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Comparative Studies On The Competence Of Axillary Shoot Regeneration On Unsliced And Longitudinally Sliced Cotyledon Nodes Of Vigna Unguiculata, Muhammad Aasim, Sancar Fati̇h Özcan, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Comparative Studies On The Competence Of Axillary Shoot Regeneration On Unsliced And Longitudinally Sliced Cotyledon Nodes Of Vigna Unguiculata, Muhammad Aasim, Sancar Fati̇h Özcan, Khalid Mahmood Khawar, Sebahatti̇n Özcan
Turkish Journal of Botany
Vigna unguiculata is an important food legume crop in the semiarid tropics. It suffers from a host of agricultural constraints including damage due to diseases and pests. Judicious application of biotechnological methods can lead to considerable improvement in this important crop. Shoot regeneration from unsliced and longitudinally sliced cotyledon node explants obtained from 3-day-old to 5-day-old seedlings grown in vitro was achieved on Murashige and Skoog (MS) medium containing 1.11, 2.22, 3.33, and 4.44 \muM benzylaminopurine (BA) supplemented with 1.0 mg/L polyvinylpyrrolidone and 500 mg/L bacteriostatic Augmentin. Callus induction was recorded in all culture media on both explants. The shoot …