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Transmittance And Reflectance Measurements Of Corn Leaves From Plants With Different Nitrogen And Water Supply, James S. Schepers, T. M. Blackmer, Wallace Wilhelm, M. Resende Nov 1996

Transmittance And Reflectance Measurements Of Corn Leaves From Plants With Different Nitrogen And Water Supply, James S. Schepers, T. M. Blackmer, Wallace Wilhelm, M. Resende

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Nitrogen is essential for crop production, but also contributes to eutrophication of surface water and degradation of drinking water quality. Modern corn production requires relatively large quantities of N, which are generally supplied by fertilizers. Over-application of N fertilizers and animal wastes frequently results in nitrate leaching. Synchronizing N availability with crop N need offers the potential to protect the environment without sacrificing production. Tools are needed to rapidly and easily monitor crop N status to make timely decisions regarding fertilizer application. Analytical and optical techniques were evaluated with greenhouse grown corn at silking to evaluate several methods to monitor …


Monoclonal Antibodies For Detection Of The H7 Antigen Of Escherichia Coli, Yongsheng He, James E. Keen, Ralph B. Westerman, E. Travis Littledike, Jimmy Kwang Sep 1996

Monoclonal Antibodies For Detection Of The H7 Antigen Of Escherichia Coli, Yongsheng He, James E. Keen, Ralph B. Westerman, E. Travis Littledike, Jimmy Kwang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two murine monoclonal antibodies (MAbs) (2B7 and 46E9-9) reactive with the H7 flagellar antigen of Escherichia coli were produced and characterized. A total of 217 E. coli strains (48 O157:H7, 4 O157:NM, 23 O157:non-H7, 22 H7:non-O157, and 120 non-O157:non-H7), 17 Salmonella serovars, and 29 other gramnegative bacteria were used to evaluate the reactivities of the two MAbs by indirect enzyme-linked immunosorbent assay (ELISA). Both MAbs reacted strongly with all E. coli strains possessing the H7 antigen and with H23- and H24-positive E. coli strains. Indirect ELISA MAb specificity was confirmed by inhibition ELISA and by Western blotting (immunoblotting), using partially …


Spikelet And Floret Naming Scheme For Grasses With A Spike Inflorescence, Wallace Wilhelm, Gregory S. Mcmaster Jul 1996

Spikelet And Floret Naming Scheme For Grasses With A Spike Inflorescence, Wallace Wilhelm, Gregory S. Mcmaster

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Effective schemes to name uniquely and define parts of grass plants allow researchers to communicate results of their experiments accurately and precisely. Naming schemes for vegetative parts of grass plants have been developed; however, no system exists to name uniquely parts of the inflorescence. In this report, we present a method to extend existing systems to name parts of grass spikes. Spikelet positions are denoted by the letter S and numbered acropetally along the rachis. For example, the third spikelet position from the base of the rachis is named S3. Likewise, floret positions along the rachilla are named F (or …


The Structural Gene Encoding Human Enterotoxigenic Escherichia Coli Pcfo20 Is Homologous To That For Porcine 987p, Gloria Inés Viboud, Gunhild Jonson, Evelyn A. Dean-Nystrom, Ann-Mari Svennerholm Apr 1996

The Structural Gene Encoding Human Enterotoxigenic Escherichia Coli Pcfo20 Is Homologous To That For Porcine 987p, Gloria Inés Viboud, Gunhild Jonson, Evelyn A. Dean-Nystrom, Ann-Mari Svennerholm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Putative colonization factor PCFO20 was recently identified in an enterotoxigenic Escherichia coli (ETEC) strain of serogroup O20 isolated from a child with diarrhea in Argentina. The gene encoding the structural subunit of PCFO20 fimbriae, fotA, was cloned from strain ARG-2 in the expression phage vector lambda ZAP Express. One positive clone, pGV29, that carried a 3.3-kb fragment was identified on the basis of fimbrillin production by using a monospecific rabbit anti-PCFO20 serum. Nucleotide sequencing of a 1.3-kb Sau3A-ClaI fragment of the subclone pGV292 containing the region coding for PCFO20 fimbrillin revealed two open reading frames of …


Planting Date And Residue Rate Effects On Growth, Partitioning, And Yield Of Corn, Stephan Swanson, Wallace Wilhelm Mar 1996

Planting Date And Residue Rate Effects On Growth, Partitioning, And Yield Of Corn, Stephan Swanson, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Corn (Zea mays L.) growth has been shown to be affected by planting date and amount of residue on the soil surface. The objective of this study was to determine if the adverse effects of cool soil temperatures on early seedling growth often associated with surface residues can be overcome by planting at later dates. Corn was planted at several planting dates (late April through early June) into soil that had residue applied at rates of 0, 33, 66, and 100% of that produced by the previous crop. In general, spring-applied residue rates had little effect on measured plant …


Tallgrass Prairie Vegetation Response To Spring Burning Dates, Fertilizer, And Atrazine, Robert B. Mitchell, Robert A. Masters, Steven S. Waller, Kenneth Moore, Linda J. Young Mar 1996

Tallgrass Prairie Vegetation Response To Spring Burning Dates, Fertilizer, And Atrazine, Robert B. Mitchell, Robert A. Masters, Steven S. Waller, Kenneth Moore, Linda J. Young

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Tallgrass prairies provide an important source of hay and summer forage in eastern Nebraska. A study was conducted in 1989 and 1990 on 2 late seral tallgrass prairies near Lincoln and Virginia, Nebraska to determine if production of selected components of tallgrass prairie communities could be altered by burning (not burned, or burned in either early, mid-, or late spring) and applying fertilizer (0 and 67-23 kg N-P ha-1) and atrazine [6- chloro-N-ethyl-N'-(1-methylethyl)-1,3,5-triazine-2,4-diamine] (0 and 2.2 kg a.i. ha-1). Vegetation was harvested the year treatments were applied at about 30-day intervals starting in June and ending …


