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Salinity In Propagation: Germination And Juvenile Acclimatization Of Wetland Halophytes Using Saline Irrigation, Morgan Tomlin

All Theses

The introduction of poor water quality into commercial nursery crop production is predicated on optimized methods that apply saline irrigation without compromising plant success and health. Halophytes have many mechanisms of salt tolerance; however, these are greatly tied to physiological and developmental maturity. Thus, evaluating salt tolerance of halophytic crops (Hibiscus moscheutos and Kosteletzkya virginica) during seed germination and juvenile phases of life may provide insight into the efficacy of integrating poor quality water in horticultural operations.

Four seed priming methods (hydropriming, proline priming, low concentration halopriming, and high concentration halopriming) were evaluated as pre-sowing techniques to ameliorate …

Identifying Optimum Germination Temperatures And Analysis Weights In Seed Testing For Stiff Goldenrod (Solidago Rigida) And River Bulrush (Bolboschoenus Fluviatilis), Rachel Geary

Electronic Theses and Dissertations

Federal and state seed laws require seed lots offered for sale to be tested and labeled with the appropriate information before they can be sold. Those tests are conducted in seed testing labs all around the world. In the United States, accredited seed testing labs follow the Association of Official Seed Analysts (AOSA) Rules for Testing Seeds to provide information for labeling. The rules lack instructions for weights and germination for many native species. Stiff goldenrod (Solidago rigida) and river bulrush (Bolboschoenus fluviatilis) are two species that lack any information required for testing. When seed testing labs use correct weights …

Buoyancy And Emergence In Elymus Species, Sandor Jakab

Williams Honors College, Honors Research Projects

It is important that native plants are able to successfully emerge in wetlands because they play an important role in maintaining diversity. The relationship between seed buoyancy and seedling emergence was analyzed for three wetland species of the same genus: Elymus canadensis (FACU), Elymus macgregorii (FACW), and Elymus virginicus (FACW). E. macgregorii seemed to be the most buoyant, and E. virginicus and E. canadensis demonstrated very similar, less buoyant levels. Based on the idea that more buoyant seeds will be deposited at a shallow depth, and less buoyant seeds will be deposited at a deeper soil level, a relationship between …

The Effects Of Drought And Shade On Bottomland Hardwood Regeneration, Charles Joseph Pell

LSU Doctoral Dissertations

Floodplain forests support a high diversity of tree species adapted to regenerate under fluctuating water and light availability. Regeneration regulates species composition, and shade and flood tolerance influence the likelihood of regeneration. Regeneration failure of shade-intolerant and flood-tolerant tree species commonly occurs in southcentral and southeastern floodplain forests of the United States, also known as bottomland hardwoods (BLHs). In many BLHs reduced flooding has resulted in a dryer floodplain. These changes in flooding are linked to recent shifts in species composition. The mechanisms controlling regeneration in BLHs and these composition shifts are poorly understood. In a controlled germination experiment, I …

Effects Of Scarification, Phytohormones, Stratification, Soil Types And Warming On The Germination And/Or Performance Of Three Tamaulipan Thornscrub Species, Paula Luera

Theses and Dissertations

The Tamaulipan thornforests of south Texas and northeast Mexico are an ecologically and economically important conservation hotspot. Thornforest restoration is limited by native tree and shrub seedling availability for planting. Seedling shortages arise from low seed availability and knowledge gaps regarding best practices for germinating and growing the 70+ thornforest species desired for restoration plantings. To fill key knowledge gaps, we investigated three ecologically important thornforest species with low or highly variable germination or seedling survival rates: Ebenopsis ebano, Cordia boissieri, and Zanthoxylum fagara. For each, we quantified the effects of different dosages of chemical seed treatments …

Propagation Of Two Utah Native Plants: Ceanothus Velutinus And Cercocarpus Montanus, Asmita Paudel

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Among various water conservative approaches, the use of native plants in landscape, such as Ceanothus velutinus (snowbrush ceanothus) and Cercocarpus montanus (alder-leaf mountain mahogany), is attractive. Efficient propagation methods are required to allow these native species to use in water-efficient landscaping. Sexual (seed) and asexual/vegetative (cuttings and micropropagation) propagation methods were evaluated.

