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Plant Sciences

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2016

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Impacts Of Long-Term Precipitation Manipulation On Hydraulic Architecture, Xylem Function, And Canopy Status In A Piñon-Juniper Woodland, Patrick J. Hudson Dec 2016

Impacts Of Long-Term Precipitation Manipulation On Hydraulic Architecture, Xylem Function, And Canopy Status In A Piñon-Juniper Woodland, Patrick J. Hudson

Biology ETDs

The Southwestern US is predicted to become hotter and drier, as global climate change forces increasing temperatures and variability in timing and size of precipitation inputs. Drought stress has become more frequent in recent decades, and resulted in massive forest mortality in piñon-juniper woodlands. During recent severe droughts (2000-2003, 2009-2012), piñon pine (Pinus edulis Englem.) suffered disproportionately high mortality compared to co-occurring one-seed juniper (Juniperus monosperma [Engelm.] Sarg.). A large-scale precipitation manipulation experiment was established in a piñon-juniper woodland in central New Mexico to test hypotheses regarding tree survival and mortality with respect to altered water regimes. Our …

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Chemical And Non-Chemical Control Of Potato Pink Rot, Xuemei (Missi) Zhang Dec 2016

Chemical And Non-Chemical Control Of Potato Pink Rot, Xuemei (Missi) Zhang

Electronic Theses and Dissertations

Pink rot of potato (Solanum tuberosum) is a widespread soilborne disease that causes significant losses in the field and storage. It is caused by Phytophthora erythroseptica (Pethybr.), an oomycete pathogen that produces sexual spores that can survive in soil for years. The management of pink rot mainly relies on chemical control. However, the most effective chemical in pink rot control, mefenoxam, is losing its efficacy owing to the development of mefenoxam resistance in P. erythroseptica. To evaluate alternative fungicides (including chemical and biological fungicides) to mefenoxam in pink rot control, two greenhouse experiments and three field trials …

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Salvinia Molesta: An Assessment Of The Effects And Methods Of Eradication, Arti Lal Dec 2016

Salvinia Molesta: An Assessment Of The Effects And Methods Of Eradication, Arti Lal

Master's Projects and Capstones

Salvinia molesta is an invasive aquatic fern. It is now the second worse aquatic invader in the world. Since the 1930s, it has invaded most tropical and some temperate countries. S. molesta plants grow vegetatively and can increase in size rapidly. S. molesta can form thick mats of up to 1-meter-thick. There are a number of ways these thick mats negatively affect the environment: 1) reduce light to benthic organisms, 2) reduce oxygen in the water column for other organisms, 3) accumulate as organic matter at the bottom of the water column, 4) decrease nutrients for other organisms, and 5) …

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Antibacterial Activity And Chemical Characterization Of Resin From Sciadopitys Verticillata (Thunb.) Siebold And Zuccarini, David Ira Yates Dec 2016

Antibacterial Activity And Chemical Characterization Of Resin From Sciadopitys Verticillata (Thunb.) Siebold And Zuccarini, David Ira Yates

Doctoral Dissertations

Sciadopitys verticillata produces white viscous resin that is unique among the conifers. This research investigated effects of resin on bacteria from different ecological niches and the chemical composition of the resin. Each bacterial species was evaluated separately for response to winter- and summer-collected resins. Exposure to winter-collected resin reduced numbers of colonies of Bacillus cereus, Erwinia amylovora, Agrobacterium tumefaciens, and Escherichia coli and increased numbers of Xanthomonascampestris, Pseudomonas fluorescens, and Pseudomonas syringae. Summer-collected resin affectedpopulation growth of two bacterial species; population counts of E. amylovora decreased and those of P. fluorescens increased. Selected strains of …

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Utilizing Cover Crops To Improve Sustainability Of Conventional Weed Management Programs, Garret Brown Montgomery Dec 2016

Utilizing Cover Crops To Improve Sustainability Of Conventional Weed Management Programs, Garret Brown Montgomery

Doctoral Dissertations

Research was conducted from the fall of 2014 to the fall of 2016 to evaluate the optimal utilization of cover crops for weed control in no-till environments. Studies included a corn (Zea mays L.) termination timing study to evaluation different termination intervals of cereal rye (Cereal rye L.), hairy vetch (Vicia villosa Roth.), and a combination of cereal rye and hairy vetch cover crops on corn growth and development, an evaluation of a roller crimper for cover crop termination prior to corn study, an evaluation of a roller crimper for cover crop termination prior to soybeans [ …

