Digital Commons Network^™

The Virome Of Peony And The Population Structure Of Its Most Prominent Viruses, Cullen Shaffer

Graduate Theses and Dissertations

Peony (Peonia lactiflora, Pall.) is a popular ornamental that has been cultivated for millennia. Due to its popularity, plant material is frequently moved across international borders allowing for the spread of viruses. The virome of several peony plants was investigated and four viruses; namely Amazon lily mild mottle virus (ALiMMV), Cycas necrotic stunt virus (CNSV), Gentian Kobu-sho associated virus (GKaV) and Lychnis mottle virus (LycMoV) were detected for the first time in the Western Hemisphere. Incidence ranged from a few plants for ALiMMV to near universal infection for CNSV. GKaV was found in individuals that were infected with Lemoine’s disease …

Parallel Strategies To Control Bacterial Panicle Blight Of Rice, Laura Ortega

Graduate Theses and Dissertations

Bacterial Panicle Blight (BPB) of rice is a seed-borne disease caused by the bacterium Burkholderia glumae. This disease has affected rice production worldwide and its effects are likely to become more devastating with the continuous increase in global temperatures especially during the growing season. Field data has shown that the disease has been more prevalent in years when the temperatures have been unusually high especially at night possibly due to the bacterial adaptation to grow at temperatures higher than 40oC. With the continuous rise in global temperatures, it is likely that this disease will be more problematic. The bacterium can …

Optimization Of In-Vessel Food Waste Composting: Enzyme Activity And Microbial Dynamics, Ayawovi Selom Ametepe

Graduate Theses and Dissertations

A series of greenhouse-based, rotary-drum bioreactor experiments was designed to study microbial dynamics and enzyme activity during optimization of food waste composting. This work aims to optimize food waste composting by defining the best food waste-to-bulking agent proportion controlling conditions and by evaluating the food waste composting process when inoculated by a bacterial inoculant product compared to uninoculated compost. Three experiments were run in total. The two first experiments were conducted for 48 days with sampling at each step of composting, while the third experiment last 50 days and included one extra sampling date. In the first experiment, 50:50, 65:35 …

Characterization Of Genetic Sources Associated With Restorability And Seed Dimension In Arkansas Restorer Rice Lines, Ozgur Azapoglu

Graduate Theses and Dissertations

Hybrid rice (Oryza sativa L.) breeding offers a significant opportunity to enhance rice production, and the cultivation of a male sterile line is the most important factor in the success of cross-breeding. One of the key elements of hybrid rice production is to develop a restorer line that is assigned as the male parent. The restorer lines provide viable pollen for fertilization of the male sterile plant due to the presence of a restorer gene (Rf) in their genomes. Any superior restorer line applied to hybrid rice production must contain genes/quantitative trait loci (QTL) associated with the desirable agronomic traits …

Application Of X-Ray Imaging And Glass Transition Principles In Understanding Rice Kernel Fissure Formation, Zephania R. Odek

Graduate Theses and Dissertations

Rice kernels with internal fractures of the endosperm, fissures, tend to break during milling, leading to head rice yield (HRY) reductions. Minimizing kernel fissuring is thus an important goal of the rice industry. To minimize fissuring during the drying process, a better understanding of the fissuring process and the kinetics thereof is required. The goal of this research was to use X-ray imaging and glass transition principles to better understand rough rice kernel fissuring. First, because the instance when the fissuring process is initiated and completed during the drying process is not well known, an X-ray imaging setup that allows …

Association Mapping For Soybean (Glycine Max L. Merr.) Protein And Oil Content, Joseph Najjar

Graduate Theses and Dissertations

Soybean [Glycine Max (L.) Merrill] is an important economic crop because of its high content of seed protein and oil. Seed oil and protein content are quantitative inherited traits. The genetics of seed protein and oil levels have been extensively studied, with 367 QTL reported for protein and 475 QTL reported for oil to date. Validation of such QTLs, and identification of easily-automatable molecular markers around these QTL will aid the progression of breeding for such traits. The focus of this research was to discover novel and verify previously reported QTL related to protein and/or oil content via Genome-Wide Association …

Comparison Of A T-Trellis And A Rotating Cross-Arm Trellis For Arkansas Blackberry Production, Erika Henderson