Seeds of both C. velutinus and C. montanus were scarified and/or stratified and treated with gibberellic acid (GA₃) to break dormancy. The results showed hot water scarification and 2-3 months of stratification effectively broke the dormancy of C. velutinus seeds, and stratification for 2-3 months was …

Effects Of Germination Conditions Of Brown Rice In Relation To Flour Physicochemical Properties And Bread Qualities, Wipada Wunthunyarat

Graduate Theses and Dissertations

Gluten-free products from rice are gaining popularity among consumers because of its hypoallergenic characteristic. The absence of gluten results in inferior bread qualities such as hard texture, reduced volume and shorter shelf-life, which can be improved by the addition of external hydrolytic enzymes. Hydrolytic enzymes are activated during germination to stimulate plant growth, and hence these enzymes may function similarly to the external enzymes to improve gluten-free bread from brown rice. Therefore, the objective of this work was to investigate the activities of amylases and protease in germinated brown rice (GBR) from long-grain and short-grain rices under different germination conditions …

Warm Stratification Increases Germination Of Dendromecon Rigida And Ehrendorferia Chrysantha, Cesar Garcia

Electronic Theses, Projects, and Dissertations

We explored the seasonal factors that might play a role in triggering germination of Dendromecon rigidaand Ehrendorferia chrysantha.D. rigida and E. chrysantha have been found difficult to germinate using common greenhouse techniques, Keeley and Fotheringham successfully germinated both species after storing their seeds in the field over a year and treating them with smoke. Identifying the specific seasonally changing factors that stimulated germination could have implications for understanding germination requirements for these and other hard-to-germinate chaparral species.

Dendromecon rigidaand Ehrendorferia chrysanthaare part of the Papaveraceae family and both are found in the chaparral environment in …

Influence Of Shaded Conditions On Development Of Asteraceae Species Native To Kansas, Aline Rodrigues De Queiroz

Master's Theses

to the heterogeneity of habitats, all plants are exposed to at least some degree of shade during their lifetime. Reduced light intensity, drops in R:FR ratio, and limited blue light are cues for plants to perceive competition; the shade avoidance syndrome (SAS) is common for grassland species while shade tolerance (ST) is common for forest species when competition is perceived. SAS is characterized by elongation of stems and petioles, reduced branches, decreased leaf area, decreased shoot biomass, and increased number of ramets. ST is characterized by little elongation of stems and petioles, high chlorophyll content and high chlorophyll a/b ratio …

Plant Hormone Lab Module: Assessing Different Factors To Create The Most Effective Lab, Ahmed El-Kulak

Williams Honors College, Honors Research Projects

The research project focused on evaluating 3 different factors in creating a plant hormone lab module for Principles of Biology II lab. The main hormone of focus was brassinosteroid. In the model plant Arabidopsis thaliana, brassinosteroids promote cell elongation, cell division, root growth, and seed germination. Three goals were established to create the most efficient lab module possible. First, mutant (bes1,bri1) root length was assessed without addition of brassinolide (BL), a synthetic brassinosteroid. Then, ½ MS and ½ MS + 20% sucrose plates were compared to see which media produced the greatest difference in root growth between the mutant lines. …

Biological Soil Crusts In A Northeastern Pine Barren: Composition And Ecological Effects, Jessica Gilbert

Honors Theses

Biological soil crusts (BSCs), otherwise known as cryptogamic soil crusts, biocrusts, or cyanobacterial crusts, are soil aggregations hosting diverse biotic communities. They are composed of cyanobacteria and algae, and generally have a covering of moss and/or lichen. BSCs are typically found in arid to semi-arid regions throughout the world, and are integral soil stabilizers, moisture retainers, and nitrogen fixers in these communities. Along with these factors, BSCs are able to impact germination and establishment of plants, either as an accompanying influence, or direct result of those listed above. BSCs have yet to be formally described in the inland northeastern United …

Emerging Seed Enhancements To Reduce The Risk Of Sagebrush Post-Fire Seeding Failure, Ryan Scott Call