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The Role Of African Leafy Vegetables In Food Security, Marcia M. Croft Dec 2016

The Role Of African Leafy Vegetables In Food Security, Marcia M. Croft

Open Access Dissertations

African leafy vegetables (ALVs) are a diverse set of crops grown across sub-Saharan Africa. They have been a staple of traditional diets and contain many critical micronutrients but their importance has been largely ignored by researchers and policymakers at the expense of imported crops. Availability, accessibility, and utilization of ALVs are limited by factors across the supply chain. This research investigated seed systems, drought tolerance, hydroponic production, market barriers, consumer preference, and the impact of gender as related to ALVs. We found that ALVs play an important role in food security and that critical differences exist between formal and informal …

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Annual Forage Cropping-Systems For Midwestern Ruminant Livestock Production, John Ernest Mcmillan Dec 2016

Annual Forage Cropping-Systems For Midwestern Ruminant Livestock Production, John Ernest Mcmillan

Open Access Dissertations

Annual forage cropping systems are a vital aspect of livestock forage production. One area where this production system can be enhanced is the integration of novel annual forages into conventional cropping systems. Two separate projects were conducted to investigate alternative forage options in annual forage production. In the first discussed research trial, two sets of crops were sown following soft red winter wheat (Triticum aestivum L.) grain harvest, at two nitrogen application rates 56 and 112 kg ha-1 . The first set of crops were C4 summer annuals seeded within two weeks of wheat grain harvest and included, brown …

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Managing Bacterial Wilt, Caused By Erwinia Tracheiphila, On Muskmelon With Early Control Of Striped Cucumber Beetle ( Acalymma Vittatum (F)), And Through Varietal Selection, Ahmad Shah Mohammadi Dec 2016

Managing Bacterial Wilt, Caused By Erwinia Tracheiphila, On Muskmelon With Early Control Of Striped Cucumber Beetle ( Acalymma Vittatum (F)), And Through Varietal Selection, Ahmad Shah Mohammadi

Open Access Dissertations

Muskmelon (Cucumis melo L), is an important vegetable crop in the United States. It is grown throughout the US, and Indiana ranked 4th in production after California, Arizona and Georgia with 12.4 thousand metric tons and market value of $7.6 million in 2015. Bacterial wilt of cucurbits, which is caused by Erwinia tracheiphila (E. F. Smith), and vectored by striped cucumber beetle (Acalymma vittatum (F)), is one of the most serious diseases of muskmelon that influences muskmelon quality and yield. Many cultivars of muskmelon are grown around the United States, especially in the Midwest. Muskmelon cultivars differ in …

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Improving And Elucidating Factors Regulating Black Walnut (Juglans Nigra L.) Clonal Propagation, Micah E. Stevens Dec 2016

Improving And Elucidating Factors Regulating Black Walnut (Juglans Nigra L.) Clonal Propagation, Micah E. Stevens

Open Access Dissertations

Black walnut (Juglans nigra L.) is a fine hardwood tree species native to the central hardwood region of the United States. High-quality black walnut timber is highly desirable. Traded in both regional and global markets, it has been used for veneer, and the manufacture of high-end products such as cabinets, furniture, and gunstocks. As a result of its high economic value, black walnut has been commercially cultivated for many years, and breeding programs have generated superior timber genotypes with improved marketable traits. Once elite genotypes were developed, it was quickly recognized that black walnut was recalcitrant to clonal propagation …

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Evaluation Of A Cool-Season Grass-White Clover Mixture For Low-Nitrogen Input Lawns, Gabriel Adam Macke Dec 2016

Evaluation Of A Cool-Season Grass-White Clover Mixture For Low-Nitrogen Input Lawns, Gabriel Adam Macke

Open Access Theses

Turfgrass lawns require supplemental nitrogen (N) to maintain green color and seasonal shoot density. Improper lawn fertilization with excess N or phosphorus has the potential to contaminate both surface and groundwater. Thus, to reduce the reliance on supplemental N fertilization, alternative strategies or novel turf systems like grass-legume mixtures need explored. White clover ( Trifolium repens L.) is a stoloniferous legume that biologically fixes N from the atmosphere and adds N into the soil via mineralization. The objective of this field study was to evaluate the persistence and feasibility of a cool-season grass-clover lawn mixture. A lawn grass mixture with …

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Residual Effects Of Nitrogen Fertilization On Soil Nitrogen Pools And Corn Growth, Meghan E. Moser Dec 2016

