important pathways of terrestrial carbon sequestration, yet continued warming could reduce this important carbon sink. Photosynthesis is highly sensitive to temperature and begins to decline after an optimum temperature (T_opt) is reached, leading to reduced carbon uptake. To date, logistical difficulties have limited our ability to test photosynthetic responses to sustained warming in mature forest canopies. In order to understand how elevated temperatures will affect forest ecosystems, we need to be …

Potential Impacts Of Climate Change On Photochemistry Of Zostera Marina L., Billur Celebi

OES Theses and Dissertations

Seagrasses account for approximately 10% of the ocean’s total carbon storage, although photosynthesis of seagrasses is carbon limited at today’s oceanic pH. Therefore, increasing atmospheric CO₂ concentration, which results in ocean acidification/carbonation, is predicted to have a positive impact on seagrass productivity. Previous studies have confirmed the positive influence of increasing CO₂ on photosynthesis and survival of the temperate eelgrass Zostera marina L., but the acclimation of photoprotective mechanisms in this context has not been characterized. This study aimed to quantify the long-term impacts of ocean acidification on photochemical control mechanisms that promote photosynthesis while simultaneously protecting …

Exploring The Possibility Of Photosynthetic Plasticity In Agave Sensu Lato, John Anthony Huber

Theses and Dissertations

Crassulacean acid metabolism (CAM) provides desert plants with distinct advantages over the C₃ and C₄ photosynthetic pathways in harsh climates where water is scarce. CAM is, however more metabolically costly than C₃ or C₄ photosynthesis, and some plants, such as Mesembryanthemum crystallinum, facultatively utilize CAM when water is abundant, and water conservation unnecessary. In such situations, these plants behave akin to a C₃ plant when photosynthesizing. CAM is divided into four phases, with each phase displaying unique metabolic processes. Certain changes, including changes in the timing of CO₂ fixation, stable carbon isotope ratios, …

Photosynthetic Acclimation To Warming And Elevated Co2 In Two Antarctic Vascular Plant Species, Vi Nt Bui

Electronic Thesis and Dissertation Repository

Climate change can affect the performance of the only two vascular plant species found in Antarctica, Deschampsia antarctica and Colobanthus quitensis. I investigated the response of these two species to warming and elevated CO₂ in terms of photosynthesis and leaf anatomy. While photosynthesis increased directly with rising temperature and CO₂, it showed no acclimation to changes in growth temperature, and a small degree of acclimation to growth under elevated CO₂. Likewise, leaf anatomy displayed little plasticity in response to changes in the growth environment, although D. antarctica’s stomatal groove structure was modified under …

Inferring Plastid Metabolic Pathways Within The Nonphotosynthetic Free-Living Green Algal Genus Polytomella, Sara Asmail

Electronic Thesis and Dissertation Repository

The advent of photosynthesis facilitated the evolution of aerobic life on Earth. However, species such as Prototheca wickerhamii and Plasmodium falciparum, among many others, have lost photosynthesis and opted for a free-living/parasitic lifestyle. Despite this loss, these species have retained the plastid for its metabolic pathways, without which they would die. Polytomella is a nonphotosynthetic free-living alga, closely related to the photosynthetic model organism Chlamydomonas reinhardtii, and has been shown to lack a plastid genome. I set out to determine Polytomella plastid metabolic pathways using bioinformatics to look for mRNA and DNA homologous sequences matching pathway enzymes in model organisms. …

The Effects Of Canopy Structure On Photosynthesis In Sphagnum Mosses, Eric Matthew Ward

Honors Theses

In open habitats, Sphagnum grows with a dense architecture to retain water; mosses adapted to survive in shade persist in looser canopies. It is unknown if there is a physiological advantage of such rough canopies in the shade, but they may have enhanced photosynthesis at low light angles as would occur during spring or fall. It was hypothesized that the rough canopies would have enhanced rates of photosynthesis at lower angles. To test this, a photosynthesis system interfaced with a custom chamber was used to generate light response curves. Preliminary results of the study found no difference in photosynthesis with …

Instantaneous Photosynthetic Response To Temperature Of Mature Forest Canopies And Experimentally Warmed Seedlings, Alida C. Mau