Theses and Dissertations

The sagebrush (Artemisia spp.) steppe is undergoing rapid ecological change. The degradation of sagebrush steppe rangelands has resulted in the listing of more than 350 animals and plants as species of conservation concern. In addition, there has been a decrease in recreational values, reduced forage production, degraded water resources, and an increase in fire frequency. In the sagebrush steppe, success rates for seeding sagebrush after wildfire are notoriously low. Not only are sagebrush seeds hard to sow due to their small size and associated flower parts, but seedlings are exposed to numerous stresses that lowers their survivability. To improve …

Ecosystem Recovery In Estuarine Wetlands Of The Columbia River Estuary, Sarah Ann Kidd

Dissertations and Theses

In the restoration of tidal wetland ecosystems, potential drivers of plant community development range from biotic controls (e.g. plant competition, seed dispersal) to abiotic controls (e.g. tidal flooding, salinity levels). How these controls influence the success of tidal wetland restoration are only partly understood, but have important implications for wetland habitat recovery. Specifically, the extent to which the existing native and non-native seed banks in tidally reconnected wetlands interact with these controls is not clear, yet the potential success of passive restoration methods depends upon this understanding.

For a 54-year chronosequence of eleven tidal wetland restoration sites in the Lower …

Sclerocactus Wetlandicus: Habitat Characterization, Seed Germination And Mycorrhizal Analysis, Kourtney T. Harding

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The Uinta Basin hookless cactus (Sclerocactus wetlandicus) is a threatened species native to Eastern Utah. The cactus is found in a landscape highly disturbed by non-renewable energy production. To understand the environmental conditions that support natural growth of this cactus, we asked what types of plants were present in the same areas as the cactus, and if the types of plants were different in environments that were disturbed. From our assessment, we determined that the types of plants present in disturbed areas were drastically different from those present in undisturbed locations. Areas previously used for energy production are …

Assessment Of Hard Red Spring Wheat Germplasm For Cold Temperature Germination Tolerance, Ulas Cinar

Electronic Theses and Dissertations

When planted early in the season, hard red spring wheat (HRSW; Triticum aestivum L.) can help for both vegetative growth and yield providing maximum agronomic productivity. The north-central Great Plains growing region is considered best for developing varieties that germinate in sub-optimal temperatures. Within limited regions, spring wheat planting typically occurs between mid-April and late May, when soil temperatures are 8 °C to 14 °C. Early season planting may result in freezing stress, while late season planting can result in heat and drought stress. Our study tested the germination rate of hard red spring wheat (HRSW) over a range of …

Diversity And Evolution Of Fruits In Cuscuta (Dodders; Convolvulaceae), Anna Ho

Theses and Dissertations (Comprehensive)

Cuscuta (dodder) is a genus of roughly 200 species of obligate stem parasites with sub-cosmopolitan distribution. The fruit, generally regarded as a capsule, has a thin pericarp containing one to four seeds and opening at the base (circumscissile dehiscence; DE), or remaining closed (indehiscent; IN). IN has evolved multiple times in Cuscuta from DE, and is most common in the North American clades of subgenus Grammica. In addition, some species produce fruits that open irregularly. Characteristics pertaining to the fruits of Cuscuta are important as their seeds contribute most to their distribution and prevalence across the globe, and their reduced …

Effect Of Germination And Parboiling On Milling, Physicochemical, And Textural Properties Of Medium- And Long-Grain Rough Rice, Ashley Han

Graduate Theses and Dissertations

Germinated brown rice is commonly consumed in Asia for its enhanced nutritional properties such as gamma-aminobutyric acid (GABA) and softer cooked texture. However germination decreases milling yield and alters some physicochemical properties, and most germinated rice studies evaluated medium-grain and aromatic rice cultivars. This study compared the effects of germination duration and parboiling on milling, physicochemical, and textural properties of a medium- (Jupiter) and a long-grain (Wells) rice cultivars. Rough rice was soaked in water at 25°C for 12 hr, and then incubated at 30-34°C for varying germination durations, or combined with parboiling at 120°C for 20 min prior to …

Identification Of Cyclophilin Gene Family In Soybean And Characterization Of Gmcyp1, Hemanta Raj Mainali

