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Mir319a Targeting Of Tcp4 Is Critical For Petal Growth And Development In Arabidopsis, Anwesha Nag, Stacey King, Thomas Jack Dec 2009

Mir319a Targeting Of Tcp4 Is Critical For Petal Growth And Development In Arabidopsis, Anwesha Nag, Stacey King, Thomas Jack

Dartmouth Scholarship

In a genetic screen in a drnl-2 background, we isolated a loss-of-function allele in miR319a (miR319a129). Previously, miR319a has been postulated to play a role in leaf development based on the dramatic curled-leaf phenotype of plants that ectopically express miR319a (jaw-D). miR319a129 mutants exhibit defects in petal and stamen development; petals are narrow and short, and stamens exhibit defects in anther development. The miR319a129 loss-of-function allele contains a single-base change in the middle of the encoded miRNA, which reduces the ability of miR319a to recognize targets. Analysis of the expression patterns of the …


Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Sichul Lee, Jeff C. Chiecko, Sun A. Kim, Elsbeth L. Walker, Youngsook Lee, Mary Lou Guerinot, Gyhheung An Jun 2009

Disruption Of Osysl15 Leads To Iron Inefficiency In Rice Plants, Sichul Lee, Jeff C. Chiecko, Sun A. Kim, Elsbeth L. Walker, Youngsook Lee, Mary Lou Guerinot, Gyhheung An

Dartmouth Scholarship

Uptake and translocation of metal nutrients are essential processes for plant growth. Graminaceous species release phytosiderophores that bind to Fe3+; these complexes are then transported across the plasma membrane. We have characterized OsYSL15, one of the rice (Oryza sativa) YS1-like (YSL) genes that are strongly induced by iron (Fe) deficiency. The OsYSL15 promoter fusion to β-glucuronidase showed that it was expressed in all root tissues when Fe was limited. In low-Fe leaves, the promoter became active in all tissues except epidermal cells. This activity was also detected in flowers and seeds. The OsYSL15:green …


Identification Of Pmt, Tr1, And H6h Gene Polymorphism And Tropane Alkaloid Chemotypes In Hyoscyamus Niger L. (Black Henbane), Lawrence Kramer Jan 2009

Identification Of Pmt, Tr1, And H6h Gene Polymorphism And Tropane Alkaloid Chemotypes In Hyoscyamus Niger L. (Black Henbane), Lawrence Kramer

Masters Theses 1911 - February 2014

No abstract provided.


Microsatellites Reveal Genetic Diversity In Rotylenchulus Reniformis Populations, R. S. Arias, Salliana R. Stetina, Jennifer L. Tonos, Jodi A. Scheffler, Brian E. Scheffler Jan 2009

Microsatellites Reveal Genetic Diversity In Rotylenchulus Reniformis Populations, R. S. Arias, Salliana R. Stetina, Jennifer L. Tonos, Jodi A. Scheffler, Brian E. Scheffler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Rotylenchulus reniformis is the predominant parasitic nematode of cotton in the Mid South area of the United States. Although variable levels of infection and morphological differences have been reported for this nematode, genetic variability has been more elusive. We developed microsatellite-enriched libraries for R. reniformis, produced 1152 clones, assembled 694 corstigs, detected 783 simple sequence repeats (SSR) and designed 192 SSR-markers. The markers were tested on six R. reniformis cultures from four states, Texas, Louisiana, Mississippi and Georgia, in the USA. Based oil performance we selected 156 SSR markers for R. reniformis from which 88 were polymorphic across the …