Residual Effects Of Nitrogen Fertilization On Soil Nitrogen Pools And Corn Growth, Meghan E. Moser

Open Access Theses

Given the dynamic nature of soil nitrogen (N), inorganic N fertilization to corn (Zea mays L.) has potential to alter N pool balance by creating an accumulation or depletion of soil N. Current corn N recommendations in the common corn-soybean rotation of Indiana strive to find the best N rate that maximizes producer profit. Increasing our understanding of soil N will inform producers if they should adjust fertilizer rates for corn to influence maintenance of organic N and Carbon. Our objective was to determine residual N effects from fertilized corn in a corn-soybean rotation by measuring (1) soil N …

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Cereal Rye Cover Crop Effects On Soil Physical And Chemical Properties In Southeastern Indiana, Joseph D. Rorick Dec 2016

Cereal Rye Cover Crop Effects On Soil Physical And Chemical Properties In Southeastern Indiana, Joseph D. Rorick

Open Access Theses

Cover crops are growing in popularity in the Midwest, although questions remain about how to include them most effectively in a corn-soybean ( Zea mays L. - Glycine max L.) rotation. This study was conducted to determine the effects of cereal rye ( Secale cereale L.) on soil bulk density and water retention, soil organic carbon, soil nitrogen, and water stable aggregate mean weight diameter after four years of cover crop growth and the effects on soil moisture over a five year period. The study was conducted at the Southeast Purdue Agricultural Center (SEPAC) on silt loam soils. A 14 …

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Forest Industry Byproducts Improve Soil Quality And Increase Pepper Growth In Three Soils Infested With Phythophthora Blight, Xiaojun Zhao Dec 2016

Forest Industry Byproducts Improve Soil Quality And Increase Pepper Growth In Three Soils Infested With Phythophthora Blight, Xiaojun Zhao

Open Access Theses

Phytophthora blight is a serious threat to the Midwest vegetable industry, because the oomycete pathogen responsible for this disease, Phytophthora capsici, has a wide host range, can spread quickly in fields, and produces resilient oospores that can survive in soil for years. Phytophthora capsici has become resistant to commonly used fungicides and resistant crop varieties are rare. Amending soil with complex organic substrates has potential to improve soil quality and suppress soil-borne pathogens including P. capsici. Indiana has a significant forest industry with many residual products that could be used as locally available amendments to meet this goal. …

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Physiological Aspects Of Relative Changes In Nitrogen And Plant Density Stress Tolerances Over A 38-Year Period Of Us Maize Hybrid Introductions, Keru Chen Dec 2016

Physiological Aspects Of Relative Changes In Nitrogen And Plant Density Stress Tolerances Over A 38-Year Period Of Us Maize Hybrid Introductions, Keru Chen

Open Access Dissertations

Incremental gains in grain yield of maize hybrids over the decades are the consequence of genotype, environment and management interactions. Historically, genetic improvements in newer hybrids have included longer active grain filling periods (achieved by advancing silking and extending functional stay green in maize leaves); stronger source and sink during grain filling; enhanced tolerance to higher density; and canopy architecture changes. Newer hybrids were known to accumulate more dry matter and nitrogen in the post-silking period, but achieving a more comprehensive knowledge of pre-silking and post-silking dynamics required further understanding of dry matter and nitrogen partitioning in individual organs, as …

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The Biology And Management Of Palmer Amaranth (Amaranthus Palmeri S. Wats) In Indiana, Douglas J. Spaunhorst Dec 2016

The Biology And Management Of Palmer Amaranth (Amaranthus Palmeri S. Wats) In Indiana, Douglas J. Spaunhorst

Open Access Dissertations

Palmer amaranth is a pernicious summer annual weed that has evolved resistance to six herbicide sites of action in the U.S. and threatens agriculture production. In 2011, Palmer amaranth was identified in flood plains in two southern Indiana counties (Posey and Vanderburgh). Determining if Palmer amaranth can survive and reproduce in northern Indiana may provide insight if this weed will be problematic for northern row crop producers. The objectives of our research were to identify fields containing Palmer amaranth and determine the distribution of herbicide resistance traits in Indiana; determine the influence of tillage frequency and tillage intervals on Palmer …

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Dynamic Regulation Of Dna Demethylation And Rna-Directed Dna Methylation In Arabidopsis, Kai Tang Dec 2016

Dynamic Regulation Of Dna Demethylation And Rna-Directed Dna Methylation In Arabidopsis, Kai Tang