Graduate Theses and Dissertations

As blackberry production in the southeastern United States expands, the need for updated cultural practices, like alternative trellis systems that enhance blackberry fruit yield, fruit quality, and economic viability for fresh-market growers are needed. There are challenges to producing high-quality blackberry fruit with high yields in the Southeast due to intense heat, high rainfall, humidity, and heavy pest pressure. Southeastern growers are interested in the rotating cross-arm (RCA) trellis due to its potential to increase yield and fruit quality, however there is minimal research of its use in blackberry production in the Southeast. In 2019 and 2020, a trial was …

Rice Chilling Aeration: Assessment Of Impacts On Grain Quality, Process Modeling And Validation, Soraya Shafiekhani

Graduate Theses and Dissertations

The goal of this study is to demonstrate the effectiveness of applying chilling temperatures during on-farm, in-bin drying and storage to maintain rice quality. Specific objectives include to demonstrate the effectiveness of chilling aeration to preserve rice quality characteristics, determine the drying kinetics of rice under low temperature environments, and develop models to predict heat and mass transfer in rice kernel during drying at low temperature environment. To date, there is limited research describing storability or drying behavior of rice in chilled environments. However, it is understood that chilling of high-moisture, > 21% wet basis rough rice could reduce the time …

Impacts Of Postharvest Temperature Exposure Profiles On Rice Physicochemical Properties, Seth Graham-Acquaah

Graduate Theses and Dissertations

Heated-air drying followed by tempering (HAT) is effective for increasing rough-rice drying rates without compromising head rice yield (HRY). However, heat exposure could affect rice end-use properties. Hypothesizing that the total amount of heat exposure incurred by rice during heated-air drying determines the trend and magnitude of changes in end-use properties, this dissertation sought to 1) characterize the effects of drying and tempering regimen on changes in end-use properties, 2) derive an index to quantify and compare the amount of heat exposure that rice kernels incur during active drying and, 3) relate values of this index to changes in rice …

Improving Breeding Program Efficiency And Genetic Gain Through The Implementation Of Genomic Selection In Diverse Wheat Germplasm, Dylan Larkin

Graduate Theses and Dissertations

Genomic selection (GS) is an important tool for increasing genetic gain for economically important traits in breeding programs. Genomic selection uses molecular markers across the entire genome in order to predict the performance of breeding lines for a trait of interest prior to phenotyping. A training population (TP) of elite germplasm, representative of the University of Arkansas wheat breeding program, was developed in order to predict important agronomic and Fusarium head blight (FHB) resistance traits within the University of Arkansas wheat breeding program through cross-validation and forward prediction.

A genome-wide association study (GWAS) was performed on the TP to identify …

Molecular Genetic Analysis Of Drought Resistance And Productivity Traits Of Rice Genotypes, Yheni Dwiningsih

Graduate Theses and Dissertations

Rice (Oryza sativa L.) is the staple food for a majority of the world’s population, and uses 30% of the global fresh water during its life cycle. Drought at the reproductive stage is the most important abiotic stress factor limiting grain yield. The United States is the third largest exporter of rice, and Arkansas is the top rice-producing state. The Arkansas rice-growing region in the Lower Mississippi belt is among the 10 areas with the highest risk of water scarcity. Adapted U.S. rice cultivars were screened for drought resistant (DR) traits to find sources for breeding U.S. rice cultivars for …

Resistance To Herbicides Conferred By Amaranthus Palmeri Protoporphyrinogen Ix Oxidase Mutations, Pamela Carvalho De Lima

Graduate Theses and Dissertations

Palmer amaranth (Amaranthus palmeri S. Wats.) is one of the most problematic agronomic weeds to control in fields across Arkansas. Thus far, this species has evolved resistance to several herbicides, including protoporphyrinogen IX oxidase (PPO) inhibitors. The majority of PPO-resistant Palmer amaranth populations harbor a target-site mutation (substitution or deletion of amino acids). The objective of this thesis was to identify the level of fomesafen resistance conferred by PPO2 mutations from Palmer amaranth. The experiments conducted aimed to (1) characterize the level of resistance conferred by the transgene Palmer amaranth ppo2 carrying ΔG210 mutation into the wild type rice (Oryza …

Taxonomic And Genetic Diversity, And Pathogenicity Of Diaporthe Species Associated With Soybean, Fakhir Eraheem Hameed Al Shuwaili

