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Carbon Sequestration And Weedy Invasion In Crp Fields, Destiny Danielle Magee May 2020

Carbon Sequestration And Weedy Invasion In Crp Fields, Destiny Danielle Magee

Dissertations and Theses @ UNI

The Conservation Reserve Program (CRP) aims to provide ecosystem services in landscapes dominated by agriculture by converting previous agricultural fields into reconstructed prairies and wetlands. The main priorities of the program include reducing soil erosion and improving water quality, but the perennial vegetation in the fields can also provide other benefits such as increased carbon sequestration and biodiversity. These additional benefits of the program are not closely monitored, though these benefits are potentially equally valuable to the targeted program benefits. This study compared soil carbon in CRP fields and a subset of corn fields in order to determine the impact …


Productivity And Community Composition Change In Prairie Biomass Feedstocks, Kathleen Loretta Madsen May 2020

Productivity And Community Composition Change In Prairie Biomass Feedstocks, Kathleen Loretta Madsen

Dissertations and Theses @ UNI

With increasing concerns of climate change and resource usage, there is a growing interest in diverse prairie biomass as a form of alternative energy. In this study, we look at four potential biomass feedstock treatments that were chosen specifically to target high biomass yields and consist of native Iowa tallgrass prairie species. The four feedstocks consist of a one-species monoculture of C4 switchgrass (Panicum virgatum L.), a 5-species mixture of C4-grasses, a 16-species mixture of C3 and C4-grasses, forbs, and legumes, and a 32-species mixture of C3 and C4-grasses, sedges, forbs, and …


Evaluation Of The Csr1-2 Allele From Arabidopsis Thaliana Cs3102 As An Imazapyr Herbicide Resistance Marker For Biolistic Transformation Of The Shoot Apical Meristem, Amanda J. Stump Jan 2020

Evaluation Of The Csr1-2 Allele From Arabidopsis Thaliana Cs3102 As An Imazapyr Herbicide Resistance Marker For Biolistic Transformation Of The Shoot Apical Meristem, Amanda J. Stump

Dissertations and Theses @ UNI

Plant genetic engineering requires a marker gene to differentiate transformed cells. To test the csr1-2 allele as a selectable marker for the shoot apical meristem (SAM), we cloned a genomic fragment from Arabidopsis thaliana mutant CS3102 which contained the coding sequence and promoter. We then evaluated the utility of this allele on two model systems, Arabidopsis and tobacco, with soybean as the positive control. Initial dose-response experiments were conducted using a seedling plate assay to determine the lethal dose of imazapyr. For the mutant Arabidopsis thaliana CS3102, a concentration of 500 μM imazapyr was required to overcome the resistance phenotype …