Dissertations, Master's Theses and Master's Reports - Open

Tropical trees have been shown to be more susceptible to warming compared to temperate species, and have shown growth and photosynthetic declines at elevated temperatures as little as 3oC above ambient. However, regional and global vegetation models lack the data needed to accurately represent physiological response to increased temperatures in tropical forests. We compared the instantaneous photosynthetic responses to elevated temperatures of four mature tropical rainforest tree species in Puerto Rico and the temperate broadleaf species sugar maple (Acer saccharum) in Michigan. Contrary to expectations, leaves in the upper canopy of both temperate and tropical forests had temperature …

Effects Of Excitation Pressure On Variegation And Global Gene Expression In Arabidopsis Thaliana, Rainer Bode

Electronic Thesis and Dissertation Repository

I assessed the effects of photosystem II excitation pressure on chloroplast biogenesis and leaf sectoring in the Arabidopsis thaliana variegated mutants im, spotty, var1, var2, chs5 and atd2. The plants were grown under varying degrees of excitation pressure induced by growth at increasing irradiance at different temperatures and the extent of variegation was quantified throughout the plant’s development. I found that the degree of variegation was positively correlated with excitation pressure, regardless of whether high light or low temperature was used to induce increased excitation pressure in all the mutants tested. This was irrespective of …

Use Of Capacitance Sensors For Development Of Conservative Irrigation Regimes, Ethan Daniel Hagen

Masters Theses

Several experiments were conducted to further develop capacitance sensor-based automated irrigation systems. The first experiment tested whether the photosynthetic response to decreasing volumetric water content (VWC) differed among four species tested. A sigmoidal curve best described the relationship for all species (r² [r-squared]>0.86). The VWC that maintained maximum photosynthesis at 90% was selected as a potential conservative irrigation set point and values were not different between species, nor were 100% container capacity values. This indicates that a single set point is adequate to initiate irrigation and that a common upper threshold for VWC can be used for this …

Physiological Effects Of Low Mowing Heights, Rolling, And Foot Traffic On Creeping Bentgrass Putting Greens, Joseph Ronald Young

Graduate Theses and Dissertations

Golf course superintendents are managing creeping bentgrass (Agrostis stolonifera L.) putting greens throughout the transition zone where temperatures can exceed optimum levels for consecutive days in the summer. The stress of creeping bentgrass associated with these supraoptimal temperatures has been well documented, but the management practices implemented on putting greens to increase green speeds may exacerbate these environmental stresses. To date, the physiological effects of these management practices in combination have not been evaluated for putting green turf. The objective of this dissertation project was to determine the effect of mowing heights, light-weight rolling, and foot traffic on performance and …

Chlorophyll Fluorescence And Thermal Stress In Archaias Angulatus (Class Foraminifera), Heidi M. Toomey

USF Tampa Graduate Theses and Dissertations

ABSTRACT

Benthic foraminifers that host algal symbionts are similar to corals in that they rely on their algal endosymbionts for their energy needs, calcify prolifically, and are sensitive to changes in environmental conditions. They are abundant in the benthos of coastal coral-reef areas and are found throughout the tropical and subtropical regions. Pulse Amplitude Modulated (PAM) chlorophyll fluorometry and chlorophyll a extraction techniques were used to quantify and compare the photosynthetic responses of the benthic foraminiferal, Archaias angulatus and their isolated endosymbionts, Chlamydomonas hedleyi, to short-term changes in temperature. Maximum quantum efficiency (F_v/F_m) and rapid …

Effect Of Water-Deficit Stress On Cotton During Reproductive Development, Dimitra Loka

Graduate Theses and Dissertations

Water deficit is a major abiotic factor limiting plant growth and crop productivity around the world. Cotton (Gossypium hirsutum L.) is considered to be relatively tolerant to drought and the effects of water stress on leaf physiology and metabolism have been extensively documented. However, information is lacking on the effect of water-deficit stress on the cotton flower. It was hypothesized that water-deficit stress would impair gas exchange functions which consequently would result in perturbation of carbohydrates of cotton reproductive units. To investigate this hypothesis growth room studies and field studies were conducted with the objectives being to document the physiological …