Graduate Theses and Dissertations

Diaporthe species (anamorph: Phomopsis) are associated with a wide range of plant hosts as plant pathogens, asymptomatic endophytes, and saprobes. One of these hosts is soybean, which is one of the most important crops in U.S. agriculture. Several Diaporthe species cause important diseases on soybean in the U.S., and specifically in Arkansas. The taxonomy, genetic diversity, and pathogenicity of Diaporthe species associated with asymptomatic infection of soybean are rarely studied with accurate molecular tools. Therefore, this dissertation aimed to assess the diversity and boundaries of Diaporthe associated with soybean in Arkansas. Furthermore, pathogenicity and alternative lifestyles were assessed among Diaporthe …

Dissecting The Cellular Control Of Septin Organization In A Global Cereal Killer, Nawaraj Dulal

Graduate Theses and Dissertations

Rice blast disease, caused by the filamentous fungus Magnaporthe oryzae, destroys sufficient rice each year to feed 60 million people, and is a serious threat to global food security. A wheat-adapted lineage of M. oryzae now poses threat to global wheat production. Rice blast disease is currently controlled using limited fungicides, and the emergence of fungicide resistance within M. oryzae populations is a growing concern. There is a pressing need to identify new classes of fungicides to control the disease, which requires better understanding of the basic biology of the pathogen. To establish disease, M. oryzae forms a specialized dome …

Performance Evaluation Of Four Arkansas Table Grape Cultivars Grown On Three Trellis Systems Under High Tunnels At Two Locations In Arkansas., Jose Hernandez

Graduate Theses and Dissertations

Grapevines (Vitis vinifera L.) are one of the most important horticultural crops world-wide. In the southern U.S. region, hot and humid climate accompanied by high pest pressure requires high levels of labor and pesticide inputs, making open field table grape production unsustainable. Previous research at University of Arkansas has demonstrated that table grape production under high tunnels (HTs) is a viable option to overcome biotic and abiotic challenges in the region. This project evaluated two HTs established in Arkansas. In the spring of 2014, at Fayetteville, three table grape cultivars (Faith, Gratitude, and Jupiter) were established in an 8 x …

Genetic Architecture Of Salt And Drought Tolerance In Cowpea, Waltram Second Ravelombola

Graduate Theses and Dissertations

Cowpea [Vigna unguiculata (L.) Walp.] is a diploid and nutrient-dense legume species. It provides affordable source of protein to human. Cowpea cultivation is prevalent in Africa, Asia, the western and southern U.S., and Central and South America. However, earlier reports have shown that drought and salt stress can be devastating to cowpea production. The objectives of this study were to screen for salt and drought tolerance in cowpea and to identify molecular markers associated with these traits. Simple methodologies to screen for drought (Chapter 2) and salt tolerance were developed (Chapter 3). Results suggested that: 1) a total of 14, …

Introducing An Indigenous Non-Toxigenic Aspergillus Flavus Strain Isolated From Iraqi Corn Grains As A Bio-Control Agent To Reduce Aflatoxin Contamination In Corn Grains, Ali Almatakeez

Graduate Theses and Dissertations

Mycotoxin contamination of cereal crops, such as maize and sorghum, is a global concern because of the potential health effects on humans and animals. Although substantial research has been conducting regarding mycotoxin prevention and mitigation, little information is available about the association of mycotoxin-producing fungi with corn and sorghum grain in Iraq. Identifying and refining indigenous atoxigenic strains to reduce mycotoxin contamination of maize and sorghum has the potential to enhance the nutritional value of these grains while reducing economic losses. However, to our knowledge, this tactic has not yet been adopted by agricultural authorities and farmers in Iraq. To …

Genetics Of Physiological Traits Associated With Drought Tolerance In Soybean (Glycine Max), Sumandeep Kaur Bazzer

Graduate Theses and Dissertations

Soybean (Glycine max L.) is one of the major row crops in the United States, and its production is often limited by drought stress. Physiological traits from exotic germplasm that confer drought tolerance may be useful in improving commercial soybean production. For example, carbon isotope ratio (δ13C) is positively correlated with water use efficiency (WUE), and nitrogen isotope ratio (δ15N) is negatively correlated with N2 fixation; canopy temperature (CT) is an indicator for genetic variation in transpiration and stomatal conductance. Therefore, the objectives of this research were to identify the genomic regions associated with: (1) δ13C and δ15N using a …

Developing A High-Throughput Method To Screen Soybean Germplasm For Hypoxia Tolerance In Hydroponic Systems, Derrick Harrison