Open Access Dissertations

DNA methylation is an important epigenetic mark present in many eukaryotes, and is involved in many crucial biological processes, such as gene imprinting, regulation of gene expression, and genome stability. Proper genomic DNA methylation patterns are achieved through the concerted action of DNA methylation and demethylation pathways. In the model plant species Arabidopsis thaliana, ROS1 (REPRESSOR OF SILENCING 1) is one of the DNA demethylases and the key component in the demethylation pathway. Dysfunction of ROS1 leads to increase in DNA methylation level at thousands of genomic loci. However, the features of ROS1 targets are not well understood. In the …

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Genetic Study Of Carotenoids In Maize Grain (Zea Mays L.), Oscar Rafael Espejel Venado Dec 2016

Genetic Study Of Carotenoids In Maize Grain (Zea Mays L.), Oscar Rafael Espejel Venado

Open Access Theses

Pro-Vitamin A (proVA) carotenoids, which are converted into retinol (Vitamin A) in the human body, have been the subject of human nutrition studies and are a target for biofortification of staple crops. Historically, β-carotene has been the principal target for enhancing levels of proVA, yet there is recent interest in enhancing the proVA carotenoid β-cryptoxanthin. Studies have shown that β-cryptoxanthin has excellent bioavailability, and its use in maize may be nearly as effective as β-carotene in providing retinol. The primary aim of this study was to gain a better understanding of the genetic control of levels of β-cryptoxanthin, conversion of …

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The Effects Of Shade On Growth, Development And Yield Of A Primocane Fruiting Blackberry, ‘Prime-Ark 45’ To Extend The Market Season, Olivia C. Caillouet Dec 2016

The Effects Of Shade On Growth, Development And Yield Of A Primocane Fruiting Blackberry, ‘Prime-Ark 45’ To Extend The Market Season, Olivia C. Caillouet

Horticulture Undergraduate Honors Theses

This thesis examines the effects of shade on ‘Prime-Ark 45’ blackberries (Rubus spp.) in greenhouse (GH) and field (FD) experiments aimed at improving fruit production in the southern United States Ozark Plateau region. Primocane blackberry production in the southern United States is limited in acreage of production as well as low yields by high temperatures during the bloom and early fruiting period, resulting in poor fruit set and poor fruit quality. Shade may have the potential to delay bloom and flowering to a more favorable season or by reducing temperatures that cause a poor fruit set and quality. Both …

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An Investigation Of Ssglux, A Glucosidase Co-Expressed With Cslf6 In Oat (Avena Sativa) And Barley (Hordeum Vulgare), Michael Christopher Gines Dec 2016

An Investigation Of Ssglux, A Glucosidase Co-Expressed With Cslf6 In Oat (Avena Sativa) And Barley (Hordeum Vulgare), Michael Christopher Gines

Theses and Dissertations

Mixed Linkage Glucan (MLG, or (1,3;1,4)-ß-D glucan) is a component of cell walls for major cereal crops and is significant to food and beverage industries. To better understand genetic factors affecting MLG content in oats, this study investigates the presence of glucosidases likely to participate in MLG production. A glucosidase showing co-expression with CslF6—the primary gene responsible for MLG synthesis—could indicate a hand in MLG production by association. Reference genes for expression analysis as well as glucosidase candidates were first selected using in silico methods. In both cases, barley was used as model species because it has abundant public bioinformatic …

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Reproductive Ecology Of Greater Sage-Grouse In Strawberry Valley, Utah, Jared Jeffrey Baxter Dec 2016

Reproductive Ecology Of Greater Sage-Grouse In Strawberry Valley, Utah, Jared Jeffrey Baxter

Theses and Dissertations

Greater sage-grouse (Centrocercus urophasianus; hereafter, sage-grouse) are a species of conservation concern in the rangelands of western North America due to their dramatic decline over the last half century. Effective conservation and management of sensitive species requires an understanding of how species respond to management actions. We examined two aspects of the reproductive phases of sage-grouse: nest predation, and habitat selection by female sage-grouse with chicks. In Chapter 1, we developed resource selection functions to assess the influence of mechanical treatments of mountain big sagebrush (Artemisia tridentata vaseyana) on habitat selection by greater sage-grouse with chicks. …

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Ancient Maya Agricultural Resources In The Rio Amarillo Valley Near Copán, Honduras, Bryce Matthew Brown Dec 2016

Ancient Maya Agricultural Resources In The Rio Amarillo Valley Near Copán, Honduras, Bryce Matthew Brown