Harvest Frequency And Burning Effects On Monocultures Of 3 Warm-Season Grasses, Gregory J. Cuomo, Bruce Anderson, Linda J. Young, Wally Wilhelm Mar 1996

Harvest Frequency And Burning Effects On Monocultures Of 3 Warm-Season Grasses, Gregory J. Cuomo, Bruce Anderson, Linda J. Young, Wally Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Harvest frequency and burning can affect forage yield of monocultures of switchgrass (Panicurn virgutum L.), big bluestem (Andropogon gerurdii Vitman), and Indian grass [Sorghastrum nutuns (L.) Nash]. Current information is based largely on results from mixed stands. A field experiment was established in 1986, and from 1988 to 1991 treatments were applied with burning in March, April, or May plus an unburned control. Growing season yield was measured by harvesting 1 (June), 2 (June and July), or 3 (June, July, and August) times with unharvested control plots included. End-of-season standing crop from all plots was determined …


Semiochemistry Of Cabbage Bugs (Heteroptera: Pentatomidae: Eurydema And Murgada, Jeffrey R. Aldrich, James W. Avery, Chang-Joo Lee, Jennifer C. Graf, Dawn J. Harrison, Ferdinando Bin Jan 1996

Semiochemistry Of Cabbage Bugs (Heteroptera: Pentatomidae: Eurydema And Murgada, Jeffrey R. Aldrich, James W. Avery, Chang-Joo Lee, Jennifer C. Graf, Dawn J. Harrison, Ferdinando Bin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The semiochemistry of the common North American pest of crucifers, Murgantia histrionica (Hahn) (the harlequin bug), and two related European species, Eurydema ventrale L. and E. oleraceum L., was investigated.


Imidazolinone Herbicides Improve Restoration Of Great Plains Grasslands, Robert A. Masters, Scott Nissen, Roch E. Gaussoin, Daniel Beran, Robert Stougaard Jan 1996

Imidazolinone Herbicides Improve Restoration Of Great Plains Grasslands, Robert A. Masters, Scott Nissen, Roch E. Gaussoin, Daniel Beran, Robert Stougaard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The productivity and native species diversity of Great Plains grasslands have been substantially reduced by past management that facilitated the establishment of invasive exotic weeds and displacement of native species. Management strategies are needed to rapidly restore the productive capacity and biological diversity of these degraded grasslands. Critically important phases of the grassland restoration process are the reintroduction and establishment of native species. Weed interference is the primary constraint to successful establishment of native plants. The goal of our research is to develop strategies that use multiple technologies, including herbicides, to expedite grassland revegetation with native grasses and forbs. Imidazolinon …


Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters Jan 1996

Leafy Spurge (Euphorbia Esula) Genotype Affects Gall Midge (Spurgia Esulae) Establishment, Rodney G. Lym, Scott Nissen, Martha Rowe, Donald J. Lee, Robert A. Masters

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Greenhouse cage studies were conducted to determine the influence of shoot morphology and genetic variation on establishment of Spurgia sulae gall midge on seven leafy spurge genotypes. The genotypes were collected from South Dakota, North Dakota, Nebraska, Montana, Wyoming, Manitoba, and Austria. Genotypes from South Dakota and Nebraska were most susceptible to gall formation and had the highest larvae survival, while the genotypes from Montana and Manitoba were most resistant. Morphological characteristics of the leafy spurge stem tips, such as stem diameter leaf, length, width, and area did not correlate with gall formation or larvae survival. Chloroplast DNA restriction fragment …


Permethrin-Impregnated Yarn: Longevity Of Efficacy And Potential Use On Cylindrical Fiberglass Stable Fly (Diptera: Muscidae) Traps, Jerome Hogsette, Joseph Ruff Jan 1996

Permethrin-Impregnated Yarn: Longevity Of Efficacy And Potential Use On Cylindrical Fiberglass Stable Fly (Diptera: Muscidae) Traps, Jerome Hogsette, Joseph Ruff

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Efficacy of permethrin-impregnated orlon yam against stable flies, Stomoxys calcitrans (L.), was 6-8 wk under simulated field conditions. Numbers of stable flies captured on corrugated cylindrical fiberglass traps painted with an adhesive indicated that these traps are attractive enough to be used in the field with treated yam applied. Reasons for variability of yam impregnation and the use of yam on cylinder traps are discussed.


Carbonate And Gypsum, Richard H. Loeppert, Donald L. Suarez Jan 1996

Carbonate And Gypsum, Richard H. Loeppert, Donald L. Suarez

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The carbonate minerals and gypsum exert a dominating influence on soils in
which they are present because of their relatively high solubility, and in the case
of the carbonates, their alkalinity and pH buffering properties. In this chapter,
procedures for the determination of quantity, reactivity and equilibrium relations
of these minerals will be discussed.


Development Of House Flies (Diptera: Muscidae) In Sand Containing Varying Amounts Of Manure Solids And Moisture, Jerome Hogsette Jan 1996

Development Of House Flies (Diptera: Muscidae) In Sand Containing Varying Amounts Of Manure Solids And Moisture, Jerome Hogsette

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House flies, Musca domestica L., developed in 200 cm3 of coarse sand containing just 1 ml (0.47%) of dairy manure solids and 10 ml (4.74%) of moisture. At these levels, development was slow (21.5 d from 1st instar to adult), adult survival was low (7.5%), but successful development did occur. At higher manure and moisture levels, rates of development and survival were similar to those reported previously. All soil samples collected from a feedlot dairy contained higher levels of manure solids than the highest level tested in the laboratory. The implications for fly control in soil/manure mixtures and the …