Graduate Theses and Dissertations

Soybean [Glycine max (Merr.)], the second most planted crop in the United States, is sensitive to field flooding due to depletion of oxygen and accumulation of CO2 in the rhizosphere. There is a need to breed cultivars that are adapted to areas prone to flooding, but field evaluations in the U.S. are limited because of time (one generation per year) and availability of resources (impermeable soils, irrigation, equipment to build levees). The purpose of this study was to develop and execute a protocol of germplasm screening for hypoxia tolerance using hydroponics in a controlled greenhouse environment. Germination rates and vigor …

Phenotypic And Genotypic Studies In The Peach [Prunus Persica (L.) Batsch] And Muscadine Grape (Vitis Rotundifolia Michx.), Jennifer Anne Lewter

Graduate Theses and Dissertations

Peach: Peaches [Prunus persica (L.) Batsch] are routinely chilled to increase shelf-life. Exposure to temperatures of 5° C for two weeks can induce chilling injury (CI) symptoms, including flesh mealiness (or wooliness) and a lack of juiciness. Phenotypic data were collected on seven biparental F1 peach populations maintained at the University of Arkansas Fruit Research Station. A genome wide association study (GWAS) was performed using TASSEL 5 which identified four quantitative trait loci (QTLs) associated with expressible juice, four QTLs for mealiness, five QTLs for soluble solids, and three QTLs for fruit weight. Exploiting these genetic markers could help breeders …

Sensitivity Of Enlisttm And Roundup Ready Xtend® Technologies To Auxin Herbicides And Comparison Of Resistance To Susceptible Cotton And Soybean Cultivars, James Rose

Graduate Theses and Dissertations

Increases in weed resistance have led to the development of new herbicide-resistant crop technologies, namely the Roundup Ready Xtend® and EnlistTM weed control systems. These new technologies provide resistance to applications of the synthetic auxins dicamba or 2,4-D, respectively, in traited cotton or soybean. It is unknown if there is any resistance to other herbicides within the synthetic auxin group (WSSA Group 4) in these traited crops. Experiments were conducted with auxin herbicides representing all five families within WSSA Group 4. Herbicides were applied either preemergence (PRE) or postemergence (POST) relative to the crop in a single application. Applications were …

Hydroponic And Soilless Culture Systems And Transplant Practices Influence Production Of Basil (Omicum Basilicum L.), Samuel Warren Edward Doty

Graduate Theses and Dissertations

Two controlled-environment greenhouse experiments evaluated (1) plant growth and morphology of basil (Omicum basilicum L.) grown in three different hydroponic and soilless substrate systems and (2) seedling tray cell-count and transplant date into hydroponic culture effects on basil growth and yield at harvest. For the first experiment, four basil cultivars (‘Genovese’, ‘Mrs. Burns Lemon’, ‘Sweet Thai’, and ‘Cinnamon’) were grown in deep flow technique (DFT) hydroponics, nutrient film technique (NFT) hydroponics, and a novel shallow aggregate ebb-and-flood (SAEF) soilless substrate system and were harvested after 21 d. Plant yield and morphological characteristics measured at harvest included shoot fresh mass, shoot …

Techniques To Enhance The Attributes Of Wines Produced From Grapes Grown In Arkansas, Sarah Mayfield

Graduate Theses and Dissertations

Grapevines (Vitis spp.) are one of the most widely-planted horticultural crops, and the United States plays a major role in grape and wine production. Arkansas has a long history of grape and wine production with grapes grown in Arkansas including mostly native species, such as muscadines, and hybrids (crosses of Vitis spp.), such as Chambourcin. In addition, the University of Arkansas System Division of Agriculture (UA System) grape breeding program has cultivars and selections that have shown potential for wine production. The objectives of this research were to: evaluate effects of specific inactivated yeast application to Chambourcin grapevines on attributes …

Mapping Quantitative Trait Loci For Peach (Prunus Persica) Resistance To Xanthomonas Arboricola Pv. Pruni (Xap) And Determining The Diversity And Virulence Of A United States Xap Collection, Maxwell Vonkreuzhof

Graduate Theses and Dissertations

Bacterial spot, caused by the bacterium Xanthomonas arboricola pv. pruni (Xap), is a threat to the peach [Prunus persica (L.) Batsch], Japanese and European plum (P. salicina L. and P. domestica L.), and tart and sweet cherry (P. cerasus L. and P. avium L.) industries. Markers for fruit resistance to bacterial spot have been developed however, markers associated with foliar resistance have yet to be developed. A total of 130 progeny and 13 parents (n=143) were evaluated for foliar and fruit Xap resistance in 2013, 2014, and 2015, and 162 progeny and eight parents (n=170) in 2017 and 2018 as …