Electronic Thesis and Dissertation Repository

I identified members of the Cyclophilin (CYP) gene family in soybean (Glycine max) and characterized the GmCYP1, one of the members of soybean CYP. CYPs belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. PPIase catalyzes the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. After extensive data mining, I identified 62 different CYP genes in soybean (GmCYP1 to GmCYP62), of which 8 are multi-domain proteins and 54 are single domain proteins. At least 25% of the GmCYP genes are expressed in soybean. GmCYP1 …

The Role Of Oswrky71 And Its Interacting Proteins In Seed Germination And Early Growth Of Cereal Grains, Margaret Ja Shin

UNLV Theses, Dissertations, Professional Papers, and Capstones

During seed germination and early seedling growth, complex molecular and physiological events occur in rice (Oryza sativa) and other cereal grains. As the seed transitions to vegetative tissue, it responds to both favorable and unfavorable environmental conditions and is vulnerable to attack by predation and disease. Although seeds are relatively small and tender in size, extensive and sophisticated molecular networks enables the immobile seed to grow, survive and adapt in its environment. One of the networks I am interested in is in the crosstalk between the gibberellin (GA) and abscisic acid (ABA) signaling pathways. These pathways are interesting because they …

A Comparative Analysis Of The Relative Water Content Of The Pollen Of Early Diverging Angiosperms, Andrew Robert Moffatt

Chancellor’s Honors Program Projects

No abstract provided.

Evaluating Native Wheatgrasses For Restoration Of Sagebrush Steppes, Jayanti Ray Mukherjee

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Pseudoroegneria spicata and Elymus wawawaiensis are two native perennial bunchgrasses of North America's Intermountain West. Frequent drought, past overgrazing practices, subsequent weed invasions, and increased wildfire frequency have combined to severely degrade natural landscapes in the region, leading to a decline in the abundance of native vegetation. Being formerly widespread throughout the region, P. spicata is a favorite for restoration purposes in the Intermountain West. Elymus wawawaiensis, which occupies a more restricted distribution in the Intermountain West, is often used as a restoration surrogate for P. spicata. However, since most restoration sites are outside the native range of …

Is Oxidative Stress The Cause Of Death When Recalcitrant Spartina Alterniflora Seeds Are Dried?, James Hammond Chappell

LSU Doctoral Dissertations

Recalcitrant seeds, which die when desiccated, can be difficult to study because of their generally large size, high metabolism, and poor storage properties. However, recalcitrant seeds from the salt-marsh grass Spartina alterniflora are unique when compared to most other recalcitrant species because the seeds are dormant and small; Spartina pectinata and S. spartinae, which produce orthodox seeds, can be used as controls. Because of these somewhat rare characteristics, S. alterniflora is a good model system to study recalcitrance. In the present study, the following physiological parameters were examined: Cardinal temperatures for germination, a viability test to determine if seeds are …

Does Seed Size Affect The Rate Of Germination And Early Seedling Growth In Hairy Vetch?, Joy Ekpo

McCabe Thesis Collection

Size is an important characteristic of seed quality because larger seeds with larger volume contain more resources and are likely to exhibit greater vigor than smaller seeds (Ellis, 1992). In a range of plant species, seed size has been shown to affect germination rate, emergence rate, success of establishment, and growth (Black 1957; Bentley et al. 1980; Sanderson et al. 2002). Hairy vetch is an annual legume that is used for improvement of soil for pasture and to provide ground cover protecting against erosion (Duke 1981; Undersander 2002; Sattell et al 1988). Problems facing the establishment of hairy vetch are …

The Effectiveness Of Post-Fire Weed Management And The Germination Ecology Of Selected Invasive Weed Species Of Bold Park, Brett Neasham

Theses : Honours

Bold Park is a 437 hectare A class reserve with high conservation value. Bold park is located on the Swan Coastal Plain, approximately 11 km west of the Perth CBD. Bold park is one of the few remaining coastal bushland reserves on the Swan Coastal Plain. High levels of floristic and faunal diversity contribute to Bold Park's conservation value. Weed invasion in Bold Park is reducing floristic diversity and is modifying ecosystem processes. Principle in the modified ecosystem processes is fire. While fire is a natural disturbance event in the bushland of the south-west of Western Australia, altered fire regimes …

