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Spotted Cotton Oligonucleotide Microarrays For Gene Expression Analysis, Joshua A. Udall, Lex E. Flagel, Foo Cheung, Andrew W. Woodard, Ran Hovav, Ryan Adam Rapp, Jordan M. Swanson, Jinsuk J. Lee, Alan R. Gingle, Dan Nettleton, Christopher D. Town, Z. Jeffrey Chen, Jonathan F. Wendel
Spotted Cotton Oligonucleotide Microarrays For Gene Expression Analysis, Joshua A. Udall, Lex E. Flagel, Foo Cheung, Andrew W. Woodard, Ran Hovav, Ryan Adam Rapp, Jordan M. Swanson, Jinsuk J. Lee, Alan R. Gingle, Dan Nettleton, Christopher D. Town, Z. Jeffrey Chen, Jonathan F. Wendel
Dan Nettleton
Microarrays offer a powerful tool for diverse applications plant biology and crop improvement. Recently, two comprehensive assemblies of cotton ESTs were constructed based on three Gossypium species. Using these assemblies as templates, we describe the design and creation and of a publicly available oligonucleotide array for cotton, useful for all four of the cultivated species. Synthetic oligonucleotide probes were generated from exemplar sequences of a global assembly of 211,397 cotton ESTs derived from >50 different cDNA libraries representing many different tissue types and tissue treatments. A total of 22,787 oligonucleotide probes are included on the arrays, optimized to target the …
Duplicate Gene Expression In Allopolyploid Gossypium Reveals Two Temporally Distinct Phases Of Expression Evolution, Lex E. Flagel, Joshua A. Udall, Dan Nettleton, Jonathan F. Wendel
Duplicate Gene Expression In Allopolyploid Gossypium Reveals Two Temporally Distinct Phases Of Expression Evolution, Lex E. Flagel, Joshua A. Udall, Dan Nettleton, Jonathan F. Wendel
Dan Nettleton
Polyploidy has played a prominent role in shaping the genomic architecture of the angiosperms. Through allopolyploidization, several modern Gossypium (cotton) species contain two divergent, although largely redundant genomes. Owing to this redundancy, these genomes can play host to an array of evolutionary processes that act on duplicate genes. We compared homoeolog (genes duplicated by polyploidy) contributions to the transcriptome of a natural allopolyploid and a synthetic interspecific F1 hybrid, both derived from a merger between diploid species from the Gossypium A-genome and D-genome groups. Relative levels of A- and D-genome contributions to the petal transcriptome were determined for 1,383 gene …
Working With Plant Fibers, Laura K. Thompson Dr
Working With Plant Fibers, Laura K. Thompson Dr
Laura K Thompson Dr
This laboratory is one in a series that I use in Biology 401: Applied Plant Science. This course is designed for students who have had at least one introductory Biology course, either for a major or a non-major. The goal in the course is to give the student an appreciation for the importance of plants to human society. Each laboratory session is designed to give the student an appreciation for how plants contribute to society and an experience in original processing of plants for their use. In this laboratory session the students get a feel for the processes that are …
A Comparison Of Three Methods For Determining Root-Knot Nematode Infection Of Cotton Roots, Robert G. Mcbride, Robert Mikkelsen
A Comparison Of Three Methods For Determining Root-Knot Nematode Infection Of Cotton Roots, Robert G. Mcbride, Robert Mikkelsen
Robert Mikkelsen
Three methods for determining the extent of infection of cotton roots (Gossypium hirsutum L.) by root-knot nematode, Meloidogyne incognita (Kofoid and White) Chitwood, were compared. Root-knot nematode eggs were added to pots containing either sterilized soil or soil amended with rye, and cotton seedlings were planted. Cotton root systems were then assessed for infection/damage after 14 and 28 days of exposure. At the 14-day sampling, the roots were stained, using the lactophenol acid fuchsin method and the juvenile nematodes counted. At the 28-day samplings, the cotton roots were given a visual rating for nematode-induced root galling (0-100), and the galls …