Theses and Dissertations

The purpose of this study was to use soil physical and chemical analyses to better understand the ancient agricultural landscape around the ancient Maya cities of Rio Amarillo and Piedras Negras, two tributary sites to Copan, Honduras. Our primary objective was to determine whether a mass erosion event around 800 A.D. occurred which could have caused crop failure and famine or if stable soil conditions persisted during the collapse of these city-states. Stable carbon isotope analysis of the humin fraction of the soils showed that much of this valley was used anciently for agriculture, including hillslopes and hilltops; however, there …

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Improved Fungicidal Control Of Large Patch Through Optimal Use Of Surfactants And Spray Rate Volume, Jesse J. Benelli Dec 2016

Improved Fungicidal Control Of Large Patch Through Optimal Use Of Surfactants And Spray Rate Volume, Jesse J. Benelli

Doctoral Dissertations

Large patch (Rhizoctonia solani AG 2-2LP) epidemics cause significant damage to Japanese lawngrass (JLG; Zoysia japonica) in the transition zone. Large patch primarily affects the stems and sheaths of JLG and is difficult to control using traditional fungicide sprays. Field and growth chamber experiments were conducted during 2015-2016 in TN and GA to evaluate methods to enhance fungicidal control of large patch in JLG landscapes. The 1st experiment evaluated the most critical application target site that resulted in the greatest amount large patch control. In this experiment, four fungicides (azoxystrobin, flutolanil, tebuconazole, and chlorothalonil) were dispensed as …

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The Effects Of Rootstock, Scion, Grafting Method And Plant Growth Regulators On Flexural Strength And Hydraulic Resistance Of Apple, Stuart W. Adams Dec 2016

The Effects Of Rootstock, Scion, Grafting Method And Plant Growth Regulators On Flexural Strength And Hydraulic Resistance Of Apple, Stuart W. Adams

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The apple rootstock ‘Geneva® 41’ (‘G.41’), is desirable for its resistance to fire blight, Erwinia amylovora, while producing high yields. However, ‘G.41’ and other Geneva® selections tend to form weak graft unions in the nursery that are susceptible to breaking in the wind, at harvest, or during shipment. In order to understand and remedy this graft union weakness, six scions (‘Fuji’, ‘Gala’, ‘Honeycrisp’, ‘McIntosh’, ‘Pink Lady’, and ‘Scilate’) by seven rootstocks (‘G.41’, ‘G.935’, ‘G.214’, ‘G.11’, ‘M.9-NIC 29’, ‘B.9’ and ‘EMLA 26’), by two grafting methods (chip bud and saddle graft) in a factorial design were tested for graft …

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Switchgrass Extractives Have Potential As A Value-Added Antimicrobial Against Plant Pathogens And Foodborne Pathogens, Alexander Ian Bruce Dec 2016

Switchgrass Extractives Have Potential As A Value-Added Antimicrobial Against Plant Pathogens And Foodborne Pathogens, Alexander Ian Bruce

Masters Theses

Panicum virgatum (switchgrass), a perennial grass native to North America, is a leading biomass feedstock candidate for the manufacture of cellulosic ethanol. Switchgrass is considered a viable option for biofuel production due to its cheap production cost and ability to grow on marginal land. Biofuel derived from switchgrass has been shown to be very energy efficient, producing 540% more renewable energy versus nonrenewable energy expended. Switchgrass-derived biofuel is also estimated to have greenhouse gas emissions that are 94% lower than emissions from gasoline (Schmer et al 2008). Biofuels are created through biochemical processes that utilize various enzymes and microorganisms for …

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Ultrastructural Changes During Pollen Wall Development And Germination In Arabidopsis Thalaiana, Katrina Olsen Dec 2016

Ultrastructural Changes During Pollen Wall Development And Germination In Arabidopsis Thalaiana, Katrina Olsen

Theses and Dissertations

The Arabidopsis thaliana meiotic mutant 6491 has been identified as displaying temperature sensitive male reduced- fertility. It has been determined that callose wall formation is defective, both in temporal and structural areas. There is irregular rippling in the plasma membrane and aberrant formation of the exine portion of the pollen wall. A developmental study using brightfield, epifluorescence, and transmission electron microscopy of the early stages of wall formation in 6491 has been completed, along with a similar study of Arabidopsis thaliana (L.) heynh, ecotype Wassilewskija (WS). Due to the temperature-sensitive nature of the mutant line, a further study of both …

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Characterization Of Fusarium Species And Alternaria Alternata, And Their Effects On Switchgrass Health And Chemical Components, Sara Beth Collins Dec 2016