A Solid Phase Extraction System For The Isolation Of The Germination Cue In Smoky Water When Subjected To The Light Sensitive Lettuce Lactuca Sativa V. Grand Rapids, Troy David Adriansz

Theses : Honours

The search for the chemical or set of chemicals that act as a germination stimulant in Lactuca sativa v. Grand rapids (a variety of lettuce seed) was the underlying basis of this project. Through the development of a solid phase extraction (SPE) system different fractions of smoky water were tested for their effects on germination enhancement. The project targeted streamlining the amount of solvents as well as time used in past research on smoky water. Previously, several steps involving high perfo1mance liquid chromatography (HPLC), thin layer chromatography (TLC) and Gas Chromatography (GC) were employed. This project used SPE cartridges to …

The Method Of Radicle Emergence In Phytolacca Americana (Pokeweed), Staci A. Van Winkle

Honors Theses

Germination is a key element in the development of a plant. This process encompasses several stages, one of which is the elongation of the radicle tip. This process has been noted to occur by different methods such as rapid mitotic division or cell elongation. However, such a mechcmism has not been studied in Phytolacca americana (pokeweed). It was the intention of this study to observe at the cellular level the nature of the Phytolacca americana radicle during normal germination. This was done using a routine histological microtechnique which involves embedding in paraffin and sectioning with a microtome. The sections were …

The Effects Of Berry Extracts From Phytolacca Americana (Pokeweed) On Seed Germination, Kevin Blake Anderson

Honors Theses

Extracts from the berries of Phytolacca americana (Pokeweed) exhibit the ability to inhibit germination of a variety of seeds, including its own. This inhibition has been shown to be effective even in dilute samples of the extracts. However, it is not known whether the germination inhibition caused by these extracts is induced by physical or by chemical mechanisms. This research proposes that the germination inhibition is induced by chemical inhibitors present within the berries. In an attempt to uncover the chemical agent(s) responsible for the germination inhibition characteristics exhibited by berry extracts of Phytolacca americana (pokeberry extracts), several separation techniques …

Dungpat Microenvironmental Effects On Germination And Establishment Of Crested Wheatgrass, Ghulam Akbar

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Complementary greenhouse and field studies investigated the effects of ambient environmental conditions on cattle dungpat moisture, temperature, nutrient concentration, and crust formation dynamics, which in turn influence seed germination and seedling establishment in dungpats. 'Hycrest' crested wheatgrass [Agropyron desertorum (Fisch. ex Link) X A. cristatum (L.) Gaert.] was used as a representative revegetation species.

After collecting feces from Holstein steers that had been fed crested wheatgrass seeds, uniform dungpats were prepared and placed on two soil types (loam and coarse sand) in containers under three watering treatments (field capacity, 1/2 field capacity, and no water) in the greenhouse. Dungpat …

Effect Of Salinity (Nacl) On Germination, Growth, Ion Accumulation, And Protein Synthesis In Alfalfa (Medicago Sativa L.), Thamir S. Al-Niemi

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

To study the effect of NaCl stress on gene expression in alfalfa (Medicago sativa L.), greenhouse and laboratory experiments were conducted with 22 cultivars during germination and post-germination growth. The ability of alfalfa cultivars to germinate at the different NaCl concentrations was not related to their postgermination performance (salt tolerance) under those conditions.

Genetic effects were evident for Na and Cl ion uptake and accumulation in alfalfa shoots and roots. The strategies of alfalfa cultivars to cope with NaCl stress includes exclusion of Na from shoots and Cl from roots or from the whole plant depending on cultivar and …

The Role Of The Seed Coat In The After-Ripening Process Of American Pokeweed (Phytolacca Americana L.), Jason E. Cox

Honors Theses

As plants evolve into new forms and shapes, the reproductive structures are often the last to change because of the importance of successful reproductive organs to the survival of the plant's hereditary information. Because of this particular feature, one very common method of determining how advanced a species is evolutionarily is the study of its reproductive cycle (Gifford and Foster, 1989). A few advanced classes, most notably the general class groupings of the Gymnosperms and Angiosperms, have developed seeds as a part of their reproductive cycle. Normally, there are several stages through which the seed must go in order for …