A Comparative Ecological Study Of Limestone And Dolomite Glades In The Ozark Mountains Of Northwest Arkansas, Brittney Booth

Graduate Theses and Dissertations

Glades are one of the many habitats that exist in the Arkansas Ozarks and contribute to the overall biodiversity of the state of Arkansas. For this study, five dolomite glades and five limestone glades in the Ozarks of northwest Arkansas were studied from March to October in the years 2017 and 2018 to determine the similarities or differences that might be present. One hundred and fifteen vascular plant taxa were documented in the dolomite glades and one hundred and three vascular plant taxa were documented in the limestone glades. Forty-six vascular plant taxa were unique to the dolomite glades and …

Elucidating Basis Of Rice Discoloration And Developing Prevention Strategies, Zeinab Mohammadi Shad

Graduate Theses and Dissertations

Economically, discolored rice kernels have less market value, which can seriously impact farm net profit. The reasons for rice discoloration during storage have not been studied extensively and many questions remain. Therefore, the primary goal of this study was to improve understanding of the role and contributions of storage practices on rice quality degradation and discoloration. In this study, three rice cultivars including XL753 (hybrid), Roy J (pureline), and Titan were assessed for changes in quality and microbial kinetics. The rice samples at a high moisture content (MC) of 21% w.b. and a low MC of 16% were stored at …

Phylogenetic Diversity Of Arkansas Vineyard And Wine Microbiota, Natacha Cureau

Graduate Theses and Dissertations

Microorganisms inhabiting the soil, leaves, and grapes in vineyards influence the terroir, a set of environmental factors that impact wine characteristics. Previously, the main method to identify microorganisms was to culture on different media, but a large portion (> 99%) could not be cultured and were consequently unidentified. However, the emergence of new molecular tools has enabled further study and identification of microorganisms such as indigenous vineyard microbiota. The objective of this research was to investigate the phylogenetic diversity of Arkansas vineyard and wine microbiota using high-throughput sequencing. In terms of the Arkansas vineyards, the fungal and bacterial diversity of …

Determination Of Critical Y-Leaf Potassium Concentrations Across Reproductive Growth Stages For Direct-Seeded Delay-Flooded Rice, Chandler Edwin Gruener

Graduate Theses and Dissertations

Rice (Oryza sativa L.) grain yield can be limited by potassium (K) deficiency on soils low in exchangeable K. Visually diagnosing K deficiency during early reproductive growth is not easily done and the interpretation of traditional tissue analysis is limited to select growth-stages. Our primary focus was to define continuous critical Y-leaf-K concentrations during reproductive growth for the production of maximal grain yield. A secondary objective was to examine Y-leaf sap-K concentration, measured using a handheld device, as a rapid in-field method of monitoring rice plant K nutrition. The Y-leaf is defined as the uppermost fully extended leaf with a …

An Evaluation Of Biopesticide Combinations On Yield Performance And Disease/Arthropod Control Of Strawberries Grown In High Tunnel Plasticulture Production Systems In Arkansas., Karlee B. Pruitt

Graduate Theses and Dissertations

This two-year study investigated combinations of biopesticides to determine impacts on strawberry fruit marketable fruit yields, and effectiveness in controlling strawberry pests in a high tunnel production system at the University of Arkansas, Division of Agriculture Research and Extension Center in Fayetteville, Arkansas. Two strawberry cultivars Fragaria × ananassa (Duch.), Camino Real and Sweet Sensation were grown in a high tunnel from early-October to mid-May for two consecutive growing seasons, (2017-18 and 2018-19) with six treatment combinations of biopesticides including an untreated (water) control, nutrient spray and selected biological based fungicides and insecticides, arranged into a split-plot randomized block design. …

Florpyrauxifen-Benzyl Use In Arkansas Rice (Oryza Sativa L.), Hannah Elizabeth Wright

Graduate Theses and Dissertations

Florpyrauxifen-benzyl is a synthetic auxin herbicide which was commercially released in 2018 to combat troublesome grass, broadleaf, and sedge weed species in rice. Many factors may influence cultivar response to a new herbicide; hence, it is important to understand factors contributing to crop sensitivity to an herbicide in order to make appropriate recommendations. Prior to the onset of this study, research had been conducted on florpyrauxifen-benzyl in a flooded environment; however, none had been executed in a non-flooded environment. Experiments were conducted to evaluate the response of a long-grain variety ‘CL111’, a medium-grain variety ‘CL272’, and a long-grain hybrid ‘CLXL745’ …