Characterization Of Fusarium Species And Alternaria Alternata, And Their Effects On Switchgrass Health And Chemical Components, Sara Beth Collins

Masters Theses

Panicum virgatum L., readily referred to as Switchgrass, is a perennial warm-season bunch grass, used as an alternative energy source for biofuel production. There is insufficient research on switchgrass pathogens, and is expected that an increase in disease pressure will result as more land is reserved for this perennial crop. The purpose of this research was to identify and characterize pathogenic Alternaria alternata and Fusarium species on Switchgrass (Panicum virgatum L.), and to evaluate their impact on stand establishment and plant health. Molecular identifications of fungal isolates obtained from infected commercial switchgrass seed yielded eight species (A. alternata, …

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Rapid Molecular Detection And Population Genetics Of Pityophthorus Juglandis, A Vector Of Thousand Cankers Disease In Juglans Spp., Emel Oren Dec 2016

Rapid Molecular Detection And Population Genetics Of Pityophthorus Juglandis, A Vector Of Thousand Cankers Disease In Juglans Spp., Emel Oren

Masters Theses

Thousand Cankers Disease (TCD) is a disease complex involving the fungal pathogen Geosmithia morbida, an insect vector Pityophthorus juglandis, and the hosts, Juglans spp. and Pterocarya spp. Signs and symptoms of TCD include crown thinning due to branch dieback, yellowing and wilting of the leaves, appearance of epicormic shoots, numerous entrance/exit holes, gallery formation by P. juglandis, and the development of small, dark brown cankers underneath the bark. TCD originally described from western U.S., has now expanded to eastern U.S. and northwestern Italy. The disease complex is often difficult to diagnose due to the absence of symptoms …

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Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal Dec 2016

Identification And Characterization Of Genes Involved In Metabolism Of N5 Monoene Precursors To N5 Anacardic Acids In The Trichomes Of Pelargonium X Hortorum., Richa A. Singhal

Electronic Theses and Dissertations

Unusual monoenoic fatty acids (UMFA’s) and specialized metabolites called anacardic acids (AnAc) are produced in glandular trichomes of Pelargonium ´ hortorum (geranium). The UMFA’s, 16:1∆11 and 18:1∆13 are precursors for the synthesis of unsaturated AnAc 22:1n5and 24:1n5 that contribute to pest resistance in geraniums. UMFAs and their derived AnAc metabolites not only provide a useful biological marker that differentiates the biosynthetic pathway for unusual mononenes from the common fatty acids (i.e. stearic, palmitic, oleic, linoleic and linolenic) but also have industrial, medical and agricultural applications. Fatty acid biosynthesis enzymes like acyl carrier proteins (ACPs); thioesterases (TEs) and β-ketoacyl-ACP …

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Comparison Of Mesophyll Protoplast Isolation And Transformation Between Panicum Virgatum And Panicum Hallii, Andrea Grace Collins Dec 2016

Comparison Of Mesophyll Protoplast Isolation And Transformation Between Panicum Virgatum And Panicum Hallii, Andrea Grace Collins

Masters Theses

Protoplasts are appropriate targets for genome editing, DNA functional screens and transgenesis. This project focuses on the comparison of inexpensive mesophyll protoplast isolation via the use of food-grade enzymes and transformation between diploid Panicum hallii Vasey (PAH) and polyploid cellulosic feedstock Panicum virgatum L (switchgrass), a relative of PAH. PAH has great potential as a C4 model species for crop and bioenergy research. Here an inexpensive switchgrass and PAH mesophyll protoplast isolation and transformation system was developed; the first protoplast system for PAH. Using low-cost commercial food-grade enzymes, a cost reduction of ~1000-fold was achieved compared to traditional protoplast isolating …

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Calculating Evapotranspiration Of Arundo Donax Along The Rio Grande, Jose R. Escamilla Jr. Dec 2016

Calculating Evapotranspiration Of Arundo Donax Along The Rio Grande, Jose R. Escamilla Jr.

Theses and Dissertations

Invasive species have been recorded to cause large economic and ecological impacts on various ecosystems and their services. Locally, giant reed (Arundo donax L.), a woody grass native to the Mediterranean, adversely impacts riparian ecosystems in southwestern United States by aggressively displacing their native flora and fauna. Giant reed also has become a cause of concern for national water security, especially in water-limited areas of the arid southwestern United States. The main objective of this study is to provide the first, landscape-level estimates of water use by giant reed in the United States. Evapotranspiration was monitored using the eddy …

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